2023/12/20 更新

写真b

ヤノ コウイチ
矢野 晃一
YANO Koichi
*大学が定期的に情報更新している項目(その他は、researchmapの登録情報を転載)
所属*
理学部
職名*
助教
学内職務経歴*
  • 2022年10月 - 現在 
    理学部   助教
 

研究分野

  • ライフサイエンス / 分子生物学

論文

  • Amino acid residues for specific binding to ssDNA facilitate topological loading of bacterial condensin MukB

    Koichiro Akiyama, Koichi Yano, Hironori Niki

        2023年9月21日

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    出版者・発行元:Cold Spring Harbor Laboratory  

    ABSTRACT

    The bacterial condensin MukB facilitates proper chromosome segregation inEscherichia coli. A portion of the MukB proteins localize at a specific chromosome region, binding to DNA in a non-sequence-specific manner. However, it is unclear how MukB localizes at a particular site without sequence specificity. Like other structural maintenance of chromosome (SMC) proteins, MukB topologically loads onto DNA, and It has an intrinsic property of preferential topological loading onto the single-stranded DNA (ssDNA). We consider it crucial for the localization of a specific region. To investigate the property of MukB, we attempted to identify positively charged amino acid residues responsible for ssDNA binding. We created a series of mutated MukB proteins in which a single positively charged amino acid was replaced with a negatively charged one. The results showed that some substitutions located on the inner surface of the MukB head domain impacted ssDNA-binding activity, leading to deficiencies in cell growth and nucleoid segregation. The efficiency of topological loading onto ssDNA was also decreased when the positive charges were replaced with negative ones. These amino acid residues align with and bind to ssDNA when the MukB dimer secures ssDNA within its ring, thereby likely strengthening the ssDNA-binding ability of MukB.

    DOI: 10.1101/2023.09.21.558748

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  • Profiling a single-stranded DNA region within an rDNA segment that affects the loading of bacterial condensin

    Koichi Yano, Hideki Noguchi, Hironori Niki

    iScience25 ( 12 ) 105504 - 105504   2022年12月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.isci.2022.105504

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  • Profiling a single-stranded DNA region within an rDNA segment that is a loading site for bacterial condensin

    Koichi Yano, Hideki Noguchi, Hironori Niki

        2021年6月18日

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    出版者・発行元:Cold Spring Harbor Laboratory  

    <title>Abstract</title>Bacterial condensin preferentially loads to single-stranded DNA (ssDNA) in vitro and loads onto rDNA in vivo to support proper chromosome compaction. Thus, the actively transcribing rDNA would provide the ssDNA region for the topological loading of bacterial condensin. We attempted to detect the ssDNA region in the <italic>rrnI</italic> gene in situ. Non-denaturing sodium bisulfite treatment catalyzed the conversion of cytosines to thymines via uracils (CT-conversion) at locally melted DNA of a bacterial genome. Using next-generation sequencing, we generated an average of 11,000 reads covering each cytosine on the PCR-amplified rDNA segment to obtain the actual CT-conversion rate. In principle, the CT-conversion rate is an accurate guide to detect the formation of the ssDNA segment. We expected that an increment of the CT-conversion rate would reflect a trend toward ssDNA accumulation at a given site within the rDNA. We detected multiple ssDNA segments throughout the rDNA. The deletion mutations of the rDNA that affect the bacterial-condensin loading hindered the ssDNA formation only at the 100–500 bp segment downstream of the promoter. These data support the idea that the ssDNA segment plays a crucial role as the bacterial condensin-loading site and suggest the mechanism of condensin loading onto rDNA.

