Updated on 2025/08/16

写真b

 
TACHIKAWA Hiroyuki
 
*Items subject to periodic update by Rikkyo University (The rest are reprinted from information registered on researchmap.)
Affiliation*
College of Sport and Wellness Department of Sport and Wellness
Graduate School of Sport and Wellness Master's Program in Sport and Wellness
Graduate School of Sport and Wellness Doctoral Program in Sport and Wellness
Title*
Professor
Degree
博士(農学) ( 東京大学 )
Contact information
Mail Address
Research Interests
  • lipid transfer

  • membrane traffic

  • Sporulation

  • Budding Yeast

  • membrane formation

  • Biological membrane

  • Campus Career*
    • 4 2023 - Present 
      College of Sport and Wellness   Department of Sport and Wellness   Professor
    • 4 2023 - Present 
      Graduate School of Sport and Wellness   Master's Program in Sport and Wellness   Professor
    • 4 2023 - Present 
      Graduate School of Sport and Wellness   Doctoral Program in Sport and Wellness   Professor
     

    Research Areas

    • Life Science / Applied molecular and cellular biology

    • Life Science / Applied microbiology

    Research History

    • 4 2023 - Present 
      Rikkyo University

      More details

    • 10 2009 - 3 2023 
      The University of Tokyo

      More details

    • 10 2004 - 9 2009 
      The University of Tokyo

      More details

    • 4 1993 - 9 2004 
      Tokyo University of Agriculture and Technology

      More details

    Education

    • 4 1990 - 3 1993 
      東京大学大学院   農学系研究科   農芸化学専攻博士課程

      More details

    • 4 1988 - 3 1990 
      東京大学大学院   農学系研究科   農芸化学専攻修士課程

      More details

    • - 3 1988 
      The University of Tokyo

      More details

    Committee Memberships

    • 12 2019 - 9 2021 
      酵母細胞研究会   第23回酵母合同シンポジウム実行委員会委員

      More details

    • 9 2019 - 5 2020 
      日本農芸化学会   本部選挙管理委員会委員

      More details

    • 12 2017 - 2 2020 
      日本農芸化学会   2019年度東京大会実行委員会委員

      More details

      Committee type:Academic society

      researchmap

    • 9 2017 - 5 2018 
      日本農芸化学会   本部選挙管理委員会委員

      More details

      Committee type:Academic society

      researchmap

    • 12 2016 - 10 2017 
      酵母遺伝学フォーラム   2017年度研究報告会実行委員

      More details

      Committee type:Academic society

      researchmap

    • 3 2013 - 2 2015 
      日本農芸化学会   2014年度東京大会実行委員会委員

      More details

      Committee type:Academic society

      researchmap

    • 12 2013 - 9 2014 
      酵母細胞研究会   第21回酵母合同シンポジウム実行委員会委員

      More details

      Committee type:Other

      researchmap

    ▼display all

    Papers

    • Application of yeast spores as β-glucan particles

      Guoyu Liu, Yan Yang, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi

      Particuology71   34 - 40   12 2022

      More details

      Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

      DOI: 10.1016/j.partic.2022.01.013

      researchmap

    • Receptor for advanced glycation end-products (RAGE) mediates phagocytosis in nonprofessional phagocytes. International journal

      Yan Yang, Guoyu Liu, Feng Li, Lucas B Carey, Changjin Sun, Kaiping Ling, Hiroyuki Tachikawa, Morihisa Fujita, Xiao-Dong Gao, Hideki Nakanishi

      Communications biology5 ( 1 ) 824 - 824   16 8 2022

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      In mammals, both professional phagocytes and nonprofessional phagocytes (NPPs) can perform phagocytosis. However, limited targets are phagocytosed by NPPs, and thus, the mechanism remains unclear. We find that spores of the yeast Saccharomyces cerevisiae are internalized efficiently by NPPs. Analyses of this phenomenon reveals that RNA fragments derived from cytosolic RNA species are attached to the spore wall, and these fragments serve as ligands to induce spore internalization. Furthermore, we show that a multiligand receptor, RAGE (receptor for advanced glycation end-products), mediates phagocytosis in NPPs. RAGE-mediated phagocytosis is not uniquely induced by spores but is an intrinsic mechanism by which NPPs internalize macromolecules containing RAGE ligands. In fact, artificial particles labeled with polynucleotides, HMGB1, or histone (but not bovine serum albumin) are internalized in NPPs. Our findings provide insight into the molecular basis of phagocytosis by NPPs, a process by which a variety of macromolecules are targeted for internalization.

