2022/11/25 更新

写真b

サカキバラ ケイコ
榊原 恵子
SAKAKIBARA Keiko
*大学が定期的に情報更新している項目(その他は、researchmapの登録情報を転載)
所属*
理学部 生命理学科
理学研究科 生命理学専攻 博士課程後期課程
理学研究科 生命理学専攻 博士課程前期課程
職名*
准教授
学位
博士(理学) ( 総合研究大学院大学 )
研究テーマ*
  • 陸上植物は約4億7千万年以上前にシャジクモ藻類のような祖先から分かれて進化してきたと考えられている。その過程で、新しい遺伝子の獲得や、もともと持っていた遺伝子が新しい機能を獲得したことが重要な役割を果たした。陸上植物やその近縁種の持っている遺伝子の配列やその遺伝子の機能を調べて比較することで、陸上植物の成立に関わった遺伝子進化の解明をめざす。

  • 研究キーワード
  • 藻類

  • コケ植物

  • 植物

  • 発生進化学

  • 学内職務経歴*
    • 2016年4月 - 現在 
      理学部   生命理学科   准教授
    • 2016年4月 - 現在 
      理学研究科   生命理学専攻 博士課程前期課程   准教授
    • 2016年4月 - 現在 
      理学研究科   生命理学専攻 博士課程後期課程   准教授
    プロフィール
    専門:植物の発生進化学。主にヒメツリガネゴケを用いた形態形成。
    略歴:2003年 総合研究大学院大学で学位取得後、広島大、オーストラリアMonash大にてポスドクを経て
    2009年 ERATO長谷部分化全能性プロジェクトにて技術参事、
    2011年 広島大学大学院理学研究科にて特任助教、
    2013年 東京大学大学院理学系研究科にて助教。
    2016年 立教大学理学部生命理学科にて准教授
     

    受賞

    • 2014年8月  
      日本進化学会研究奨励賞  陸上植物の生活史の発生進化学的研究
       
      榊原恵子

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    • 2013年11月  
      広島大学  広島大学学長表彰 
       
      榊原恵子

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    • 2013年9月  
      日本植物学会  奨励賞  ヒメツリガネゴケをモデルとした発生進化学的解析による陸上植物の異形世代交代を司る分子機構の解明
       
      榊原恵子

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    論文

    • Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis 査読有り 国際誌

      Shizuka Koshimizu, Naoki Minamino, Tomoaki Nishiyama, Emiko Yoro, Mayuko Sato, Mayumi Wakazaki, Kiminori Toyooka, Kazuo Ebine, Keiko Sakakibara, Takashi Ueda, Kentaro Yano

      New Phytologist   2022年8月3日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

      Land plant spermatozoids commonly possess characteristic structures such as the spline, which consists of a microtubule array, the multilayered structure (MLS) in which the uppermost layer is a continuum of the spline, and multiple flagella. However, the molecular mechanisms underpinning spermatogenesis remain to be elucidated. We successfully identified candidate genes involved in spermatogenesis, deeply divergent BLD10s, by computational analyses combining multiple methods and omics data. We then examined the functions of BLD10s in the liverwort Marchantia polymorpha and the moss Physcomitrium patens. MpBLD10 and PpBLD10 are required for normal basal body (BB) and flagella formation. Mpbld10 mutants exhibited defects in remodeling of the cytoplasm and nucleus during spermatozoid formation, and thus MpBLD10 should be involved in chromatin reorganization and elimination of the cytoplasm during spermiogenesis. We identified orthologs of MpBLD10 and PpBLD10 in diverse Streptophyta and found that MpBLD10 and PpBLD10 are orthologous to BLD10/CEP135 family proteins, which function in BB assembly. However, BLD10s evolved especially quickly in land plants and MpBLD10 might have acquired additional functions in spermatozoid formation through rapid molecular evolution.

      DOI: 10.1111/nph.18385

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      その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.18385

    • An ancestral function of strigolactones as symbiotic rhizosphere signals. 国際誌

      Kyoichi Kodama, Mélanie K Rich, Akiyoshi Yoda, Shota Shimazaki, Xiaonan Xie, Kohki Akiyama, Yohei Mizuno, Aino Komatsu, Yi Luo, Hidemasa Suzuki, Hiromu Kameoka, Cyril Libourel, Jean Keller, Keiko Sakakibara, Tomoaki Nishiyama, Tomomi Nakagawa, Kiyoshi Mashiguchi, Kenichi Uchida, Kaori Yoneyama, Yoshikazu Tanaka, Shinjiro Yamaguchi, Masaki Shimamura, Pierre-Marc Delaux, Takahito Nomura, Junko Kyozuka