    DOI: 10.1101/2021.06.17.448897

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  • In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA

    Koichi Yano, Koichiro Akiyama, Hironori Niki

    Methods in Molecular Biology   181 - 196   2019年5月

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    担当区分:筆頭著者   掲載種別:論文集(書籍)内論文   出版者・発行元:Springer New York  

    DOI: 10.1007/978-1-4939-9520-2_14

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  • Multiple cis-Acting rDNAs Contribute to Nucleoid Separation and Recruit the Bacterial Condensin Smc-ScpAB 査読有り

    Koichi Yano, Hironori Niki

    CELL REPORTS21 ( 5 ) 1347 - 1360   2017年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:CELL PRESS  

    Condensins load onto DNA to organize chromosomes. Smc-ScpAB clearly loads onto the parS sites bound by Spo0J, but other loading site(s) must operate independently of parS. In this study, we asked where and how Smc-ScpAB normally selects its loading site. Our results suggest that rDNA is also a loading site. A pull-down assay revealed that Smc-ScpAB preferentially loads onto rDNA in the wildtype cell and even in a Delta spo0J mutant but not in a Delta smc mutant. Moreover, we showed that deletion mutants of rDNAs cause a defect in nucleoid separation, and at least two rDNAs near oriC are essential for separation. Full-length rDNA, including promoters, is required for loading and nucleoid separation. A synthetic defect by deletions of both rDNA and spo0J resulted in more aberrant nucleoid separation. We propose that a single-stranded segment of DNA that is exposed at highly transcribed rRNA operons would become a target for Smc-ScpAB loading.

    DOI: 10.1016/j.celrep.2017.10.014

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  • In vitro topological loading of bacterial condensin MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA 査読有り

    Hironori Niki, Koichi Yano

    SCIENTIFIC REPORTS6   29469   2016年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Condensin is the major driving force in the segregation of daughter chromosomes in prokaryotes. Core subunits of condensin belong to the SMC protein family, whose members are characterized by a unique ATPase activity and dimers with a V-shaped structure. The V-shaped dimers might close between head domains, forming a ring structure that can encircle DNA. Indeed, cohesin, which is a subfamily of SMC proteins, encircles double-stranded DNA to hold sister chromatids in eukaryotes. However, the question of whether or not condensin encircles the chromosomal DNA remains highly controversial. Here we report that MukB binds topologically to DNA in vitro, and this binding is preferentially single-stranded DNA (ssDNA) rather than double-stranded DNA. The binding of MukB to ssDNA does not require ATP. In fact, thermal energy enhances the binding. The non-SMC subunits MukF and MukE did stimulate the topological binding of MukB, although they hindered DNA-binding of MukB. Recent reports on the distribution of condensin in genomes reveal that actively transcribed genes in yeast and humans are enriched in condensin. In consideration of all these results, we propose that the binding specificity of condensin to chromosome is provided not by the DNA sequence but by the DNA structure, which is ssDNA.

    DOI: 10.1038/srep29469

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  • Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis 査読有り

    Genki Akanuma, Yuka Kazo, Kazumi Tagami, Hirona Hiraoka, Koichi Yano, Shota Suzuki, Ryo Hanai, Hideaki Nanamiya, Yasuyuki Kato-Yamada, Fujio Kawamura

    MICROBIOLOGY-SGM162 ( 3 ) 448 - 458   2016年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOLOGY SOC  

    Ribosome dimers are a translationally inactive form of ribosomes found in Escherichia coli and many other bacterial cells. In this study, we found that the 70S ribosomes of Bacillus subtilis dimerized during the early stationary phase and these dimers remained in the cytoplasm until regrowth was initiated. Ribosome dimerization during the stationary phase required the hpf gene, which encodes a homologue of the E. coli hibernation-promoting factor (Hpf). The expression of hpf was induced at an early stationary phase and its expression was observed throughout the rest of the experimental period, including the entire 6 h of the stationary phase. Ribosome dimerization followed the induction of hpf in WT cells, but the dimerization was impaired in cells harbouring a deletion in the hpf gene. Although the absence of ribosome dimerization in these Hpf-deficient cells did not affect their viability in the stationary phase, their ability to regrow from the stationary phase decreased. Thus, following the transfer of stationary-phase cells to fresh LB medium, Delta hpf mutant cells grew slower than WT cells. This observed lag in growth of Delta hpf cells was probably due to a delay in restoring their translational activity. During regrowth, the abundance of ribosome dimers in WT cells decreased with a concomitant increase in the abundance of 70S ribosomes and growth rate. These results suggest that the ribosome dimers, by providing 70S ribosomes to the cells, play an important role in facilitating rapid and efficient regrowth of cells under nutrient-rich conditions.

