Updated on 2025/10/09

写真b

 
HANADA Yuki
 
*Items subject to periodic update by Rikkyo University (The rest are reprinted from information registered on researchmap.)
Affiliation*
College of Science Department of Life Science
Title*
Assistant Professor
Degree
Doctor of Medical Science ( 3 2021   Kyushu University )
Research Interests
  • Molecular cell biology at the surface of organelle membranes

  • Membrane protein

  • Mff

  • Energy sensing

  • Mitochondrial fission/fusion

  • Metabolism

  • Immune response

  • Mitochondria

  • Campus Career*
    • 4 2023 - Present 
      College of Science   Department of Life Science   Assistant Professor
    Profile

    My research focuses on the molecular mechanisms of the mitochondria-mediated link between metabolism and immunity, the significance of the evolutionarily acquired mitochondrial function, and its regulation. In particular, I am interested in the function of the mitochondrial outer membrane in response to changes in intracellular energy status. In the future, I hope to elucidate the physiological role of regulatory factors in mitochondrial morphology and function from the perspective of nutrition and metabolism.

     

    Research Areas

    • Life Science / Cell biology

    • Life Science / Metabolism and endocrinology

    Research History

    • 4 2023 - Present 
      Rikkyo University   Department of Life Science   Assistant professor

      More details

      Notes:Laboratory of Molecular Cell Biology

      researchmap

    • 4 2019 - 3 2023 
      Osaka University   Graduate School of Science Department of Biological Sciences   Researcher

      More details

      Notes:Laboratory of Cellular Life Science

      researchmap

    Education

    • 5 2018 - 3 2020 
      Osaka University   Graduate School of Science   Department of Biological Sciences

      More details

    • 4 2015 - 3 2020 
      Kyushu University   Faculty of Medical Sciences

      More details

    • 4 2009 - 3 2015 
      Kyushu University   School of Pharmaceutical Sciences

      More details

    Awards

    • 9 2018  
      the 91st Annual Meeting of the Japanese Biochemical Society  Young Scientist Award 

      More details

      Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

      researchmap

    Papers

    • Alternative splicing of Mff regulates AMPK-mediated phosphorylation, mitochondrial fission and antiviral response Invited Peer-reviewed

      Yuki Hanada, Risa Maeda, Takaya Ishihara, Masaki Nakahashi, Yuichi Matsushima, Emi Ogasawara, Toshihiko Oka, Naotada Ishihara

      Pharmacological Research209   107414   16 9 2024

      More details

      Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

      Mitochondrial morphology and function change dynamically in response to intracellular signaling and the surrounding environment. The mitochondrial fission factor Mff, which localizes to the outer mitochondrial membrane, mediates not only mitochondrial fission by recruiting the dynamin-related GTPase Drp1 to mitochondrial fission sites but also the double-stranded RNA-induced antiviral response on mitochondria through mitochondrial antiviral signaling (MAVS). Mff is reported to be regulated by AMP-activated protein kinase (AMPK)-mediated protein phosphorylation and alternative pre-mRNA splicing; however, the relationships among RNA splicing, phosphorylation, and multiple functions of Mff have not been fully understood. Here, we showed that mouse Mff has a tissue-specific splicing pattern, and at least eight Mff splice isoforms were expressed in mouse embryonic fibroblasts (MEFs). We introduced single Mff isoforms into Mff knockout MEFs and found that insertion of exon 6 just after the phosphorylation site, by the alternative splicing, reduced its phosphorylation by AMPK and its functions in mitochondrial fission and the antiviral response. In addition, the underlying mechanism repressing these functions was independent of phosphorylation. These results indicate that multiple functions of Mff on mitochondria are regulated by AMPK-mediated phosphorylation and alternative splicing, under the control of energy metabolism and cellular differentiation.

