Updated on 2026/05/19

写真b

 
MASHIMA Keisuke
 
*Items subject to periodic update by Rikkyo University (The rest are reprinted from information registered on researchmap.)
Affiliation*
College of Science Department of Life Science
Graduate School of Science Doctoral Program in Life Science
Graduate School of Science Master's Program in Life Science
Title*
Senior Specially Appointed Professor
Degree
理学博士 / 理学博士 ( 大阪大学 )
Contact information
Mail Address
Research Interests
  • リン酸化・脱リン酸化

  • signal transduction

  • Protein tyrosine phosphatase

  • Campus Career*
    • 4 2024 - Present 
      College of Science   Department of Life Science   Senior Specially Appointed Professor
    • 4 2024 - Present 
      Graduate School of Science   Master's Program in Life Science   Senior Specially Appointed Professor
    • 4 2024 - Present 
      Graduate School of Science   Doctoral Program in Life Science   Senior Specially Appointed Professor
    • 4 2010 - 3 2024 
      College of Science   Department of Life Science   Professor
    • 4 2010 - 3 2024 
      Graduate School of Science   Master's Program in Life Science   Professor
    • 4 2010 - 3 2024 
      Graduate School of Science   Doctoral Program in Life Science   Professor
    • 4 2007 - 3 2010 
      College of Science   Department of Life Science   Associate Professor
    • 4 2007 - 3 2010 
      Graduate School of Science   Master's Program in Life Science   Associate Professor
    • 4 2007 - 3 2010 
      Graduate School of Science   Doctoral Program in Life Science   Associate Professor
    • 4 2002 - 3 2007 
      College of Science   Department of Life Science   Associate Professor (as old post name)
    • 4 2005 - 3 2007 
      Graduate School of Science   Master's Program in Life Science   Associate Professor (as old post name)
    • 4 2005 - 3 2007 
      Graduate School of Science   Doctoral Program in Life Science   Associate Professor (as old post name)
    • 4 2003 - 3 2004 
      Graduate School of Science   Doctoral Program in Life Science   Associate Professor (as old post name)
    • 4 1997 - 3 2002 
      College of Science   Department of Chemistry   Associate Professor (as old post name)
    • 4 1995 - 3 1997 
      College of Science   Department of Chemistry   Lecturer
    • 4 1989 - 3 1995 
      College of Science   Department of Chemistry   Full-time Research Assistant

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    Research Areas

    • Life Science / Immunology

    • Life Science / Functional biochemistry

    Research History

    • 4 2010 - Present 
      RIKKYO UNIVERSITY   Graduate School of Science Field of Study: Life Science   Professor

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    • 4 2010 - Present 
      RIKKYO UNIVERSITY   Graduate School of Science Field of Study: Life Science   Professor

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    • 4 2010 - Present 
      RIKKYO UNIVERSITY   College of Science Department of Life Science   Professor

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    • 4 2007 - 3 2010 
      RIKKYO UNIVERSITY   College of Science Department of Life Science   Associate Professor

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    • 4 2002 - 3 2007 
      RIKKYO UNIVERSITY   College of Science Department of Life Science   Associate Professor (as old post name)

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    • 4 1997 - 3 2002 
      RIKKYO UNIVERSITY   College of Science Department of Chemistry   Associate Professor (as old post name)

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    • 4 1995 - 3 1997 
      RIKKYO UNIVERSITY   College of Science Department of Chemistry   Lecturer

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    • 4 1989 - 3 1995 
      RIKKYO UNIVERSITY   College of Science Department of Chemistry   Full-time Research Assistant

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    • 6 1987 - 6 1988 
      岡崎国立共同研究機構・基礎生物学研究所   講師(PD相当)

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    Education

    • - 3 1987 
      Osaka University   Graduate School, Division of Natural Science

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      Country: Japan

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    • - 3 1984 
      Osaka University   Graduate School, Division of Natural Science