    DOI: 10.1099/mic.0.000234

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  • Growth and sporulation defects in Bacillus subtilis mutants with a single rrn operon can be suppressed by amplification of the rrn operon 査読有り

    Koichi Yano, Kenta Masuda, Genki Akanuma, Tetsuya Wada, Takashi Matsumoto, Yuh Shiwa, Taichiro Ishige, Hirofumi Yoshikawa, Hironori Niki, Takashi Inaoka, Fujio Kawamura

    MICROBIOLOGY-SGM162 ( 1 ) 35 - 45   2016年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The genome of Bacillus subtilis strain 168 encodes ten rRNA (rrn) operons. We previously reported that strains with only a single rrn operon had a decreased growth and sporulation frequency. We report here the isolation and characterization of suppressor mutants from seven strains that each have a single rrn operon (rrnO, A, J, 1, E, D or B). The suppressor mutants for strain RIK656 with a single rrnO operon had a higher frequency of larger colonies. These suppressor mutants had not only increased growth rates, but also increased sporulation frequencies and ribosome levels compared to the parental mutant strain RIK656. Quantitative PCR analyses showed that all these suppressor mutants had an increased number of copies of the rrnO operon. Suppressor mutants were also isolated from the six other strains with single rrn operons (rrnA, J, 1, E, D or B). Next generation and capillary sequencing showed that all of the suppressor mutants had tandem repeats of the chromosomal locus containing the remaining rrn operon (amplicon). These amplicons varied in size from approximately 9 to 179 kb. The amplifications were likely to be initiated by illegitimate recombination between non- or micro-homologous sequences, followed by unequal crossing-over during DNA replication. These results are consistent with our previous report that rrn operon copy number has a major role in cellular processes such as cell growth and sporulation.

    DOI: 10.1099/mic.0.000207

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  • Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis 査読有り

    Koichi Yano, Tetsuya Wada, Shota Suzuki, Kazumi Tagami, Takashi Matsumoto, Yuh Shiwa, Taichiro Ishige, Yasuhiro Kawaguchi, Kenta Masuda, Genki Akanuma, Hideaki Nanamiya, Hironori Niki, Hirofumi Yoshikawa, Fujio Kawamura

    MICROBIOLOGY-SGM159   2225 - 2236   2013年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SOC GENERAL MICROBIOLOGY  

    The number of copies of rRNA (rrn) operons in a bacterial genome differs greatly among bacterial species. Here we examined the phenotypic effects of variations in the number of copies of rRNA genes in the genome of Bacillus subtilis by analysis of eight mutant strains constructed to carry from two to nine copies of the rrn operon. We found that a decrease in the number of copies from ten to one increased the doubling time, and decreased the sporulation frequency and motility. The maximum levels for transformation activity were similar among the strains, although the competence development was significantly delayed in the strain with a single rrn operon. Normal sporulation only occurred if more than four copies of the rrn operon were present, although ten copies were needed for vegetative growth after germination of the spores. This behaviour was seen even though the intracellular level of ribosomes was similar among strains with four to ten copies of the rrn operon. Furthermore, ten copies of the rrn operon were needed for the highest swarming activity. We also constructed 21 strains that carried all possible combinations of two copies of the rrn operons, and found that these showed a range of growth rates and sporulation frequencies that all fell between those recorded for strains with one or three copies of the rrn operon. The results suggested that the copy number of the rrn operon has a major influence on cellular processes such as growth rate and sporulation frequency.