      DOI: 10.1038/s42003-022-03791-1

      PubMed

      researchmap

    • Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension. International journal

      Tsuyoshi S Nakamura, Yasuyuki Suda, Kenji Muneshige, Yuji Fujieda, Yuuya Okumura, Ichiro Inoue, Takayuki Tanaka, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Yasushi Okada, Aaron M Neiman, Hiroyuki Tachikawa

      PLoS genetics17 ( 8 ) e1009727   8 2021

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      Vps13 family proteins are proposed to function in bulk lipid transfer between membranes, but little is known about their regulation. During sporulation of Saccharomyces cerevisiae, Vps13 localizes to the prospore membrane (PSM) via the Spo71-Spo73 adaptor complex. We previously reported that loss of any of these proteins causes PSM extension and subsequent sporulation defects, yet their precise function remains unclear. Here, we performed a genetic screen and identified genes coding for a fragment of phosphatidylinositol (PI) 4-kinase catalytic subunit and PI 4-kinase noncatalytic subunit as multicopy suppressors of spo73Δ. Further genetic and cytological analyses revealed that lowering PI4P levels in the PSM rescues the spo73Δ defects. Furthermore, overexpression of VPS13 and lowering PI4P levels synergistically rescued the defect of a spo71Δ spo73Δ double mutant, suggesting that PI4P might regulate Vps13 function. In addition, we show that an N-terminal fragment of Vps13 has affinity for the endoplasmic reticulum (ER), and ER-plasma membrane (PM) tethers localize along the PSM in a manner dependent on Vps13 and the adaptor complex. These observations suggest that Vps13 and the adaptor complex recruit ER-PM tethers to ER-PSM contact sites. Our analysis revealed that involvement of a phosphoinositide, PI4P, in regulation of Vps13, and also suggest that distinct contact site proteins function cooperatively to promote de novo membrane formation.

      DOI: 10.1371/journal.pgen.1009727

      PubMed

      researchmap

    • Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT.

      Feng Li, Ganglong Yang, Hiroyuki Tachikawa, Kankai Shao, Yan Yang, Xiao-Dong Gao, Hideki Nakanishi

      The Journal of general and applied microbiology67 ( 1 ) 33 - 41   16 4 2021

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      O-GlcNAc modification mediated by O-GlcNAc transferase (OGT) is a reversible protein modification in which O-GlcNAc moieties are attached to target proteins in the cytosol, nucleus, and mitochondria. O-GlcNAc moieties attached to proteins can be removed by O-GlcNAcase (OGA). The addition of an O-GlcNAc moiety can influence several aspects of protein function, and aberrant O-GlcNAc modification is linked to a number of diseases. While OGT and OGA are conserved across eukaryotic cells, yeasts lack these enzymes. Previously, we reported that protein O-GlcNAc modification occurred in the budding yeast Saccharomyces cerevisiae when OGT was ectopically expressed. Because yeast cells lack OGA, O-GlcNAc moieties are stably attached to target proteins. Thus, the yeast system may be useful for finding novel OST substrates. By proteomic analysis, we identified 468 O-GlcNAcylated proteins in yeast cells expressing human OGT. Among these proteins, 13 have human orthologues that show more than 30% identity to their corresponding yeast orthologue, and possible glycosylation residues are conserved in these human orthologues. In addition, the orthologues have not been reported as substrates of OGT. We verified that some of these human orthologues are O-GlcNAcylated in cultured human cells. These proteins include an ubiquitin-conjugating enzyme, UBE2D1, and an eRF3-similar protein, HBS1L. Thus, the yeast system would be useful to find previously unknown O-GlcNAcylated proteins and regulatory mechanisms.

      DOI: 10.2323/jgam.2020.04.002

      PubMed

      researchmap

    • LYAR potentiates rRNA synthesis by recruting BRD2/4 and MYST-type acetyltransferase KAT7 to rDNA Peer-reviewed International journal

      Izumikawa K, Ishikawa H, Yoshikawa H, Fujiyama S, Watanabe A, Aburatani H, Tachikawa H, Hayano T, Isobe T, Simpson R, Li L, Min J, Takahashi N

      Nucleic Acids Research47 ( 19 ) 10357 - 10372   11 2019

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      Activation of ribosomal RNA (rRNA) synthesis is pivotal during cell growth and proliferation, but its aberrant upregulation may promote tumorigenesis. Here, we demonstrate that the candidate oncoprotein, LYAR, enhances ribosomal DNA (rDNA) transcription. Our data reveal that LYAR binds the histone-associated protein BRD2 without involvement of acetyl-lysine-binding bromodomains and recruits BRD2 to the rDNA promoter and transcribed regions via association with upstream binding factor. We show that BRD2 is required for the recruitment of the MYST-type acetyltransferase KAT7 to rDNA loci, resulting in enhanced local acetylation of histone H4. In addition, LYAR binds a complex of BRD4 and KAT7, which is then recruited to rDNA independently of the BRD2-KAT7 complex to accelerate the local acetylation of both H4 and H3. BRD2 also helps recruit BRD4 to rDNA. By contrast, LYAR has no effect on rDNA methylation or the binding of RNA polymerase I subunits to rDNA. These data suggest that LYAR promotes the association of the BRD2-KAT7 and BRD4-KAT7 complexes with transcription-competent rDNA loci but not to transcriptionally silent rDNA loci, thereby increasing rRNA synthesis by altering the local acetylation status of histone H3 and H4.