      Nature communications13 ( 1 ) 3974 - 3974   2022年7月8日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      In flowering plants, strigolactones (SLs) have dual functions as hormones that regulate growth and development, and as rhizosphere signaling molecules that induce symbiosis with arbuscular mycorrhizal (AM) fungi. Here, we report the identification of bryosymbiol (BSB), an SL from the bryophyte Marchantia paleacea. BSB is also found in vascular plants, indicating its origin in the common ancestor of land plants. BSB synthesis is enhanced at AM symbiosis permissive conditions and BSB deficient mutants are impaired in AM symbiosis. In contrast, the absence of BSB synthesis has little effect on the growth and gene expression. We show that the introduction of the SL receptor of Arabidopsis renders M. paleacea cells BSB-responsive. These results suggest that BSB is not perceived by M. paleacea cells due to the lack of cognate SL receptors. We propose that SLs originated as AM symbiosis-inducing rhizosphere signaling molecules and were later recruited as plant hormone.

      DOI: 10.1038/s41467-022-31708-3

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    • The complete plastid genome sequence of the enigmatic moss, Takakia lepidozioides (Takakiopsida, Bryophyta): evolutionary perspectives on the largest collection of genes in mosses and the intensive RNA editing

      Atsushi Sadamitsu, Yuya Inoue, Keiko Sakakibara, Hiromi Tsubota, Tomio Yamaguchi, Hironori Deguchi, Tomoaki Nishiyama, Masaki Shimamura

      Plant Molecular Biology107 ( 4-5 ) 431 - 449   2021年11月

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

      DOI: 10.1007/s11103-021-01214-z

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      その他リンク: https://link.springer.com/article/10.1007/s11103-021-01214-z/fulltext.html

    • An Agrobacterium ‐mediated stable transformation technique for the hornwort model Anthoceros agrestis 査読有り

      Eftychios Frangedakis, Manuel Waller, Tomoaki Nishiyama, Hirokazu Tsukaya, Xia Xu, Yuling Yue, Michelle Tjahjadi, Andika Gunadi, Joyce Van Eck, Fay‐Wei Li, Péter Szövényi, Keiko Sakakibara

      New Phytologist   2021年7月19日

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      担当区分:最終著者, 責任著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

      DOI: 10.1111/nph.17524

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      その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/nph.17524

    • The Hornworts: Morphology, evolution and development 査読有り 国際誌

      Eftychios Frangedakis, Masaki Shimamura, Juan Carlos Villarreal, Fay‐Wei Li, Marta Tomaselli, Manuel Waller, Keiko Sakakibara, Karen S. Renzaglia, Péter Szövényi

      New Phytologist229 ( 2 ) 735 - 754   2020年9月15日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

      Extant land plants consist of two deeply divergent groups, tracheophytes and bryophytes, which shared a common ancestor some 500 million years ago. While information about vascular plants and the two of the three lineages of bryophytes, the mosses and liverworts, is steadily accumulating, the biology of hornworts remains poorly explored. Yet, as the sister group to liverworts and mosses, hornworts are critical in understanding the evolution of key land plant traits. Until recently, there was no hornwort model species amenable to systematic experimental investigation, which hampered detailed insight into the molecular biology and genetics of this unique group of land plants. The emerging hornwort model species, Anthoceros agrestis, is instrumental in our efforts to better understand not only hornwort biology but also fundamental questions of land plant evolution. To this end, here we provide an overview of hornwort biology and current research on the model plant A. agrestis to highlight its potential in answering key questions of land plant biology and evolution.

      DOI: 10.1111/nph.16874

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    • Anthoceros genomes illuminate the origin of land plants and the unique biology of hornworts 査読有り

      Fay-Wei Li, Tomoaki Nishiyama, Manuel Waller, Eftychios Frangedakis, Jean Keller, Zheng Li, Noe Fernandez-Pozo, Michael S. Barker, Tom Bennett, Miguel A. Blázquez, Shifeng Cheng, Andrew C. Cuming, Jan de Vries, Sophie de Vries, Pierre-Marc Delaux, Issa S. Diop, C. Jill Harrison, Duncan Hauser, Jorge Hernández-García, Alexander Kirbis, John C. Meeks, Isabel Monte, Sumanth K. Mutte, Anna Neubauer, Dietmar Quandt, Tanner Robison, Masaki Shimamura, Stefan A. Rensing, Juan Carlos Villarreal, Dolf Weijers, Susann Wicke, Gane K.-S. Wong, Keiko Sakakibara, Péter Szövényi

      Nature Plants6 ( 3 ) 259 - 272   2020年3月

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

      DOI: 10.1038/s41477-020-0618-2

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      その他リンク: http://www.nature.com/articles/s41477-020-0618-2

    • Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants 査読有り

      Asuka Higo, Tomokazu Kawashima, Michael Borg, Mingmin Zhao, Irene López-Vidriero, Hidetoshi Sakayama, Sean A. Montgomery, Hiroyuki Sekimoto, Dieter Hackenberg, Masaki Shimamura, Tomoaki Nishiyama, Keiko Sakakibara, Yuki Tomita, Taisuke Togawa, Kan Kunimoto, Akihisa Osakabe, Yutaka Suzuki, Katsuyuki T. Yamato, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, José M. Franco-Zorrilla, David Twell, Frédéric Berger, Takashi Araki

      Nature Communications9 ( 1 ) 5283   2018年12月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

      DOI: 10.1038/s41467-018-07728-3

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      その他リンク: http://www.nature.com/articles/s41467-018-07728-3

    • Class III HD-Zip activity coordinates leaf development in Physcomitrella patens 査読有り

      Hoichong Karen Yip, Sandra K. Floyd, Keiko Sakakibara, John L. Bowman

      DEVELOPMENTAL BIOLOGY419 ( 1 ) 184 - 197   2016年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      Land plant bodies develop from meristems, groups of pluripotent stem cells, which may persist throughout the life of a plant or, alternatively, have a transitory existence. Early diverging land plants exhibit indeterminate (persistent) growth in their haploid gametophytic generation, whereas later diverging lineages exhibit indeterminate growth in their diploid sporophytic generation, raising the question of whether genetic machinery directing meristematic functions was co-opted between generations. Class III HD-Zip (C3HDZ) genes are required for the establishment and maintenance of shoot apical meristems in flowering plants. We demonstrate that in the moss Physcomitrella patens, C3HDZ genes are expressed in transitory meristems in both the gametophytic and sporophytic generations, but not in the persistent shoot meristem of the gametyphyte. Loss-of-function of P. patens C3HDZ was engineered using ectopic expression of miR166, an endogenous regulator of C3HDZ gene activity. Loss of C3HDZ gene function impaired the function of gametophytic transitory meristematic activity but did not compromise the functioning of the persistent shoot apical meristem during the gametophyte generation. These results argue against a wholesale co-option of meristematic gene regulatory networks from the gametophyte to the sporophyte during land plant evolution, instead suggesting that persistent meristems with a single apical cell in P. patens and persistent complex meristems in flowering plants are regulated by different genetic programs. (C) 2016 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.ydbio.2016.01.012

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    • Technological Innovations Give Rise to a New Era of Plant Evolutionary Developmental Biology 査読有り

      K. Sakakibara

      GENOMES AND EVOLUTION OF CHAROPHYTES, BRYOPHYTES, LYCOPHYTES AND FERNS78   3 - 35   2016年

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      担当区分:責任著者   記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD  

      Land plants evolved from freshwater algaelike ancestors approximately 480 million years ago. Land plants developed many new morphological features during the evolution including the origin of a multicellular diploid sporophyte, a sporophytic apical meristem that produces complex body architecture, stomata and the production of lateral organs (leaves), vascular tissue, roots, seeds and flowers. Genetic changes drove the development of these morphological features and recent technological innovations in genomics and genetic modification technologies have allowed us to investigate these genetic changes. Here, I describe recent research on several transcription factors that contributed to the morphological evolution of land plants, including the homeodomain proteins KNOX, BELL and WOX, as well as transcription factors of the bHLH, NAC and FLO/LFY families. I also discuss the conservation of plant hormone signalling, which acts to coordinate the multicellular body plan.

      DOI: 10.1016/bs.abr.2016.01.001

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    • Evolution in the Cycles of Life 招待有り 査読有り

      John L. Bowman, Keiko Sakakibara, Chihiro Furumizu, Tom Dierschke

      ANNUAL REVIEW OF GENETICS, VOL 5050   133 - 154   2016年

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      記述言語:英語   掲載種別:論文集(書籍)内論文   出版者・発行元:ANNUAL REVIEWS  

      The life cycles of eukaryotes alternate between haploid and diploid phases, which are initiated by meiosis and gamete fusion, respectively. In both ascomycete and basidiomycete fungi and chlorophyte algae, the haploid-to-diploid transition is regulated by a pair of paralogous homeodomain protein encoding genes. That a common genetic program controls the haploid-to-diploid transition in phylogenetically disparate eukaryotic lineages suggests this may be the ancestral function for homeodomain proteins. Multicellularity has evolved independently in many eukaryotic lineages in either one or both phases of the life cycle. Organisms, such as land plants, exhibiting a life cycle whereby multicellular bodies develop in both the haploid and diploid phases are often referred to as possessing an alternation of generations. We review recent progress on understanding the genetic basis for the land plant alternation of generations and highlight the roles that homeodomain-encoding genes may have played in the evolution of complex multicellularity in this lineage.

