Updated on 2021/07/14

写真b

 
SASAKI Naoki
 
*Items subject to periodic update by Rikkyo University (The rest are reprinted from information registered on researchmap.)
Affiliation*
College of Science Department of Chemistry
Graduate School of Science Field of Study: Chemistry
Graduate School of Science Field of Study: Chemistry
Title*
Associate Professor
Degree
Ph.D ( The University of Tokyo )
Research Theme*
  • 分析化学、生体物質化学。細胞や組織など、生体を構成する各階層における動作原理を分子レベルで明らかにし、人類社会にとって有用な分析手法・試薬・装置の開発へとつなげることを目標としている。具体的なテーマとしては、(1)マイクロ液滴に基づく細胞内生化学プロセスの再構築、(2)マイクロ流体デバイスに基づく疑似組織構築と薬剤評価モデルへの応用など。

  • Research Interests
  • Analytical chemistry

  • Physical chemistry

  • Campus Career*
    • 4 2020 - Present 
      College of Science   Department of Chemistry   Associate Professor
    • 4 2020 - Present 
      Graduate School of Science   Field of Study: Chemistry   Associate Professor
    • 4 2020 - Present 
      Graduate School of Science   Field of Study: Chemistry   Associate Professor
    Profile

    1980年3月北海道苫小牧市に生まれる。2002年東京大学工学部応用化学科卒業,2004年同大学院工学系研究科応用化学専攻修士課程修了,2007年同博士課程修了,博士(工学)を取得 。2007年理化学研究所基礎科学特別研究員を経て,2010年日本女子大学理学部物質生物科学科助教。2013年東洋大学理工学部応用化学科講師に着任し,2016年同准教授に昇任。2020年立教大学理学部化学科准教授。2015年日本分析化学会奨励賞受賞。現在は,マイクロ流体デバイスを基盤技術とし,生命のしくみを理解して利用するバイオ分析法の開発に取り組んでいる。趣味は,男声合唱,西洋古楽(特にマドリガーレ),アイスホッケー観戦。

     

    Research Areas

    • Nanotechnology/Materials / Fundamental physical chemistry

    • Nanotechnology/Materials / Analytical chemistry

    • Life Science / Biomaterials

    • Life Science / Biomedical engineering

    • Nanotechnology/Materials / Nano/micro-systems

    Research History

    • 4 2020 - Present 
      Rikkyo University   College of Science Department of Chemistry   Associate professor

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    • 6 2021 - 9 2021 
      Ochanomizu University   Faculty of Science

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    • 4 2016 - 3 2020 
      Toyo University   Department of Applied Chemistry, Faculty of Science and Engineering   Associate professor

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    • 4 2013 - 3 2016 
      Toyo University   Department of Applied Chemistry, Faculty of Science and Engineering   Associate professor

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    • 4 2010 - 3 2013 
      Japan Women's University   Faculty of Science   Assistant professor

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    • 4 2007 - 3 2010 
      RIKEN   Special postdoctoral researcher

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    Education

    • 4 2004 - 3 2007 
      The University of Tokyo

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      Country: Japan

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    • 4 2002 - 3 2004 
      The University of Tokyo

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      Country: Japan

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    • 4 1998 - 3 2002 
      The University of Tokyo   The Faculty of Engineering   Department of Applied Chemistry

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      Country: Japan

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    Committee Memberships

    • 2020 - Present 
      MicroTAS 2020   Technical Program Committee

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    • 4 2019 - Present 
      化学とマイクロ・ナノシステム学会 会誌「化学とマイクロ・ナノシステム」   編集委員

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      Committee type:Academic society

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    • 4 2018 - Present 
      日本分析化学会   代議員

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      Committee type:Academic society

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    • 4 2016 - 3 2020 
      日本分析化学会   「分析化学」編集委員

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      Committee type:Academic society

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    • 8 2019 
      第32回バイオメディカル分析科学シンポジウム 若手シンポジウム   オーガナイザー

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      Committee type:Academic society

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    • 4 2015 - 3 2018 
      日本分析化学会 関東支部   幹事

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      Committee type:Academic society

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    • 5 2016 
      日本分析化学会   第76回分析化学討論会 討論主題 オーガナイザ 『分析化学を支える「マイクロ・ナノ」~「マイクロ・ナノ」を基盤技術とした分析化学の新潮流と将来展望』

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      Committee type:Academic society

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    Awards

    • 11 2019  
      日本化学会  第9回CSJ化学フェスタ2019 優秀ポスター発表賞  二方向観察可能な膜集積マイクロ流体デバイスを用いる角化細胞層の透過性評価
       
      杉本茉莉花、佐々木直樹

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    • 9 2019  
      日本分析化学会第68年会  学生ポスター賞  無細胞マイクロ血管モデルを用いる腫瘍細胞の血管外遊出アッセイ
       
      浅海裕一郎, 佐々木直樹

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    • 12 2016  
      Analytical Sciences  Hot article award  "A Membrane-Integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines"
       
      Naoki SASAKI, Mariko TATANOU, Tomoko SUZUKI, Yasutaka ANRAKU, Akihiro KISHIMURA, Kazunori KATAOKA, Kae SATO

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      Award type:Honored in official journal of a scientific society, scientific journal 

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    • 9 2015  
      The Japan Society for Analytical Chemistry  Award for Young Researchers  Development of micro bioanalytical devices based on deductive and constitutive approaches
       
      Naoki SASAKI

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    • 10 2012  
      第2回CSJ化学フェスタ2012  優秀ポスター発表賞  「リンパ管内皮細胞のマイクロデバイス培養と細胞間接着分子の観察」
       
      佐藤美和, 佐々木直樹, 平川聡史, 佐藤香枝

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      Country:Japan

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    • 9 2012  
      RSC Tokyo International Conference 2012  Poster Presentation Award  "Microchip-based in situ Padlock/RCA system for single DNA counting in a cell"
       
      Arisa Kuroda, Reina Ishii, Naoki Sasaki, Mats Nilsson, Kae Sato

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    • 6 2012  
      Analytical Sciences  Hot article award  ”Photochemical immobilization of cells onto a glass substrate for in situ DNA analysis”
       
      Naoki SASAKI, Anri ISU, Reina ISHII, Kae SATO

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    Papers

    • Bead-Based Padlock Rolling Circle Amplification under Molecular Crowding Conditions: The Effects of Crowder Charge and Size Peer-reviewed

      Naoki SASAKI, Chikako Kase, Kae SATO

      Analytical Sciences37 ( 5 ) 727 - 732   5 2021

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      Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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    • Fabrication of a T-Shaped Microfluidic Channel Using a Consumer Laser Cutter and Application to Monodisperse Microdroplet Formation Peer-reviewed

      Naoki Sasaki, Eisuke Sugenami

      Micromachines12 ( 2 ) 160 - 160   5 2 2021

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      Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

      The use of micrometer-sized droplets for chemical and biochemical analysis has been widely explored. Photolithography is mainly used to fabricate microfluidic devices, which is often employed to form monodisperse microdroplets. Although photolithography enables precise microfabrication, it is not readily available to biochemists because it requires specialized equipment such as clean room and mask aligners, and expensive consumables such as photoresist and silicon wafers. In this study, we fabricated a microfluidic device using a consumer laser cutter and applied it to droplet formation. Monodisperse microdroplets were formed by using an oil phase for droplet digital polymerase chain reaction (PCR) as the continuous phase and phosphate-buffered saline or polyethylene glycol solution as the dispersed phase. The droplet size decreased as the flow rate of the continuous phase increased and approached a constant value. The method developed in this study can be used to realize microdroplet-based biochemical analysis with simple devices or to construct artificial cells.

      DOI: 10.3390/mi12020160

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    • Photolithography-free Vessel-on-a-chip to Simulate Tumor Cell Extravasation Peer-reviewed

      Yuichiro Asaumi, Naoki Sasaki

      Sensors and Materials33 ( 1 ) 241 - 241   22 1 2021

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      Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MYU K.K.  