    DOI: 10.1099/mic.0.067025-0

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  • rRNA (rrn) operon-engineered Bacillus subtilis as a feasible test organism for antibiotic discovery 査読有り

    Yukinori Tanaka, Hideaki Nanamiya, Koichi Yano, Koji Kakugawa, Fujio Kawamura, Kozo Ochi

    Antimicrobial Agents and Chemotherapy57 ( 4 ) 1948 - 1951   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:4  

    Bacillus subtilis contains 10 rRNA (rrn) operons. We found that rRNA operon-engineered B. subtilis strain RIK543, with only the rrnO operon, is specifically hypersensitive to RNA polymerase inhibitors such as rifamycin SV and rifampin (80-fold and 20-fold, respectively). In pilot screening experiments, we found actinomycete isolates successfully at an incidence of 1.9% (18/945) that produced antibacterials that were detectable only with RIK543 as the test organism. Strain RIK543 may be a feasible test organism for the discovery of novel RNA polymerase inhibitors. Copyright © 2013, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/AAC.02604-12

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  • Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis 査読有り

    Genki Akanuma, Shota Suzuki, Koichi Yano, Hideaki Nanamiya, Yousuke Natori, Eri Namba, Kazuya Watanabe, Kazumi Tagami, Takuya Takeda, Yuka Iizuka, Ako Kobayashi, Morio Ishizuka, Hirofumi Yoshikawa, Fujio Kawamura

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY59 ( 2 ) 105 - 117   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOL RES FOUNDATION  

    We introduced single mutations into the rplC and rpsJ genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium Bacillus subtilis, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45 degrees C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.

    DOI: 10.2323/jgam.59.105

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  • Inactivation of Ribosomal Protein Genes in Bacillus subtilis Reveals Importance of Each Ribosomal Protein for Cell Proliferation and Cell Differentiation 査読有り

    Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura

    JOURNAL OF BACTERIOLOGY194 ( 22 ) 6282 - 6291   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER SOC MICROBIOLOGY  

    Among the 57 genes that encode ribosomal proteins in the genome of Bacillus subtilis, a Gram-positive bacterium, 50 genes were targeted by systematic inactivation. Individual deletion mutants of 16 ribosomal proteins (L1, L9, L15, L22, L23, L28, L29, L32, L33.1, L33.2, L34, L35, L36, S6, S20, and S21) were obtained successfully. In conjunction with previous reports, 22 ribosomal proteins have been shown to be nonessential in B. subtilis, at least for cell proliferation. Although several mutants that harbored a deletion of a ribosomal protein gene did not show any significant differences in any of the phenotypes that were tested, various mutants showed a reduced growth rate and reduced levels of 70S ribosomes compared with the wild type. In addition, severe defects in the sporulation frequency of the Delta rplA (L1) mutant and the motility of the Delta rpsU (S21) mutant were observed. These data provide the first evidence in B. subtilis that L1 and S21 are required for the progression of cellular differentiation.

    DOI: 10.1128/JB.01544-12

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  • Bacillus subtilis RNA polymerase incorporates digoxigenin-labeled nucleotide in vitro 査読有り

    Koichi Yano, Yee Lii Mien, Yoshito Sadaie, Kei Asai

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY57 ( 3 ) 153 - 157   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:MICROBIOL RES FOUNDATION  

    DOI: 10.2323/jgam.57.153

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  • The Genome of Bacillus subtilis Phage SP10: A Comparative Analysis with Phage SPO1 査読有り

    Lii Mien Yee, Takashi Matsumoto, Koichi Yano, Satoshi Matsuoka, Yoshito Sadaie, Hirofumi Yoshikawa, Kei Asai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY75 ( 5 ) 944 - 952   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    A nucleotide sequence of the whole genome of Bacillus subtilis phage SP10 was determined. It was composed of 143,986 bp with 236 putative open reading frames (ORFs). Sixty-five of 236 predicted ORFs showed high similarity to that of SPO1, and the genome organizations of the two phages were similar to each other. SP10 belongs to the Myoviridae family, for which the well-studied phage SPO1 is the representative phage. Hence, we compared SP10 to SPO1. The SP10 genome DNA showed different sensitivity to restriction enzymes than SPO1, due to differences in base modification. According to transcriptional analysis, the gene expression of regulatory network of SP10 was similar to SPO1. It was observed that RNA polymerase containing sigma-A was inactive in directing the host genes but active in directing the phage genes. It appeared that the association of sigma-A with the core enzyme complex of RNA polymerase was strengthened during development.

