2024/03/20 更新

写真b

ハットリ アツヒコ
服部 淳彦
HATTORI Atsuhiko
*大学が定期的に情報更新している項目(その他は、researchmapの登録情報を転載)
所属*
スポーツウエルネス学部 スポーツウエルネス学科
職名*
特任教授
学位
理学博士 ( 早稲田大学 )
研究キーワード
  • 時間生物学

  • 抗加齢医学

  • 比較内分泌学

  • 分子内分泌学

  • 学内職務経歴*
    • 2023年4月 - 現在 
      スポーツウエルネス学部   スポーツウエルネス学科   特任教授
     

    研究分野

    • ライフサイエンス / 形態、構造

    委員歴

    •  
      日本時間生物学会   評議員

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      団体区分:学協会

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    •  
      日本抗加齢医学会   評議員

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      団体区分:学協会

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      抗加齢内分泌研究会   代表世話人

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      団体区分:学協会

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    論文

    • N1‐Acetyl‐5‐methoxykynuramine, which decreases in the hippocampus with aging, improves long‐term memory via CaMKII/CREB phosphorylation

      Kazuki Watanabe, Yusuke Maruyama, Hikaru Iwashita, Haruyasu Kato, Jun Hirayama, Atsuhiko Hattori

      Journal of Pineal Research76 ( 1 )   2024年1月12日

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Wiley  

      Abstract

      Melatonin is a molecule ubiquitous in nature and involved in several physiological functions. In the brain, melatonin is converted to N1‐acetyl‐N2‐formyl‐5‐methoxykynuramine (AFMK) and then to N1‐acetyl‐5‐methoxykynuramine (AMK), which has been reported to strongly enhance long‐term object memory formation. However, the synthesis of AMK in brain tissues and the underlying mechanisms regarding memory formation remain largely unknown. In the present study, young and old individuals from a melatonin‐producing strain, C3H/He mice, were employed. The amount of AMK in the pineal gland and plasma was very low compared with those of melatonin at night; conversely, in the hippocampus, the amount of AMK was higher than that of melatonin. Indoleamine 2, 3‐dioxygenase (Ido) mRNA was expressed in multiple brain tissues, whereas tryptophan 2,3‐dioxygenase (Tdo) mRNA was expressed only in the hippocampus, and its lysate had melatonin to AFMK conversion activity, which was blocked by the TDO inhibitor. The expression levels of phosphorylated cAMP response element binding protein (CREB) and PSD‐95 in whole hippocampal tissue were significantly increased with AMK treatment. Before increasing in the whole tissue, CREB phosphorylation was significantly enhanced in the nuclear fraction. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, we found that downregulated genes in hippocampus of old C3H/He mice were more enriched for long‐term potentiation (LTP) pathway. Gene set enrichment analysis showed that LTP and neuroactive receptor interaction gene sets were enriched in hippocampus of old mice. In addition, Ido1 and Tdo mRNA expression was significantly decreased in the hippocampus of old mice compared with young mice, and the decrease in Tdo mRNA was more pronounced than Ido1. Furthermore, there was a higher decrease in AMK levels, which was less than 1/10 that of young mice, than in melatonin levels in the hippocampus of old mice. In conclusion, we first demonstrated the Tdo‐related melatonin to AMK metabolism in the hippocampus and suggest a novel mechanism of AMK involved in LTP and memory formation. These results support AMK as a potential therapeutic agent to prevent memory decline.