      DOI: 10.1016/j.phrs.2024.107414

      researchmap

    • Non-alcoholic fatty liver disease in mice with hepatocyte-specific deletion of mitochondrial fission factor Peer-reviewed International journal

      Yukina Takeichi, Takashi Miyazawa, Shohei Sakamoto, Yuki Hanada, Lixiang Wang, Kazuhito Gotoh, Keiichiro Uchida, Shunsuke Katsuhara, Ryuichi Sakamoto, Takaya Ishihara, Keiji Masuda, Naotada Ishihara, Masatoshi Nomura, Yoshihiro Ogawa

      Diabetologia64 ( 9 ) 2092 - 2107   9 2021

      More details

      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

      Aims/hypothesis: Mitochondria are highly dynamic organelles continuously undergoing fission and fusion, referred to as mitochondrial dynamics, to adapt to nutritional demands. Evidence suggests that impaired mitochondrial dynamics leads to metabolic abnormalities such as non-alcoholic steatohepatitis (NASH) phenotypes. However, how mitochondrial dynamics are involved in the development of NASH is poorly understood. This study aimed to elucidate the role of mitochondrial fission factor (MFF) in the development of NASH.

      Methods: We created mice with hepatocyte-specific deletion of MFF (MffLiKO). MffLiKO mice fed normal chow diet (NCD) or high-fat diet (HFD) were evaluated for metabolic variables and their livers were examined by histological analysis. To elucidate the mechanism of development of NASH, we examined the expression of genes related to endoplasmic reticulum (ER) stress and lipid metabolism, and the secretion of triacylglycerol (TG) using the liver and primary hepatocytes isolated from MffLiKO and control mice.

      Results: MffLiKO mice showed aberrant mitochondrial morphologies with no obvious NASH phenotypes during NCD, while they developed full-blown NASH phenotypes in response to HFD. Expression of genes related to ER stress was markedly upregulated in the liver from MffLiKO mice. In addition, expression of genes related to hepatic TG secretion was downregulated, with reduced hepatic TG secretion in MffLiKO mice in vivo and in primary cultures of MFF-deficient hepatocytes in vitro. Furthermore, thapsigargin-induced ER stress suppressed TG secretion in primary hepatocytes isolated from control mice.

      Conclusions/interpretation: We demonstrated that ablation of MFF in liver provoked ER stress and reduced hepatic TG secretion in vivo and in vitro. Moreover, MffLiKO mice were more susceptible to HFD-induced NASH phenotype than control mice, partly because of ER stress-induced apoptosis of hepatocytes and suppression of TG secretion from hepatocytes. This study provides evidence for the role of mitochondrial fission in the development of NASH.

      DOI: 10.1007/s00125-021-05488-2

      PubMed

      researchmap

      Other Link: https://link.springer.com/article/10.1007/s00125-021-05488-2/fulltext.html

    • Dynamin-related protein 1 deficiency accelerates lipopolysaccharide-induced acute liver injury and inflammation in mice Peer-reviewed

      Lixiang Wang, Xin Li, Yuki Hanada, Nao Hasuzawa, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamoto

      Communications Biology4   894   21 7 2021

      More details

      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

      Mitochondrial fusion and fission, which are strongly related to normal mitochondrial function, are referred to as mitochondrial dynamics. Mitochondrial fusion defects in the liver cause a non-alcoholic steatohepatitis-like phenotype and liver cancer. However, whether mitochondrial fission defect directly impair liver function and stimulate liver disease progression, too, is unclear. Dynamin-related protein 1 (DRP1) is a key factor controlling mitochondrial fission. We hypothesized that DRP1 defects are a causal factor directly involved in liver disease development and stimulate liver disease progression. Drp1 defects directly promoted endoplasmic reticulum (ER) stress, hepatocyte death, and subsequently induced infiltration of inflammatory macrophages. Drp1 deletion increased the expression of numerous genes involved in the immune response and DNA damage in Drp1LiKO mouse primary hepatocytes. We administered lipopolysaccharide (LPS) to liver-specific Drp1-knockout (Drp1LiKO) mice and observed an increased inflammatory cytokine expression in the liver and serum caused by exaggerated ER stress and enhanced inflammasome activation. This study indicates that Drp1 defect-induced mitochondrial dynamics dysfunction directly regulates the fate and function of hepatocytes and enhances LPS-induced acute liver injury in vivo.