      DOI: 10.1146/annurev-genet-120215-035227

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    • Antagonistic Roles for KNOX1 and KNOX2 Genes in Patterning the Land Plant Body Plan Following an Ancient Gene Duplication 査読有り

      Chihiro Furumizu, John Paul Alvarez, Keiko Sakakibara, John L. Bowman

      PLOS GENETICS11 ( 2 ) ee1004980   2015年2月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

      Neofunctionalization following gene duplication is thought to be one of the key drivers in generating evolutionary novelty. A gene duplication in a common ancestor of land plants produced two classes of KNOTTED-like TALE homeobox genes, class I (KNOX1) and class II (KNOX2). KNOX1 genes are linked to tissue proliferation and maintenance of meri-stematic potentials of flowering plant and moss sporophytes, and modulation of KNOX1 activity is implicated in contributing to leaf shape diversity of flowering plants. While KNOX2 function has been shown to repress the gametophytic (haploid) developmental program during moss sporophyte (diploid) development, little is known about KNOX2 function in flowering plants, hindering syntheses regarding the relationship between two classes of KNOX genes in the context of land plant evolution. Arabidopsis plants harboring loss-of-function KNOX2 alleles exhibit impaired differentiation of all aerial organs and have highly complex leaves, phenocopying gain-of-function KNOX1 alleles. Conversely, gain-of-function KNOX2 alleles in conjunction with a presumptive heterodimeric BELL TALE homeobox partner suppressed SAM activity in Arabidopsis and reduced leaf complexity in the Arabidopsis relative Cardamine hirsuta, reminiscent of loss-of-function KNOX1 alleles. Little evidence was found indicative of epistasis or mutual repression between KNOX1 and KNOX2 genes. KNOX proteins heterodimerize with BELL TALE homeobox proteins to form functional complexes, and contrary to earlier reports based on in vitro and heterologous expression, we find high selectivity between KNOX and BELL partners in vivo. Thus, KNOX2 genes confer opposing activities rather than redundant roles with KNOX1 genes, and together they act to direct the development of all above-ground organs of the Arabidopsis sporophyte. We infer that following the KNOX1/KNOX2 gene duplication in an ancestor of land plants, neofunctionalization led to evolution of antagonistic biochemical activity thereby facilitating the evolution of more complex sporophyte transcriptional networks, providing plasticity for the morphological evolution of land plant body plans.

      DOI: 10.1371/journal.pgen.1004980

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    • WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens 査読有り

      Keiko Sakakibara, Pascal Reisewitz, Tsuyoshi Aoyama, Thomas Friedrich, Sayuri Ando, Yoshikatsu Sato, Yosuke Tamada, Tomoaki Nishiyama, Yuji Hiwatashi, Tetsuya Kurata, Masaki Ishikawa, Hironori Deguchi, Stefan A. Rensing, Wolfgang Werr, Takashi Murata, Mitsuyasu Hasebe, Thomas Laux

      DEVELOPMENT141 ( 8 ) 1660 - 1670   2014年4月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

      Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In the moss Physcomitrella patens, cells at the wound margin of detached leaves become reprogrammed into stem cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like ( PpWOX13L) genes, homologs of stem cell regulators in flowering plants, are transiently upregulated and required for the initiation of cell growth during stem cell formation. Concordantly, Delta ppwox13l deletion mutants fail to upregulate genes encoding homologs of cell wall loosening factors during this process. During the moss life cycle, most of the Delta ppwox13l mutant zygotes fail to expand and initiate an apical stem cell to form the embryo. Our data show that PpWOX13L genes are required for the initiation of cell growth specifically during stem cell formation, in analogy to WOX stem cell functions in seed plants, but using a different cellular mechanism.

      DOI: 10.1242/dev.097444

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    • Evolution of the Class IV HD-Zip Gene Family in Streptophytes 査読有り

      Christopher S. Zalewski, Sandra K. Floyd, Chihiro Furumizu, Keiko Sakakibara, Dennis W. Stevenson, John L. Bowman

      MOLECULAR BIOLOGY AND EVOLUTION30 ( 10 ) 2347 - 2365   2013年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:OXFORD UNIV PRESS  

      Class IV homeodomain leucine zipper (C4HDZ) genes are plant-specific transcription factors that, based on phenotypes in Arabidopsis thaliana, play an important role in epidermal development. In this study, we sampled all major extant lineages and their closest algal relatives for C4HDZ homologs and phylogenetic analyses result in a gene tree that mirrors land plant evolution with evidence for gene duplications in many lineages, but minimal evidence for gene losses. Our analysis suggests an ancestral C4HDZ gene originated in an algal ancestor of land plants and a single ancestral gene was present in the last common ancestor of land plants. Independent gene duplications are evident within several lineages including mosses, lycophytes, euphyllophytes, seed plants, and, most notably, angiosperms. In recently evolved angiosperm paralogs, we find evidence of pseudogenization via mutations in both coding and regulatory sequences. The increasing complexity of the C4HDZ gene family through the diversification of land plants correlates to increasing complexity in epidermal characters.