      DOI: 10.18494/sam.2021.3073

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    • Mechanistic investigation of bead-based padlock rolling circle amplification under molecular crowding conditions Peer-reviewed

      Naoki Sasaki, Chikako Kase, Masaki Chou, Genki Nakazato, Kae Sato

      Analytical Biochemistry593   113596 - 113596   3 2020

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

      DOI: 10.1016/j.ab.2020.113596

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    • Photoactivatable hydrogel interfaces for resolving the interplay of chemical, mechanical, and geometrical regulation of collective cell migration Peer-reviewed

      Shota Yamamoto, Kei Okada, Naoki Sasaki, Alice Chinghsuan Chang, Kazuo Yamaguchi, Jun Nakanishi

      Langmuir35 ( 23 ) 7459 - 7468   6 2019

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      DOI: 10.1021/acs.langmuir.8b02371

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    • Pancreatic stellate cells derived from human pancreatic cancer demonstrate aberrant SPARC-dependent ECM remodeling in 3D engineered fibrotic tissue of clinically relevant thickness. Peer-reviewed International journal

      Hiroyoshi Y Tanaka, Kentaro Kitahara, Naoki Sasaki, Natsumi Nakao, Kae Sato, Hirokazu Narita, Hiroshi Shimoda, Michiya Matsusaki, Hiroshi Nishihara, Atsushi Masamune, Mitsunobu R Kano

      Biomaterials192   355 - 367   2 2019

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      Language:English   Publishing type:Research paper (scientific journal)  

      Desmoplasia is a hallmark of pancreatic cancer and consists of fibrotic cells and secreted extracellular matrix (ECM) components. Various in vitro three-dimensional (3D) models of desmoplasia have been reported, but little is known about the relevant thickness of the engineered fibrotic tissue. We thus measured the thickness of fibrotic tissue in human pancreatic cancer, as defined by the distance from the blood vessel wall to tumor cells. We then generated a 3D fibrosis model with a thickness reaching the clinically observed range using pancreatic stellate cells (PSCs), the main cellular constituent of pancreatic cancer desmoplasia. Using this model, we found that Collagen fiber deposition was increased and Fibronectin fibril orientation drastically remodeled by PSCs, but not normal fibroblasts, in a manner dependent on Transforming Growth Factor (TGF)-β/Rho-Associated Kinase (ROCK) signaling and Matrix Metalloproteinase (MMP) activity. Finally, by targeting Secreted Protein, Acidic and Rich in Cysteine (SPARC) by siRNA, we found that SPARC expression in PSCs was necessary for ECM remodeling. Taken together, we developed a 3D fibrosis model of pancreatic cancer with a clinically relevant thickness and observed aberrant SPARC-dependent ECM remodeling in cancer-derived PSCs.

      DOI: 10.1016/j.biomaterials.2018.11.023

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    • Photolithography-free Skin-on-a-chip for Parallel Permeation Assays Peer-reviewed

      Naoki Sasaki, Kimiaki Tsuchiya, Hironori Kobayashi

      Sensors and Materials31 ( 1 ) 107 - 115   1 2019

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      DOI: 10.18494/SAM.2019.2125

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    • Label-free rapid separation and enrichment of bone marrow-derived mesenchymal stem cells from a heterogeneous cell mixture using a dielectrophoresis device Peer-reviewed

      Junya Yoshioka, Yu Ohsugi, Toru Yoshitomi, Tomoyuki Yasukawa, Naoki Sasaki, Keitaro Yoshimoto

      Sensors (Switzerland)18 ( 9 ) 3007   8 9 2018

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      Language:English   Publishing type:Research paper (scientific journal)  

      © 2018 by the authors. Licensee MDPI, Basel, Switzerland. Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophoresis (DEP) device comprising saw-shaped electrodes. Upon application of an electric field, HL-60 cells as models of promyelocytes aggregated and floated between the saw-shaped electrodes, while UE7T-13 cells as models of BMSCs were effectively captured on the tips of the saw-shaped electrodes. After washing out the HL-60 cells from the device selectively, the purity of the UE7T-13 cells was increased from 33% to 83.5% within 5 min. Although further experiments and optimization are required, these results show the potential of the DEP device as a label-free rapid cell isolation system yielding high purity for rare and precious cells such as BMSCs.

      DOI: 10.3390/s18093007

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    • Fabrication of Microfluidic Cell Culture Devices Using a Consumer Laser Cutter Peer-reviewed

      Naoki SASAKI, Tomomi HAYASHI, Nanako INOUE, Masahiro ONISHI

      BUNSEKI KAGAKU67 ( 7 ) 379 - 386   7 2018

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      Language:Japanese   Publishing type:Research paper (scientific journal)  

      DOI: 10.2116/bunsekikagaku.67.379

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    • Molecular crowding improves bead-based padlock rolling circle amplification Peer-reviewed

      Naoki Sasaki, Yoshitaka Gunji, Chikako Kase, Kae Sato

      ANALYTICAL BIOCHEMISTRY519   15 - 18   2 2017

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      Bead-based padlock rolling circle amplification (RCA), an ultrasensitive and accurate DNA detection technique, was conducted in a molecular crowding environment created by poly(ethylene glycol) (PEG). The number of RCA products generated increased and exhibited a bell-shaped dependence on PEG concentration. Experiments using magnetic beads suggested that facilitation of DNA ligation and hybridization is the main reason for the observed increase. Selectivity of the technique was retained in the presence of PEG. This technique is simple and can be utilized to detect target DNA with high accuracy and sensitivity in a variety of areas such as medical diagnosis and food analysis. (C) 2016 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.ab.2016.12.002

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    • A Membrane-integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines Peer-reviewed

      Naoki Sasaki, Mariko Tatanou, Tomoko Suzuki, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, Kae Sato

      ANALYTICAL SCIENCES32 ( 12 ) 1307 - 1314   12 2016

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

      Nanoparticles have been widely utilized to deliver drugs from blood vessels to target tissues. A crucial issue concerning nanoparticle-based drug delivery is to discuss the relationship between experimentally-obtained permeability and physical parameters. Although nanoparticles can permeate vascular pores, because the size and shape of the pores are essentially non-uniform, conventional animal testing and recent cell-based microfluidic devices are unable to precisely evaluate the effects of physical parameters (e.g. pore size and nanoparticle size) on permeation. In this study, we present a membrane integrated microfluidic device to study permeation of nanoparticles through straight micropores. Porous membranes possessing uniform straight pores were utilized. The effects of pore size and pressure difference across the pores on nanoparticle permeation were examined. The experimentally determined permeability coefficient of 1.0 mu m-pore membrane against 100 nm-diameter nanoparticles agreed well with the theoretical value obtained for convectional permeation. Our method can be utilized to clarify the relationship between the experimentally-obtained permeability and physical parameters, and will help rational design of nanomedicines.

      DOI: 10.2116/analsci.32.1307

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    • Alternating Current Cloud Point Extraction on a Microfluidic Chip: the Use of Ferrocenyl Surfactants Peer-reviewed

      Yuya Usui, Naoki Sasaki

      ANALYTICAL SCIENCES32 ( 1 ) 109 - 111   1 2016

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

      Alternating current cloud point extraction (ACPE) is a preconcentration technique that can be employed in the analysis of membrane proteins on a microfluidic chip. However, the selectivity of ACPE relies on the hydrophobicity of the analytes. In this study, 11-ferrocenyltrimethylundecylammonium bromide (FTMA) was utilized to introduce electrostatic interaction as part of the ACPE technique. The use of ACPE with oxidized FTMA resulted in efficient concentration of fluorescently labeled anionic membrane proteins. We expect the approach outlined in this report to be useful in the preconcentration technique of microchip electrophoresis.

      DOI: 10.2116/analsci.32.109

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    • Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone Peer-reviewed

      Kiichi Sato, Sayaka Kikuchi, Eri Yoshida, Reina Ishh, Naoki Sasaki, Kin-ichi Tsunoda, Kae Sato

      ANALYTICAL SCIENCES32 ( 1 ) 113 - 116   1 2016

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

      The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.

      DOI: 10.2116/analsci.32.113

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    • Analytical applications of microfluidic vascular models Peer-reviewed

      Naoki Sasaki, Kae Sato

      Bunseki Kagaku65 ( 5 ) 241 - 247   2016

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      Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Analytical Chemistry  

      Microfluidic vascular models were utilized as a novel platform of biochemical analysis. Cell-based vascular models were developed by culturing vascular cells in microchannels. A palmtop-sized microfluidic cell culture system, consisting of a microfluidic device and a miniaturized infusion pump, was developed. The proliferation speed and protein expression of human endothelial cells cultured in the system were the same as cells in a culture flask. A microfluidic model of microcirculation containing both blood and lymphatic vessels was developed to examine the vascular permeability. The model was applied to the assay of habu snake venom, and the assay time was reduced from 24 h to 30 min. On the other hand, cell-free microfluidic vascular models were developed in order to precisely understand the mechanism of microcirculation. The permeation of nanoparticles through artificial vascular walls was evaluated. The permeation of nanoparticles through interstitium surrounding the blood vessels was also evaluated under cell-free conditions. These models can bridge in vivo analysis and in vitro analysis.