      DOI: 10.1093/nar/gkz747

      PubMed

      researchmap

    • Osw2 is required for proper assembly of glucan and/or mannan layers of the yeast spore wall Peer-reviewed

      Hua-Ping Pan, Ning Wang, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi

      Journal of Biochemistry163 ( 4 ) 293 - 304   1 4 2018

      More details

      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/jb/mvx082

      Scopus

      PubMed

      researchmap

    • Activation of Rab GTPase Sec4 by its GEF Sec2 is required for prospore membrane formation during sporulation in yeast Saccharomyces cerevisiae. Peer-reviewed

      Suda Y, Tachikawa H, Inoue I, Kurita T, Saito C, Kurokawa K, Nakano A, Irie K

      FEMS Yeast Research18 ( 1 )   2 2018

      More details

      Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

      DOI: 10.1093/femsyr/fox095

      PubMed

      researchmap

    • Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation Peer-reviewed

      Tsuyoshi S. Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M. Neiman, Hiroyuki Tachikawa

      MOLECULAR BIOLOGY OF THE CELL28 ( 26 ) 3881 - 3895   12 2017

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1091/mbc.E17-08-0521

      PubMed

      researchmap

    • beta-1,6-glucan synthesis-associated genes are required for proper spore wall formation in Saccharomyces cerevisiae Peer-reviewed

      Hua-Ping Pan, Ning Wang, Hiroyuki Tachikawa, Hideki Nakanishi, Xiao-Dong Gao

      YEAST34 ( 11 ) 431 - 446   11 2017

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1002/yea.3244

      PubMed

      researchmap

    • Identification and characterization of transcriptional control region of the human beta 1,4-mannosyltransferase gene Peer-reviewed

      Tetsuo Takahashi, Takashi Nedachi, Takuya Etoh, Hiroyuki Tachikawa, Xiao-Dong Gao

      CYTOTECHNOLOGY69 ( 3 ) 417 - 434   6 2017

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1007/s10616-015-9929-y

      PubMed

      researchmap

    • The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae Peer-reviewed

      Yuuya Okumura, Tsuyoshi S. Nakamura, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Shugo Nakamura, Xiao-Dong Gao, Hiroyuki Tachikawa

      MSPHERE1 ( 1 )   1 2016

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/mSphere.00038-15

      PubMed

      researchmap

    • Yeast cell-based analysis of human lactate dehydrogenase isoforms Peer-reviewed

      Lulu Ahmed Mohamed, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi

      JOURNAL OF BIOCHEMISTRY158 ( 6 ) 467 - 476   12 2015

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1093/jb/mvv061

      PubMed

      researchmap

    • Use of Yeast Spores for Microencapsulation of Enzymes Peer-reviewed

      Libing Shi, Zijie Li, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY80 ( 15 ) 4502 - 4510   8 2014

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/AEM.00153-14

      PubMed

      researchmap

    • Applied Usage of Yeast Spores as Chitosan Beads Peer-reviewed

      Haini Zhang, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi

      APPLIED AND ENVIRONMENTAL MICROBIOLOGY80 ( 16 ) 5098 - 5105   8 2014

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/AEM.00677-14

      PubMed

      researchmap

    • Natural IgG antibody with anti-beta-galactosyl specificity suppressed hepatoma cell invasion in culture Peer-reviewed

      Yutaka Miura, Hiroshi Fujita, Fumihiko Sakai, Hiroyuki Tachikawa, Kazumi Yagasaki, Daisaburo Fujimoto

      CYTOTECHNOLOGY65 ( 6 ) 909 - 913   12 2013

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1007/s10616-012-9523-5

      PubMed

      researchmap

    • SPO71 encodes a developmental stage-specific partner for Vps13 in Saccharomyces cerevisiae Peer-reviewed

      Jae-Sook Park, Yuuya Okumura, Hiroyuki Tachikawa, Aaron M. Neiman

      Eukaryotic Cell12 ( 11 ) 1530 - 1537   11 2013

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/EC.00239-13

      Scopus

      PubMed

      researchmap

    • Alg14 organizes the formation of a multiglycosyltransferase complex involved in initiation of lipid-linked oligosaccharide biosynthesis Peer-reviewed

      Jishun Lu, Tetsuo Takahashi, Atsuko Ohoka, Kei-ichi Nakajima, Ryo Hashimoto, Nobuaki Miura, Hiroyuki Tachikawa, Xiao-Dong Gao

      GLYCOBIOLOGY22 ( 4 ) 504 - 516   4 2012

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1093/glycob/cwr162

      PubMed

      researchmap

    • Splicing Factor 2-Associated Protein p32 Participates in Ribosome Biogenesis by Regulating the Binding of Nop52 and Fibrillarin to Preribosome Particles Peer-reviewed

      Harunori Yoshikawa, Wataru Komatsu, Toshiya Hayano, Yutaka Miura, Keiichi Homma, Keiichi Izumikawa, Hideaki Ishikawa, Naoki Miyazawa, Hiroyuki Tachikawa, Yoshio Yamauchi, Toshiaki Isobe, Nobuhiro Takahashi

      MOLECULAR & CELLULAR PROTEOMICS10 ( 8 ) M110.006148   8 2011

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1074/mcp.M110.006148

      PubMed

      researchmap

    • Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast Peer-reviewed

      Onishi, M., Koga, T., Hirata, A., Nakamura, T., Asakawa, H., Shimoda, C., Bähler, J., Wu, J.-Q., Takegawa, K., Tachikawa, H., Pringle, J.R., Fukui, Y.