      DOI: 10.1111/jpi.12934

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    • Melatonin is more effective on bone metabolism when given at early night than during the day in ovariectomized rats. 査読有り

      Yukari Miki, Azusa Seki, Hiroyuki Mishima, Yusuke Maruyama, Kazuki Watanabe, Jingjing Kobayashi-Sun, Isao Kobayashi, Kohei Kuroda, Shion Oshima, Takeru Okamoto, Hajime Matsubara, Ajai K. Srivastav, Yoshiaki Tabuchi, Jun Hirayama, Atsuhiko Hattori, Nobuo Suzuki

      Melatonin Research6   161 - 172   2023年6月

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      掲載種別:研究論文(学術雑誌)  

      DOI: 10.32794/mr112500147

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    • Kynurenine promotes Calcitonin secretion and reduces cortisol in the Japanese flounder Paralichthys olivaceus. 査読有り 国際誌

      Takahiro Ikari, Yukihiro Furusawa, Yoshiaki Tabuchi, Yusuke Maruyama, Atsuhiko Hattori, Yoichiro Kitani, Kenji Toyota, Arata Nagami, Jun Hirayama, Kazuki Watanabe, Atsushi Shigematsu, Muhammad Ahya Rafiuddin, Shouzo Ogiso, Keisuke Fukushi, Kohei Kuroda, Kaito Hatano, Toshio Sekiguchi, Ryotaro Kawashima, Ajai K Srivastav, Takumi Nishiuchi, Akihiro Sakatoku, Masa-Aki Yoshida, Hajime Matsubara, Nobuo Suzuki

      Scientific reports13 ( 1 ) 8700 - 8700   2023年5月29日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Deep ocean water (DOW) exerts positive effects on the growth of marine organisms, suggesting the presence of unknown component(s) that facilitate their aquaculture. We observed that DOW suppressed plasma cortisol (i.e., a stress marker) concentration in Japanese flounder (Paralichthys olivaceus) reared under high-density condition. RNA-sequencing analysis of flounder brains showed that when compared to surface seawater (SSW)-reared fish, DOW-reared fish had lower expression of hypothalamic (i.e., corticotropin-releasing hormone) and pituitary (i.e., proopiomelanocortin, including adrenocorticotropic hormone) hormone-encoding genes. Moreover, DOW-mediated regulation of gene expression was linked to decreased blood cortisol concentration in DOW-reared fish. Our results indicate that DOW activated osteoblasts in fish scales and facilitated the production of Calcitonin, a hypocalcemic hormone that acts as an analgesic. We then provide evidence that the Calcitonin produced is involved in the regulatory network of genes controlling cortisol secretion. In addition, the indole component kynurenine was identified as the component responsible for osteoblast activation in DOW. Furthermore, kynurenine increased plasma Calcitonin concentrations in flounders reared under high-density condition, while it decreased plasma cortisol concentration. Taken together, we propose that kynurenine in DOW exerts a cortisol-reducing effect in flounders by facilitating Calcitonin production by osteoblasts in the scales.

      DOI: 10.1038/s41598-023-35222-4

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    • Nocturnal melatonin increases glucose uptake via insulin-independent action in the goldfish brain

      Kazuki Watanabe, Masaki Nakano, Yusuke Maruyama, Jun Hirayama, Nobuo Suzuki, Atsuhiko Hattori

      Frontiers in Endocrinology14   2023年5月23日

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Frontiers Media SA  

      Melatonin, a neurohormone nocturnally produced by the pineal gland, is known to regulate the circadian rhythm. It has been recently reported that variants of melatonin receptors are associated with an increased risk of hyperglycemia and type 2 diabetes, suggesting that melatonin may be involved in the regulation of glucose homeostasis. Insulin is a key hormone that regulates circulating glucose levels and cellular metabolism after food intake in many tissues, including the brain. Although cells actively uptake glucose even during sleep and without food, little is known regarding the physiological effects of nocturnal melatonin on glucose homeostasis. Therefore, we presume the involvement of melatonin in the diurnal rhythm of glucose metabolism, independent of insulin action after food intake. In the present study, goldfish (Carassius auratus) was used as an animal model, since this species has no insulin-dependent glucose transporter type 4 (GLUT4). We found that in fasted individuals, plasma melatonin levels were significantly higher and insulin levels were significantly lower during the night. Furthermore, glucose uptake in the brain, liver, and muscle tissues also significantly increased at night. After intraperitoneal administration of melatonin, glucose uptake by the brain and liver showed significantly greater increases than in the control group. The administration of melatonin also significantly decreased plasma glucose levels in hyperglycemic goldfish, but failed to alter insulin mRNA expression in Brockmann body and plasma insulin levels. Using an insulin-free medium, we demonstrated that melatonin treatment increased glucose uptake in a dose-dependent manner in primary cell cultures of goldfish brain and liver cells. Moreover, the addition of a melatonin receptor antagonist decreased glucose uptake in hepatocytes, but not in brain cells. Next, treatment with N1-acetyl-5-methoxykynuramine (AMK), a melatonin metabolite in the brain, directly increased glucose uptake in cultured brain cells. Taken together, these findings suggest that melatonin is a possible circadian regulator of glucose homeostasis, whereas insulin acquires its effect on glucose metabolism following food intake.