      DOI: 10.1093/molbev/mst132

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    • KNOX2 Genes Regulate the Haploid-to-Diploid Morphological Transition in Land Plants 査読有り

      Keiko Sakakibara, Sayuri Ando, Hoichong Karen Yip, Yosuke Tamada, Yuji Hiwatashi, Takashi Murata, Hironori Deguchi, Mitsuyasu Hasebe, John L. Bowman

      SCIENCE339 ( 6123 ) 1067 - 1070   2013年3月

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      担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

      Unlike animals, land plants undergo an alternation of generations, producing multicellular bodies in both haploid (1n: gametophyte) and diploid (2n: sporophyte) generations. Plant body plans in each generation are regulated by distinct developmental programs initiated at either meiosis or fertilization, respectively. In mosses, the haploid gametophyte generation is dominant, whereas in vascular plants-including ferns, gymnosperms, and angiosperms-the diploid sporophyte generation is dominant. Deletion of the class 2 KNOTTED1-LIKE HOMEOBOX (KNOX2) transcription factors in the moss Physcomitrella patens results in the development of gametophyte bodies from diploid embryos without meiosis. Thus, KNOX2 acts to prevent the haploid-specific body plan from developing in the diploid plant body, indicating a critical role for the evolution of KNOX2 in establishing an alternation of generations in land plants.

      DOI: 10.1126/science.1230082

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    • Gametangia development in the moss Physcomitrella patens. 査読有り

      Kofuji R, Yoshimura T, Inoue H, Sakakibara K, Hiwatashi Y, Kurt T, Aoyama T, Ueda K, Hasebe N

      Annual Plant Reviews, Volume36 The moss <i>Physcomitrella patens</i>36   167 - 181   2009年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

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    • Class 1 KNOX genes are not involved in shoot development in the moss Physcomitrella patens but do function in sporophyte development 査読有り

      Keiko Sakakibara, Tomoaki Nishiyama, Hironori Deguchi, Mitsuyasu Hasebe

      EVOLUTION & DEVELOPMENT10 ( 5 ) 555 - 566   2008年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

      Although the number and form of metazoan organs are determined in the embryo, plants continuously form organs via pluripotent stem cells contained within the meristem. Flowering plants have an indeterminate meristem in their diploid generation, whereas the common ancestor of land plants is inferred to have formed an indeterminate meristem in its haploid generation, as observed in the extant basal land plants, bryophytes, including mosses. It is hypothesized that the underlying gene networks for the diploid meristem were initially present in the haploid generation of the basal land plants and were eventually co-opted for expression in the diploid generation. In flowering plants, the class 1 KNOTTED1-LIKE HOMEOBOX (KNOX) transcription factors are essential for the function of the indeterminate apical meristem. Here, we show that the class 1 KNOX orthologs function in the diploid organ, with determinate growth in the moss Physcomitrella patens, but do not function in the haploid indeterminate meristem. We propose that the genetic networks governing the indeterminate meristem in land plants are variable, and the networks governing the diploid indeterminate meristem with the class 1 KNOX genes likely evolved de novo in the flowering plant lineage.

      DOI: 10.1111/j.1525-142X.2008.00271.x

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    • The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants 査読有り

      Stefan A. Rensing, Daniel Lang, Andreas D. Zimmer, Astrid Terry, Asaf Salamov, Harris Shapiro, Tomoaki Nishiyama, Pierre-Francois Perroud, Erika A. Lindquist, Yasuko Kamisugi, Takako Tanahashi, Keiko Sakakibara, Tomomichi Fujita, Kazuko Oishi, Tadasu Shin-I, Yoko Kuroki, Atsushi Toyoda, Yutaka Suzuki, Shin-ichi Hashimoto, Kazuo Yamaguchi, Sumio Sugano, Yuji Kohara, Asao Fujiyama, Aldwin Anterola, Setsuyuki Aoki, Neil Ashton, W. Brad Barbazuk, Elizabeth Barker, Jeffrey L. Bennetzen, Robert Blankenship, Sung Hyun Cho, Susan K. Dutcher, Mark Estelle, Jeffrey A. Fawcett, Heidrun Gundlach, Kousuke Hanada, Alexander Heyl, Karen A. Hicks, Jon Hughes, Martin Lohr, Klaus Mayer, Alexander Melkozernov, Takashi Murata, David R. Nelson, Birgit Pils, Michael Prigge, Bernd Reiss, Tanya Renner, Stephane Rombauts, Paul J. Rushton, Anton Sanderfoot, Gabriele Schween, Shin-Han Shiu, Kurt Stueber, Frederica L. Theodoulou, Hank Tu, Yves Van de Peer, Paul J. Verrier, Elizabeth Waters, Andrew Wood, Lixing Yang, David Cove, Andrew C. Cuming, Mitsuyasu Hasebe, Susan Lucas, Brent D. Mishler, Ralf Reski, Igor V. Grigoriev, Ralph S. Quatrano, Jeffrey L. Boore

      SCIENCE319 ( 5859 ) 64 - 69   2008年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

      We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments ( e. g., flagellar arms); acquisition of genes for tolerating terrestrial stresses ( e. g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant&apos;s unique facility for reverse genetics.