      DOI: 10.1038/s42003-021-02413-6

      researchmap

      Other Link: http://www.nature.com/articles/s42003-021-02413-6

    • MAVS is energized by Mff which senses mitochondrial metabolism via AMPK for acute antiviral immunity Peer-reviewed International journal

      Yuki Hanada, Naotada Ishihara, Lixiang Wang, Hidenori Otera, Takaya Ishihara, Takumi Koshiba, Katsuyoshi Mihara, Yoshihiro Ogawa, Masatoshi Nomura

      Nature Communications11 ( 1 ) 5711   11 11 2020

      More details

      Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

      Mitochondria are multifunctional organelles that produce energy and are critical for various signaling pathways. Mitochondrial antiviral signaling (MAVS) is a mitochondrial outer membrane protein essential for the anti-RNA viral immune response, which is regulated by mitochondrial dynamics and energetics; however, the molecular link between mitochondrial metabolism and immunity is unclear. Here we show in cultured mammalian cells that MAVS is activated by mitochondrial fission factor (Mff), which senses mitochondrial energy status. Mff mediates the formation of active MAVS clusters on mitochondria, independent of mitochondrial fission and dynamin-related protein 1. Under mitochondrial dysfunction, Mff is phosphorylated by the cellular energy sensor AMP-activated protein kinase (AMPK), leading to the disorganization of MAVS clusters and repression of the acute antiviral response. Mff also contributes to immune tolerance during chronic infection by disrupting the mitochondrial MAVS clusters. Taken together, Mff has a critical function in MAVS-mediated innate immunity, by sensing mitochondrial energy metabolism via AMPK signaling.

      DOI: 10.1038/s41467-020-19287-7

      PubMed

      researchmap

      Other Link: https://www.nature.com/articles/s41467-020-19287-7

    • Nivolumab-induced thyroid dysfunction lacking antithyroid antibody is frequently evoked in Japanese patients with malignant melanoma Peer-reviewed International journal

      Seiichi Yano, Kenji Ashida, Hiromi Nagata, Kenji Ohe, Naoko Wada, Yukina Takeichi, Yuki Hanada, Yuta Ibayashi, Lixiang Wang, Shohei Sakamoto, Ryuichi Sakamoto, Hiroshi Uchi, Motoaki Shiratsuchi, Masutaka Furue, Masatoshi Nomura, Yoshihiro Ogawa

      BMC Endocrine Disorders18 ( 36 )   8 6 2018

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1186/s12902-018-0267-x

      Scopus

      PubMed

      researchmap

      Other Link: https://bmcendocrdisord.biomedcentral.com/articles/10.1186/s12902-018-0267-x

    • Vancomycin induces reactive oxygen species-dependent apoptosis via mitochondrial cardiolipin peroxidation in renal tubular epithelial cells Peer-reviewed International journal

      Yuya Sakamoto, Takahisa Yano, Yuki Hanada, Aki Takeshita, Fumika Inagaki, Satohiro Masuda, Naoya Matsunaga, Satoru Koyanagi, Shigehiro Ohdo

      European Journal of Pharmacology800   48 - 56   4 2017

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1016/j.ejphar.2017.02.025

      Scopus

      PubMed

      researchmap

      Other Link: https://www.sciencedirect.com/science/article/pii/S0014299917300912?via%3Dihub

    • Disruption of mitochondrial fission in the liver protects mice from diet-induced obesity and metabolic deterioration Peer-reviewed International journal

      Lixiang Wang, Takaya Ishihara, Yuta Ibayashi, Keita Tatsushima, Daiki Setoyama, Yuki Hanada, Yukina Takeichi, Shohei Sakamoto, Sadaki Yokota, Katsuyoshi Mihara, Dongchon Kang, Naotada Ishihara, Ryoichi Takayanagi, Masatoshi Nomura

      Diabetologia58 ( 10 ) 2371 - 2380   24 10 2015

      More details

      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1007/s00125-015-3704-7

      Scopus

      PubMed

      researchmap

      Other Link: https://link.springer.com/article/10.1007/s00125-015-3704-7

    ▼display all

    Misc.

