Updated on 2024/02/02

写真b

 
SUZUKI Shota
 
*Items subject to periodic update by Rikkyo University (The rest are reprinted from information registered on researchmap.)
Affiliation*
College of Science
Title*
Assistant Professor
Degree
Doctor of Science ( 3 2014   Rikkyo University )
Campus Career*
  • 3 2020 - Present 
    College of Science   Assistant Professor
 

Research Areas

  • Life Science / Genetics

  • Life Science / Molecular biology

  • Life Science / Genome biology

Research History

  • 3 2020 - Present 
    立教大学 理学部 生命理学科   助教

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  • 4 2018 - 3 2020 
    法政大学 マイクロ・ナノテクノロジー研究センター   研究員

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  • 4 2016 - 3 2018 
    法政大学 マイクロ・ナノテクノロジー研究センター   P・D

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  • 4 2014 - 3 2016 
    東京大学 生物生産工学研究センター   研究員

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Education

  • 4 2011 - 3 2014 
    立教大学 理学研究科 生命理学専攻 博士後期課程

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  • 4 2009 - 3 2011 
    立教大学 理学研究科 生命理学専攻 博士前期課程

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  • - 3 2009 
    立教大学 理学部 生命理学科

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Papers

  • New Bacillus subtilis vector, pSSβ, as genetic tool for site-specific integration and excision of cloned DNA, and prophage elimination Peer-reviewed

    Shota Suzuki, Sachie Osada, Daisuke Imamura, Tsutomu Sato

    The Journal of General and Applied Microbiology68 ( 2 ) 71 - 78   2022

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Microbiology Research Foundation  

    DOI: 10.2323/jgam.2021.10.004

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  • Compatibility of Site-Specific Recombination Units between Mobile Genetic Elements Peer-reviewed

    Shota Suzuki, Miki Yoshikawa, Daisuke Imamura, Kimihiro Abe, Patrick Eichenberger, Tsutomu Sato

    iScience23 ( 1 ) 100805 - 100805   1 2020

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.isci.2019.100805

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  • C-terminal regulatory domain of the ε subunit of Fo F1 ATP synthase enhances the ATP-dependent H+ pumping that is involved in the maintenance of cellular membrane potential in Bacillus subtilis. Peer-reviewed International journal

    Genki Akanuma, Tomoaki Tagana, Maho Sawada, Shota Suzuki, Tomohiro Shimada, Kan Tanaka, Fujio Kawamura, Yasuyuki Kato-Yamada

    MicrobiologyOpen8 ( 8 ) e00815   8 2019

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    Language:English   Publishing type:Research paper (scientific journal)  

    The ε subunit of Fo F1 -ATPase/synthase (Fo F1 ) plays a crucial role in regulating Fo F1 activity. To understand the physiological significance of the ε subunit-mediated regulation of Fo F1 in Bacillus subtilis, we constructed and characterized a mutant harboring a deletion in the C-terminal regulatory domain of the ε subunit (ε∆C ). Analyses using inverted membrane vesicles revealed that the ε∆C mutation decreased ATPase activity and the ATP-dependent H+ -pumping activity of Fo F1 . To enhance the effects of ε∆C mutation, this mutation was introduced into a ∆rrn8 strain harboring only two of the 10 rrn (rRNA) operons (∆rrn8 ε∆C mutant strain). Interestingly, growth of the ∆rrn8 ε∆C mutant stalled at late-exponential phase. During the stalled growth phase, the membrane potential of the ∆rrn8 ε∆C mutant cells was significantly reduced, which led to a decrease in the cellular level of 70S ribosomes. The growth stalling was suppressed by adding glucose into the culture medium. Our findings suggest that the C-terminal region of the ε subunit is important for alleviating the temporal reduction in the membrane potential, by enhancing the ATP-dependent H+ -pumping activity of Fo F1 .

