2021/10/27 更新

写真b

マエカワ シュウゴ
前川 修吾
MAEKAWA Shugo
*大学が定期的に情報更新している項目(その他は、researchmapの登録情報を転載)
所属*
理学部 生命理学科
職名*
助教
学内職務経歴*
  • 2018年4月 - 現在 
    理学部   生命理学科   助教
 

研究分野

  • ライフサイエンス / 応用分子細胞生物学

  • ライフサイエンス / 分子生物学  / 植物分子生物学

経歴

  • 2018年4月 - 現在 
    立教大学   理学部   助教

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  • 2015年4月 - 2018年3月 
    東京大学   生物生産工学研究センター   学振特別研究員(PD)

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  • 2014年4月 - 2015年3月 
    東京大学   生物生産工学研究センター   特任研究員

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  • 2013年4月 - 2014年3月 
    北海道大学大学院   理学研究員生物科学部門   学術研究員

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  • 2010年4月 - 2013年3月 
    北海道大学大学院   生命科学院   学振特別研究員(DC1)

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学歴

  • 2010年4月 - 2013年3月 
    北海道大学大学院   生命科学院   生命科学専攻 博士課程

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  • 2008年4月 - 2010年3月 
    北海道大学大学院   生命科学院   生命科学専攻 修士課程

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  • 2004年4月 - 2008年3月 
    北海道大学   理学部   生物学科

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受賞

  • 2020年9月  
    日本植物バイオテクノロジー学会  奨励賞  「植物のストレス応答機構に関する分子生物学的研究」
     
    前川修吾

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  • 2015年6月  
    東京大学生命科学ネットワーク  優秀ポスター賞 
     
    前川修吾

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  • 2009年  
    北海道大学先端生命研究院  研究者養成支援金 
     
    前川修吾

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論文

  • Ribosome biogenesis factor OLI2 and its interactor BRX1-2 are associated with morphogenesis and lifespan extension in <i>Arabidopsis thaliana</i> 査読有り

    Shugo Maekawa, Shuichi Yanagisawa

    Plant Biotechnology38 ( 1 ) 117 - 125   2021年3月25日

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Japanese Society for Plant Cell and Molecular Biology  

    DOI: 10.5511/plantbiotechnology.20.1224a

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  • The bRPS6-Family Protein RFC3 Prevents Interference by the Splicing Factor CFM3b during Plastid rRNA Biogenesis in Arabidopsis thaliana 査読有り 国際誌

    Yumi Nagashima, Katsutomo Ohshiro, Akiyasu Iwase, Miyuki T. Nakata, Shugo Maekawa, Gorou Horiguchi

    Plants9 ( 3 ) 328 - 328   2020年3月4日

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MDPI AG  

    Plastid ribosome biogenesis is important for plant growth and development. REGULATOR OF FATTY ACID COMPOSITION3 (RFC3) is a member of the bacterial ribosomal protein S6 family and is important for lateral root development. rfc3-2 dramatically reduces the plastid rRNA level and produces lateral roots that lack stem cells. In this study, we isolated a suppressor of rfc three2 (sprt2) mutant that enabled recovery of most rfc3 mutant phenotypes, including abnormal primary and lateral root development and reduced plastid rRNA level. Northern blotting showed that immature and mature plastid rRNA levels were reduced, with the exception of an early 23S rRNA intermediate, in rfc3-2 mutants. These changes were recovered in rfc3-2 sprt2-1 mutants, but a second defect in the processing of 16S rRNA appeared in this line. The results suggest that rfc3 mutants may be defective in at least two steps of plastid rRNA processing, one of which is specifically affected by the sprt2-1 mutation. sprt2-1 mutants had a mutation in CRM FAMILY MEMBER 3b (CFM3b), which encodes a plastid-localized splicing factor. A bimolecular fluorescence complementation (BiFC) assay suggested that RFC3 and SPRT2/CFM3b interact with each other in plastids. These results suggest that RFC3 suppresses the nonspecific action of SPRT2/CFM3b and improves the accuracy of plastid rRNA processing.