      DOI: 10.2116/bunsekikagaku.65.241

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    • Microcirculation-on-a-Chip: A Microfluidic Platform for Assaying Blood- and Lymphatic-Vessel Permeability Peer-reviewed

      Miwa Sato, Naoki Sasaki, Manabu Ato, Satoshi Hirakawa, Kiichi Sato, Kae Sato

      PLOS ONE10 ( 9 ) e0137301   9 2015

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

      We developed a microfluidic model of microcirculation containing both blood and lymphatic vessels for examining vascular permeability. The designed microfluidic device harbors upper and lower channels that are partly aligned and are separated by a porous membrane, and on this membrane, blood vascular endothelial cells (BECs) and lymphatic endothelial cells (LECs) were cocultured back-to-back. At cell-cell junctions of both BECs and LECs, claudin-5 and VE-cadherin were detected. The permeability coefficient measured here was lower than the value reported for isolated mammalian venules. Moreover, our results showed that the flow culture established in the device promoted the formation of endothelial cell-cell junctions, and that treatment with histamine, an inflammation-promoting substance, induced changes in the localization of tight and adherens junction-associated proteins and an increase in vascular permeability in the microdevice. These findings indicated that both BECs and LECs appeared to retain their functions in the microfluidic coculture platform. Using this microcirculation device, the vascular damage induced by habu snake venom was successfully assayed, and the assay time was reduced from 24 h to 30 min. This is the first report of a microcirculation model in which BECs and LECs were cocultured. Because the micromodel includes lymphatic vessels in addition to blood vessels, the model can be used to evaluate both vascular permeability and lymphatic return rate.

      DOI: 10.1371/journal.pone.0137301

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    • Alternating current cloud point extraction on a microchip: The effect of electrode geometry Peer-reviewed

      Naoki Sasaki, Chisaki Maekawa, Kae Sato

      ELECTROPHORESIS36 ( 3 ) 424 - 427   2 2015

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

      We report on the effect of electrode geometry on alternating current cloud point extraction (ACPE). ACPE is a technique utilized to extract membrane-associated biomolecules in an electrode-integrated microfluidic channel. In this study, we investigated the effect of gap size (4 approximate to 22 m) between microband electrodes on ACPE. A decrease in gap size resulted in efficient and rapid concentration of fluorescent-labeled phospholipids, a model of membrane-associated biomolecules. We also investigated the effect of applied voltage amplitude on ACPE using devices with decreased electrode gap size. When the gap was small, ACPE was achieved with low applied voltages. ACPE of membrane proteins extracted from HeLa cells was also studied to demonstrate the applicability of the ACPE to real samples. The results provide a guideline to improve the performance of ACPE and facilitate application of the ACPE technique as part of an overall analytical process.

      DOI: 10.1002/elps.201400410

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    • AC electrokinetics for bioanalysis on a microchip Peer-reviewed

      Naoki Sasaki

      Bunseki Kagaku64 ( 1 ) 1 - 8   2015

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      Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Analytical Chemistry  

      AC electrokinetics is concerned with an induced motion of particles and fluids under nonuniform AC electric fields in microscale. The physicochemical nature of this phenomena, which cannot be observed in macroscale, is of great interest. In addition, AC electokinetics can be easily induced by the use of microelectrodes, which can be easily integrated into microchannels. In this paper, applications are described of AC electrokinetics as unit operations in bioanalysis on a microchip. AC electrokinetic fluid flow was utilized to mix fluids in a short time. The principle of cloud point extraction was combined with AC electrokinetics to concentrate membrane-associated biomolecules in a microchannel. These operations will contribute to the functionalization of microchip for bioanalysis.

      DOI: 10.2116/bunsekikagaku.64.1

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    • Magnetic resonance imaging of a microvascular-interstitium model on a microfluidic device Peer-reviewed

      Naoki Sasaki, Jun-Ichiro Jo, Ichio Aoki, Kae Sato

      ANALYTICAL BIOCHEMISTRY458   72 - 74   8 2014

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      We report a microvascular-interstitium model on microfluidic devices to study leakage of drugs from blood vessels under in vivo-like flow conditions. We employed magnetic resonance imaging to demonstrate the compatibility of the model for experimental animals and humans. We observed transport of two types of different molecular-weight contrast agents into the model interstitium. The ratio of the transport rates of agents agreed with the ratio calculated from diffusion coefficients of the agents. We expect that the model will be useful for the estimation and evaluation of leakage of many kinds of agents in vivo. (C) 2014 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.ab.2014.03.020

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    • Microfluidics for nano-pathophysiology

      Kae Sato, Naoki Sasaki, Helene Andersson Svahn, Kiichi Sato

      ADVANCED DRUG DELIVERY REVIEWS74   115 - 121   7 2014

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      Language:English   Publisher:ELSEVIER SCIENCE BV  

      Nanotechnology-based drug delivery systems hold promise for innovative medical treatment of cancers. While drug materials are constantly under development, there are no practical cell-based models to assess whether these materials can reach the target tissue. Recently developed microfluidic systems have revolutionized cell-based experiments. In these systems, vascular endothelial cells and interstitium are set in microchannels that mimic microvessels. Drug permeability can be assayed in these blood vessel models under fluidic conditions that mimic blood flow. In this review, we describe device fabrication, disease model development, nanoparticle permeability assays, and the potential utility of these systems in the future. (C) 2013 Elsevier B.V. All rights reserved.

      DOI: 10.1016/j.addr.2013.08.009

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    • Bead-based padlock rolling circle amplification for single DNA molecule counting Peer-reviewed

      Kae Sato, Reina Ishii, Naoki Sasaki, Kiichi Sato, Mats Nilsson

      ANALYTICAL BIOCHEMISTRY437 ( 1 ) 43 - 45   6 2013

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination. (C) 2013 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.ab.2013.02.016

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    • Alternating current cloud point extraction on a microchip: A comprehensive study Peer-reviewed

      Naoki Sasaki, Azusa Takemura, Kae Sato

      ELECTROPHORESIS33 ( 21 ) 3159 - 3165   11 2012

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

      We present a comprehensive study of alternating current cloud point extraction (ACPE) on a microchip. ACPE is an extraction technique for preconcentration of membrane-associated biomolecules. To characterize and optimize ACPE, we carried out ACPE experiments under various experimental conditions including amplitude and frequency of applied voltages, flow velocity, and concentration of surfactant, analyte, and salt. We found that ACPE has an amplitude threshold (15 V-pp), above which the extraction was more efficient. The dependence of the extraction on frequency (>5 MHz) was insignificant. Efficient extraction was achieved when the velocity of the test solution was 0.10 similar to 0.67 mm s(-1) and the concentration of surfactant was 0.10 similar to 1.0%. In contrast, the extraction was independent of the concentration of analytes (0.20 similar to 20 mu mol dm(-3)). The technique was applicable to solutions with a salt concentration of 0.050 similar to 0.15 mol dm(-3) under temperature control of the devices. Solution temperature in ACPE was also studied. These results provide guidelines for use of the ACPE technique in microfluidic chemical and biochemical analyses.

      DOI: 10.1002/elps.201200229

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    • Fluid mixing using AC electrothermal flow on meandering electrodes in a microchannel Peer-reviewed

      Naoki Sasaki, Takehiko Kitamori, Haeng-Boo Kim

      ELECTROPHORESIS33 ( 17 ) 2668 - 2673   9 2012

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

      The mixing of fluids using AC electrothermal flow (AC-ETF) is presented. A pair of coplanar electrodes with a sinusoidal interelectrode gap was used to enhance the mixing in a microchannel. To demonstrate the performance of the mixer, conventional dilution experiments were conducted using Texas Red-labeled dextran. The dependence of mixing on the salt concentration (10-3 similar to 10-1 mol dm-3) of the solutions and frequency (100 kHz similar to 5 MHz) of the applied voltage were investigated. AC-ETF was responsible for the mixing at salt concentrations >10-2 mol dm-3, whereas the effect of AC-EOF was suggested to play a role at concentrations <10-2 mol dm-3 in the low-frequency region. The fluorogenic reaction of human serum albumin (HSA) with SYPRO Red in the mixer was also examined, and results showed that enrichment of fluorescence intensity and an almost uniform distribution of stained HSA were achieved. The present mixer can be employed as a powerful tool to facilitate efficient chemical and biomedical analysis on microfluidic devices.