      DOI: 10.1126/science.1150646

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    • Green genes - Comparative genomics of the green branch of life 査読有り

      John L. Bowman, Sandra K. Floyd, Keiko Sakakibara

      CELL129 ( 2 ) 229 - 234   2007年4月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

      As more plant genome sequences become available, researchers are increasingly using comparative genomics to address some of the major questions in plant biology. Such questions include the evolution of photosynthesis and multicellularity, the developmental genetic changes responsible for alterations in body plan, and the origin of important plant innovations such as roots, leaves, and vascular tissue.

      DOI: 10.1016/j.cell.2007.04.004

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    • KNOX homeobox genes potentially have similar function in both diploid unicellular and multicellular meristems, but not in haploid meristems 査読有り

      R Sano, CM Juarez, B Hass, K Sakakibara, M Ito, JA Banks, M Hasebe

      EVOLUTION & DEVELOPMENT7 ( 1 ) 69 - 78   2005年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BLACKWELL PUBLISHING INC  

      Members of the class 1 knotted-like homeobox (KNOX) gene family are important regulators of shoot apical meristem development in angiosperms. To determine whether they function similarly in seedless plants, three KNOX genes (two class 1 genes and one class 2 gene) from the fern Ceratopteris richardii were characterized. Expression of both class 1 genes was detected in the shoot apical cell, leaf primordia, marginal part of the leaves, and vascular bundles by in situ hybridization, a pattern that closely resembles that of class 1 KNOX genes in angiosperms with compound leaves. The fern class 2 gene was expressed in all sporophyte tissues examined, which is characteristic of class 2 gene expression in angiosperms. All three CRKNOX genes were not detected in gametophyte tissues by RNA gel blot analysis. Arabidopsis plants overexpressing the fern class 1 genes resembled plants that overexpress seed plant class 1 KNOX genes in leaf morphology. Ectopic expression of the class 2 gene in Arabidopsis did not result in any unusual phenotypes. Taken together with phylogenetic analysis, our results suggest that (a) the class 1 and 2 KNOX genes diverged prior to the divergence of fern and seed plant lineages, (b) the class 1 KNOX genes function similarly in seed plant and fern sporophyte meristem development despite their differences in structure, (c) KNOX gene expression is not required for the development of the fern gametophyte, and (d) the sporophyte and gametophyte meristems of ferns are not regulated by the same developmental mechanisms at the molecular level.

      DOI: 10.1111/j.1525-142X.2005.05008.x

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    • Involvement of auxin and a homeodomain-leucine zipper I gene in rhizoid development of the moss Physcomitrella patens 査読有り

      K Sakakibara, T Nishiyama, N Sumikawa, R Kofuji, T Murata, M Hasebe

      DEVELOPMENT130 ( 20 ) 4835 - 4846   2003年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:COMPANY OF BIOLOGISTS LTD  

      Differentiation of epidermal cells is important for plants because they are in direct contact with the environment. Rhizoids are multicellular filaments that develop from the epidermis in a wide range of plants, including pteridophytes, bryophytes, and green algae; they have similar functions to root hairs in vascular plants in that they support the plant body and are involved in water and nutrient absorption. In this study, we examined mechanisms underlying rhizoid development in the moss, Physcomitrella patens, which is the only land plant in which high-frequency gene targeting is possible. We found that rhizoid development can be split into two processes: determination and differentiation. Two types of rhizoids with distinct developmental patterns (basal and mid-stem rhizoids) were recognized. The development of basal rhizoids from epidermal cells was induced by exogenous auxin, while that of mid-stem rhizoids required an unknown factor in addition to exogenous auxin. Once an epidermal cell had acquired a rhizoid initial cell fate, expression of the homeodomain-leucine zipper I gene Pphb7 was induced. Analysis of Pphb7 disruptant lines showed that Pphb7 affects the induction of pigmentation and the increase in the number and size of chloroplasts, but not the position or number of rhizoids. This is the first report on the involvement of a homeodomain-leucine zipper I gene in epidermal cell differentiation.