      DOI: 10.3389/fendo.2023.1173113

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    • Deep ocean water alters the cholesterol and mineral metabolism of squid Todarodes pacificus and suppresses its weight loss

      Kaito Hatano, Masa-Aki Yoshida, Jun Hirayama, Yoichiro Kitani, Atsuhiko Hattori, Shouzo Ogiso, Yukina Watabe, Toshio Sekiguchi, Yoshiaki Tabuchi, Makoto Urata, Kyoko Matsumoto, Akihiro Sakatoku, Ajai K. Srivastav, Kenji Toyota, Hajime Matsubara, Nobuo Suzuki

      Scientific Reports13 ( 1 )   2023年5月10日

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Springer Science and Business Media LLC  

      Abstract

      This study is the first to demonstrate that deep ocean water (DOW) has physiological significant effects on squid. After 36 h of rearing squids, those reared with DOW had significantly higher total and free cholesterol levels and lower alanine transaminase activity in hemolymph as compared with those reared with surface sea water (SSW). SSW rearing also resulted in 6.95% weight loss, while DOW rearing caused only 2.5% weight loss, which might be due to liver metabolism suppression. Furthermore, both monovalent (sodium, chloride, and potassium ions) and divalent (calcium, inorganic phosphorus, and magnesium ions) ions in hemolymph were elevated when reared with DOW compared to those when reared with SSW. A study of genes expressed in the brain revealed that five genes were specifically remarked in DOW rearing. Most altered genes were neuropeptides, including those from vasopressin superfamily. These neuropeptides are involved in cholesterol and/or mineral metabolisms and physiological significant effects on squid. This study is the first report the effects of DOW on cholesterol and mineral metabolism of squid and will contribute to squid aquaculture using DOW.

      DOI: 10.1038/s41598-023-34443-x

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      その他リンク: https://www.nature.com/articles/s41598-023-34443-x

    • Adaptation to the shallow sea floor environment of a species of marine worms, Oligobrachia mashikoi, generally inhabiting deep-sea water. 国際誌

      Shouzo Ogiso, Kazuki Watanabe, Yusuke Maruyama, Hiroshi Miyake, Kaito Hatano, Jun Hirayama, Atsuhiko Hattori, Yukina Watabe, Toshio Sekiguchi, Yoichiro Kitani, Yukihiro Furusawa, Yoshiaki Tabuchi, Hajime Matsubara, Mana Nakagiri, Kenji Toyota, Yuichi Sasayama, Nobuo Suzuki

      Scientific reports13 ( 1 ) 6299 - 6299   2023年4月18日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Beard worms from the family Siboglinidae, are peculiar animals and are known for their symbiotic relationships with sulfur bacteria. Most Siboglinids inhabit the deep-sea floor, thus making difficult to make any observations in situ. One species, Oligobrachia mashikoi, occurs in the shallow depths (24.5 m) of the Sea of Japan. Taking advantage of its shallow-water habitat, the first ecological survey of O. mashikoi was performed over a course of 7 years, which revealed that its tentacle-expanding behavior was dependent on the temperature and illuminance of the sea water. Furthermore, there were significantly more O. mashikoi with expanding tentacles during the nighttime than during the daytime, and the prevention of light eliminated these differences in the number of expending tentacles. These results confirmed that the tentacle-expanding behavior is controlled by environmental light signals. Consistent with this, we identified a gene encoding a photoreceptor molecule, neuropsin, in O. mashikoi, and the expression thereof is dependent on the time of day. We assume that the described behavioral response of O. mashikoi to light signals represent an adaptation to a shallow-water environment within the predominantly deep-sea taxon.