    • Preparation of mitochondria-enriched fraction from mammalian cells for in vitro import assay

      Yuki Hanada, Toshihiko Oka

      Jikken Igaku   13 11 2024

      More details

      Authorship:Lead author  

      researchmap

    • Molecular mechanism linking metabolism and innate immunity on mitochondria Peer-reviewed

      Yuki Hanada, Takaya Ishihara, Naotada Ishihara

      Journal of Japanese Biochemical Society94 ( 2 ) 230 - 235   25 4 2022

      More details

      Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

      DOI: 10.14952/SEIKAGAKU.2022.940230

      researchmap

    • Regulation of cellular functions by mitochondrial membrane dynamics

      Yuki Hanada, Takaya Ishihara, Naotada Ishihara

      Seitai no Kagaku69 ( 6 ) 581 - 585   12 2018

      More details

      Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

      researchmap

    Presentations

    • From Biogenesis to Function: Multi-step Control of Mff in Mitochondrial Dynamics and Antiviral Signaling Invited

      Yuki Hanada, Naotada Ishihara, Toshihiko Oka

      OsakaMito2025 Summer  29 8 2025 

      More details

      Event date: 27 8 2025 - 29 8 2025

      Language:English  

      researchmap

    • Mitochondrial outer membrane proteins regulated by energy metabolism for signaling at the mitochondrial surface Invited International conference

      Yuki Hanada

      Cold Spring Harbor Asia Conference, Mitochondria and Metabolism in Health and Disease  1 11 2023 

      More details

      Event date: 30 10 2023 - 3 11 2023

      Language:English   Presentation type:Oral presentation (invited, special)  

      Venue:Suzhou  

      I was selected as a chairperson of the session in this conference.

      researchmap

    • エネルギー代謝と免疫応答を繋ぐ ミトコンドリアの新機能

      花田有希

      立教大学 理学部 生命理学科 助教セミナー  13 10 2023 

      More details

    • Splicing-dependent functional regulation of mitochondrial fission factor Mff

      30 6 2022 

      More details

      Event date: 28 6 2022 - 30 6 2022

      Language:English   Presentation type:Poster presentation  

      researchmap

    • Two-sided control of antiviral response by mitochondrial immunometabolic factor Invited

      Yuki Hanada, Masatoshi Nomura, Yoshihiro Ogawa, Naotada Ishihara

      The 44th Annual Meeting of the Molecular Biology Society of Japan  2 12 2021 

      More details

      Event date: 1 12 2021 - 3 12 2021

      Language:English   Presentation type:Symposium, workshop panel (nominated)  

      researchmap

    • Molecular property of mitochondrial fission factor Mff for mitochondrial antiviral signaling

      3 11 2021 

      More details

      Event date: 3 11 2021 - 5 11 2021

      Language:Japanese   Presentation type:Poster presentation  

      researchmap

    • Regulatory mechanism of mitochondrial immune response against RNA virus infection International conference

      Yuki Hanada, Risa Maeda, Yoshihiro Ogawa, Masatoshi Nomura, Naotada Ishihara

      25 12 2020 

      More details

      Event date: 25 12 2020 - 26 12 2020

      Language:English   Presentation type:Oral presentation (general)  

      researchmap

    • Regulatory function of mitochondrial fission factor Mff in mitochondrial antiviral response