    DOI: 10.3390/plants9030328

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  • Overexpression of a Brix Domain-containing Ribosome Biogenesis Factor ARPF2 and Its Interactor ARRS1 Causes Morphological Changes and Lifespan Extension in Arabidopsis thaliana 査読有り

    Maekawa S, Ueda Y, Yanagisawa S

    Frontiers in Plant Science   2018年8月

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    担当区分:筆頭著者  

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  • Nucleolar stress and sugar response 査読有り

    Maekawa S, Yanagisawa S

    Plant Signaling and Behavior   e1442975   2018年2月

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    担当区分:筆頭著者  

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  • Reduced expression of APUM24, encoding a novel rRNA processing factor, induces sugar-dependent nucleolar stress and altered sugar responses in arabidopsis thaliana 査読有り

    Shugo Maekawa, Tetsuya Ishida, Shuichi Yanagisawa

    Plant Cell30 ( 1 ) 209 - 227   2018年1月1日

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:American Society of Plant Biologists  

    Ribosome biogenesis is one of the most energy-consuming events in the cell and must therefore be coordinated with changes in cellular energy status. Here, we show that the sugar-inducible gene ARABIDOPSIS PUMILIO PROTEIN24 (APUM24) encodes a Pumilio homology domain-containing protein involved in pre-rRNA processing in Arabidopsis thaliana. Null mutation of APUM24 resulted in aborted embryos due to abnormal gametogenesis and embryogenesis, whereas reduced expression of APUM24 caused several phenotypes characteristic of ribosome biogenesis or function-related mutants. APUM24 interacted with other pre-rRNA processing factors and a putative endonuclease for the removal of the internal transcribed spacer 2 (ITS2) of prerRNA in the nucleolus. The APUM24-containing complex also interacted with ITS2, and reduced APUM24 expression caused the overaccumulation of processing intermediates containing ITS2. Thus, APUM24 likely functions as an ITS2 removal-associated factor. Most importantly, the apum24 knockdown mutant was hypersensitive to highly concentrated sugar, and the mutant showed sugar-dependent overaccumulation of processing intermediates and nucleolar stress (changes in nucleolar size). Furthermore, reduced APUM24 expression diminished sugar-induced promotion of leaf and root growth. Hence, a breakdown in the coordinated expression of ribosome biogenesis-related genes with energy status may induce nucleolar stress and disturb proper sugar responses in Arabidopsis.

    DOI: 10.1105/tpc.17.00778

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  • Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks 査読有り

    Kun-hsiang Liu, Yajie Niu, Mineko Konishi, Yue Wu, Hao Du, Hoo Sun Chung, Lei Li, Marie Boudsocq, Matthew McCormack, Shugo Maekawa, Tetsuya Ishida, Chao Zhang, Kevan Shokat, Shuichi Yanagisawa, Jen Sheen

    NATURE545 ( 7654 ) 311 - +   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca2+-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca2+ signalling network integrates transcriptome and cellular metabolism with shoot-root coordination and developmental plasticity in shaping organ biomass and architecture.

    DOI: 10.1038/nature22077

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  • Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis 査読有り

    Takeo Sato, Shugo Maekawa, Mineko Konishi, Nozomi Yoshioka, Yuki Sasaki, Haruna Maeda, Tetsuya Ishida, Yuki Kato, Junji Yamaguchi, Shuichi Yanagisawa

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS483 ( 1 ) 380 - 386   2017年1月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Nitrate modulates growth and development, functioning as a nutrient signal in plants. Although many changes in physiological processes in response to nitrate have been well characterized as nitrate responses, the molecular mechanisms underlying the nitrate response are not yet fully understood. Here, we show that NLP transcription factors, which are key regulators of the nitrate response, directly activate the nitrate-inducible expression of BT1 and BT2 encoding putative scaffold proteins with a plant-specific domain structure in Arabidopsis. Interestingly, the 35S promoter-driven expression of BT2 partially rescued growth inhibition caused by reductions in NLP activity in Arabidopsis. Furthermore, simultaneous disruption of BT1 and BT2 affected nitrate-dependent lateral root development. These results suggest that direct activation of BT1 and BT2 by NLP transcriptional activators is a key component of the molecular mechanism underlying the nitrate response in Arabidopsis. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2016.12.135