      DOI: 10.1002/elps.201200099

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    • A palmtop-sized microfluidic cell culture system driven by a miniaturized infusion pump Peer-reviewed

      Naoki Sasaki, Mika Shinjo, Satoshi Hirakawa, Masahiro Nishinaka, Yo Tanaka, Kazuma Mawatari, Takehiko Kitamori, Kae Sato

      ELECTROPHORESIS33 ( 12 ) 1729 - 1735   7 2012

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      A palmtop-sized microfluidic cell culture system is presented. The system consists of a microfluidic device and a miniaturized infusion pump that possesses a reservoir of culture medium, an electrical control circuit, and an internal battery. The footprint of the system was downsized to 87 x 57 mm, which is, to the best of our knowledge, the smallest integrated cell culture system. Immortalized human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC) were cultured in the system. HMEC-1 in the system proliferated at the same speed as cells in a microchannel perfused by a syringe pump and cells in a culture flask. HUVEC in the system oriented along the direction of the fluid flow. Claudin-5, a tight junction protein, was localized along the peripheries of the HUVEC. We expect that the present system is applicable to various cell types as a stand-alone and easy-to-use system for microfluidic bioanalysis.

      DOI: 10.1002/elps.201100691

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    • Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis Peer-reviewed

      Naoki Sasaki, Anri Isu, Reina Ishii, Kae Sato

      ANALYTICAL SCIENCES28 ( 6 ) 537 - 539   6 2012

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      A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.

      DOI: 10.2116/analsci.28.537

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    • Hydrodynamic Cell Pairing and Cell Fusion through a Microslit on a Microfluidic Device Peer-reviewed

      Naoki Sasaki, Jiansheng Gong, Makoto Sakuragi, Kazuo Hosokawa, Mizuo Maeda, Yoshihiro Ito

      JAPANESE JOURNAL OF APPLIED PHYSICS51 ( 3 ) 030206   3 2012

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      Positioning of two different types of cells in contact with each other is of particular importance to analyze interactions between the cells. However, previous methods require sequential injection of two different cell suspensions and flow switching during the operation. Here, we present a novel method to pair two different types of cells on microfluidic devices. Single-step pairing was achieved by introducing each cell suspension from different inlets into the microchannel which has a microslit arranged with a hydrodynamic weir. As an application of the pairing, cell fusion through the microslit was studied. (C) 2012 The Japan Society of Applied Physics

      DOI: 10.1143/JJAP.51.030206

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    • Recent Applications of AC Electrokinetics in Biomolecular Analysis on Microfluidic Devices Peer-reviewed

      Naoki Sasaki

      ANALYTICAL SCIENCES28 ( 1 ) 3 - 8   1 2012

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      AC electrokinetics is a generic term that refers to an induced motion of particles and fluids under nonuniform AC electric fields. The AC electric fields are formed by application of AC voltages to microelectrodes, which can be easily integrated into tnicrofluidic devices by standard microfabrication techniques. Moreover, the magnitude of the motion is large enough to control the mass transfer on the devices. These advantages are attractive for biomolecular analysis on the microfluidic devices, in which the characteristics of small space and microfluidics have been mainly employed. In this review, I describe recent applications of AC electrokinetics in biomolecular analysis on microfluidic devices. The applications include fluid pumping and mixing by AC electrokinetic flow, and manipulation of biomolecules such as DNA and proteins by various AC electrokinetic techniques. Future prospects for highly functional biomolecular analysis on microfluidic devices with the aid of AC electrokinetics are also discussed.

      DOI: 10.2116/analsci.28.3

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    • Experimental and Theoretical Characterization of an AC Electroosmotic Micromixer Peer-reviewed

      Naoki Sasaki, Takehiko Kitamori, Haeng-Boo Kim

      ANALYTICAL SCIENCES26 ( 7 ) 815 - 819   7 2010

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

      We have reported on a novel microfluidic mixer based on AC electroosmosis. To elucidate the mixer characteristics, we performed detailed measurements of mixing under various experimental conditions including applied voltage, frequency and solution viscosity. The results are discussed through comparison with results obtained from a theoretical model of AC electroosmosis. As predicted from the theoretical model, we found that a larger voltage (similar to 20 V(p.p)) led to more rapid mixing, while the dependence of the mixing on frequency (1 - 5 kHz) was insignificant under the present experimental conditions. Furthermore, the dependence of the mixing on viscosity was successfully explained by the theoretical model, and the applicability of the mixer in viscous solution (2.83 mPa s) was confirmed experimentally. By using these results, it is possible to estimate the mixing performance under given conditions. These estimations can provide guidelines for using the mixer in microfluidic chemical analysis.

      DOI: 10.2116/analsci.26.815

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    • Artificial chaperone-assisted refolding in a microchannel Peer-reviewed

      Etsushi Yamamoto, Satoshi Yamaguchi, Naoki Sasaki, Haeng-Boo Kim, Takehiko Kitamori, Teruyuki Nagamune

      BIOPROCESS AND BIOSYSTEMS ENGINEERING33 ( 1 ) 171 - 177   1 2010

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

      Protein refolding using a simple dilution method in a microchannel often led to the formation of protein aggregates, which bound to the microchannel wall, resulting in low refolding yields. To inhibit aggregation and improve refolding yields, an artificial chaperone-assisted (ACA) refolding, which employed detergents and beta-cyclodextrin was used. Model proteins, hen egg white lysozyme and yeast alpha-glucosidase, were successfully refolded in a microchannel. The microscopic observation showed that the ACA method suppressed protein aggregation and facilitated the refolding of lysozyme, whereas significant aggregation was observed when a simple dilution method was employed. The ACA method increased the lysozyme refolding yield by 40% over the simple dilution approach. Similarly, for alpha-glucosidase, the refolding yield using the ACA method (ca. 50%) was approximately three times compared with the simple dilution method. The ACA refolding method is a suitable approach to use in the refolding of proteins using a microfluidic system.

      DOI: 10.1007/s00449-009-0374-1

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    • Alternating current cloud point extraction on a microchip for preconcentration of membrane-associated biomolecules Peer-reviewed

      Naoki Sasaki, Kazuo Hosokawa, Mizuo Maeda

      LAB ON A CHIP9 ( 9 ) 1168 - 1170   2009

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

      Joule heating and negative dielectrophoresis, caused by AC electric fields in a microchannel, have been employed for cloud point extraction of phospholipid as a model of membrane-associated biomolecules.

      DOI: 10.1039/b901007f

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    • AC electroosmotic micromixer for chemical processing in a microchannel Peer-reviewed

      Naoki Sasaki, Takehiko Kitamori, Haeng-Boo Kim

      Lab on a Chip6 ( 4 ) 550 - 554   2006

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      Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

      DOI: 10.1039/b515852d

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    • Spectroelectrochemical detection using thermal lens microscopy with a glass-substrate microelectrode-microchannel chip Peer-reviewed

      Haeng-Boo Kim, Tomokazu Hagino, Naoki Sasaki, Noriyuki Watanabe, Takehiko Kitamori

      Journal of Electroanalytical Chemistry577 ( 1 ) 47 - 53   3 2005

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      DOI: 10.1016/j.jelechem.2004.11.012

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    Misc.