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      Country: Japan

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    • - 3 1981 
      University of Tsukuba   Second Cluster of College

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      Country: Japan

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    Papers

    • Muscarinic receptor stimulation induces TASK1 channel endocytosis through a PKC-Pyk2-Src pathway in PC12 cells. Peer-reviewed

      Matsuoka, H, Harada, K, Mashima, K, Inoue, M

      Cellular signaling65   109434   2020

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      Language:English   Publishing type:Research paper (scientific journal)  

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    • Mechanistic Insights into the Activation of Soluble Guanylate Cyclase by Carbon Monoxide: A Multi-Step Mechanism Proposed for the BAY 41-2272-Induced Formation of 5-Coordinate CO‒Heme. Peer-reviewed International journal

      Makino, R, Obata, Y, Tsubaki, M, Iizuka, T, Hamajima, Y, Kato-Yamada, Y, Mashima, K, Shiro, Y

      Biochemistry57 ( 10 ) 1620 - 1631   2018

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      Language:English   Publishing type:Research paper (scientific journal)  

      Soluble guanylate cyclase (sGC) is a heme-containing enzyme that catalyzes cGMP production upon sensing NO. While the CO adduct, sGC-CO, is much less active, the allosteric regulator BAY 41-2272 stimulates the cGMP productivity to the same extent as that of sGC-NO. The stimulatory effect has been thought to be likely associated with Fe-His bond cleavage leading to 5-coordinate CO-heme, but the detailed mechanism remains unresolved. In this study, we examined the mechanism under the condition including BAY 41-2272, 2'-deoxy-3'-GMP and foscarnet. The addition of these effectors caused the original 6-coordinate CO-heme to convert to an end product that was an equimolar mixture of a 5- and a new 6-coordinate CO-heme, as assessed by IR spectral measurements. The two types of CO-hemes in the end product were further confirmed by CO dissociation kinetics. Stopped-flow measurements under the condition indicated that the ferrous sGC bound CO as two reversible steps, where the primary step was assigned to the full conversion of the ferrous enzyme to the 6-coordinate CO-heme, and subsequently followed by the slower second step leading a partial conversion of the 6-coordinate CO-heme to the 5-coordinate CO-heme. The observed rates for both steps linearly depended on CO concentrations. The unexpected CO dependence of the rates in the second step supports a multistep mechanism, in which the 5-coordinate CO-heme is led by CO release from a putative bis-carbonyl intermediate that is likely provided by the binding of a second CO to the 6-coordinate CO-heme. This mechanism provides a new aspect on the activation of sGC by CO.

      DOI: 10.1021/acs.biochem.7b01240

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    • The Initial O2 Inserting Step of Tryptophan Dioxygenase Reaction Proposed by a Heme-Modification Study. Peer-reviewed

      Makino, R, Obayashi, E, Hori, H, Iizuka, T, Mashima, K, Shiro, Y, Ishimura, Y

      Biochemistry54   3604 - 3616   2015

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      Language:English  

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    • Protein tyrosine phosphatase-PEST (PTP-PEST) regulates mast cell-activating signals in PTP activity-dependent and -independent manners. Peer-reviewed

      Motohashi, S, Koizumi, K, Honda, R, Maruyama, A, Palmer, E.F. H, Mashima, K

      Cell Immunol.287   128 - 134   2014

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      Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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    • Protein Phosphatase 1alpha Associates to Protein Tyrosine Phosphatase-PEST inducing dephosphorylation of phospho-Serine 39. Peer-reviewed

      Nakamura, K, Palmer, E.E.H, Ozawa, T, Mashima, K

          2010

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      Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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    • PICOT, protein kinase C -theta interacting protein, is a novel regulator of FcRI-mediated mast cell activation. Peer-reviewed