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  • The Pre-rRNA Processing Complex in Arabidopsis Includes Two WD40-Domain-Containing Proteins Encoded by Glucose-Inducible Genes and Plant-Specific Proteins 査読有り

    Tetsuya Ishida*, Shugo Maekawa*, Shuichi Yanagisawa

    MOLECULAR PLANT9 ( 2 ) 312 - 315   2016年2月

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    担当区分:筆頭著者   記述言語:英語   出版者・発行元:CELL PRESS  

    DOI: 10.1016/j.molp.2015.11.003

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  • The Arabidopsis ubiquitin ligase ATL31 is transcriptionally controlled by WRKY33 transcription factor in response to pathogen attack 査読有り

    Thais Huarancca Reyes, Shugo Maekawa, Takeo Sato, Junji Yamaguchi

    PLANT BIOTECHNOLOGY32 ( 1 ) 11 - +   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    ATL31, an Arabidopsis RING-type ubiquitin ligase, plays a critical role in plant carbon/nitrogen (C/N)-nutrient responses during post-germinative growth and in defense responses to pathogen attack. ATL31 expression under these stress conditions suggested the presence of transcriptional regulators mediated by these stress signals. We recently reported that the expression pattern of WRKY33, a transcription factor involved in plant defense responses, is highly correlated with that of ATL31. In this study, we investigated the detailed relationship between the ATL31 gene and WRKY33. Using transient reporter analysis, we found that WRKY33 could significantly activate ATL31 transcription in plant cells. Transcript analysis of stable transgenic Arabidopsis plants overexpressing WRKY33 confirmed that the expression of ATL31 in response to the PAMPs flg22 and chitin was enhanced compared with wild-type plants, while expression was repressed in wrky33 mutants. Further detailed transient reporter analysis revealed that transactivation by WRKY33 is required and mediated through a specific W-box cis-acting element in the promoter region of the ATL31 gene. In contrast, WRKY33 did not regulate ATL31 expression during the C/N response. Taken together, these results demonstrate that WRKY33 acts as a transcription factor of ATL31 and positively regulates its expression during activation of plant defense responses.

    DOI: 10.5511/plantbiotechnology.14.1201b

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  • Pale-Green Phenotype of atl31 atl6 Double Mutant Leaves Is Caused by Disruption of 5-Aminolevulinic Acid Biosynthesis in Arabidopsis thaliana 査読有り

    Shugo Maekawa, Atsushi Takabayashi, Thais Huarancca Reyes, Hiroko Yamamoto, Ayumi Tanaka, Takeo Sato, Junji Yamaguchi

    PLOS ONE10 ( 2 ) e0117662   2015年2月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Arabidopsis ubiquitin ligases ATL31 and homologue ATL6 control the carbon/nitrogen nutrient and pathogen responses. A mutant with the loss-of-function of both atl31 and atl6 developed light intensity-dependent pale-green true leaves, whereas the single knockoutmutants did not. Plastid ultrastructure and Blue Native-PAGE analyses revealed that pale-green leaves contain abnormal plastid structure with highly reduced levels of thylakoid proteins. In contrast, the pale-green leaves of the atl31/atl6 mutant showed normal Fv/Fm. In the pale-green leaves of the atl31/atl6, the expression of HEMA1, which encodes the key enzyme for 5-aminolevulinic acid synthesis, the rate-limiting step in chlorophyll biosynthesis, was markedly down-regulated. The expression of key transcription factor GLK1, which directly promotes HEMA1 transcription, was also significantly decreased in atl31/atl6 mutant. Finally, application of 5-aminolevulinic acid to the atl31/atl6 mutants resulted in recovery to a green phenotype. Taken together, these findings indicate that the 5-aminolevulinic acid biosynthesis step was inhibited through the down-regulation of chlorophyll biosynthesis-related genes in the pale-green leaves of atl31/atl6 mutant.