    DOI: 10.1271/bbb.100921

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  • Heterologous Expression of the Oceanobacillus iheyensis SigW and Its Anti-Protein RsiW in Bacillus subtilis 査読有り

    Koichi Yano, Hiromi Inoue, Hirokazu Mori, Lii Mien Yee, Satoshi Matsuoka, Yoshito Sadaie, Kei Asai

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY75 ( 5 ) 966 - 975   2011年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:TAYLOR & FRANCIS LTD  

    Pairs of the ECF sigma factor and its anti-sigma factor, SigW and RsiW, of Bacillus-related species that inhabit extreme environments were heterologously expressed in B. subtilis. All the RsiWs, membrane proteins, failed to fill their function of repressing cognate SigW activity, despite their close structural similarities. Particularly, uncontrolled expression of Oceanobacillus iheyensis OISigW due to abortive OIRsiW was harmful to B. subtilis. Analysis of revertants of this growth defect and site-directed mutagenesis indicated that the insertion of six and a minimum of three hydrophobic amino acid residues occurring in the transmembrane region allowed OIRsiW to function as anti-OISigW. Subcellular localization of OIRsiW was detected by immunoblot analysis, suggesting that both the wild-type and the mutant form of OIRsiW were localized to the membrane. An appropriate length of a transmembrane region required for proper integration into the membrane after translocation might vary among these Bacillus-related species.

    DOI: 10.1271/bbb.110035

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  • Inhibitory effect of prophage SPβ fragments on phage SP10 ribonucleotide reductase function and its multiplication in Bacillus subtilis. 査読有り

    Yee LM, Matsuoka S, Yano K, Sadaie Y, Asai K

    Genes & genetic systems86 ( 1 ) 7 - 18   2011年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:1  

    DOI: 10.1266/ggs.86.7

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MISC

  • DNA修復因子が司る出芽酵母レトロトランスポゾンのサイレンシング機構

    増本博司, 武藤秀樹, 黒崎陽平, 矢野晃一, 仁木宏典

    日本分子生物学会年会プログラム・要旨集(Web)44th   2021年

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  • バクテリアコンデンシンがロードするrDNAに生じる一本鎖DNA領域の1塩基レベルでの同定

    矢野晃一, 野口英樹, 仁木宏典

    日本分子生物学会年会プログラム・要旨集(Web)42nd   2019年

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  • A new aspect of rRNA genes; involvement in origin resolution by DNA condensation

    Hironori Niki, Koichi Yano

    GENES & GENETIC SYSTEMS90 ( 6 ) 403 - 403   2015年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Molecular genetic analysis of the function of rRNA in Bacillus subtilis

    Koichi Yano, Eri Namba, Rie Sekine, Shota Suzuki, Kazumi Tagami, Fujio Kawamura

    GENES & GENETIC SYSTEMS87 ( 6 ) 410 - 410   2012年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Development of high expression Bacillus subtilis system using a ribosome possessing an altered Shine-Dalgarno sequence

    Takuya Takeda, Koichi Yano, Shota Suzuki, Eri Nanba, Fujio Kawamura

    GENES & GENETIC SYSTEMS87 ( 6 ) 432 - 432   2012年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Analysis of the degradation of rRNA during an early stage of sporulation in Bacillus subtilis