      DOI: 10.1038/s41598-023-33309-6

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    • Physiological consequences of space flight, including abnormal bone metabolism, space radiation injury, and circadian clock dysregulation: Implications of melatonin use and regulation as a countermeasure. 国際誌

      Jun Hirayama, Atsuhiko Hattori, Akihisa Takahashi, Yukihiro Furusawa, Yoshiaki Tabuchi, Masahiro Shibata, Aiko Nagamatsu, Sachiko Yano, Yusuke Maruyama, Hajime Matsubara, Toshio Sekiguchi, Nobuo Suzuki

      Journal of pineal research   e12834   2022年10月6日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Exposure to the space environment induces a number of pathophysiological outcomes in astronauts, including bone demineralization, sleep disorders, circadian clock dysregulation, cardiovascular and metabolic dysfunction, and reduced immune system function. A recent report describing experiments aboard the Space Shuttle mission, STS-132, showed that the level of melatonin, a hormone that provides the biochemical signal of darkness, was decreased during microgravity in an in vitro culture model. Additionally, abnormal lighting conditions in outer space, such as low light intensity in orbital spacecraft and the altered 24-hour light-dark cycles, may result in the dysregulation of melatonin rhythms and the misalignment of the circadian clock from sleep and work schedules in astronauts. Studies on Earth have demonstrated that melatonin regulates various physiological functions including bone metabolism. These data suggest that the abnormal regulation of melatonin in outer space may contribute to pathophysiological conditions of astronauts. In addition, experiments with high-linear energy transfer radiation, a ground-based model of space radiation, showed that melatonin may serve as a protectant against space radiation. Gene expression profiling using an in vitro culture model exposed to space flight during the STS-132 mission, showed that space radiation alters the expression of DNA repair and oxidative stress response genes, indicating that melatonin counteracts the expression of these genes responsive to space radiation to promote cell survival. These findings implicate the use of exogenous melatonin and the regulation of endogenous melatonin as countermeasures for the physiological consequences of space flight. This article is protected by copyright. All rights reserved.

      DOI: 10.1111/jpi.12834

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    • Functional analysis of a matrix peptide involved in calcification of the exoskeleton of the kuruma prawn, Marsupenaeus japonicus

      Aika Sekimoto, Tsuyoshi Ohira, Atsushi Shigematsu, Takuji Okumura, Miyuki Mekuchi, Kenji Toyota, Hiroyuki Mishima, Ryoya Kawamura, Kaito Hatano, Umi Kawago, Yoichiro Kitani, Toshio Sekiguchi, Thumronk Amornsakun, Jun Hirayama, Atsuhiko Hattori, Hajime Matsubara, Nobuo Suzuki

      Aquaculture   738437 - 738437   2022年6月

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

      DOI: 10.1016/j.aquaculture.2022.738437

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    • Osteoclastic and Osteoblastic Responses to Hypergravity and Microgravity: Analysis Using Goldfish Scales as a Bone Model

      Tatsuki Yamamoto, Mika Ikegame, Yukihiro Furusawa, Yoshiaki Tabuchi, Kaito Hatano, Kazuki Watanabe, Umi Kawago, Jun Hirayama, Sachiko Yano, Toshio Sekiguchi, Kei-ichiro Kitamura, Masato Endo, Arata Nagami, Hajime Matsubara, Yusuke Maruyama, Atsuhiko Hattori, Nobuo Suzuki