      Molecular and Cellular Biology30 ( 8 ) 2057 - 2074   2010

      More details

      Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/MCB.01529-09

      Scopus

      PubMed

      researchmap

    • Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation Peer-reviewed

      Makoto Ishihara, Yasuyuki Suda, Ichiro Inoue, Takayuki Tanaka, Tetsuo Takahashi, Xiao-Dong Gao, Yasuhisa Fukui, Sayoko Ihara, Aaron M. Neiman, Hiroyuki Tachikawa

      EUKARYOTIC CELL8 ( 7 ) 1027 - 1037   7 2009

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/EC.00095-09

      PubMed

      researchmap

    • The Anaphase Promoting Complex Targeting Subunit Ama1 Links Meiotic Exit to Cytokinesis during Sporulation in Saccharomyces cerevisiae Peer-reviewed

      Aviva E. Diamond, Jae-Sook Park, Ichiro Inoue, Hiroyuki Tachikawa, Aaron M. Neiman

      MOLECULAR BIOLOGY OF THE CELL20 ( 1 ) 134 - 145   1 2009

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1091/mbc.E08-06-0615

      PubMed

      researchmap

    • Septins localize to microtubules during nutritional limitation in Saccharomyces cerevisiae Peer-reviewed

      M. Evangelina Pablo-Hernando, Yolanda Arnaiz-Pita, Hiroyuki Tachikawa, Francisco del Rey, Aaron M. Neiman, Carlos R. Vazquez de Aldana

      BMC CELL BIOLOGY9   55   10 2008

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1186/1471-2121-9-55

      PubMed

      researchmap

    • Association of human DNA helicase RecQ5 beta with RNA polymerase II and its possible role in transcription Peer-reviewed

      Keiichi Izumikawa, Mitsuaki Yanagida, Toshiya Hayano, Hiroyuki Tachikawa, Wataru Komatsu, Akira Shimamoto, Kazunobu Futami, Yasuhiro Furuichi, Takashi Shinkawa, Yoshio Yamauchi, Toshiaki Isobe, Nobuhiro Takahashi

      BIOCHEMICAL JOURNAL413 ( 3 ) 505 - 516   8 2008

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1042/BJ20071392

      PubMed

      researchmap

    • Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation Peer-reviewed

      Masayuki Onishi, Michihiro Iida, Takako Koga, Sadayuki Yamada, Aiko Hirata, Tomoko Iwaki, Kaoru Takegawa, Yasuhisa Fukui, Hiroyuki Tachikawa

      EUKARYOTIC CELL6 ( 12 ) 2343 - 2353   12 2007

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1128/EC.00211-07

      PubMed

      researchmap

    • Pleckstrin-2 selectively interacts with phosphatidylinositol 3-kinase lipid products and regulates actin organization and cell spreading Peer-reviewed

      Norihisa Hamaguchi, Sayoko Ihara, Tsutomu Ohdaira, Hiromichi Nagano, Akihiro Iwamatsu, Hiroyuki Tachikawa, Yasuhisa Fukui

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS361 ( 2 ) 270 - 275   9 2007

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/j.bbrc.2007.06.132

      PubMed

      researchmap

    • SWAP-70 is required for oncogenic transformation by v-Src in mouse embryo fibroblasts Peer-reviewed

      Yasuhisa Fukui, Takayuki Tanaka, Hiroyuki Tachikawa, Sayoko Ihara

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS356 ( 2 ) 512 - 516   5 2007

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/j.bbrc.2007.03.011

      PubMed

      researchmap

    • Muc4 is required for activation of ErbB2 in signet ring carcinoma cell lines Peer-reviewed

      Atsushi Yokoyama, Bin-Hai Shi, Takayuki Kawai, Hiroaki Konishi, Ryota Andoh, Hiroyuki Tachikawa, Sayoko Ihara, Yasuhisa Fukui

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS355 ( 1 ) 200 - 203   3 2007

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/j.bbrc.2007.01.133

      PubMed

      researchmap

    • Activity of beta 3-beta 4 loop of the PH domain is required for the membrane targeting of SWAP-70 Peer-reviewed

      Yasuhisa Fukui, Isamu Wakamatsu, Hiroyuki Tachikawa, Yoshihiko Okamura, Takayuki Tanaka, Sayoko Ihara

      IUBMB LIFE59 ( 2 ) 99 - 103   2 2007

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1080/15216540701222880

      PubMed

      researchmap

    • TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system Peer-reviewed

      Hideaki Ishikawa, Hiroyuki Tachikawa, Yutaka Miura, Nobuhiro Takahashi

      FEBS LETTERS580 ( 20 ) 4784 - 4792   9 2006

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/j.febslet.2006.07.066

      PubMed

      researchmap

    • Functional proteomic analysis of endogenous RecQ5beta-associated complex

      Izumikawa Keiichi, Yanagida Mitsuaki, Hayano Toshiya, Tachikawa Hiroyuki, Shimamoto Akira, Furuichi Yasuhiro, Shinkawa Takashi, Yamauchi Yoshio, Isobe Toshiaki, Takahashi Nobuhiro

      Abstracts for Annual Meeting of Japanese Proteomics Society2006   72 - 72   2006

      More details

      Language:Japanese   Publisher:Japanese Proteomics Society (Japan Human Proteome Organisation)  

      DOI: 10.14889/jhupo.2006.0.72.0

      CiNii Article

      researchmap

    • Alg14 recruits alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation Peer-reviewed