    • Skin-on-a-chipの研究動向 Invited Peer-reviewed

      杉本茉莉花, 佐々木直樹

      化学とマイクロ・ナノシステム18 ( 2 )   10 2019

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    • 粒ぞろいの細胞もどきを作る Invited Peer-reviewed

      佐々木 直樹

      ぶんせき ( 6 ) 215 - 215   6 2016

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    • 光誘起動電現象で生体分子を濃縮する

      佐々木直樹

      ぶんせき ( 7 ) 361   2010

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    Presentations

    • 多孔膜垂直配置マイクロ流体デバイス

      佐々木直樹

      令和2年度分析イノベーション交流会  26 2 2021 

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      Event date: 25 2 2021 - 26 2 2021

      Language:Japanese   Presentation type:Oral presentation (general)  

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    • Simulation of tumor cell extravasation on a photolithography-free microfluidic device

      Yuichiro Asaumi, Naoki Sasaki

      The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020)  6 10 2020 

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      Event date: 5 10 2020 - 9 10 2020

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    • 二方向観察可能なskin-on-a-chipによる抗炎症剤評価(2):トリコテセン類への応用

      杉本茉莉花、安藤直子、佐々木直樹

      日本化学会第100春季年会  23 3 2020 

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      Event date: 22 3 2020 - 25 3 2020

      Language:Japanese   Presentation type:Oral presentation (general)  

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    • 二方向観察可能なskin-on-a-chip による抗炎症剤評価

      杉本茉莉花、佐々木直樹

      化学とマイクロ・ナノシステム学会第40回研究会  19 11 2019 

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      Event date: 19 11 2019 - 21 11 2019

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    • 腫瘍細胞の血管外遊出を評価するマイクロデバイスの開発

      浅海裕一郎、佐々木直樹

      化学とマイクロ・ナノシステム学会第40回研究会  19 11 2019 

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      Event date: 19 11 2019 - 21 11 2019

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    • A TWO-WAY MEMBRANE-INTEGRATED MICROFLUIDIC DEVICE FOR PERMEATION ASSAYS International conference

      Marika Sugimoto, Keisuke Yanagisawa, Naoki Sasaki

      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  27 10 2019 

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    • EXTRAVASATION OF SOFT NANOPARTICLES SIMULATED ON AN EASY-TO-OBSERVE MEMBRANE-INTEGRATED MICROFLUIDIC DEVICE International conference

      Mayu Watanabe, Yumi Moriya, Hiroaki Matsuba, Akihiro Kishimura, Yoshiki Katayama, Naoki Sasaki

      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  27 10 2019 

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    • 二方向観察可能な膜集積マイクロ流体デバイスを用いる角化細胞層の透過性評価

      杉本茉莉花, 佐々木直樹

      第9回CSJ化学フェスタ2019  15 10 2019 

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    • Tumor cell extravasation assay on a photolithography-free microfluidic device International conference

      Yuichiro Asaumi, Naoki Sasaki

      Lab-on-a-Chip & Microfluidics World Congress 2019  7 10 2019 

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    • 二方向観察可能なマイクロ流体デバイスを用いる細胞層の透過性評価

      杉本茉莉花, 佐々木直樹

      日本分析化学会第68年会  11 9 2019 

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    • 無細胞マイクロ血管モデルを用いる腫瘍細胞の血管外遊出アッセイ

      浅海裕一郎, 佐々木直樹

      日本分析化学会第68年会  11 9 2019 

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    • A cell-free vascular model for tumour cell extravasation assay International conference

      Yuichiro Asaumi, Naoki Sasaki

      RSC Tokyo International Conference 2019  4 9 2019 

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    • Evaluation of permeability of keratinocyte layers using a two-way membrane-integrated microfluidic device International conference

      Marika Sugimoto, Keisuke Yanagisawa, Naoki Sasaki

      RSC Tokyo International Conference 2019  4 9 2019 

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    • Organ-on-a-chipによる臓器機能再現 Invited

      佐々木直樹

      第32回バイオメディカル分析科学シンポジウム  24 8 2019 

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    • Organ-on-a-chip による疑似臓器構築 Invited

      佐々木直樹

      第32回日本Archaea研究会講演会  20 7 2019 

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    • Photolithography-free microfluidic device to study tumor cell extravasation

      Yuichiro Asaumi, Naoki Sasaki

      The 99th CSJ Annual Meeting  16 3 2019 

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    • A dual-membrane microfluidic device for chemotaxis assays of immune cells International conference

      Marika Sugimoto, Naoki Sasaki

      3rd International Symposium on Nanoarchitectonics for Mechanobiology  7 3 2019 

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    • Photolithography-free microfluidic device to evaluate tumor cell migration International conference

      Yuichiro Asaumi, Naoki Sasaki

      3rd International Symposium on Nanoarchitectonics for Mechanobiology  7 3 2019 

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    • 無細胞マイクロ腫瘍血管モデルを用いるナノ粒子の血管漏出性シミュレーション

      守谷侑美, 佐々木直樹

      第28回日本MRS年次大会  18 12 2018 

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    • A DUAL-MEMBRANE MICROFLUIDIC DEVICE FOR CELL MIGRATION ASSAY International conference

      Marika SUGIMOTO, Fuka NAGATOMI, Naoki SASAKI

      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2018)  11 11 2018 

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    • A MEMBRANE-INTEGRATED MICROFLUIDIC DEVICE FOR SIMULATING NANOPARTICLE EXTRAVASATION IN TUMOR MICROENVIRONMENT International conference

      Yumi Moriya, Naoki Sasaki

      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2018)  11 11 2018 

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    • PHOTOLITHOGRAPHY-FREE TUMOR-ON-A-CHIP TO STUDY NANOPARTICLE EXTRAVASATION International conference

      Yuki Ichikawa, Tomomi Hayashi, Naoki Sasaki

      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2018)  11 11 2018 

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    • 並行多孔膜組み込みマイクロデバイスによる細胞遊走アッセイ

      杉本茉莉花, 永富風花, 佐々木直樹

      日本分析化学会第67年会  12 9 2018 

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    • 無細胞マイクロ血管モデルを用いるナノ粒子の透過性評価

      守谷侑美, 紺野仁美, 佐々木直樹

      日本分析化学会第67年会  12 9 2018 

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    • 多孔膜垂直配置マイクロ流体デバイスを用いるバイオ分析 Invited

      佐々木直樹

      バイオメディカルエンジニアリング 新技術説明会  11 9 2018 

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    • 疑似細胞空間・疑似組織を基盤とするバイオ分析 Invited

      佐々木直樹

      首都圏産業活性化協会 第31回医療イノベーションフォーラム  1 6 2018 

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    • 誰でも作れるマイクロ流体デバイス Invited

      佐々木直樹

      彩の国ビジネスアリーナ2018 技術シーズ発表会  24 1 2018 

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    • ナノ薬剤開発のための無細胞マイクロ血管モデル Invited

      佐々木直樹

      第27回日本MRS年次大会  5 12 2017 

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    • Simulation of nanoparticle extravasation using an easy-to-observe membrane-intgrated microfluidic device International conference

      Yumi MORIYA, Miki ODANAKA, Naoki SASAKI

      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)  22 10 2017 

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    • マイクロビーズPadlock/RCA法における高分子の影響

      加瀬央子, 佐々木直樹, 佐藤香枝

      第7回CSJ化学フェスタ2017  17 10 2017 

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    • マイクロ流体デバイスと疑似血液を用いるナノ粒子の血管透過性評価

      守谷侑美, 小田中美希, 佐々木直樹

      第7回CSJ化学フェスタ2017  17 10 2017 

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    • 無細胞血管-間質マイクロモデルを用いるナノ粒子の透過性評価

      守谷侑美, 小田中美希, 佐々木直樹

      化学とマイクロ・ナノシステム学会第36回研究会  4 10 2017 

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    • マイクロ流体デバイスとヒト全血を用いたナノ粒子の血管透過性評価

      守谷侑美, 小田中美希, 佐々木直樹

      日本分析化学会第66年会  9 9 2017 

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    • 細胞集団移動の基質依存性の分析に用いる表面化学・力学特性が制御された光応答基板の開発

      岡田佳, 山本翔太, 山口和夫, 佐々木直樹, 中西淳

      日本分析化学会第66年会  9 9 2017 

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    • 鋸型電極デバイスの誘電泳動を利用するヒト骨髄由来細胞の非標識な分離と濃縮

      吉岡純矢, 大杉悠, 吉冨徹, 安川智之, 佐々木直樹, 吉本敬太郎

      日本分析化学会第66年会  9 9 2017 

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    • A membrane-integrated microfluidic device for co-culture analysis International conference

      Tomomi HAYASHI, Nanako INOUE, Naoki. SASAKI

      RSC Tokyo International Conference 2017  7 9 2017 

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    • Effects of macromolecular crowding on bead-based padlock rolling circle amplification International conference

      Chikako KASE, Naoki. SASAKI, Kae. SATO

      RSC Tokyo International Conference 2017  7 9 2017 

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    • Evaluation of vascular permeation of nanoparticles by using a membrane-integrated microfluidic device International conference

      Yumi MORIYA, Miki ODANAKA, Naoki SASAKI

      RSC Tokyo International Conference 2017  7 9 2017 

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    • 生体にまねぶバイオ分析 Invited

      佐々木直樹

      ナノ茶論  19 5 2017 

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    • Organ on a Chipによる薬剤評価 Invited