      Zoological Science39 ( 4 )   2022年4月5日

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Zoological Society of Japan  

      DOI: 10.2108/zs210107

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    • Hydroxylated benzo[c]phenanthrene metabolites cause osteoblast apoptosis and skeletal abnormalities in fish

      Nobuo Suzuki, Masato Honda, Masayuki Sato, Shuhei Yoshitake, Kimi Kawabe, Yoshiaki Tabuchi, Toshiki Omote, Toshio Sekiguchi, Yukihiro Furusawa, Akira Toriba, Ning Tang, Yohei Shimasaki, Edward G. Nagato, Lulu Zhang, Ajai K. Srivastav, Thumronk Amornsakun, Yoichiro Kitani, Hajime Matsubara, Takashi Yazawa, Jun Hirayama, Atsuhiko Hattori, Yuji Oshima, Kazuichi Hayakawa

      Ecotoxicology and Environmental Safety234   113401 - 113401   2022年4月

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

      DOI: 10.1016/j.ecoenv.2022.113401

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    • Activation of RANKL-producing cells under simulated microgravity with a three-dimensional clinostat in regenerating goldfish scales

      Tatsuki Yamamoto, Mika Ikegame, Kohei Kuroda, Jingjing Kobayashi-Sun, Jun Hirayama, Isao Kobayashi, Ryoya Kawamura, Masato Endo, Yoshiaki Tabuchi, Yukihiro Furusawa, Koji Yachiguchi, Toshio Sekiguchi, Hajime Matsubara, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki

      Biological Sciences in Space36   9 - 14   2022年

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:Japanese Society for Biological Sciences in Space  

      DOI: 10.2187/bss.36.9

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    • Oral administration of melatonin increases plasma calcium and magnesium and improves bone metabolism in aged male mice

      Junko Igarashi-Migitaka, Yusuke Maruyama, Azusa Seki, Jun Hirayama, Atsuko Kamijo-Ikemori, Kazuaki Hirata, Ryoya Kawamura, Hajime Matsubara, Ajai K Srivastav, Yoshiaki Tabuchi, Hiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki

      Melatonin Research4 ( 4 ) 581 - 591   2021年12月31日

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      掲載種別:研究論文(学術雑誌)   出版者・発行元:ST Bio-life  

      We previously reported that the oral administration of melatonin from 4 to 20 months to male mice improved femoral bone strength and bone density during the aging. Additionally, melatonin receptor, MT2, was immunologically detected in both osteoblasts and osteoclasts of the mouse femoral bone. Thus, melatonin can act on both osteoblasts and osteoclasts to maintain bone strength during the aging process. Here, we analyzed plasma calcium (Ca2+), magnesium (Mg2+), and inorganic phosphorus ([PO4]3-) in 20-month-old male mice with or without administration melatonin (15-20 mg/kg/day) in drinking water. We found that plasma Ca2+ and Mg2+ levels in melatonin-treated mice increased significantly as compared with control mice. In [PO4]3-, melatonin administration tended to increase its plasma level, but did not reach statistical significance. The potential association between these divalent ions and metabolism markers of femoral bone was also examined. In the femoral diaphysis, the plasma Ca2+ and Mg2+ concentrations were positively correlated with periosteal and endosteal circumference which were significantly associated with the Strength Strain Index. Therefore, melatonin treatment enlarged femoral diaphysis and enhanced bone strength by increasing mineral depositions. In addition, the plasma melatonin levels were significantly positive correlation with total bone density and critical thickness in the femoral diaphysis. Since we had not observed the primary trabecular bone and osteoclasts in 20-month-old mice previously, it is suggested that plasma Ca2+ and Mg2+ are not elevated due to bone resorption. The increased plasma Ca2+ and Mg2+ by melatonin may originate from the intestinal absorption of these ions since melatonin binds to the vitamin D3 receptor, its activation is known to promote the intestinal absorption of Ca2+.