      XD Gao, H Tachikawa, T Sato, Y Jigami, N Dean

      JOURNAL OF BIOLOGICAL CHEMISTRY280 ( 43 ) 36254 - 36262   10 2005

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1074/jbc.M507569200

      PubMed

      researchmap

    • Calcineurin inhibitors block dorsal-side signaling that affect late-stage development of the heart, kidney, liver, gut and somitic tissue during Xenopus embryogenesis Peer-reviewed

      Y Yoshida, S Kim, K Chiba, S Kawai, H Tachikawa, N Takahashi

      DEVELOPMENT GROWTH & DIFFERENTIATION46 ( 2 ) 139 - 152   4 2004

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1111/j.1440-169X.2004.00733.x

      PubMed

      researchmap

    • Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals Peer-reviewed

      Y Suda, H Tachikawa, A Yokota, H Nakanishi, N Yamashita, Y Miura, N Takahashi

      YEAST20 ( 11 ) 995 - 1005   8 2003

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1002/yea.1008

      PubMed

      researchmap

    • A Gip1p-Glc7p phosphatase complex regulates septin organization and spore wall formation Peer-reviewed

      H Tachikawa, A Bloecher, K Tatchell, AM Neiman

      JOURNAL OF CELL BIOLOGY155 ( 5 ) 797 - 808   11 2001

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1083/jcb.200107008

      researchmap

    • Hut1 proteins identified in Saccharomyces cerevisiae and Schizosaccharomyces pombe are functional homologues involved in the protein-folding process at the endoplasmic reticulum Peer-reviewed

      H Nakanishi, K Nakayama, A Yokota, H Tachikawa, N Takahashi, Y Jigami

      YEAST18 ( 6 ) 543 - 554   4 2001

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1002/yea.707

      researchmap

    • Characterization of the subpopulations of rat ascites hepatoma AH109A cells with different invasive and metastatic activities Peer-reviewed

      Y Miura, M Miyauchi, K Kubota, Q Li, H Tachikawa, D Fujimoto, K Yagasaki

      ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 10   205 - 209   1999

      More details

      Language:English   Publishing type:Research paper (international conference proceedings)  

      researchmap

    • Molecular cloning and characterization of mouse ficolin-A Peer-reviewed

      Y Fujimori, S Harumiya, Y Fukumoto, Y Miura, K Yagasaki, H Tachikawa, D Fujimoto

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS244 ( 3 ) 796 - 800   3 1998

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1006/bbrc.1998.8344

      researchmap

    • Overproduction of Mpd2p suppresses the lethality of protein disulfide isomerase depletion in a CXXC sequence dependent manner Peer-reviewed

      H Tachikawa, W Funahashi, Y Takeuchi, H Nakanishi, R Nishihara, S Katoh, XD Gao, T Mizunaga, D Fujimoto

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS239 ( 3 ) 710 - 714   10 1997

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1006/bbrc.1997.7426

      researchmap

    • Similarity between galectin-5 and rkCBP17.5 Peer-reviewed

      E Kondo, S Yokote, R Miyamoto, Y Miyake, H Kagawa, S Harumiya, M Totsuka, H Tachikawa, S Kaminogawa, D Fujimoto

      BIOMEDICAL RESEARCH-TOKYO17 ( 6 ) 491 - 494   12 1996

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.2220/biomedres.17.491

      researchmap

    • Characterization of ficolins as novel elastin-binding proteins and molecular cloning of human ficolin-1 Peer-reviewed

      S Harumiya, K Takeda, T Sugiura, Y Fukumoto, H Tachikawa, K Miyazono, D Fujimoto, H Ichijo

      JOURNAL OF BIOCHEMISTRY120 ( 4 ) 745 - 751   10 1996

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1093/oxfordjournals.jbchem.a021474

      researchmap

    • A novel β-galactoside-binding lectin in cultured murine lymphocytic leukemia cells Peer-reviewed

      Angela Mai, Sang-Kee Jung, Hiroyuki Tachikawa, Daisaburo Fujimoto

      Journal of Biochemistry120 ( 3 ) 478 - 480   1996

      More details

      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/oxfordjournals.jbchem.a021436

      Scopus

      PubMed

      researchmap

    • ISOLATION AND CHARACTERIZATION OF A YEAST GENE, MPD1, THE OVEREXPRESSION OF WHICH SUPPRESSES INVIABILITY CAUSED BY PROTEIN DISULFIDE-ISOMERASE DEPLETION Peer-reviewed

      H TACHIKAWA, Y TAKEUCHI, W FUNAHASHI, T MIURA, XD GAO, D FUJIMOTO, T MIZUNAGA, K ONODERA

      FEBS LETTERS369 ( 2-3 ) 212 - 216   8 1995

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/0014-5793(95)00750-4

      researchmap

    • EBP-37, A NEW ELASTIN BINDING-PROTEIN IN HUMAN PLASMA - STRUCTURAL SIMILARITY TO FICOLINS, TRANSFORMING GROWTH-FACTOR-BETA-1 BINDING-PROTEINS Peer-reviewed