      佐々木直樹

      第3回 産学医連携シーズマッチングwith東洋大学  16 3 2017 

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    • FABRICATION OF AN EASY-TO-OBSERVE MEMBRANE-INTEGRATED MICROFLUIDIC DEVICE International conference

      Satoshi Watanabe, Naoki Sasaki

      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2016)  12 10 2016 

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    • 表面化学・力学特性が制御された光応答基板を用いる細胞集団移動の分析

      岡田佳, 山本翔太, 佐々木直樹, 山口和夫, 中西淳

      日本分析化学会第65年会  14 9 2016 

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    • ドライエッチングによる石英ガラスの深堀マイクロ流路加工の検討

      松下和樹, 森川響二朗, 佐々木直樹, 塚原剛彦

      化学とマイクロ・ナノシステム学会第34回研究会  6 9 2016 

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    • Development of photoactivatable substrate with tuned surface chemistry and substrate stiffness as a platform for collective cell migration study International conference

      Kei Okada, Shota Yamamoto, Naoki Sasaki, Kazuo Yamaguchi, Jun Nakanishi

      2nd International symposium on nanoarchitectonics for mechanobiology  27 7 2016 

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    • 無細胞マイクロ微小循環モデルの開発

      臼井裕也, 佐々木直樹

      第76回分析化学討論会  28 5 2016 

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    • 腫瘍への届きやすさ評価 東洋大、マイクロチップ開発

      日経産業新聞

      2 5 2016 

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    • オンチップ抽出クロマトグラフィーシステムの構築とランタノイド分析

      松下和樹, 森川響二朗, 佐々木直樹, 塚原剛彦

      化学とマイクロ・ナノシステム学会第33回研究会  25 4 2016 

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    • マイクロビーズPadlock/RCA 法におけるPolyethylene glycol(PEG)の影響

      加瀬央子, 佐々木直樹, 佐藤香枝

      化学とマイクロ・ナノシステム学会第33回研究会  25 4 2016 

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    • マイクロビーズPadlock/RCA 法における各種分子クラウディング剤の影響

      中畑友祐, 佐藤香枝, 佐々木直樹

      化学とマイクロ・ナノシステム学会第33回研究会  25 4 2016 

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      Venue:東京大学 生産技術研究所  

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    • 多孔膜集積マイクロ流体デバイスによるナノ粒子の透過性分析

      土屋公彰, 佐々木直樹

      日本化学会第96春季年会  24 3 2016 

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    • 腫瘍血管‐間質複合型無細胞マイクロ流体デバイスの開発

      臼井裕也, 渡邉学志, 佐々木直樹

      日本化学会第96春季年会  24 3 2016 

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    • オンチップ抽出クロマトグラフィーによる金属イオン分析システムの開発

      松下和樹, 森川響二朗, 佐々木直樹, 塚原剛彦

      第1回 次世代イニシアティブ廃炉技術カンファレンス  16 3 2016 

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    • Molecular crowding-assisted on-bead rolling circle amplification for single DNA molecule counting International conference

      Naoki Sasaki, Yoshitaka Gunji, Kae Sato

      Pacifichem 2015  12 2015 

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    • DNAバルジ構造を利用した5-メチルシトシンの電気化学分析

      吉田岳史, 佐々木直樹, 栁澤博幸, 栗田僚二, 丹羽 修

      第5回CSJ化学フェスタ2015  10 2015 

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    • 演繹的及び構成的アプローチに基づくマイクロバイオ分析デバイスの開発 Invited

      佐々木直樹

      日本分析化学会第64年会  9 2015 

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    • Alternating current cloud point extraction: the use of ferrocenyl surfactant International conference

      Yuya Usui, Naoki Sasaki

      RSC Tokyo International Conference 2015  9 2015 

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    • Cell-free microfluidic vascular models for nanoDDS Invited

      Naoki SASAKI

      2nd Asian Symposium for Analytical Sciences (in the 64th Annual Meeting of The Japan Society for Analytical Chemistry)  9 2015 

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    • Single DNA molecules detection by on-bead rolling circle amplification in a crowded environment International conference

      Yoshitaka Gunji, Akiho Numata, Kae Sato, Naoki Sasaki

      RSC Tokyo International Conference 2015  9 2015 

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    • DNAバルジ構造を利用した5-メチルシトシンの電気化学分析

      吉田岳史, 佐々木直樹, 栁澤博幸, 栗田僚二, 丹羽修

      第75回分析化学討論会  5 2015 

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    • 分子クラウディングを利用したマイクロビーズPadlock/RCA法の高感度化

      郡司良隆, 佐藤香枝, 佐々木直樹

      第75回分析化学討論会  5 2015 

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    • Molecular crowding improves single DNA molecules detection by on-bead rolling circle amplification International conference

      Yoshitaka Gunji, Kae Sato, Naoki Sasaki

      RSC Tokyo International Conference 2014  9 2014 

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    • マイクロチップ交流曇点抽出法の水性二相系への展開

      佐々木直樹, 安達尭史

      化学とマイクロ・ナノシステム学会第29回研究会  5 2014 

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    • 分子クラウディングを利用したマイクロビーズPadlock/RCA法の高機能化

      青山春樹, 佐藤香枝, 佐々木直樹

      第74回分析化学討論会  5 2014 

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    • 分子クラウディング環境下でのマイクロビーズPadlock/RCA法による単一DNA検出

      青山春樹, 佐藤香枝, 佐々木直樹

      化学とマイクロ・ナノシステム学会第29回研究会  5 2014 

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    • 水性二相系を用いたマイクロチップ交流曇点抽出法の開発

      安達尭史, 佐々木直樹

      日本化学会第94春季年会  3 2014 

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    • 微小循環マイクロモデルの開発

      佐藤美和, 佐々木直樹, 平川聡史, 佐藤香枝

      化学とマイクロ・ナノシステム学会第28回研究会  12 2013 

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    • マイクロチップを用いたフロー培養法における細胞増殖

      佐藤美和, 佐々木直樹, 平川聡史, 佐藤香枝

      第33回キャピラリー電気泳動シンポジウム  11 2013 

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    • マイクロチップ交流曇点抽出法の開発

      佐々木直樹

      第33回キャピラリー電気泳動シンポジウム  11 2013 

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    • 血管・リンパ管マイクロデバイスの構築と特性評価

      佐藤美和, 佐々木直樹, 平川聡史, 佐藤香枝

      第3回CSJ化学フェスタ2013  10 2013 

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    • CHARACTERIZATION OF NANOPARTICLE PERMEABILITY ON A MEMBRANE-INTEGRATED MICROFLUIDIC DEVICE International conference

      Naoki Sasaki, Mariko Tatanou, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, Kae Sato

      The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)  10 2013 

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    • DEVELOPMENT OF AN EX-VIVO LYMPHATIC VASCULAR MODEL International conference

      Miwa Sato, Naoki Sasaki, Kiichi Sato, Satoshi Hirakawa, Kae Sato

      The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)  10 2013 

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    • マイクロ流体デバイスを用いる ナノ粒子の透過性評価

      佐々木直樹, 多多納麻里子, 安楽泰孝, 岸村顕広, 片岡一則, 城潤一郎, 青木伊知男, 佐藤香枝

      日本分析化学会第62年会  9 2013 

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    • CHARACTERIZATION OF A MICROFLUIDIC IN VITRO MODEL OF LYMPHATIC VESSEL International conference

      Miwa Sato, Naoki Sasaki, Satoshi Hirakawa, Kae Sato

      RSC Tokyo International Conference 2013  9 2013 

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    • CHARACTERIZATION OF NANOPARTICLE PERMEABILITY ON MEMBRANE-INTEGRATED MICROFLUIDIC DEVICES International conference

      Naoki Sasaki, Mariko Tatanou, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, Kae Sato

      RSC Tokyo International Conference 2013  9 2013 

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    • OPTIMIZATION OF MICROFLUIDIC-BASED IN SITU PADLOCK ROLLING CIRCLE AMPLIFICATION FOR MITOCHONDRIAL DNA ANALYSIS International conference

      Arisa Kuroda, Naoki Sasaki, Mats Nilsson, Kae Sato

      RSC Tokyo International Conference 2013  9 2013 

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    • 多孔膜集積マイクロ流体デバイスを用いたDDSナノ粒子の透過性評価