      Kato, N, Motohashi, S, Okada, T, Ozawa, T, Mashima, K

      Cell Immunol.251 ( 1 ) 62 - 67   2008

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      Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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    • Intracellular localization and domain organization of human TRIM41 proteins. Peer-reviewed

      Tanaka, M, Fukuda, Y, Mashima, K, Hanai, R

      Mol. Biol. Rep32   87 - 93   2005

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    • Leupaxin binds to PEST domain tyrosine phosphatases PEP. Peer-reviewed

      Watanabe, N, Ishizuka, H, Amano, N, Mashima, K

      Mol. Cell. Biochem.269   13 - 17   2005

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      Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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    • Gene expression profile in the livers of rats orally administered ethinylestradiol for 28 days using a microarray technique. Peer-reviewed

      Kato, N, Shibutani, M, Takagi, H, Uneyama, C, Lee, K-Y, Takigami, S, Mashima, K, Hirose, M

      Toxicology30   179 - 192   2004

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    • Characterization of two isoforms of a human DnaJ homologue, HSJ2. Peer-reviewed

      Ryo Hanai, Keisuke Mashima

      Mol. Biol. Rep.30   149 - 153   2003

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    • Requirement of PEST domain tyrosine phosphatase PEP in B cell antigen receptor-induced growth arrest and apoptosis Peer-reviewed

      Kiminori Hasegawa, Hiroaki Yajima, Tatsuo Katagiri, Mami Ogimoto, Yutaka Arimura, Katsuyuki Mitomo, Keisuke Mashima, Kazuya Mizuno, Hidetaka Yakura

      European Journal of Immunology29 ( 3 ) 887 - 896   1999

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley-VCH Verlag  

      DOI: 10.1002/(SICI)1521-4141(199903)29:03<887::AID-IMMU887>3.0.CO;2-9

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    • Antigen receptor‐initiated growth inhibition is blocked in CD45‐loss variants of a mature B lymphoma, with limited effects on apoptosis Peer-reviewed

      Mami Ogimoto, Tatsuo Katagiri, Keisuke Mashima, Kiminori Hasegawa, Kazuya Mizuno, Hidetaka Yakura

      European Journal of Immunology25 ( 8 ) 2265 - 2271   1995

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      Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1002/eji.1830250823

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    • Adhesion-inducible DNA synthesis regulated by tyrosine phosphorylation/dephosphorylation

      Keisuke Mashima, Yuriko Furuya

      Biochemical and Biophysical Research Communications214 ( 3 ) 856 - 860   1995

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      Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

      DOI: 10.1006/bbrc.1995.2365

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    • 分子細胞生物学基礎実験法

      南江堂   424-439   4 1994

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      Language:Japanese   Publishing type:Research paper (other academic)  

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    • Alteration in protein-tyrosine phosphatase of rat epithelial cells by RSV-transformation: Application of phospho-tyrosyl glutamine synthetase to the study of protein-tyrosine phosphatase

      Keisuke Mashima, Youko Okajima, Junko Usui, Takeo Shimizu, Kinuko Kimura

      Journal of Biochemistry115 ( 2 ) 333 - 337   1994

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      Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/oxfordjournals.jbchem.a124338

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    • Negative regulation of apoptotic death in immature B cells by CD45 Peer-reviewed

      Mami Ogimoto, Tatsuo Katagiri, Keisuke Mashima, Kiminori Hasegawa, Kazuya Mizuno, Hidetaka Yakura

      Int. Immunology6   647 - 654   1 1 1994

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    • 遺伝子のクローニング

      眞島 恵介

      堀尾武一 分子細胞生物学基礎実験法 南江堂   424 - 439   1 1 1994

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      Language:Japanese   Publishing type:Research paper (other academic)  

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    • Changes in cell attachment by RSV-transforma-tion in rat epithelial cells

      Keisuke Mashima, Junko Mizuguchi

      Biochem. Biophys. Res. Commun.189   350-355   4 1992

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      Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (other academic)  