    Kazuya Watanabe, Koichi Yano, Kazumi Tagami, Eri Namba, Fujio Kawamura

    GENES & GENETIC SYSTEMS87 ( 6 ) 432 - 432   2012年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Regulatory for the dimerization of ribosome by a small (p) ppGpp synthetase, YwaC, in Delta relA Delta yjbM Delta ywaC triple mutant of Bacillus subtilis

    Kazumi Tagami, Koichi Yano, Yuka Kazo, Masahiro Hoshiya, Yoshiaki Ohashi, Hideaki Nanamiya, Yasuo Onishi, Fujio Kawamura

    GENES & GENETIC SYSTEMS86 ( 6 ) 419 - 419   2011年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Functional analysis of mutants deleting the 16S rRNA helix 9 in Bacillus subtilis

    Shota Suzuki, Eri Namba, Takuya Takeda, Koichi Yano, Yasuhiko Sekine, Fujio Kawamura

    GENES & GENETIC SYSTEMS86 ( 6 ) 420 - 420   2011年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Comparative analysis of SigW-YbbM system between Bacillus subtilis and Oceanobacillus iheyensis

    Koichi Yano, Kei Asai

    GENES & GENETIC SYSTEMS85 ( 6 ) 424 - 424   2010年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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  • Comparative analysis of extracytoplasmic function sigma factor among genus Bacillus

    Koichi Yano, Hirokazu Mori, Kei Asai

    GENES & GENETIC SYSTEMS84 ( 6 ) 453 - 453   2009年12月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:GENETICS SOC JAPAN  

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書籍等出版物

  • In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA

    矢野晃一( 担当: 共著)

    2019年 

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講演・口頭発表等

  • The cruciform-centered region inside budding yeast Ty1 retrotransposon manages two distinct transcriptional modes: the gene silencing and the activation of Ty1 transcription

    Hiroshi Masumoto, Miki Hanasaki, Hideki Muto, Koichi Yano, Hironori Niki

    Cold Spring Harbor Symposium  2020年10月6日 

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    開催年月日: 2020年10月6日 - 2020年10月9日

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  • レトロトランスポゾンTy1中のDNAのcruciform構造を中心とした遺伝子サイレンシング機構

    鼻崎美紀, 武藤秀樹, 矢野晃一, 仁木宏典, 増本博司

    酵母遺伝学フォーラム  2020年9月7日 

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    開催年月日: 2020年9月7日 - 2020年9月9日

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  • バクテリアコンデンシンがロードするrDNAに生じる一本鎖DNA領域の1塩基レベルでの同定

    矢野晃一, 野口英樹, 仁木宏典

    第42回日本分子生物学会年会  2019年12月3日 

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    記述言語:日本語   会議種別:ポスター発表  

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  • コンデンシンがロードするrDNA領域に生じる一本鎖DNAの1塩基レベルでの検出

    矢野晃一, 野口英樹, 仁木宏典

    第25回DNA複製・組換え・修復ワークショップ  2019年11月9日 

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    開催年月日: 2019年11月9日 - 2019年11月11日

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  • Cis-acting rDNA acts as a loading site for Smc-ScpAB during nucleoid separation in Bacillus subtilis

    Koichi Yano, Hironori Niki

    2017年6月11日 

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    開催年月日: 2017年6月11日 - 2017年6月16日

    記述言語:英語   会議種別:口頭発表(一般)  

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  • Multiple cis-acting rDNAs contribute to nucleoid separation by loading of condensin, Smc-ScpAB

    Koichi Yano, Hironori Niki

    Gordon Research Conferences  2017年5月21日 

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    開催年月日: 2017年5月21日 - 2017年5月26日

    記述言語:英語   会議種別:口頭発表(一般)  

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▼全件表示

共同研究・競争的資金等の研究

  • 一本鎖rDNAを介したコンデンシンの染色体結合機構の解明

    国立遺伝学研究所  科研費(若手研究) 

    矢野晃一

    詳細を見る

    2018年4月 - 2021年3月

    担当区分:研究代表者  資金種別:競争的資金

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