      DOI: 10.1242/dev.00644

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    • Characterization of a FLORICAULA/LEAFY homologue of Gnetum parvifolium and its implications for the evolution of reproductive organs in seed plants 査読有り

      S Shindo, K Sakakibara, R Sano, K Ueda, M Hasebe

      INTERNATIONAL JOURNAL OF PLANT SCIENCES162 ( 6 ) 1199 - 1209   2001年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:UNIV CHICAGO PRESS  

      The morphological variation among reproductive organs of extant seed plants makes assessment of organ homology difficult. Comparisons of expression patterns of homeotic genes that control organ development will yield new information about the homology of organs to assess inferences deduced from previous morphological studies. In angiosperms, the FLORICAULA/LEAFY (FLO/LFY) genes convert a vegetative shoot meristem to a floral meristem by inducing floral homeotic genes, most of which belong to the MADS-box gene family. To provide insights into the evolution of reproductive organs in seed plants, a FLO/LFY homologue (GpLFY) was cloned from Gnetum parvifolium. GpLFY mRNA was expressed in both the vegetative shoot apex and the female strobilus. The GpLFY mRNA signal was detected in early developmental stages of the collar and the ovule primordium, including the nucellus and three envelopes. A comparison of FLO/LFY gene expression in Gnetum and the conifer Pinus radiata indicates that the Gnetum collar and ovule are homologous with the conifer bract and ovule-ovuliferous scale complex, respectively. Overexpression of GpLFY in transgenic Arabidopsis promoted a conversion of a shoot meristem to a floral primordium. The Arabidopsis LFY null mutant, lfy-26, with a malformed flower, was complemented by overexpression of GpLFY. These results indicate that the inductive pathway from the FLO/LFY gene to the MADS-box genes already existed in the common ancestor of angiosperms and gymnosperms.

      DOI: 10.1086/323417

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    • Isolation of homeodomain-leucine zipper genes from the moss Physcomitrella patens and the evolution of homeodomain-leucine zipper genes in land plants 査読有り

      K. Sakakibara, T. Nishiyama, M. Kato, M. Hasebe

      Molecular Biology and Evolution18 ( 4 ) 491 - 502   2001年

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      担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Society for Molecular Biology and Evolution  

      Homeobox genes encode transcription factors involved in many aspects of developmental processes. The homeodomain-leucine zipper (HD-Zip) genes, which are characterized by the presence of both a homeodomain and a leucine zipper motif, form a clade within the homeobox superfamily and were previously reported only from vascular plants. Here we report the isolation of 10 HD-Zip genes (named Pphb1-Pphb10) from the moss Physcomitrella patens. Based on a phylogenetic analysis of the 10 Pphb genes and previously reported vascular plant HD-Zip genes, all of the Pphb genes except Pphb3 belong to three of the four HD-Zip subfamilies (HD-Zip I, II, and III), indicating that these subfamilies originated before the divergence of the vascular plant and moss lineages. Pphb3 is sister to the HD-Zip II subfamily and has some distinctive characteristics, including the difference of the a1 and d1 sites of its leucine zipper motif, which are well conserved in each HD-Zip subfamily. Comparison of the genetic divergence of representative HD-Zip I and II genes showed that the evolutionary rate of HD-Zip I genes was faster than that of HD-Zip II genes.

      DOI: 10.1093/oxfordjournals.molbev.a003828

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    • Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis 査読有り

      Tomoaki Nishiyama, Yuji Hiwatashi, Keiko Sakakibara, Masahiro Kato, Mitsuyasu Hasebe

      DNA Research7 ( 1 ) 9 - 17   2000年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Universal Academy Press Inc.  

      The moss, Physcomitrella patens has been used as a useful material in many fields, because of its simple body plan, ease of gene targeting, and other reasons. Although many mutants have been reported, no method to isolate the corresponding genes was reported. We developed a gene tagging and gene-trap system in P. patens by using the shuttle mutagenesis technique, which has been used in the budding yeast. In 5264 tagged lines, 203 mutants with altered developmental or morphological phenotypes were obtained. In 129 of 4757 gene-trap lines, β-glucuronidase (GUS) activity was detected in some tissue. Although multiple copies of a tag were detected in many tagged lines by Southern analyses, most copies are likely integrated at the same locus according to PCR analyses.

      DOI: 10.1093/dnares/7.1.9

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    MISC

    • コケ植物が語る過去・現在・未来 1 ツノゴケ-ゲノム解読と形質転換技術が拓く植物進化研究の新機軸

      西山智明, 榊原恵子, 嶋村正樹

      生物の科学 遺伝76 ( 3 )   2022年

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    • ツノゴケゲノムと陸上植物の発生進化 査読有り

      西山智明, 嶋村正樹, 榊原恵子

      植物科学の最前線12   186 - 195   2021年7月

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      記述言語:日本語   掲載種別:記事・総説・解説・論説等(その他)  

      DOI: 10.24480/bsj-review.12d2.00215

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    • 陸上植物起源研究の最後のフロンティア,ツノゴケの生物学 招待有り 査読有り

      嶋村正樹, 西山智明, 榊原恵子

      植物科学最前線12   183 - 185   2021年7月

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      担当区分:最終著者   記述言語:日本語  

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    • ゲノム解析から見たツノゴケの二酸化炭素濃縮機構とシアノバクテリア,菌類との共生 植物の陸上進出を可能にした生存戦略