    DOI: 10.1371/journal.pone.0117662

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  • Phosphorylation of Arabidopsis Ubiquitin Ligase ATL31 Is Critical for Plant Carbon/Nitrogen Nutrient Balance Response and Controls the Stability of 14-3-3 Proteins 査読有り

    Shigetaka Yasuda, Takeo Sato, Shugo Maekawa, Shoki Aoyama, Yoichiro Fukao, Junji Yamaguchi

    JOURNAL OF BIOLOGICAL CHEMISTRY289 ( 22 ) 15179 - 15193   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

    Background: Ubiquitin ligase ATL31 and the target, 14-3-3 proteins, function in plant nutrient response. Results: ATL31 binds to 14-3-3 proteins via phosphorylation of specific residues. These residues are essential for the function of ATL31. Conclusion: ATL31 targets 14-3-3 proteins for degradation in a phosphorylation-dependent manner to regulate nutrient response. Significance: Phosphorylation of ubiquitin ligase ATL31 controls plant nutrient response.
    Ubiquitin ligase plays a fundamental role in regulating multiple cellular events in eukaryotes by fine-tuning the stability and activity of specific target proteins. We have previously shown that ubiquitin ligase ATL31 regulates plant growth in response to nutrient balance between carbon and nitrogen (C/N) in Arabidopsis. Subsequent study demonstrated that ATL31 targets 14-3-3 proteins for ubiquitination and modulates the protein abundance in response to C/N-nutrient status. However, the underlying mechanism for the targeting of ATL31 to 14-3-3 proteins remains unclear. Here, we show that ATL31 interacts with 14-3-3 proteins in a phosphorylation-dependent manner. We identified Thr(209), Ser(247), Ser(270), and Ser(303) as putative 14-3-3 binding sites on ATL31 by motif analysis. Mutation of these Ser/Thr residues to Ala in ATL31 inhibited the interaction with 14-3-3 proteins, as demonstrated by yeast two-hybrid and co-immunoprecipitation analyses. Additionally, we identified in vivo phosphorylation of Thr(209) and Ser(247) on ATL31 by MS analysis. A peptide competition assay showed that the application of synthetic phospho-Thr(209) peptide, but not the corresponding unphosphorylated peptide, suppresses the interaction between ATL31 and 14-3-3 proteins. Moreover, Arabidopsis plants overexpressing mutated ATL31, which could not bind to 14-3-3 proteins, showed accumulation of 14-3-3 proteins and growth arrest in disrupted C/N-nutrient conditions similar to wild-type plants, although overexpression of intact ATL31 resulted in repression of 14-3-3 accumulation and tolerance to the conditions. Together, these results demonstrate that the physiological role of phosphorylation at 14-3-3 binding sites on ATL31 is to modulate the binding ability and stability of 14-3-3 proteins to control plant C/N-nutrient response.

    DOI: 10.1074/jbc.M113.533133

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  • The Carbon/Nitrogen Regulator ARABIDOPSIS TOXICOS EN LEVADURA31 Controls Papilla Formation in Response to Powdery Mildew Fungi Penetration by Interacting with SYNTAXIN OF PLANTS121 in Arabidopsis 査読有り

    Shugo Maekawa, Noriko Inada, Shigetaka Yasuda, Yoichiro Fukao, Masayuki Fujiwara, Takeo Sato, Junji Yamaguchi

    PLANT PHYSIOLOGY164 ( 2 ) 879 - 887   2014年2月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC PLANT BIOLOGISTS  

    The carbon/nitrogen (C/N) balance of plants is not only required for growth and development but also plays an important role in basal immunity. However, the mechanisms that link C/N regulation and basal immunity are poorly understood. We previously demonstrated that the Arabidopsis (Arabidopsis thaliana) Arabidopsis Toxicos en Levadura31 (ATL31) ubiquitin ligase, a regulator of the C/N response, positively regulates the defense response against bacterial pathogens. In this study, we identified the plasma membrane-localized soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor SYNTAXIN OF PLANTS121 (SYP121) as a novel ATL31 interactor. The syp121-1 loss-of-function mutant showed similar hypersensitivity to C/N stress conditions as the atl31 atl6 double mutant. SYP121 is essential for resistance to penetration by powdery mildew fungus and positively regulates the formation of cell wall appositions (papillae) at fungal entry sites. Microscopic analysis demonstrated that ATL31 was specifically localized around papillae. In addition, ATL31 overexpressors showed accelerated papilla formation, enhancing their resistance to penetration by powdery mildew fungus. Together, these data indicate that ATL31 plays an important role in connecting the C/N response with basal immunity by promoting papilla formation through its association with SYP121.