      26 6 2019 

      More details

      Event date: 25 6 2019 - 26 6 2019

      Language:Japanese   Presentation type:Oral presentation (general)  

      researchmap

    • ミトコンドリアを介した抗ウイルス応答におけるミトコンドリア分裂因子Mffの新たな調節機能

      花田 有希

      ミトコンドリアサイエンスワークショップ2019  14 5 2019 

      More details

      Event date: 14 5 2019 - 15 5 2019

      Language:Japanese   Presentation type:Oral presentation (general)  

      Venue:亀岡, 京都  

      researchmap

    • A novel regulatory function of mitochondrial fission factor Mff in mitochondrial antiviral activity

      Yuki Hanada, Takaya Ishihara, Masatoshi Nomura, Yoshihiro Ogawa, Naotada Ishihara

      26 9 2018 

      More details

      Event date: 24 9 2018 - 26 9 2018

      Language:Japanese   Presentation type:Oral presentation (general)  

      Young Scientist Award

      researchmap

    • Involvement of mitochondrial fission factor (Mff) in MAVS dissociation from mitochondria for a time-dependent induction of antiviral responses International conference

      Yuki Hanada, Lixiang Wang, Hidenori Otera, Takumi Koshiba, Syohei Sakamoto, Kenji Ashida, Naotada Ishihara, Katsuyoshi Mihara, Yoshihiro Ogawa, Masatoshi Nomura

      20 4 2018 

      More details

      Event date: 20 4 2018 - 22 4 2018

      Language:English   Presentation type:Poster presentation  

      Venue:Kyoto  

      researchmap

    ▼display all

    Teaching Experience

    • 2023 - Present 
      Experiments in Biology for Chemistry Students ( Rikkyo University )

      More details

    • 2023 - Present 
      Laboratory Experiments in Life Science 2B ( Rikkyo University )

      More details

    • 2023 - Present 
      Seminar in Life Science 2 ( Rikkyo University )

      More details

    • 2023 - Present 
      Basic Experiments in Life Science ( Rikkyo University )

      More details

    • 2023 - Present 
      Research Experiments ( Rikkyo University )

      More details

    Professional Memberships

    • 2024 - Present 
      The Japanese Society of Mitochondrial Research and Medicine (J-mit)

      More details

    • 2023 - Present 
      The Molecular Biology Society of Japan

      More details

    • 2019 - Present 
      Japan Society for Cell Biology

      More details

    • 2017 - Present 
      The Japanese Biochemical Society

      More details

    • 2015 - Present 
      The Japan Endocrine Society

      More details

    • 2014 - Present 
      The Pharmaceutical Society of Japan

      More details

    ▼display all

    Research Projects

    • Regulatory mechanism of mitochondrial function on mitochondrial outer membrane, the interface with the host

      the Japan Society for the Promotion of Science (JSPS)  Grants-in-Aid for Scientific Research (KAKENHI) 

      Yuki Hanada

      More details

      2023 - 2024

      Grant number:23K19355

      Authorship:Principal investigator 

      researchmap

    • Mitochondria-mediated metabolism-immune linkage for protection against viral infection

      Robert T. Huang Entrepreneurship Center of Kyushu University (QREC)  Academic Challenge 2017 grants-in-aid for scientific research 

      Yuki Hanada

      More details

      5 2017 - 3 2018

      Authorship:Principal investigator 

      Grant amount:\497000

      researchmap

    Academic Activities

    • Journal of Biochemistry reviewer

      Peer review

      2023

      More details

    Media Coverage

    • Mitochondria regulate strength of immune response Newspaper, magazine

      The Mainichi Newspapers  3 12 2020

      More details

    • Mitochondrial protein modulates defense against viral infection Newspaper, magazine

      The Nikkan Kogyo Newspapers  12 11 2020

      More details

    Other

    • Session 7 Chair in Cold Spring Harbor Asia Conference 2023

      11 2023
      -
      11 2023

      More details

    • JSPS PD Research Fellowship (refusal to accept fellowship)

      9 2022
      -
      9 2022

      More details