-
Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation
Hironobu Fujita, Ayane Osaku, Yuto Sakane, Koki Yoshida, Kayoko Yamada, Seia Nara, Takahito Mukai, Masayuki Su’etsugu
ACS Synthetic Biology
23 8 2022
-
In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
Seia Nara, Masayuki Su'etsugu
BioTechniques (Peek behind the paper selected)71
(
4
)
528
-
533
10 2021
-
Amplification of over 100 kbp DNA from Single Template Molecules in Femtoliter Droplets.
International journal
Hiroshi Ueno, Hiroki Sawada, Naoki Soga, Mio Sano, Seia Nara, Kazuhito V Tabata, Masayuki Su'etsugu, Hiroyuki Noji
ACS synthetic biology10
(
9
)
2179
-
2186
17 9 2021
-
Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes.
International journal
Tatsuya Yoneji, Hironobu Fujita, Takahito Mukai, Masayuki Su'etsugu
Nucleic acids research (Breakthrough paper selected)49
(
15
)
8407
-
8418
28 4 2021
-
Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli.
International journal
Takahito Mukai, Tatsuya Yoneji, Kayoko Yamada, Hironobu Fujita, Seia Nara, Masayuki Su'etsugu
ACS synthetic biology9
(
6
)
1315
-
1327
19 6 2020
-
Cell-Penetrating Peptide-Mediated Transformation of Large Plasmid DNA into Escherichia coli
Peer-reviewed
Islam M.M., Odahara M., Yoshizumi T., Oikawa K., Kimura M., Su'etsugu, M. and Numata K.
ACS Synth. Biol.8
(
5
)
1215
-
1218
4 2019
-
Efficient Arrangement of the Replication Fork Trap for In Vitro Propagation of Monomeric Circular DNA in the Chromosome-Replication Cycle Reaction
Hasebe, T, Narita, K, Hidaka, S, Su'etsugu, M
Life
9 2018
-
Bacterial EndoMS/NucS acts as a clamp-mediated mismatch endonuclease to prevent asymmetric accumulation of replication errors
Takemoto, N, Numata, I, Su'etsugu, M, Miyoshi-Akiyama, T, co, s, ondi
Nucleic Acids Res.
6 2018
-
Essentiality of walRK for growth in bacillus subtilis and its role during heat stress
Hiraku Takada, Yuh Shiwa, Yuta Takino, Natsuki Osaka, Shuhei Ueda, Satoru Watanabe, Taku Chibazakura, Masayuki Su’Etsugu, Ryutaro Utsumi, Hirofumi Yoshikawa
Microbiology (United Kingdom)164
(
4
)
670
-
684
1 4 2018
-
Exponential propagation of large circular DNA by reconstitution of a chromosome-replication cycle
Su'etsugu, M, Takada, H, Katayama, T, Tsujimoto, H
Nucleic Acids Res.
9 2017
-
Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts
Katsuhiko Kamada, Masayuki Su'etsugu, Hiraku Takada, Makoto Miyata, Tatsuya Hirano
STRUCTURE25
(
4
)
603
-
+
4 2017
-
DnaA N-terminal domain interacts with Hda to facilitate replicase clamp-mediated inactivation of DnaA
Peer-reviewed
Su'etsugu, M, Harada, Y, Keyamura, K, Matsunaga, C, Kasho, K, Abe, Y, Ueda, T, Katayama, T
Environ. Microbiol.
7 2013
-
The Replicase Sliding Clamp Dynamically Accumulates behind Progressing Replication Forks in Bacillus subtilis Cells
Peer-reviewed
Masayuki Su'etsugu, Jeff Errington
MOLECULAR CELL41
(
6
)
720
-
732
3 2011
-
Hda monomerization by ADP binding promotes replicase clamp-mediated DnaA-ATP hydrolysis
Peer-reviewed
Su'etsugu, M, Nakamura, K, Keyamura, K, Kudo, Y, Katayama, T
J. Biol. Chem.
7 2008
-
Modes of over-initiation, dnaA gene expression and the inhibition of cell division in a novel cold-sensitive hda mutant in Escherichia coli.