    DOI: 10.1104/pp.113.230995

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  • Proteomics Analysis Reveals a Highly Heterogeneous Proteasome Composition and the Post-translational Regulation of Peptidase Activity under Pathogen Signaling in Plants 査読有り

    Hui H. Sun, Yoichiro Fukao, Sakiko Ishida, Hiroko Yamamoto, Shugo Maekawa, Masayuki Fujiwara, Takeo Sato, Junji Yamaguchi

    JOURNAL OF PROTEOME RESEARCH12 ( 11 ) 5084 - 5095   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER CHEMICAL SOC  

    The proteasome is a large multisubunit complex that plays a crucial role in the removal of damaged or selective ubiquitinated proteins, thereby allowing quality control of cellular proteins and restricted regulation of diverse cellular signaling in eukaryotic cells. Proteasome-dependent protein degradation is involved in almost all aspects of plant growth and responses to environmental stresses including pathogen resistance. Although the molecular mechanism for specifying targets by ubiquitin ligases is well understood, the detailed characterization of the plant proteasome complex remains unclear. One of the most important features of the plant proteasome is that most subunits are encoded by duplicate genes, suggesting the highly heterogeneous composition of this proteasome. Here, we performed affinity purification and a combination of 2-dimensional electrophoresis and mass spectrometry, which identified the detailed composition of paralogous and modified proteins. Moreover, these proteomics approaches revealed that specific subunit composition and proteasome peptidase activity were affected by pathogen-derived MAMPs, flg22 treatment. Interestingly, flg22 treatment did not alter mRNA expression levels of the peptidase genes PBA, PBB1/2, PBE1/2, and total proteasome levels remained unchanged by flg22 as well. These results demonstrate the finely tuned mechanism that regulates proteasome function via putative post-translational modifications in response to environmental stress in plants.

    DOI: 10.1021/pr400630w

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  • The Arabidopsis ubiquitin ligases ATL31 and ATL6 control the defense response as well as the carbon/nitrogen response 査読有り

    Shugo Maekawa, Takeo Sato, Yutaka Asada, Shigetaka Yasuda, Midori Yoshida, Yukako Chiba, Junji Yamaguchi

    PLANT MOLECULAR BIOLOGY79 ( 3 ) 217 - 227   2012年6月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER  

    In higher plants, the metabolism of carbon (C) and nitrogen nutrients (N) is mutually regulated and referred to as the C and N balance (C/N). Plants are thus able to optimize their growth depending on their cellular C/N status. Arabidopsis and encode a RING-type ubiquitin ligases which play a critical role in the C/N status response (Sato et al. in Plant J 60:852-864, 2009). Since many ATL members are involved in the plant defense response, the present study evaluated whether the C/N response regulators ATL31 and ATL6 are involved in defense responses. Our results confirmed that and expression is up-regulated with the microbe-associated molecular patterns elicitors flg22 and chitin as well as with infections with pv. DC3000 ( DC3000). Moreover, transgenic plants overexpressing and displayed increased resistance to DC3000. In accordance with these data, loss of ATL31 and ATL6 function in an double knockout mutant resulted in reduced resistance to DC3000. In addition, the molecular cross-talk between C/N and the defense response was investigated by mining public databases. The analysis identified the transcription factors MYB51 and WRKY33, which are involved in the defense response, and their transcripts levels correlate closely with and . Further study demonstrated that the expression of , and defense marker genes including and were regulated by C/N conditions. Taken together, these results indicate that ATL31 and ATL6 function as key components of both C/N regulation and the defense response in Arabidopsis.