      S HARUMIYA, A OMORI, T SUGIURA, Y FUKUMOTO, H TACHIKAWA, D FUJIMOTO

      JOURNAL OF BIOCHEMISTRY117 ( 5 ) 1029 - 1035   5 1995

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1093/oxfordjournals.jbchem.a124802

      researchmap

    • CHARACTERIZATION OF NATURAL IGG ANTIBODY WITH ANTI-BETA-GALACTOSYL SPECIFICITY Peer-reviewed

      H FUJITA, SK JUNG, Y MUKAINAKA, H TACHIKAWA, D FUJIMOTO, T KOSUGE, T SHIRAI, A TAKEIRI, Y SUZUKI

      BIOMEDICAL RESEARCH-TOKYO15 ( 1 ) 17 - 25   2 1994

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.2220/biomedres.15.17

      researchmap

    • MOLECULAR-STRUCTURE OF A YEAST GENE, PDI1, ENCODING PROTEIN DISULFIDE ISOMERASE THAT IS ESSENTIAL FOR CELL-GROWTH Peer-reviewed

      H TACHIKAWA, T MIURA, Y KATAKURA, T MIZUNAGA

      JOURNAL OF BIOCHEMISTRY110 ( 2 ) 306 - 313   8 1991

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1093/oxfordjournals.jbchem.a123576

      researchmap

    • GLYCOSYLATION SITE BINDING-PROTEIN AND PROTEIN DISULFIDE ISOMERASE ARE IDENTICAL AND ESSENTIAL FOR CELL VIABILITY IN YEAST Peer-reviewed

      M LAMANTIA, T MIURA, H TACHIKAWA, HA KAPLAN, WJ LENNARZ, T MIZUNAGA

      PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA88 ( 10 ) 4453 - 4457   5 1991

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1073/pnas.88.10.4453

      researchmap

    ▼display all

    Misc.

    • Involvement of p32, fibrillarin, and Nop52 in pre-90S particle separation during human ribosome biogenesis

      H. Yoshikawa, W. Komatsu, T. Hayano, Y. Miura, K. Homma, K. Izumikawa, H. Ishikawa, N. Miyazawa, H. Tachikawa, Y. Yamauchi, T. Isobe, N. Takahashi

      MOLECULAR BIOLOGY OF THE CELL22   2011

      More details

      Language:English   Publishing type:Research paper, summary (international conference)  

      researchmap

    • 出芽酵母の前胞子膜伸長に必要なPP1複合体の機能解析

      大平孝博, 田中貴之, 福地栄太, 須田恭之, 高橋哲夫, 伊原さよ子, 舘川宏之

      日本農芸化学会大会講演要旨集2010   2010

      More details

    • 出芽酵母胞子形成に必須なPP1ターゲティングサブユニットであるGip1の解析

      田中貴之, 福地栄太, 高橋哲夫, 伊原さよ子, 舘川宏之

      日本農芸化学会大会講演要旨集2009   2009

      More details

    • Regulation of the ARC105-mediated transcription by TRIM11

      H. Ishikawa, H. Tachikawa, Y. Miura, N. Takahashi

      FEBS JOURNAL274   218 - 218   7 2007

      More details

      Language:English   Publishing type:Research paper, summary (international conference)  

      researchmap

    • Proteomic analysis of RecQ5 beta-associated protein complex that may constitute a part of transcriptional machinery

      K Izumikawa, M Yanagida, T Hayano, H Tachikawa, A Shimamoto, Y Furuichi, T Isobe, N Takahashi

      FASEB JOURNAL19 ( 4 ) A873 - A873   3 2005

      More details

      Language:English   Publishing type:Research paper, summary (international conference)  

      researchmap

    • DNA helicase RecQ5 βの機能解析

      泉川桂一, 柳田光昭, 嶋本顕, 舘川宏之, 磯辺俊明, 古市泰宏, 高橋信弘

      日本分子生物学会年会プログラム・講演要旨集27th   2004

      More details

    • Possible involvement of human parvulin, a peptidyl-prolyl isomerase, in early stages of ribosome biogenesis in multi-cellular organisms

      S Fujiyama, M Yanagida, T Hayano, Y Miura, H Tachikawa, T Isobe, N Takahashi

      FASEB JOURNAL17 ( 5 ) A1307 - A1307   3 2003

      More details

      Language:English   Publishing type:Research paper, summary (international conference)  

      researchmap

    • Characterization of EBP-37 and ficolins as novel elastin-binding proteins in human and porcine plasmas and molecular cloning of human ficolin-1

      S Harumiya, H Ichijo, K Takeda, T Sugiura, Y Fukumoto, H Tachikawa, K Miyazono, D Fujimoto

      MATRIX BIOLOGY16 ( 2 ) 71 - 71   5 1997

      More details

      Language:English   Publishing type:Research paper, summary (international conference)  

      researchmap

    ▼display all

    Presentations

    • The mechanism by which budding yeast produces spore membranes and walls Invited