    DOI: 10.1002/mbo3.815

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  • Dynamic changes in lysine acetylation and succinylation of the elongation factor Tu in Bacillus subtilis Peer-reviewed

    Shota Suzuki, Naoko Kondo, Minoru Yoshida, Makoto Nishiyama, Saori Kosono

    Microbiology165 ( 1 ) 65 - 77   1 1 2019

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Microbiology Society  

    DOI: 10.1099/mic.0.000737

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  • Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis Peer-reviewed

    Genki Akanuma, Yuka Kazo, Kazumi Tagami, Hirona Hiraoka, Koichi Yano, Shota Suzuki, Ryo Hanai, Hideaki Nanamiya, Yasuyuki Kato-Yamada, Fujio Kawamura

    Microbiology162 ( 3 ) 448 - 458   1 3 2016

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    Publishing type:Research paper (scientific journal)   Publisher:Microbiology Society  

    DOI: 10.1099/mic.0.000234

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  • Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source Peer-reviewed

    Saori Kosono, Masaru Tamura, Shota Suzuki, Yumi Kawamura, Ayako Yoshida, Makoto Nishiyama, Minoru Yoshida

    PLOS ONE10 ( 6 ) e0131169   6 2015

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    Lysine residues can be post-translationally modified by various acyl modifications in bacteria and eukarya. Here, we showed that two major acyl modifications, acetylation and succinylation, were changed in response to the carbon source in the Gram-positive model bacterium Bacillus subtilis. Acetylation was more common when the cells were grown on glucose, glycerol, or pyruvate, whereas succinylation was upregulated when the cells were grown on citrate, reflecting the metabolic states that preferentially produce acetyl-CoA and succinyl-CoA, respectively. To identify and quantify changes in acetylation and succinylation in response to the carbon source, we performed a stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomic analysis of cells grown on glucose or citrate. We identified 629 acetylated proteins with 1355 unique acetylation sites and 204 succinylated proteins with 327 unique succinylation sites. Acetylation targeted different metabolic pathways under the two growth conditions: branched-chain amino acid biosynthesis and purine metabolism in glucose and the citrate cycle in citrate. Succinylation preferentially targeted the citrate cycle in citrate. Acetylation and succinylation mostly targeted different lysine residues and showed a preference for different residues surrounding the modification sites, suggesting that the two modifications may depend on different factors such as characteristics of acyl-group donors, molecular environment of the lysine substrate, and/or the modifying enzymes. Changes in acetylation and succinylation were observed in proteins involved in central carbon metabolism and in components of the transcription and translation machineries, such as RNA polymerase and the ribosome. Mutations that modulate protein acylation affected B. subtilis growth. A mutation in acetate kinase (ackA) increased the global acetylation level, suggesting that acetyl phosphate-dependent acetylation is common in B. subtilis, just as it is in Escherichia coli. Our results suggest that acyl modifications play a role in the physiological adaptations to changes in carbon nutrient availability of B. subtilis.

    DOI: 10.1371/journal.pone.0131169

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  • Purification of 70S Ribosomes from Bacillus subtilis Peer-reviewed

    Shota Suzuki, Genki Akanuma, Fujio Kawamura

    BIO-PROTOCOL5 ( 7 )   2015

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   Publisher:Bio-Protocol, LLC  

    DOI: 10.21769/bioprotoc.1432

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  • Defect in the Formation of 70S Ribosomes Caused by Lack of Ribosomal Protein L34 Can Be Suppressed by Magnesium Peer-reviewed

    Genki Akanuma, Ako Kobayashi, Shota Suzuki, Fujio Kawamura, Yuh Shiwa, Satoru Watanabe, Hirofumi Yoshikawa, Ryo Hanai, Morio Ishizuka