    DOI: 10.1007/s11103-012-9907-0

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  • Arabidopsis RPT2a, 19S Proteasome Subunit, Regulates Gene Silencing via DNA Methylation 査読有り

    Kaori Sako, Yuko Maki, Tomoyuki Kanai, Eriko Kato, Shugo Maekawa, Shigetaka Yasuda, Takeo Sato, Masaaki K. Watahiki, Junji Yamaguchi

    PLOS ONE7 ( 5 ) e37086   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    The ubiquitin/proteasome pathway plays a crucial role in many biological processes. Here we report a novel role for the Arabidopsis 19S proteasome subunit RPT2a in regulating gene activity at the transcriptional level via DNA methylation. Knockout mutation of the RPT2a gene did not alter global protein levels; however, the transcriptional activities of reporter transgenes were severely reduced compared to those in the wild type. This transcriptional gene silencing (TGS) was observed for transgenes under control of either the constitutive CaMV 35S promoter or the cold-inducible RD29A promoter. Bisulfite sequencing analysis revealed that both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant. Moreover, the TGS of transgenes driven by the CaMV 35S promoters was released by treatment with the DNA methylation inhibitor 5-aza-2'-deoxycytidine, but not by application of the inhibitor of histone deacetylase Trichostatin A. Genetic crosses with the DNA methyltransferase met1 single or drm1drm2cmt3 triple mutants also resulted in a release of CaMV 35S transgene TGS in the rpt2a mutant background. Increased methylation was also found at transposon sequences, suggesting that the 19S proteasome containing AtRPT2a negatively regulates TGS at transgenes and at specific endogenous genes through DNA methylation.

    DOI: 10.1371/journal.pone.0037086

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  • Proteomics approach to study metabolic regulation mediated by ubiquitin-proteasome system in Arabidopsis 査読有り

    Yasuda S, Maekawa S, Sato T, Yamaguchi J

    Frontiers of Agriculture Proteome Research   39 - 44   2012年

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  • Sugar-inducible RPT2a, a subunit of 26S proteasome, participates in sugar response in Arabidopsis 査読有り

    Huihui Sun, Kaori Sako, Yuya Suzuki, Shugo Maekawa, Shigetaka Yasuda, Yukako Chiba, Takeo Sato, Junji Yamaguchi

    PLANT BIOTECHNOLOGY29 ( 3 ) 279 - 284   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY  

    The ubiquitin/26S proteasome system (UPS) plays a central role in the degradation of short-lived regulatory proteins that control many cellular events. In this study, the Arabidopsis knockout mutant rpt2a, which contains a defect in the AtRPT2a subunit of the 26S proteasome regulatory particle, showed hypersensitivity to sugars as well as enlarged leaves. When the role of RPT2a in sugar response was examined in further detail it was found that putatively only the AtRPT2a gene of 19S proteasome was markedly transcriptionally promoted by sugar application. Notably, poly-ubiquitinated proteins degraded by the UPS accumulated significantly in rpt2a mutant under 6% sucrose conditions compared to wild type. In addition, the AtRPT2a gene in gin2, a glucose insensitive mutant with a defective glucose-sensing hexokinase, was not upregulated by sugar application, indicating that AtRPT2a is involved in hexokinase-dependent sugar response. Taken together, the above findings indicate that AtRPT2a plays an essential role in the maintenance of proteasome-dependent proteolysis activity in response to sugars.

    DOI: 10.5511/plantbiotechnology.12.0409a

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  • 植物のRING型ユビキチンリガーゼとプロテアソームの機能 査読有り

    前川修吾, 佐古香織, 佐藤長緒

    生化学84 ( 6 ) 416 - 424   2012年

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    担当区分:筆頭著者   記述言語:日本語  

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  • Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis 査読有り

    Takeo Sato, Shugo Maekawa, Shigetaka Yasuda, Yukie Domeki, Kuni Sueyoshi, Masayuki Fujiwara, Yoichiro Fukao, Derek B. Goto, Junji Yamaguchi

    PLANT JOURNAL68 ( 1 ) 137 - 146   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