Peer-reviewed
Fujimitsu, K, Su'etsugu, M, Yamaguchi, Y, Mazda, K, Fu, N, Kawakami, H, Katayama, T
J. Bacteriol.
3 2008
-
The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP-DnaA-specific initiation complexes
Peer-reviewed
Kenji Keyamura, Norie Fujikawa, Takuma Ishida, Shogo Ozaki, Masayuki Su'etsugu, Kazuyuki Fujimitsu, Wataru Kagawa, Shigeyuki Yokoyama, Hitoshi Kurumizaka, Tsutomu Katayama
GENES & DEVELOPMENT21
(
16
)
2083
-
2099
8 2007
-
The exceptionally tight affinity of DnaA for ATP/ADP requires a unique aspartic acid residue in the AAA+ sensor 1 motif
Peer-reviewed
Kawakami, H, Ozaki, S, Suzuki, S, Nakamura, K, Senriuchi, T, Su'etsugu, M, Fujimitsu, K, Katayama, T
Mol. Microbiol.
8 2006
-
An isolated Hda–clamp complex is functional in the regulatory inactivation of DnaA and DNA replication
Peer-reviewed
Kawakami, H, Su'etsugu, M, Katayama, T
J. Struct. Biol.156
220
-
229
8 2006
-
Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation (vol 59, pg 265, 2005)
Peer-reviewed
T Ote, M Hashimoto, Y Ikeuchi, M Su'etsugu, T Suzuki, T Katayama, JI Kato
MOLECULAR MICROBIOLOGY60
(
1
)
252
-
252
4 2006
-
Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation
Peer-reviewed
T Ote, M Hashimoto, Y Ikeuchi, M Su'etsugu, T Suzuki, T Katayama, J Kato
MOLECULAR MICROBIOLOGY59
(
1
)
265
-
275
1 2006
-
Reconstitution of in vitro inactivation and reactivation systems of DnaA protein for the control of chromosomal replication initiation in Escherichia coli
Masayuki Su'etsugu, Kazuyuki Fujimitsu, Tsutomu Katayama
2006 IEEE INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE
334
-
+
2006
-
Protein Associations in DnaA-ATP Hydrolysis Mediated by the Replicase Clamp-Hda Complex
Peer-reviewed
Su'etsugu, M, Shimuta, T, Ishida, T, Kawakami, H, Katayama, T
J. Biol. Chem.280
6528
-
6536
8 2005
-
Molecular mechanism of DNA replication-coupled inactivation of the initiator protein in Escherichia coli: Interaction of DnaA with the sliding clamp-loaded DNA and the sliding clamp-Hda complex
Peer-reviewed
Su'etsugu, M, Takata, M, Kubota, T, Matsuda, Y, Katayama, T
Genes Cells9
509
-
522
8 2004
-
Transcriptional control for initiation of chromosomal replication in Escherichia coli: fluctuation of the level of origin transcription ensures timely initiation
Peer-reviewed
Su'etsugu, M, Emoto, A, Fujimitsu, K, Keyamura, K, Katayama, T
Genes Cells
8 2003
-
Determination of the secondary structure in solution of the Escherichia coli DnaA DNA-binding domain
Peer-reviewed
Takayuki Obita, Takafumi Iwura, Masayuki Su'etsugu, Yoichiro Yoshida, Yoshitsugu Tanaka, Tsutomu Katayama, Tadashi Ueda, Taiji Imoto
Biochemical and Biophysical Research Communications299
(
1
)
42
-
48
2002
-
DNA replication-coupled inactivation of DnaA protein in vitro: A role for DnaA arginine-334 of the AAA+ Box VIII motif in ATP hydrolysis
Peer-reviewed
Masayuki Su'Etsugu, Hironori Kawakami, Kenji Kurokawa, Toshio Kubota, Makoto Takata, Tsutomu Katayama
Molecular Microbiology40
(
2
)
376
-
386
2001