    JOURNAL OF BACTERIOLOGY196 ( 22 ) 3820 - 3830   11 2014

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the Delta rpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored the growth rate of the Delta rpmH strain. Either the disruption of yhdP, which is thought to be involved in the efflux of Mg2+, or overexpression of mgtE, which plays a major role in the import of Mg2+, could suppress defects in both the formation of the 70S ribosome and growth caused by the absence of L34. Interestingly, the Mg2+ content was lower in the Delta rpmH cells than in the wild type, and the Mg2+ content in the Delta rpmH cells was restored by either the disruption of yhdP or overexpression of mgtE. In vitro experiments on subunit association demonstrated that 50S subunits that lacked L34 could form 70S ribosomes only at a high concentration of Mg2+. These results showed that L34 is required for efficient 70S ribosome formation and that L34 function can be restored partially by Mg2+. In addition, the Mg2+ content was consistently lower in mutants that contained significantly reduced amounts of the 70S ribosome, such as the Delta rplA (L1) and Delta rplW (L23) strains and mutant strains with a reduced number of copies of the rrn operon. Thus, the results indicated that the cellular Mg2+ content is influenced by the amount of 70S ribosomes.

    DOI: 10.1128/JB.01896-14

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  • Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2 Peer-reviewed

    Shota Suzuki, Osamu Tanigawa, Genki Akanuma, Hideaki Nanamiya, Fujio Kawamura, Kazumi Tagami, Naofumi Nomura, Teppei Kawabata, Yasuhiko Sekine

    MICROBIOLOGY-SGM160 ( Pt 6 ) 1040 - 1053   6 2014

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC GENERAL MICROBIOLOGY  

    A temperature-sensitive mutation in rplB, designated rplB142, encodes a missense mutation at position 142 [His (CAT) to Leu (CTT)] of Bacillus subtilis ribosomal protein L2. The strain carrying the mutation grew more slowly than the wild-type, even at low temperatures, probably due to the formation of defective 70S ribosomes and the accumulation of incomplete 50S subunits (50S* subunits). Gel analysis indicated that amounts of L2 protein and also of L16 protein were reduced in ribosomes prepared from the rplB142 mutant 90 min after increasing the growth temperature to 45 degrees C. These results suggest that the assembly of the L16 protein into the 50S subunit requires the native L2 protein. The H142L mutation in the defective L2 protein affected sporulation as well as growth, even at the permissive temperature. A suppressor mutation that restored both growth and sporulation of the rplB142 mutant at low temperature was identified as a single base deletion located immediately upstream of the yaaA gene that resulted in an increase in its transcription. Furthermore, genetic analysis showed that enhanced synthesis of YaaA restores the functionality of L2 (H142L) by facilitating its assembly into 50S subunits.

    DOI: 10.1099/mic.0.076463-0

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  • Cell motility and biofilm formation in Bacillus subtilis are affected by the ribosomal proteins, S11 and S21 Peer-reviewed

    Hiraku Takada, Masato Morita, Yuh Shiwa, Ryoma Sugimoto, Shota Suzuki, Fujio Kawamura, Hirofumi Yoshikawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY78 ( 5 ) 898 - 907   5 2014

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Bacillus subtilis differentiates into various cellular states in response to environmental changes. It exists in two states during the exponential growth phase: motile cells and connected chains of sessile cells. Here, we identified new regulators of cell motility and chaining, the ribosomal proteins S21 (rpsU) and S11 (rpsK). Their mutants showed impaired cell motility (observed in a laboratory strain) and robust biofilm formation (observed in an undomesticated strain). The two major operons for biofilm formation, tapA-sipW-tasA and epsA-O, were strongly expressed in the rpsU mutant, whereas the flagellin-encoding hag gene and other SigD-dependent motility regulons were not. Genetic analysis revealed that the mutation of remA, the transcriptional activator of the eps operon, is epistatic to that of rpsU, whereas the mutation of antagonistic regulators of SinR is not. Our studies demonstrate that S11 and S21 participate in the regulation of bistability via the RemA/RemB pathway.