    The balance between carbon (C) and nitrogen (N) availability is an important determinant for various phases of plant growth; however, the detailed mechanisms regulating the C/N response are not well understood. We previously described two related ubiquitin ligases, ATL31 and ATL6, that function in the C/N response in Arabidopsis thaliana. Here, we used FLAG tag affinity purification and MS analysis to identify proteins targeted by ATL31, and thus likely to be involved in regulating the phase transition checkpoint based on C/N status. This analysis revealed that 14-3-3 proteins were associated with ATL31, and one of these, 14-3-3 chi, was selected for detailed characterization. The interaction between ATL31 and 14-3-3 chi was confirmed by yeast two-hybrid and co-immunoprecipitation analyses. In vitro assays showed that ubiquitination of 14-3-3 chi is catalyzed by ATL31. Degradation of 14-3-3 chi in vivo was shown to be correlated with ATL31 activity, and to occur in a proteasome-dependent manner. Furthermore, 14-3-3 protein accumulation was induced by a shift to high-C/N stress conditions in Arabidopsis seedlings, and this regulated response required both ATL31 and ATL6. It was also shown that over-expression of 14-3-3 chi leads to hypersensitivity of Arabidopsis seedlings to C/N stress conditions. These results indicate that ATL31 targets and ubiquitinates 14-3-3 proteins for degradation via the ubiquitin-proteasome system during the response to cellular C/N status.

    DOI: 10.1111/j.1365-313X.2011.04673.x

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  • Carbon and nitrogen metabolism regulated by the ubiquitin-proteasome system 査読有り

    Takeo Sato, Shugo Maekawa, Shigetaka Yasuda, Junji Yamaguchi

    Plant Signaling and Behavior6 ( 10 ) 1465 - 1468   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Landes Bioscience  

    The ubiquitin-proteasome system (UPS) is a unique protein degradation mechanism conserved in the eukaryotic cell. In addition to the control of protein quality, UPS regulates diverse cellular signal transduction via the fine-tuning of target protein degradation. Protein ubiquitylation and subsequent degradation by the 26S proteasome are involved in almost all aspects of plant growth and development and response to biotic and abiotic stresses. Recent studies reveal that the UPS plays an essential role in adaptation to carbon and nitrogen availability in plants. Here we highlight ubiquitin ligase ATL31 and the homolog ATL6 target 14-3-3 proteins for ubiquitylation to be degraded, which control signaling for carbon and nitrogen metabolisms and C/N balance response. We also give an overview of the UPS function involved in carbon and nitrogen metabolisms. © 2011 Landes Bioscience.

    DOI: 10.4161/psb.6.10.17343

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  • CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings 査読有り

    Takeo Sato, Shugo Maekawa, Shigetaka Yasuda, Yutaka Sonoda, Etsuko Katoh, Takanari Ichikawa, Miki Nakazawa, Motoaki Seki, Kazuo Shinozaki, Minami Matsui, Derek B. Goto, Akira Ikeda, Junji Yamaguchi

    PLANT JOURNAL60 ( 5 ) 852 - 864   2009年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL PUBLISHING, INC  

    P&gt;Plants are able to sense and respond to changes in the balance between carbon (C) and nitrogen (N) metabolite availability, known as the C/N response. During the transition to photoautotrophic growth following germination, growth of seedlings is arrested if a high external C/N ratio is detected. To clarify the mechanisms for C/N sensing and signaling during this transition period, we screened a large collection of FOX transgenic plants, overexpressing full-length cDNAs, for individuals able to continue post-germinative growth under severe C/N stress. One line, cni1-D (carbon/nitrogen insensitive 1-dominant), was shown to have a suppressed sensitivity to C/N conditions at both the physiological and molecular level. The CNI1 cDNA encoded a predicted RING-type ubiquitin ligase previously annotated as ATL31. Overexpression of ATL31 was confirmed to be responsible for the cni1-D phenotype, and a knock-out of this gene resulted in hypersensitivity to C/N conditions during post-germinative growth. The ATL31 protein was confirmed to contain ubiquitin ligase activity using an in vitro assay system. Moreover, removal of this ubiquitin ligase activity from the overexpressed protein resulted in the loss of the mutant phenotype. Taken together, these data demonstrated that CNI1/ATL31 activity is required for the plant C/N response during seedling growth transition.