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    • Purification and properties of growth inhibitor from normal rabbit serum Peer-reviewed

      Takashi Kimura, Kaoru Miyazaki, Keisuke Mashima, Jinpei Yamashita, Takekazu Horio, Tomisaburo Kakuno

      Journal of Biochemistry110 ( 3 ) 423 - 328   1991

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/oxfordjournals.jbchem.a123597

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    • 増殖阻害因子の分子的多様性と生理活性 - 特に血清中の増殖阻害因子とラットの形質転換細胞RSV-BRLが分泌する増殖阻害因子について(共著)

          285-291   4 1989

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      Language:Japanese   Publishing type:Research paper (other academic)  

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    • Structure of expression of elongation factor 2 gene during development of Dictyostelium discoideum Peer-reviewed

      K. Toda, M. Tasaka, K. Mashima, K. Kohno, T. Uchida, I. Takeuchi

      Journal of Biological Chemistry264 ( 26 ) 15489 - 15493   1989

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    • 続生化学実験講座2 タンパク質の化学(上)(共著)

      角野富三郎, 眞島恵介, 山下仁平, 堀尾武一

      東京化学同人 日本生化学会編2   209-220   4 1988

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      Language:Japanese   Publishing type:Part of collection (book)  

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    • Multiple forms of growth inhibitors secreted from cultured rat liver cells: Purification and characterization

      Keisuke Mashima, Takashi Kimura, Weida Huang, Kazuo Yano, Yoshiyuki Ashida, Yoshiki Yamagata, Kaoru Miyazaki, Jinpei Yamashita, Takekazu Horio

      Journal of Biochemistry103 ( 6 ) 1020 - 1026   1988

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      Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/oxfordjournals.jbchem.a122373

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    • GROWTH-INHIBITORY PROTEIN PRESENT IN RABBIT SERUM, WHICH IS MORE EFFECTIVE ON TUMORIGENIC RAT-LIVER EPITHELIAL-CELLS THAN ON NONTUMORIGENIC ONES - ITS SPECIES, AND MODE OF EXISTENCE

      K MASHIMA, T KIMURA, K MIYAZAKI, J YAMASHITA, T HORIO

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS148 ( 3 ) 1215 - 1222   11 1987

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      Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

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    • TRANSFORMATION OF RAT-LIVER CELL-LINE BY ROUS-SARCOMA VIRUS CAUSES LOSS OF CELL-SURFACE FIBRONECTIN, ACCOMPANIED WITH SECRETION OF METALLOPROTEINASE THAT PREFERENTIALLY DIGESTS THE FIBRONECTIN Peer-reviewed

      K MIYAZAKI, Y ASHIDA, Y KIHIRA, K MASHIMA, J YAMASHITA, T HORIO

      JOURNAL OF BIOCHEMISTRY102 ( 3 ) 569 - 582   9 1987

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    • GROWTH-INHIBITORS IN SERUM, PLATELETS, AND NORMAL AND MALIGNANT-TISSUES Peer-reviewed

      K MIYAZAKI, K MASHIMA, T KIMURA, W HUANG, K YANO, Y ASHIDA, Y KIHIRA, J YAMASHITA, T HORIO

      ADVANCES IN ENZYME REGULATION26   225 - 237   1987

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    • Epithelial growth inhibitors in rat serum and human platelets(共著) Peer-reviewed

      Miyazaki, K., Mashima, K., Kimura, T., Huang, W., Masaki, H., Yamashita, J., Horio, T.