      DOI: 10.32794/mr112500113

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    • Association between Dietary Melatonin Intake and Total and Cause-Specific Mortality in Japanese Adults in the Takayama Study 査読有り

      Chisato Nagata, Keiko Wada, Michiyo Yamakawa, Yuma Nakashima, Sachi Koda, Takahiro Uji, Sakiko Onuma, Shino Oba, Yuuske Maruyama, Atsuhiko Hattori

      American Journal of Epidemiology   2021年8月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1093/aje/kwab213

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    • Effects of antioxidant in adjunct with periodontal therapy in patients with type 2 diabetes: A systematic review and meta-analysis

      Koji Mizutani, Prima Buranasin, Risako Mikami, Kohei Takeda, Daisuke Kido, Kazuki Watanabe, Shu Takemura, Keita Nakagawa, Hiromi Kominato, Natsumi Saito, Atsuhiko Hattori, Takanori Iwata

      Antioxdants10 ( 8 ) e1304   2021年8月

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      記述言語:英語  

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    • Glyoxal-induced formation of advancedglycation end-products in type 1 collagen decreases both its strength and flexibilityi n vitro 査読有り

      Kei-ichiro Kitamura, Jun Hirayama, Yoshiaki Tabuchi, Takao Minami, Hajime Matsubara, Atsuhiko Hattori, Nobuo Suzuki

      Journal of Diabetes Investigation   2021年2月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1111/jdi.13528

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    • In Vivo Suppression of Osteoclastic and Osteoblastic Activities of Goldfish Scales in Water Containing Cadmium

      Nobuo Suzuki, Umi Kawago, Masato Honda, Ajai K. Srivastav, Thumronk Amornsakun, Kyoko Matsumoto, Jun Hirayama, Hajime Matsubara, Nobuaki Shimizu, Toshio Sekiguchi, Yuichi Sasayama, Yoshiaki Tabuchi, Atsuhiko Hattori, Yohei Shimasaki, Yuji Oshima

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY66 ( 2 ) 199 - 203   2021年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS  

      The fish scales are well known as a major source of internal calcium storage. Therefore, we developed an original assay system using goldfish scales in which both osteoclasts (bone resorption cells) and osteoblasts (bone formation cells) coexist. In our bioassay system, we utilized the activities of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as respective indicators of each activity in osteoclasts and osteoblasts. Using this bioassay system, the influence of cadmium chloride (CdCl2) on osteoclastic and osteoblastic activities in the scales of goldfish was examined in an in vivo experiment. Goldfish were kept in tap water containing CdCl2 (10(-7) M) for 2 days. TRAP activity in the scales of goldfish decreased with 2 days of exposure to CdCl2. This inhibitory effect for osteoclasts continued even at 4 days of exposure to CdCl2. In the case of osteoblasts, CdCl2 inhibited ALP activity in the goldfish scales at 4 days after exposure, although ALP activity in the goldfish scales had not changed after 2 days of exposure to CdCl2. In in vitro cultured goldfish scales, we previously reported that ALP activity decreased after exposures of 64 and 96 hrs, although their activities did not change after 6, 18, and 36 hrs. These results were supported by our in vivo experiment. This is the first report to indicate that both osteoclasts and osteoblasts in fish were suppressed by cadmium (Cd) treatments in vivo. Considering both in vivo and in vitro experiments, we concluded that Cd inhibited both osteoclastic and osteoblastic activities in goldfish. This suggests that Cd leads to a disturbed calcium metabolism and then induces bone anomalies.