      西山智明, 榊原恵子, 嶋村正樹

      化学と生物59 ( 10 )   2021年

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    • 古い酒を新しい革袋に~preexisting gene regulatory network の転用による 陸上植物のボディプラン革新 招待有り 査読有り

      石崎 公庸, 榊原 恵子

      植物科学の最前線7   47 - 54   2016年4月

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      記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:日本植物学会  

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    • KNOX2遺伝子は陸上植物の世代交代を制御する(一般講演,<特集>日本蘚苔類学会第42回岡山大会)

      榊原 恵子, 安藤 沙友里, Yip Hoichong Karen, 玉田 洋介, 日渡 祐二, 村田 隆, 出口 博則, 長谷部 光泰, Bowman John L.

      蘚苔類研究10 ( 12 ) 426 - 427   2013年11月

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

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    • 蘚苔類の世代交代とその制御因子

      榊原 恵子

      蘚苔類研究10 ( 12 ) 393 - 397   2013年11月

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

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    • 日本植物学会第76回大会参加およびシンポジウム開催報告(掲示板)

      嶋村 正樹, 榊原 恵子

      蘚苔類研究10 ( 9 ) 288 - 290   2012年12月

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

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    • ヤマトツノゴケモドキの胞子体の組織発生学的研究(ポスター発表,<特集>日本蘚苔類学会第41回北海道大会)

      奥田 有貴, 榊原 恵子, 嶋村 正樹, 山口 富美夫, 出口 博則

      蘚苔類研究10 ( 9 ) 301 - 301   2012年

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

      DOI: 10.24474/bryologicalresearch.10.9_301_1

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    • 西表島における生葉上苔類と被着生植物の関係(ポスター発表,<特集>日本蘚苔類学会第41回北海道大会)

      友岡 秀文, 榊原 恵子, 嶋村 正樹, 山口 富美夫, 出口 博則

      蘚苔類研究10 ( 9 ) 303 - 303   2012年

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

      DOI: 10.24474/bryologicalresearch.10.9_303_2

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    • 蛍光顕微鏡を用いたツノゴケ類胞子体の形態学的研究(ポスター発表,<特集>日本蘚苔類学会第40回奈良大会)

      奥田 有貴, 山口 富美夫, 嶋村 正樹, 榊原 恵子, 出口 博則

      蘚苔類研究10 ( 6 ) 175 - 175   2011年

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      記述言語:日本語   出版者・発行元:日本蘚苔類学会  

      DOI: 10.24474/bryologicalresearch.10.6_175_1

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    • Functional analyses of class 2 KNOX genes in Physcomitrella patens

      K Sakakibara, H Deguchi, M Hasebe

      PLANT AND CELL PHYSIOLOGY47   S180 - S180   2006年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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    • Functional analysis of class 1 KNOX genes in Physcomitrella patens

      T Nishiyama, K Sakakibara, M Hasebe

      PLANT AND CELL PHYSIOLOGY46   S198 - S198   2005年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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    • Functional Analysis of KNOX class 1 genes in Physcomitrella patens

      T Nishiyama, K Sakakibara, M Hasebe

      PLANT AND CELL PHYSIOLOGY45   S162 - S162   2004年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:OXFORD UNIV PRESS  

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    • The role of Pphb7,a Physcomitrella HD-Zip gene, in rhizoid formation :

      SAKAKIBARA Keiko, HASEBE Mitsuyasu

      Plant and cell physiology42   s50   2001年

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      記述言語:英語   出版者・発行元:Japanese Society of Plant Physiologists  

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      その他リンク: https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=184659

    • コケ植物, ニセツリガネゴケにおけるMADS遺伝子の単離と遺伝子ターゲティングによる機能解析

      小藤 累美子, 榊原 恵子, 西山 智明, 長谷部 光泰

      日本分子生物学会年会プログラム・講演要旨集21   635 - 635   1998年12月1日

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      記述言語:日本語  

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    • Physcomitrella patensにおけるMADS遺伝子の単離と解析

      小藤 累美子, 榊原 恵子, 西山 智明, 長谷部 光泰

      日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan62   95 - 95   1998年9月1日

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      記述言語:日本語  

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    • Physcomiterella patensにおけるホメオボックス遺伝子の単離と解析

      榊原 恵子, 西山 智明, 小藤 累美子, 加藤 雅啓, 長谷部 光泰

      日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan62   95 - 95   1998年9月1日

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      記述言語:日本語  

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    • Physcomitrella patensのタグつき変異体ライブラリーの作成

      西山 智明, 榊原 恵子, 小藤 累美子, 加藤 雅啓, 長谷部 光泰

      日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan62   156 - 156   1998年9月1日

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      記述言語:日本語  

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