      HIROYUKI TACHIKAWA

      16 11 2023 

      More details

      Event date: 16 11 2023 - 17 11 2023

      Language:Japanese   Presentation type:Oral presentation (invited, special)  

      researchmap

    • メンブレンコンタクトサイトの再配置による脂質輸送と膜伸長の分子機構解明

      舘川宏之, 須田恭之

      第3回オルガネラ・ゾーン研究会  26 11 2019 

      More details

      Event date: 26 11 2019 - 27 11 2019

      Presentation type:Symposium, workshop panel (public)  

      researchmap

    • 酵母の前胞子膜形成におけるリン脂質とオルガネラ接触部位の役割

      舘川 宏之

      日本農芸化学会2019年東京大会  26 3 2019 

      More details

      Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

      researchmap

    • 出芽酵母前胞子膜形成の研究−新しい膜構造を構築する分子メカニズムの理解を目指して− Invited

      舘川 宏之

      酵母細胞研究会  13 7 2018 

      More details

      Language:Japanese   Presentation type:Oral presentation (invited, special)  

      researchmap

    Teaching Experience

    • 2 2023 
      Special Lecture for Microbial Symbiosis ( Tokyo University of Agriculture )

      More details

    •  
      応用生命科学特論 ( 東京農工大学 )

      More details

    •  
      細胞機能調節学 ( 宇都宮大学 )

      More details

    •  
      細胞調節生化学 ( 東京大学 )

      More details

    •  
      微生物バイオテクノロジー ( 東京大学 )

      More details

    •  
      化学と生物 基礎 ( 東京大学 )

      More details

    •  
      基礎生物化学 ( 東京大学 )

      More details

    •  
      代謝生化学 ( 東京大学 )

      More details

    •  
      細胞生物学 ( 東京大学 )

      More details

    •  
      生物化学 ( 東京大学 )

      More details

    ▼display all

    Professional Memberships

    •  
      American Society for Cell Biology

      More details

    •  
      American Society for Microbiology

      More details

    •  
      JAPAN SOCIETY FOR CELL BIOLOGY

      More details

    •  
      JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

      More details

    •  
      酵母細胞研究会

      More details

    •  
      酵母遺伝学フォーラム

      More details

    ▼display all

    Research Projects

    • Applied research to achieve national well-being, focusing on preventing periodontal disease using domestically produced sweet potatoes

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      More details

      4 2024 - 3 2027

      Grant number:24K13055

      Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

      researchmap

    • Molecular Mechanisms of Membrane Lipid Supply to the Prospore Membrane of Budding Yeast

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      More details

      4 2023 - 3 2026

      Grant number:23K05006

      Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

      researchmap

    • 出芽酵母前胞子膜形態形成の分子機構解明とその応用

      日本学術振興会  科学研究費助成事業 

      舘川 宏之

      More details

      4 2020 - 3 2023

      Grant number:20K05782

      Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

      PP1複合体とSSV複合体の前胞子膜伸長における役割を明らかにするため以下の実験を行った。
      1 PP1複合体について、野生株とgip1破壊株でリン酸化ペプチドを濃縮してMS解析を行った。関係するタンパク質が複数得られたが、3連で行ったところ再現性よく違いの見られるペプチドが得られず、さらなる工夫と解析が必要であることがわかった。
      2 昨年度、Spo71のPxPモチーフを含むドメインと前胞子膜マーカーの融合タンパク質が、Vps13を前胞子膜にリクルートできるが、それだけではspo71破壊株の胞子形成を回復できないことを示し、Spo71にはVps13を前胞子膜にリクルートする以外にも役割があることを示した。そこで、この融合タンパク質に加えて、前胞子膜のPI4Pレベルを低下させるタンパク質を同時に発現させる実験を行い、spo71破壊株の胞子形成が部分的に回復することを明らかにした。これにより、Spo71はVps13を前胞子膜にリクルートするのに加えて、前胞子膜上のPI4Pのレベルの制御に関与することが示された。
      3 SSV複合体とともに働くと考えられるtetherタンパク質(Ist2, Scs2, Scs22, Tcb1-3)の遺伝子について、多重破壊株を作製しその表現型を調べた。6重破壊株は胞子形成をしたが、前胞子膜の形態、そして胞子の形態が異常になることを明らかにした。このことは、tetherタンパク質が前胞子膜の形態形成に関係することを示している。さらにICE2を加えて7重破壊株を作製したが、不安定な表現型を示したので、再度検討の必要がある。

      researchmap

    • Molecular mechanism of prospore membrane extension in budding yeast: protein dephosphorylation and regulation of phospholipid metabolism

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      Tachikawa Hiroyuki

      More details

      4 2017 - 3 2020

      Grant number:17K07712

      Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

      Sporulation of budding yeast is a dynamic process of intracellular reorganization. In this study, to know the mechanism of de novo membrane formation in the cell, we analyzed the formation of the prospore membrane which appears during spore formation. As to the protein phosphatase type I complex which functions in this process, we tried to identify target protein and obtained some candidates. As to Vps13 containing complex, we showed that it forms the ER-prospore membrane contact sites with other contact site proteins, and functions in regulation of the levels of phospholipids and prospore membrane extension. This study contributed to our understanding of function of Vps13 in membrane formation.

      researchmap

    • Elucidation of the role of phospholipid metabolism in the prospore membrane formation of budding yeast

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      TACHIKAWA HIROYUKI

      More details

      4 2013 - 3 2016

      Grant number:25450094

      Grant amount:\5200000 ( Direct Cost: \4000000 、 Indirect Cost:\1200000 )

