Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

During leaf development, the timing of transition from cell proliferation to expansion is an important factor in determining the final organ size. However, the regulatory system involved in this transition remains less understood. To get an insight into this system, we investigated the compensation phenomenon, in which the cell number decreases while the cell size increases in organs with determinate growth. Compensation is observed in several plant species suggesting coordination between cell proliferation and expansion. In this study, we examined an Arabidopsis mutant of ANGUSTIFOLIA 3 (AN3)/GRF-INTERACTING FACTOR 1, a positive regulator of cell proliferation, which exhibits the compensation. Though the AN3 role has been extensively investigated, the mechanism underlying excess cell expansion in the an3 mutant remains unknown. Focusing on the early stage of leaf development, we performed kinematic, cytological, biochemical, and transcriptome analyses, and found that the cell size had already increased during the proliferation phase, with active cell proliferation in the an3 mutant. Moreover, at this stage, chloroplasts, vacuoles, and xylem cells developed earlier than in the wild-type cells. Transcriptome data showed that photosynthetic activity and secondary cell wall biosynthesis were activated in an3 proliferating cells. These results indicated that precocious cell differentiation occurs in an3 cells. Therefore, we suggest a novel AN3 role in the suppression of cell expansion/differentiation during the cell proliferation phase.

File： Ezaki-et-al_2024.pdf

DOI： 10.3389/fpls.2024.1322223

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File： Sakakibara_ezaki_20214_BSJ-Review_15B_74-80.pdf

DOI： 10.24480/bsj-review.15b6.00264

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Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science ({PLoS})  

File： 2020_PlosGenetics.pdf

DOI： 10.1371/journal.pgen.1008873

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Authorship：Lead author   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Society of Plant Morphology  

File： 江崎・塚谷_分裂組織における細胞サイズの制御機構.pdf

DOI： 10.5685/plmorphol.32.45

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Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

Abstract

Background and aims

Domatia are plant structures within which organisms reside. Callicarpa saccata (Lamiaceae) is the sole myrmecophyte, or ‘ant plant’, that develops foliar (leaf-borne) myrmeco-domatia in this genus. In this work we examined domatium development in C. saccata to understand the developmental processes behind pouch-like domatia.

Methods

Scanning electron microscopy, sectioning and microcomputed tomography were carried out to compare the leaves of C. saccata with those of the closely related but domatia-less myrmecophyte Callicarpa subaequalis, both under cultivation without ants.

Key results

Callicarpa saccata domatia are formed as a result of excess cell proliferation at the blade/petiole junctions of leaf primordia. Blade/petiole junctions are important meristematic sites in simple leaf organogenesis. We also found that the mesophyll tissue of domatia does not clearly differentiate into palisade and spongy layers.

Conclusions

Rather than curling of the leaf margins, a perturbation of the normal functioning of the blade/petiole junction results in the formation of domatium tissue. Excess cell proliferation warps the shape of the blade and disturbs the development of the proximal–distal axis. This process leads to the generation of distinct structures that facilitate interaction between C. saccata and ants.

File： 2020_Callicarpa.pdf

DOI： 10.1093/aob/mcz193

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Other Link： http://academic.oup.com/aob/article-pdf/125/3/521/32853849/mcz193.pdf