      佐々木直樹, 多多納麻里子, 安楽泰孝, 岸村顕広, 片岡一則, 佐藤香枝

      化学とマイクロ・ナノシステム学会第27回研究会  5 2013 

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    • 血管・リンパ管内皮細胞のマイクロチップ培養

      佐藤美和, 佐々木直樹, 平川聡史, 佐藤香枝

      化学とマイクロ・ナノシステム学会第27回研究会  5 2013 

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    • 光架橋反応を利用する細胞パターニングと細胞内DNA分析への応用 Invited

      佐々木直樹, 井須安里, 石井怜奈, 佐藤香枝

      第26回化学とマイクロ・ナノシステム研究会  10 2012 

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    • 分子・細胞・組織分析のためのマイクロ流体デバイス Invited

      佐々木直樹

      集積マイクロシステム研究センター研究会  10 2012 

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    • Mechanistic characterization of alternating current cloud point extraction in a microchannel: extraction under physiological temperature International conference

      Naoki SASAKI, Azusa TAKEMURA, Kae SATO

      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2012)  10 2012 

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    • Photo-immobilization of cells for in situ DNA analysis International conference

      Naoki SASAKI, Anri ISU, Reina ISHII, Kae SATO

      The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2012)  10 2012 

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    • 創薬研究のためのバイオマイクロ流体デバイス Invited

      佐々木直樹

      第23回新薬創製談話会  9 2012 

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    • MICROCHIP-BASED IN-SITU PADLOCK/ROLLING CIRCLE AMPLIFICATION (MICRO-RCA) SYSTEM FOR SINGLE DNA COUNTING IN A CELL International conference

      Arisa KURODA, Naoki SASAKI, Kae SATO

      RSC Tokyo International Conference 2012  9 2012 

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    • MICROFLUIDIC DEVICES FOR PERMEABILITY ASSAYS OF LYMPHATIC ENDOTHELIAL CELLS International conference

      Miwa SATO, Naoki SASAKI, Kae SATO

      RSC Tokyo International Conference 2012  9 2012 

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    • PHOTOCROSSLINKING OF CELLS TO A GLASS SUBSTRATE FOR IN SITU DNA ANALYSIS International conference

      Naoki SASAKI, Eri YOSHIDA, Anri ISU, Reina ISHII, Kae SATO

      RSC Tokyo International Conference 2012  9 2012 

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    • 光架橋反応を利用したガラス基板への細胞固定化と細胞内DNA分析への応用

      佐々木直樹, 井須安里, 石井怜奈, 佐藤香枝

      第25回化学とマイクロ・ナノシステム研究会  5 2012 

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    • 光架橋反応を利用したガラス基板への細胞固定化法の開発

      佐々木直樹, 井須安里, 佐藤香枝

      第72回分析化学討論会  5 2012 

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    • 手のひらサイズのマイクロチップ細胞培養システム

      佐々木直樹, 新城美佳, 平川聡史, 西中正弘, 田中陽, 馬渡和真, 北森武彦, 佐藤香枝

      第25回化学とマイクロ・ナノシステム研究会  5 2012 

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    • 微小循環マイクロモデルの開発 Invited

      佐藤香枝, 佐々木直樹

      第89回日本生理学会大会  3 2012 

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    • マイクロチップを用いた細胞内Padlock/RCA法の開発

      第2回CSJ化学フェスタ  2012 

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    • マイクロチップ送液システムを用いたPadlock/RCA法によるDNA分析

      日本分析化学会第61年会  2012 

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    • リンパ管内皮細胞のマイクロデバイス培養と細胞間接着分子の観察

      第2回CSJ化学フェスタ  2012 

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    • 細胞内Padlock/RCA のためのマイクロチップ送液システムの開発

      第32回キャピラリー電気泳動シンポジウム  2012 

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    • 交流動電現象を利用するマイクロ流体デバイスの開発とバイオ分析への応用 Invited

      佐々木直樹

      日本女子大学理学セミナー  10 2011 

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    • Counting single DNA molecule by on-bead rolling circle amplification for quantitative analyses International conference

      Reina ISHII, Naoki SASAKI, Kiichi SATO, Kazuma MAWATARI, Mats NILSSON, Takehiko KITAMORI, Kae SATO

      hemistry and Life Sciences (MicroTAS2011)  10 2011 

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    • Development of a microfluidic southern hybridization analysis system using microbeads International conference

      Kae SATO, Mami NISHIKAWA, Naoki SASAKI, Kiichi SATO

      The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2011)  10 2011 

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    • Mechanistic investigation of alternating current cloud point extraction in a microchannel International conference

      Naoki SASAKI, Azusa TAKEMURA, Kae SATO

      The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2011)  10 2011 

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    • 交流曇点抽出法による生体膜分子のオンチップ濃縮機構の解析

      佐々木直樹, 竹村梓, 佐藤香枝

      日本分析化学会第60年会  9 2011 

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    • 交流曇点抽出法によるオンチップ生体膜分子濃縮の特性解析

      佐々木直樹, 竹村梓, 佐藤香枝

      第23回化学とマイクロ・ナノシステム研究会  6 2011 

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    • Experimental characterization of alternating current cloud point extraction International conference

      Naoki SASAKI, Azusa TAKEMURA, Kae SATO

      IUPAC International Congress on Analytical Sciences 2011  5 2011 

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    • Single DNA Molecule Detection by On-Bead Rolling Circle Amplification for Efficient Detection International conference

      Naoki SASAKI, Reina ISHII, Yuri KITAMURA, Kiichi SATO, Kazuma MAWATARI, Mats NILSSON, Takehiko KITAMORI, Kae SATO

      IUPAC International Congress on Analytical Sciences 2011  5 2011 

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    • 交流曇点抽出法による生体膜分子のオンチップ濃縮

      佐々木直樹, 竹村梓, 佐藤香枝

      第4回ナノバイオ若手ネットワーキングシンポジウム  3 2011 

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    • 交流動電現象を利用するオンチップバイオ分析 Invited

      佐々木直樹

      日本女子大学理科縦の会  2 2011 

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    • マイクロビーズを用いたPadlock/RCA法の開発

      第23回化学とマイクロ・ナノシステム研究会  2011 

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    • マイクロビーズ電気泳動によるサザンハイブリダイゼーション法の開発

      第23回化学とマイクロ・ナノシステム研究会  2011 

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    • マイクロペリスタリックポンプを用いたマイクロチップ細胞培養

      第23回化学とマイクロ・ナノシステム研究会  2011 

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    • マイクロ流体デバイスを用いた血管内皮細胞の培養

      第1回CSJ化学フェスタ  2011 

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    • Alternating current cloud point extraction on a microfluidic chip for preconcentration of membrane-associated biomolecules International conference

      Naoki SASAKI, Kazuo HOSOKAWA, Mizuo MAEDA

      2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010)  12 2010 

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    • 流れを利用する異種細胞配置・融合用マイクロ流路のアレイ化

      佐々木直樹, 森崎良, Jiansheng Gong, 細川和生, 伊藤嘉浩, 前田瑞夫

      第22回化学とマイクロ・ナノシステム研究会  11 2010 

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    • Pairing and fusion of heterotypic cells in a microchannel International conference

      Naoki SASAKI, Jiangsheng GONG, Kazuo HOSOKAWA, Mizuo MAEDA, Yoshihiro ITO

      The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2010)  10 2010 

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    • Single DNA molecule detection by on-bead rolling circle amplification using microchip for efficient detection International conference

      Kae SATO, Yuri KITAMURA, Naoki SASAKI, Kiichi SATO, Kazuma MAWATARI, Mats NILSSON, Takehiko KITAMORI

      The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2010)  10 2010 

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    • マイクロビーズを用いたPadlock/RCA法の開発

      北村友理, 佐々木直樹, 佐藤香枝

      第21回化学とマイクロ・ナノシステム研究会  6 2010 

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    • マイクロ流路内異種細胞配置・融合法の開発

      佐々木直樹, Jiansheng Gong, 細川和生, 前田瑞夫, 伊藤嘉浩

      第21回化学とマイクロ・ナノシステム研究会  6 2010 

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    • マイクロビーズPadlock/RCA法による微量DNA分析

      東京コンファレンス2010  2010 

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    • マイクロ流体デバイスを用いるES細胞-体細胞融合法の開発

      第3回ナノバイオ若手ネットワーキングシンポジウム  2010 

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    • 細胞融合実験用マイクロ流体チップの開発

      日本化学会 第4回関東支部大会  2010 

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    • 交流動電現象のオンチップバイオ分析への応用 Invited

      佐々木直樹

      第4回バイオ医工学シンポジウム  3 2009 

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    • Analysis of fluid motion in alternating current cloud point extraction