    DOI: 10.1080/09168451.2014.915729

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  • Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis Peer-reviewed

    Koichi Yano, Tetsuya Wada, Shota Suzuki, Kazumi Tagami, Takashi Matsumoto, Yuh Shiwa, Taichiro Ishige, Yasuhiro Kawaguchi, Kenta Masuda, Genki Akanuma, Hideaki Nanamiya, Hironori Niki, Hirofumi Yoshikawa, Fujio Kawamura

    MICROBIOLOGY-SGM159 ( Pt 11 ) 2225 - 2236   11 2013

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC GENERAL MICROBIOLOGY  

    The number of copies of rRNA (rrn) operons in a bacterial genome differs greatly among bacterial species. Here we examined the phenotypic effects of variations in the number of copies of rRNA genes in the genome of Bacillus subtilis by analysis of eight mutant strains constructed to carry from two to nine copies of the rrn operon. We found that a decrease in the number of copies from ten to one increased the doubling time, and decreased the sporulation frequency and motility. The maximum levels for transformation activity were similar among the strains, although the competence development was significantly delayed in the strain with a single rrn operon. Normal sporulation only occurred if more than four copies of the rrn operon were present, although ten copies were needed for vegetative growth after germination of the spores. This behaviour was seen even though the intracellular level of ribosomes was similar among strains with four to ten copies of the rrn operon. Furthermore, ten copies of the rrn operon were needed for the highest swarming activity. We also constructed 21 strains that carried all possible combinations of two copies of the rrn operons, and found that these showed a range of growth rates and sporulation frequencies that all fell between those recorded for strains with one or three copies of the rrn operon. The results suggested that the copy number of the rrn operon has a major influence on cellular processes such as growth rate and sporulation frequency.

    DOI: 10.1099/mic.0.067025-0

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  • Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis Peer-reviewed

    Genki Akanuma, Shota Suzuki, Koichi Yano, Hideaki Nanamiya, Yousuke Natori, Eri Namba, Kazuya Watanabe, Kazumi Tagami, Takuya Takeda, Yuka Iizuka, Ako Kobayashi, Morio Ishizuka, Hirofumi Yoshikawa, Fujio Kawamura

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY59 ( 2 ) 105 - 117   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MICROBIOL RES FOUNDATION  

    We introduced single mutations into the rplC and rpsJ genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium Bacillus subtilis, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45 degrees C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.

    DOI: 10.2323/jgam.59.105

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  • Inactivation of Ribosomal Protein Genes in Bacillus subtilis Reveals Importance of Each Ribosomal Protein for Cell Proliferation and Cell Differentiation Peer-reviewed

    Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura

    JOURNAL OF BACTERIOLOGY194 ( 22 ) 6282 - 6291   11 2012

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    Among the 57 genes that encode ribosomal proteins in the genome of Bacillus subtilis, a Gram-positive bacterium, 50 genes were targeted by systematic inactivation. Individual deletion mutants of 16 ribosomal proteins (L1, L9, L15, L22, L23, L28, L29, L32, L33.1, L33.2, L34, L35, L36, S6, S20, and S21) were obtained successfully. In conjunction with previous reports, 22 ribosomal proteins have been shown to be nonessential in B. subtilis, at least for cell proliferation. Although several mutants that harbored a deletion of a ribosomal protein gene did not show any significant differences in any of the phenotypes that were tested, various mutants showed a reduced growth rate and reduced levels of 70S ribosomes compared with the wild type. In addition, severe defects in the sporulation frequency of the Delta rplA (L1) mutant and the motility of the Delta rpsU (S21) mutant were observed. These data provide the first evidence in B. subtilis that L1 and S21 are required for the progression of cellular differentiation.

    DOI: 10.1128/JB.01544-12

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  • Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp(0) mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome Peer-reviewed

    Kazumi Tagami, Hideaki Nanamiya, Yuka Kazo, Marie Maehashi, Shota Suzuki, Eri Namba, Masahiro Hoshiya, Ryo Hanai, Yuzuru Tozawa, Takuya Morimoto, Naotake Ogasawara, Yasushi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Masaki Yoshida, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yoshiaki Ohashi, Fujio Kawamura