    DOI: 10.1111/j.1365-313X.2009.04006.x

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担当経験のある科目(授業)

所属学協会

共同研究・競争的資金等の研究

  • 植物におけるリボソームストレスの感知とその情報伝達機構の解析

    日本学術振興会  科学研究費助成事業 基盤研究(C) 

    堀口 吾朗, 古賀 皓之, 前川 修吾

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    2019年4月 - 2022年3月

    課題番号:19K06714

    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

    真核生物はリボソーム生合成異常を監視しそれに対処するためのリボソームストレス応答機構を備えている。我々はシロイヌナズナをモデルに植物のリボソームストレス応答に関わる因子を同定している。RINGタンパク質の一種であるSZK2およびリボソームタンパク質のRPL12の下流にSZK1をはじめとした4種のNAC型転写因子を位置付けている。本研究では、「異常型リボソームタンパク質の存在下では、RPL12によってSZK2が活性化され、下流のNAC型転写因子を活性化することでリボソームストレス応答が誘導される」という仮説を立て、その検証を進めている。
    本年度、SZK2とRPL12Bの機能解析にある程度の進展が見られた。RINGドメインを持つタンパク質はユビキチンリガーゼとして働く場合が多い。In vitroユビキチン化アッセイからSZK2の自己ユビキチン可能が検出された。イースト2ハイブリッド法と共免疫沈降法からはSZK2とRPL12Bの相互作用が検出された。既知のアミノ酸置換型RPL12B変異タンパク質は、SZK2との相互作用能を失っていた。また、タバコ葉での共発現、野生型およびT-DNA挿入を持つszk2-2変異株の細胞抽出物を用いた実験から、RPL12BはSZK2依存的に不安定化することを見出した。興味深いことにSZK2には2種のスプライスバリアントが存在し、そのうちの片方のみがRPL12Bを不安定化した。
    上記の実験と並行して、as2 rpl4dおよびas2 rpl4d szk2のサプレッサー変異株の変異部位の同定を進めた。前者のサプレッサーはリボソームストレス応答が誘導できない変異株、後者はリボソームストレス応答誘導能が回復した変異株である。合計10系統のリシーケンスを行い、szk1やszk2の新規アリルに加え、数種の新規変異遺伝子候補を見出した。

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  • 植物の炭素/窒素制御による病原菌の感染様式に応じた抵抗性獲得機構の評価

    JSPS  科研費ー若手研究 

    前川 修吾

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    2018年4月 - 2021年3月

    担当区分:研究代表者  資金種別:競争的資金

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  • プロテオーム解析による植物の窒素応答を支える分子ネットワークの解明

    JSPS  特別研究員(PD) 

    前川 修吾

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    2015年4月 - 2018年3月

    担当区分:研究代表者  資金種別:競争的資金

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  • 膜局在型植物ユビキチンリガーゼATL31による環境シグナル伝達調節の包括的解明

    日本学術振興会  科学研究費助成事業 基盤研究(B) 

    山口 淳二, 佐藤 長緒, 佐古 香織, 前川 修吾

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    2014年4月 - 2017年3月

    課題番号:26292188

    配分額:17940000円 ( 直接経費:13800000円 、 間接経費:4140000円 )

    シロイヌナズナATL31は膜在型のユビキチンリガーゼであり,栄養素応答だけでなく防御応答にも関与する。ATL31の相互作用因SYP121は,うどんこ病菌の侵入を阻止するためのパピラと呼ばれる植物細胞壁形成の制御因子である。最終的にATL31がSYP121と共にパピラ形成による防御応答に関与することを明らかにした。一方,C/N栄養素応答の鍵制御因子として3種類のCIPKタンパク質を同定した。CIPKはATL31をリン酸化することで安定化し,これがC/N応答に関係する14-3-3タンパク質の分解を制御していることを明らかにした。これはリン酸化を介した栄養素シグナル伝達の新たな仕組みの発見である。

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  • ユビキチンリガーゼATLsによるC/Nシグナル制御機構の解明

    JSPS  特別研究員(DC1) 

    前川 修吾

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    2010年4月 - 2013年3月

    担当区分:研究代表者  資金種別:競争的資金

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  • ユビキチンープロテアソームシステムによる環境ストレス応答制御機構の解明

    NAIST  2009年度 植物科学研究教育推進事業4期生 

    前川 修吾

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    2009年4月 - 2010年3月

    担当区分:研究代表者  資金種別:競争的資金

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社会貢献活動

  • 日本植物学会第78回大会 男女共同参画ランチョンセミナー「Living and working together:若手研究者が直面する壁とその打開策」

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    日本植物学会  2014年 - 2014年

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    種別:セミナー・ワークショップ

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