      DOI: 10.15017/4486551

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    • The melatonin metabolite N1-acetyl-5-methoxykynuramine facilitates long-term object memory in young and aging mice. 査読有り

      Hikaru Iwashita, Yukihisa Matsumoto, Yusuke Maruyama, Kazuki Watanabe, Atsuhiko Chiba, Atsuhiko Hattori

      J Pineal Res70 ( 1 ) e12703 - e12703   2021年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Melatonin (MEL) has been reported to enhance cognitive processes, making it a potential treatment for cognitive decline. However, the role of MEL's metabolites, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and N1-acetyl-5-methoxykynuramine (AMK), in these effects are unknown. The current study directly investigated the acute effects of systemic MEL, AFMK, and AMK on novel object recognition. We also analyzed MEL, AFMK, and AMK levels in hippocampus and temporal lobe containing the perirhinal cortex following systemic MEL and AMK treatment. AMK administered post-training had a more potent effect on object memory than MEL and AFMK. AMK was also able to rescue age-associated declines in memory impairments when object memory was tested up to 4 days following training. Results from administering AMK at varying times around the training trial and the metabolism time course in brain tissue suggest that AMK's memory-enhancing effects reflect memory consolidation. Furthermore, inhibiting the MEL-to-AMK metabolic pathway disrupted object memory at 24 hours post-training, suggesting that endogenous AMK might play an important role in long-term memory formation. This is the first study to report that AMK facilitates long-term object memory performance in mice, and that MEL crosses the blood-brain barrier and is immediately converted to AMK in brain tissue. Overall, these results support AMK as a potential therapeutic agent to improve or prevent memory decline.

      DOI: 10.1111/jpi.12703

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      その他リンク: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jpi.12703

    • Expression of sclerostin in the regenerating scales of goldfish and its increase under microgravity during space flight. 査読有り

      Tatsuki Yamamoto, Mika Ikegame, Jun Hirayama, Kei-Ichiro Kitamura, Yoshiaki Tabuchi, Yukihiro Furusawa, Toshio Sekiguchi, Masato Endo, Hiroyuki Mishima, Azusa Seki, Sachiko Yano, Hajime Matsubara, Atsuhiko Hattori, Nobuo Suzuki

      Biomed Res41 ( 6 ) 279 - 288   2020年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Osteocytes, osteoblasts (bone-forming cells), and osteoclasts (bone-resorbing cells) are the primary types of cells that regulate bone metabolism in mammals. Sclerostin produced in bone cells activates osteoclasts, inhibiting bone formation; excess production of sclerostin, therefore, leads to the loss of bone mass. Fish scales have been reported to have morphological and functional similarities to mammalian bones, making them a useful experimental system for analyzing vertebrate bone metabolism in vitro. However, whether fish scales contain cells producing sclerostin and/or osteocytes has not been determined. The current study demonstrated, for the first time, that sclerostin-containing cells exist in goldfish scales. Analysis of the distribution and shape of sclerostin-expressing cells provided evidence that osteoblasts produce sclerostin in goldfish scales. Furthermore, our results found that osteocyte-like cells exist in goldfish scales, which also produce sclerostin. Finally, we demonstrated that microgravity in outer space increased the level of sclerostin in the scales of goldfish, a finding suggesting that the induction of sclerostin is the mechanism underlying the activation of osteoclasts under microgravity.

      DOI: 10.2220/biomedres.41.279

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    • Porphyromonas gingivalis impairs glucose uptake in skeletal muscle associated with altering gut microbiota 国際誌

      Watanabe Kazuki, Katagiri Sayaka, Takahashi Hirokazu, Sasaki Naoki, Maekawa Shogo, Komazaki Rina, Hatasa Masahiro, Kitajima Yoichiro, Maruyama Yusuke, Shiba Takahiko, Komatsu Keiji, Ohsugi Yujin, Tanaka Kenichi, Matsuzawa Ayumi, Hirota Tomomitsu, Tohara Haruka, Eguchi Yuichiro, Anzai Keizo, Hattori Atsuhiko, Iwata Takanori

      FASEB JOURNAL35 ( 2 ) e21171   2020年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1096/fj.202001158R

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    • De novo transcriptome analysis and gene expression profiling of fish scales isolated from Carassius auratus during space flight: Impact of melatonin on gene expression in response to space radiation. 査読有り 国際誌