    MICROBIOLOGYOPEN1 ( 2 ) 115 - 134   6 2012

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    To elucidate the biological functions of small (p)ppGpp synthetases YjbM and YwaC of Bacillus subtilis, we constructed RIK1059 and RIK1066 strains carrying isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible yjbM and ywaC genes, respectively, in the Delta relA Delta yjbM Delta ywaC triple mutant background. While the uninduced and IPTG-induced RIK1059 cells grew similarly in LB medium, the growth of RIK1066 cells was arrested following the addition of IPTG during the early exponential growth phase. Induction of YwaC expression by IPTG also severely decreased the intracellular GTP level and drastically altered the transcriptional profile in RIK1066 cells. Sucrose density gradient centrifugation analysis of the ribosomal fractions prepared from the IPTG-induced RIK1066 cells revealed three peaks corresponding to 30S, 50S, and 70S ribosome particles, and also an extra peak. Electron microscope studies revealed that the extra peak fraction contained dimers of 70S ribosomes, which were similar to the Escherichia coli 100S ribosomes. Proteomic analysis revealed that the 70S dimer contained an extra protein, YvyD, in addition to those found in the 70S ribosome. Accordingly, strain resulting from the disruption of the yvyD gene in the RIK1066 cells was unable to form 70S dimers following IPTG induction, indicating that YvyD is required for the formation of these dimers in B. subtilis.

    DOI: 10.1002/mbo3.16

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  • Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation Peer-reviewed

    Hideaki Nanamiya, Makiko Sato, Kenta Masuda, Mikiko Sato, Tetsuya Wada, Shota Suzuki, Yousuke Natori, Masato Katano, Genki Akanuma, Fujio Kawamura

    MICROBIOLOGY-SGM156 ( Pt 10 ) 2944 - 2952   10 2010

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC GENERAL MICROBIOLOGY  

    The number of copies of rRNA genes in bacterial genomes differs greatly among bacterial species. It is difficult to determine the functional significance of the heterogeneity of each rRNA operon fully due to the existence of multiple rRNA operons and because the sequence heterogeneity among the rRNA genes is extremely low. To overcome this problem, we sequentially deleted the ten rrn operons of Bacillus subtilis and constructed seven mutant strains that each harboured a single rrn operon (either rrnA, B, D, E, I, J or O) in their genome. The growth rates and sporulation frequencies of these mutants were reduced drastically compared with those of the wild-type strain, and this was probably due to decreased levels of ribosomes in the mutants. Interestingly, the ability to sporulate varied significantly among the mutant strains. These mutants have proved to be invaluable in our initial attempts to reveal the functional significance of the heterogeneity of each rRNA operon.

    DOI: 10.1099/mic.0.035295-0

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  • Transcription Activity of Individual rrn Operons in Bacillus subtilis Mutants Deficient in (p)ppGpp Synthetase Genes, relA, yjbM, and ywaC Peer-reviewed

    Yousuke Natori, Kazumi Tagami, Kana Murakami, Sawako Yoshida, Osamu Tanigawa, Yoonsuh Moh, Kenta Masuda, Tetsuya Wada, Shota Suzuki, Hideaki Nanamiya, Yuzuru Tozawa, Fujio Kawamura

    JOURNAL OF BACTERIOLOGY191 ( 14 ) 4555 - 4561   7 2009

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    In Bacillus subtilis a null mutation of the relA gene, whose gene product is involved in the synthesis and/or hydrolysis of (p) ppGpp, causes a growth defect that can be suppressed by mutation(s) of yjbM and/or ywaC coding for small (p) ppGpp synthetases. All 35 suppressor mutations newly isolated were classified into two groups, either yjbM or ywaC, by mapping and sequencing their mutations, suggesting that there are no (p) ppGpp synthetases other than RelA, YjbM, and YwaC in B. subtilis. In order to understand better the relation between RelA and rRNA synthesis, we studied in the relA mutant the transcriptional regulation of seven rRNA operons (rrnO, -A, -J, -I, -E, -D, or -B) individually after integration of a promoter- and terminatorless cat gene. We identified the transcriptional start sites of each rrn operon (a G) and found that transcription of all rrn operons from their P1 promoters was drastically reduced in the relA mutant while this was almost completely restored in the relA yjbM ywaC triple mutant. Taken together with previous results showing that the intracellular GTP concentration was reduced in the relA mutant while it was restored in the triple mutant, it seems likely that continuous (p)ppGpp synthesis by YjbM and/or YwaC at a basal level causes a decrease in the amounts of intracellular GTP.