      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2009 

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    • 交流動電現象を利用するオンチップ曇点抽出

      東京コンファレンス2009  2009 

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    • 交流動電相分離とその濃縮操作への応用(3):流体挙動の解析

      第19回化学とマイクロ・ナノシステム研究会  2009 

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    • Analysis of fluid motion in alternating current cloud point extraction

      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2009 

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    • AC electrokinetic phase separation, focusing and concentration in microchannel

      The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2008 

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    • 交流動電相分離とその濃縮操作への応用

      第17回化学とマイクロ・ナノシステム研究会  2008 

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    • 交流動電相分離とその濃縮操作への応用(2):濃縮機構の検討

      日本分析化学会第57年会  2008 

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    • AC electrokinetic phase separation, focusing and concentration in microchannel

      The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2008 

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    • Rapid mixing by AC electrothermal flow

      The 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2007 

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    • 交流熱動電流を利用した高速マイクロミキサー

      第15回化学とマイクロ・ナノシステム研究会  2007 

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    • Rapid mixing by AC electrothermal flow

      The 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2007 

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    • AC electroosmotic micromixer: viscosity and ionic strength dependence of mixing time

      The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2006 

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    • Fluid Mixing by AC Electroosmosis in Microchannel

      International Electrokinetics Conference  2006 

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    • Performance Evaluations of Fluid Mixing by AC Electroosmosis in Microchannel

      International Electrokinetics Conference  2006 

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    • 交流動電現象のマイクロ化学プロセスへの応用

      日本分析化学会第55年会  2006 

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    • 交流電気浸透を利用した高速マイクロミキサー(4):溶媒依存性の検討

      第13回化学とマイクロ・ナノシステム研究会  2006 

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    • AC electroosmotic micromixer: viscosity and ionic strength dependence of mixing time

      The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2006 

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      Presentation type:Poster presentation  

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    • Fluid Mixing by AC Electroosmosis in Microchannel

      International Electrokinetics Conference  2006 

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    • Performance Evaluations of Fluid Mixing by AC Electroosmosis in Microchannel

      International Electrokinetics Conference  2006 

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    • AC Electroosmotic Mixer for Rapid Mixing in Microchannel on Chip

      Pacifichem 2005  2005 

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    • Rapid mixing based on AC electroosmosis in microchannel

      The 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2005 

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    • AC Electroosmotic Mixer for Rapid Mixing in Microchannel on Chip

      Pacifichem 2005  2005 

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    • Rapid mixing based on AC electroosmosis in microchannel

      The 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2005 

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    Teaching Experience

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      ナノサイエンス ( 東洋大学 )

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      先端化学 ( 東洋大学 )

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      分析化学応用特論1 ( 立教大学 )

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      分析化学1 ( 立教大学 )

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      化学II ( 東洋大学 )

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      化学の最前線 ( 立教大学 )

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      化学実験 ( 東洋大学 )

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      化学実験A ( 立教大学 )

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      化学実験B ( 立教大学 )

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      化学実験C ( 立教大学 )

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    •  
      化学概論実験 ( 日本女子大学 )

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      安全化学 ( 東洋大学 )

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    •  
      専門化学実験III ( お茶の水女子大学 )

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    •  
      物理化学I ( 東洋大学 )

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      物理化学II ( 東洋大学 )

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      物理化学III ( 東洋大学 )

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      物理化学実験 ( 日本女子大学 )

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    Professional Memberships

    •  
      THE CHEMICAL SOCIETY OF JAPAN

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      THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY

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      THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS

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    •  
      THE SOCIETY OF POLYMER SCIENCE, JAPAN

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    Research Projects

    • ナノ薬剤評価用マイクロ腫瘍組織モデルの開発

      日本学術振興会  科学研究費助成事業 基盤研究(C) 

      佐々木 直樹, 岸村 顕広

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      4 2019 - 3 2022

      Grant number:19K05236

      Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

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    • White box testing of nanomedicines on a microfluidic device

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) 

      Sasaki Naoki

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      4 2015 - 3 2018

      Grant number:15K04640

      Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

      Nanoparticles have been widely utilized to deliver drugs from blood vessels to tumor tissues. In this study, we developed a microfluidic device to clarify nanoparticle characteristics required for efficient drug delivery. A consumer laser cutter was employed to fabricate the device. Tumor cells and vascular endothelial cells were co-cultured on a membrane-integrated microfluidic device. Permeation of nanoparticles through the endothelial monolayer increased in the presence of tumor cells.

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    • Rapid, sensitive, and on-site detection of bacteria using DNA amplidication on an electrode-integrated microfluidic device

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) 

      SASAKI Naoki

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      4 2013 - 3 2015

      Grant number:25790037

      Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

      This project is a proof-of concept study of rapid, sensitive, and on-site detection of bacteria using DNA amplidication on an electrode-integrated microfluidic device. Concentration of samples under alternating electric fields in a microfluidic channel was demonstrated. DNA detection by rolling circle amplification (RCA) was also demonstrated. Poly(ethylene glycol) (PEG) was used to facilitate RCA by molecular crowding effects. The number of RCA products increased in the presence of PEG.

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    • Microfluidic device for stem cell communication analysis

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B) 

      SASAKI Naoki

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      2009 - 2010

      Grant number:21710131

      Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

      Stem cells can replicate themselves and differentiate into various types of somatic cells. It is highly important to clarify such mechanisms from a scientific point of view and for the application in the area of regenerative medicine. We have developed microfluidic devices to analyze the communication between stem cells. Controlled pairing of embryonic stem cells with somatic cells has been demonstrated. An arrayed microfluidic device which has multiple microchannels has enabled high throughput operations.

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    • 微細加工技術を駆使した細胞質注入による体細胞リプログラミング法の確立

      日本学術振興会  科学研究費助成事業 挑戦的萌芽研究 

      伊藤 嘉浩, 細川 和生, 佐々木 直樹

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      2009

      Grant number:21650122

      Grant amount:\3200000 ( Direct Cost: \3200000 )

      京都大学の多田らによって2001年に、体細胞とES細胞を細胞融合する手法でも体細胞はリプログラミングされることが、マウスを使って報告されると(Curr.Biol., 2001, 11, 1553-1158)、2005年にはハーバード大学でヒトES細胞を使っての成功が報じられた(Science, 2005, 309, 1369-1373)。しかし、この方法で作成する細胞は4倍体で、正常2倍体とは異なる。山中らはこの報告に触発され、ES細胞中にある遺伝子の注入による体細胞のリプログラミングを実現した。しかし、遺伝子導入のためにレトロウイルスを用いており、遺伝子治療での課題は未解決のままである。
      そこで、本研究では、体細胞とES細胞を融合させ、細胞質交換はあるものの核の移動が起こらない数マイクロメートルの孔を介して細胞を対峙させ、細胞融合を行い2倍体の体細胞由来ES様細胞の構築を目指した体細胞とES細胞を所定の位置にポジショニングするためのマイクロ流路を組み込んだマイクロデバイスを設計し、作成した。ES細胞と体細胞の融合には、HVJエンベロープを用いた。細胞融合を行ったところ、20分後にはES細胞から体細胞へグリーン・フルオレセンス・センスプロテイン(GFP)が移動し、両者の細胞核はそのまま移動しいことが蛍光顕微鏡で確認することができ、当初計画したデバイスが作成できた。さらにマルチ流路型デバイスも作成し、同時に32か所で細胞融合の試行が可能となった。ただし、本研究期間内だけでは、この細胞質の移動による体細胞のリプログラミングまでは観察できなかった。

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    Industrial property rights

    • 光架橋反応を利用したガラス基板への細胞固定化法

      佐藤香枝, 佐々木直樹

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      Application no:特願2012-83371  Date applied:31 3 2012

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    • マイクロミキサーおよび流体の撹拌方法並びに流体の混合方法

      北森武彦, 金幸夫, 佐々木直樹

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      Application no:特願2006-243584  Date applied:8 9 2006

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    • マイクロミキサーおよび流体の撹拌方法並びに流体の混合方法

      北森武彦, 金幸夫, 佐々木直樹

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      Application no:特願2005-148719  Date applied:20 5 2005

      Patent/Registration no:特許4590557  Date issued:1 12 2010

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    • 流体混合装置

      北森武彦, 金幸夫, 佐々木直樹

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      Application no:特願2005-148720  Date applied:20 5 2005

      Patent/Registration no:特許4590558  Date issued:1 12 2010

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