      Protides of the Biological Fluids34   553-556   4 1986

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    • Purification and properties of epithelial growth inhibitor (EGI) from human platelets: Its separation from type β transforming growth factor (TGF-β) Peer-reviewed

      Weida Huang, Takashi Kimura, Keisuke Mashima, Kaoru Miyazaki, Hiroaki Masaki, Jinpei Yamashtta, Takekazu Horio

      Journal of Biochemistry100 ( 3 ) 687 - 696   1986

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      Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press  

      DOI: 10.1093/oxfordjournals.jbchem.a121761

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    • Growth regulation of normal and RSV-transformed liver cells of rat by growth inhibitors presented in serum(共著)

      Proceeding of the International Symposium on Growth and Differentiation of Cell in Defined Environment, Springer-Verlag, Kohdansha, Belin, Tokyo,   443-448   4 1985

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    • Characterization of a growth-inhibiting protein present in rat serum that exerts a differential effect on in vitro growth of nonmalignant rat liver cells when compared with Rous sarcoma virus-transformed rat liver cells. Peer-reviewed

      Miyazaki K, Mashima K, Yamashita N, Yamashita J, Horio T

      In vitro cellular & developmental biology : journal of the Tissue Culture Association21   62 - 66   1 1985

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    • CHARACTERIZATION OF A GROWTH-INHIBITING PROTEIN PRESENT IN RAT SERUM THAT EXERTS A DIFFERENTIAL EFFECT ON INVITRO GROWTH OF NONMALIGNANT RAT-LIVER CELLS WHEN COMPARED WITH ROUS-SARCOMA VIRUS-TRANSFORMED RAT-LIVER CELLS Peer-reviewed

      K MIYAZAKI, K MASHIMA, N YAMASHITA, J YAMASHITA, T HORIO

      IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY21 ( 1 ) 62 - 66   1985

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    • 細胞増殖におけるプロテアーゼの役割(共著)

      宮崎 香, 山下信彦, 眞島恵介, 山下仁平, 堀尾武一

      日本応用酵素協会誌19   23-35   4 1984

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    • 癌細胞の増殖機構の研究(共著)

      宮崎 香, 眞島恵介, 山下信彦, 紀平安則, 山下仁平, 堀尾武一

      生物物理化学28   195-200   4 1984

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    Misc.

    • Requirement of pest domain tyrosine phosphatase PEP in BCR-induced growth arrest and apoptosis

      H Yajima, K Hasegawa, T Katagiri, M Ogimoto, Y Arimura, K Mitomo, K Mashima, K Mizuno, H Yakura

      FASEB JOURNAL13 ( 4 ) A330 - A330   3 1999

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      Language:English   Publishing type:Research paper, summary (international conference)  

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    Research Projects

    • ウニの初期胚発生におけるタンパク質チロシン残基のリン酸化・脱リン酸化の機能解析

      立教大学  立教大学研究奨励助成金 

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      4 1999 - 3 2000

      Grant type:Competitive

      個人研究

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    • 免疫担当細胞であるBリンパ球で発現している受容体型チロシンホスファターゼCD45と特異的に結合するリガンドの同定とその構造解析

      立教大学  立教大学研究奨励助成金 

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      4 1996 - 3 1997

      Grant type:Competitive

      個人研究

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    • 骨髄性白血病細胞のタンパク質チロシンホスファターゼ(PTP)の多様性と機能に関する研究

      金子・成田財団  受託研究(一般受託研究) 

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      4 1994 - 3 1996

      Grant type:Competitive

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    • 細胞の癌化における チロシンホスファターゼの役割に関する研究

      日本学術振興会  科学研究費助成事業 

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      4 1991 - 3 1992

      Grant type:Competitive

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    • 細胞の癌化及び分化におけるチロシン残基のリン酸化反応・脱リン酸化反応の役割に関する研究

      立教大学  立教大学研究奨励助成金 

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      4 1991 - 3 1992

      Grant type:Competitive

      個人研究

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    • A study of protein tyrosine phosphatase on signal transduction.

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      Grant type:Competitive

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    • チロシンホスファターゼによる細胞内情報伝達の制御機構

      フロンティア研究 

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      Grant type:Competitive

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    Social Contribution

    • 神経科学総合研究所

      4 1997 - Present

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      客員研究員

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