    DOI: 10.1128/JB.00263-09

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Misc.

  • 枯草菌リボソーム30Sサブユニットタンパク質RpsK,RpsUの機能解析

    高田啓, 盛田雅人, 杉本竜馬, 大竹俊平, 志波優, 鈴木祥太, 河村富士夫, 吉川博文, 吉川博文

    日本ゲノム微生物学会年会要旨集7th   2013

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  • 枯草菌リボソーム30Sサブユニットタンパク質RpsK,RpsUの機能解析

    高田啓, 盛田雅人, 杉本竜馬, 大竹俊平, 鈴木祥太, 志波優, 河村富士夫, 吉川博文, 吉川博文

    日本農芸化学会大会講演要旨集(Web)2013   2013

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  • Molecular genetic analysis of the function of rRNA in Bacillus subtilis

    Koichi Yano, Eri Namba, Rie Sekine, Shota Suzuki, Kazumi Tagami, Fujio Kawamura

    GENES & GENETIC SYSTEMS87 ( 6 ) 410 - 410   12 2012

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

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  • Development of high expression Bacillus subtilis system using a ribosome possessing an altered Shine-Dalgarno sequence

    Takuya Takeda, Koichi Yano, Shota Suzuki, Eri Nanba, Fujio Kawamura

    GENES & GENETIC SYSTEMS87 ( 6 ) 432 - 432   12 2012

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  • Functional analysis of mutants deleting the 16S rRNA helix 9 in Bacillus subtilis

    Shota Suzuki, Eri Namba, Takuya Takeda, Koichi Yano, Yasuhiko Sekine, Fujio Kawamura

    GENES & GENETIC SYSTEMS86 ( 6 ) 420 - 420   12 2011

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  • Assessment of the copy number of rRNA operons required for normal growth and sporulation in Bacillus subtilis

    Tetsuya Wada, Shota Suzuki, Masahiro Hoshiya, Fujio Kawamura

    GENES & GENETIC SYSTEMS85 ( 6 ) 421 - 421   12 2010

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  • Altering Helix 9 of 16S rRNA showed functional defects of a ribosome in B. subtilis

    Shota Suzuki, Tetsuya Wada, Fujio Kawamura

    GENES & GENETIC SYSTEMS85 ( 6 ) 422 - 422   12 2010

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

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  • Dimerization of ribosome was induced by treatment with various stresses in Bacillus subtilis

    Masahiro Hoshiya, Tetsuya Wada, Shota Suzuki, Yasusi Kageyama, Katsutoshi Ara, Katsuya Ozaki, Fujio Kawamura

    GENES & GENETIC SYSTEMS85 ( 6 ) 421 - 421   12 2010

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

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  • Defects in the rRNA deletion mutants harboring single rRNA operon can be suppressed by rRNA operon amplification

    Kenta Masuda, Kazumi Tagami, Tetsuya Wada, Shota Suzuki, Yuka Noguchi, Masahiro Hoshiya, Fujio Kawamura

    GENES & GENETIC SYSTEMS84 ( 6 ) 454 - 454   12 2009

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

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  • Isolation and characterization of suppressor mutants restoring the defects in growth and sporulation of multiple rRNA operon deletion mutants in Bacillus subtills

    Kenta Masuda, Shota Suzuki, Fujio Kawamura

    GENES & GENETIC SYSTEMS83 ( 6 ) 513 - 513   12 2008

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    Language:English   Publishing type:Research paper, summary (international conference)   Publisher:GENETICS SOC JAPAN  

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Teaching Experience

  • 11 2019 - 3 2020 
    分子生物学 ( 法政大学 )

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  • 4 2018 - 3 2020 
    科学実験(実習) ( 法政大学 )

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  • 4 2018 - 3 2020 
    生物学基礎 ( 法政大学 )

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