      Prospore membrane formation of Saccharomyces cerevisiae is a de novo membrane formation in side the cell to the proper size and proper shape and is a good model for membrane morphogenesis. We and other groups have been analyzing the genes involved in this process. Our genetic screen revealed that Phosphatidylinositol (PI) 4-kinase complex is involved in the prospore membrane formation. We also showed that PI4P and PI4-kinase complex together with Phosphatidic Acid and Phosphatidylserine are on the prospore membrane. Further, our analyses suggest that decrease of PI4P on the prospore membrane is related to extension of the prospore membrane, and that regulation of PI4P levels on the prospore membrane is important for its extension. Our result will contribute to the understanding of the role of PI4P in membrane formation in the cell.

      researchmap

    • Elucidation of the molecular mechanism underlying the prospore membrane formation in Saccharomyces cerevisiae and its application

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      TACHIKAWA Hiroyuki

      More details

      2010 - 2012

      Grant number:22580076

      Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

      Prospore membrane formation of budding yeast is studied as a model system for membrane biogenesis. In this study, we performed domain analysis on protein phosphatase type I targeting subunit, Gip1, and showed that distinct domains are required for its localization and function. We also showed that Spo71 and Spo73, peripheral membrane proteins required for prospore membrane formation, interact together on prospore membrane. Spo73 is a dysferlin domain-only protein. Our analysis on Spo73 provides some clues to elucidate the mechanism of a type of muscular dystrophy caused by mutations in dysferlin domain of dysferlin.

      researchmap

    • Focused proteomic analysis of functional network for peptidyl prolyl cis/trans isomerases

      Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research 

      TAKAHASHI Nobuhiro, TACHIKAWA Hiroyuki

      More details

      2003 - 2005

      Grant number:15310139

      Grant amount:\13300000 ( Direct Cost: \13300000 )

      Peptidyl prolyl cis/trans isomerases (PPlases) catalyze the rotation about the peptide bond preceding proline, a step that can be rate-limiting for the folding of newly synthesized proteins. PPlases also have the ability to bind many proteins and to act as chaperones; thus they are believed to regulate folding, assembly and trafficking, and controlling activity of proteins in the cell. PPlases are most familiar as the targets of the immunosuppressive drugs, cyclosporin A (CsA) and FK506, which bind, respectively, to cyclophilin (CyP) and FK506-binding protein (FKBP) and inhibit their cognate PPlaseactivities. Both CyP and FKBP are ubiquitous, highly expressed and conserved from bacteria to human. The third family of PPlases, parvulins, is the target of neither CsA nor FK506, but is also conserved from bacteria to human. Although a number of CyP, FKBP and parvulin homologs have been identified in almost all organisms, the cellular functions of most of those homologs remain to be exploded. In this study, we analyzed systematically potential substrate for 23 different human PPlases including 9 CyP, 11 FKBP, and 3 parvulin homologs by using proteomic approaches that include expression of epitope-tagged PPlase, affinity purification in the presence or absence of PPlase inhibitor, mass-spectrometry based protein identification, and database searching. We identified about 200 potential substrates for the three types of PPlases and constructed a functional protein network for the PPlases. The network included proteins that have roles in translation, cell cycle, proliferation, transcription, stress-responses, DNA/protein metabolism, RNA processing, protein trafficking, and ribosome biogenesis. We investigated further the involvement of PPlases in ribosome biogenesis, and found that parvulin 14, FKBP25, and CyPB have probably roles in different stages of human ribosome biogenesis.

      researchmap

    • 酵母の分泌タンパク質高次構造形成過程に関与する因子の解析

      日本学術振興会  科学研究費助成事業 

      舘川 宏之

      More details

      1996 - 1996

      Grant number:08760071

      Grant amount:\1000000 ( Direct Cost: \1000000 )

      我々は、これまで酵母PDIに関する研究を行い、PDIが生育に必須であることを示した。また、ガラクトース依存性PDI変異株を作製し、PDIが分泌タンパク質の立体構造形成に重要な役割を果たしていることを示した。そして、作製した変異株の生育を多コピーで相補する遺伝子(マルチコピーサプレッサー遺伝子)のクローニングを行い、2つの新規遺伝子(MPD1,MPD2)を取得し、その構造を明らかにした。
      本研究では、Mpd1pとMpd2pの機能および関係を調べた。まず、グルタチオンS-トランスフェラーゼとの融合タンパク質として、大腸菌内で発現させることにより、Mpd1pがPDI活性を持つタンパク質であることを示した。Mpd2pは、融合タンパク質が不溶性となったため、今後検討が必要である。また、Mpd1pおよびMpd2pの活性中心様配列にin vitro mutagenesis法により変異を導入した結果、この領域がPDIの欠損のマルチコピーサプレッサーとして働く為には必須であることが明らかになった。これらより、Mpd1pとMpd2pはPDIと同様な機能を持つタンパク質であり、これらがPDIのかわりをすることによりPDIの欠損を相補することが示唆された。また、遺伝学的関係を知るため、MPD1,MPD2,EUG1遺伝子の三重破壊体酵母を作製したが、生育可能で、顕著な表現形は観察されなかった。
      今後は、PDI,Mpd1p,Mpd2p,Eug1pの基質特異性の違いや発現の違いを観察することにより、これらタンパク質の生体内における役割を明らかにしていく予定である。

      researchmap

    ▼display all