      Yukihiro Furusawa, Tatsuki Yamamoto, Atsuhiko Hattori, Nobuo Suzuki, Jun Hirayama, Toshio Sekiguchi, Yoshiaki Tabuchi

      Mol Med Rep22 ( 4 ) 2627 - 2636   2020年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Astronauts are inevitably exposed to two major risks during space flight, microgravity and radiation. Exposure to microgravity has been discovered to lead to rapid and vigorous bone loss due to elevated osteoclastic activity. In addition, long‑term exposure to low‑dose‑rate space radiation was identified to promote DNA damage accumulation that triggered chronic inflammation, resulting in an increased risk for bone marrow suppression and carcinogenesis. In our previous study, melatonin, a hormone known to regulate the sleep‑wake cycle, upregulated calcitonin expression levels and downregulated receptor activator of nuclear factor‑κB ligand expression levels, leading to improved osteoclastic activity in a fish scale model. These results indicated that melatonin may represent a potential drug or lead compound for the prevention of bone loss under microgravity conditions. However, it is unclear whether melatonin affects the biological response induced by space radiation. The aim of the present study was to evaluate the effect of melatonin on the expression levels of genes responsive to space radiation. In the present study, to support the previous data regarding de novo transcriptome analysis of goldfish scales, a detailed and improved experimental method (e.g., PCR duplicate removal followed by de novo assembly, global normalization and calculation of statistical significance) was applied for the analysis. In addition, the transcriptome data were analyzed via global normalization, functional categorization and gene network construction to determine the impact of melatonin on gene expression levels in irradiated fish scales cultured in space. The results of the present study demonstrated that melatonin treatment counteracted microgravity‑ and radiation‑induced alterations in the expression levels of genes associated with DNA replication, DNA repair, proliferation, cell death and survival. Thus, it was concluded that melatonin may promote cell survival and ensure normal cell proliferation in cells exposed to space radiation.

      DOI: 10.3892/mmr.2020.11363

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    • Melatonin suppresses both osteoblast and osteoclast differentiation through repression of epidermal Erk signaling in the zebrafish scale. 査読有り 国際誌

      Jingjing Kobayashi-Sun, Nobuo Suzuki, Atsuhiko Hattori, Masaaki Yamaguchi, Isao Kobayashi

      Biochem Biophys Res Commun530 ( 4 ) 644 - 650   2020年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Melatonin has been implicated in the regulation of bone metabolism; however, the molecular mechanisms underlying its involvement in fracture healing are still obscure. We previously developed an in vivo fracture healing model using the scale of a double-transgenic zebrafish, trap:GFP; osterix:mCherry, which labels osteoclasts and osteoblasts with GFP and mCherry, respectively. Here we show using this model that melatonin inhibits both osteoblast and osteoclast differentiation under fracture stress through the repression of Erk signaling in epidermal cells of the scale. Melatonin treatment resulted in reduced numbers of both osteoblasts and osteoclasts in the fractured scale. Immunochemistry analysis revealed that Erk signals in epidermal cells, which express melatonin receptors, were greatly enhanced in response to fracture stress, but this enhancement was blocked by melatonin treatment. Moreover, inhibition of Erk signaling phenocopied the effects of melatonin treatment in the fractured scale. Collectively, these data suggest that the activation of epidermal Erk signaling is required for both osteoblast and osteoclast differentiation in the early stage of fracture healing, and melatonin suppresses epidermal Erk signaling, leading to impaired fracture healing.

      DOI: 10.1016/j.bbrc.2020.07.075

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    • Oral administration of melatonin contained in drinking water increased bone strength in naturally aged mice. 査読有り 国際誌

      Junko Igarashi-Migitaka, Azusa Seki, Mika Ikegame, Masato Honda, Toshio Sekiguchi, Hiroyuki Mishima, Nobuaki Shimizu, Hajime Matsubara, Ajai K Srivastav, Jun Hirayama, Yusuke Maruyama, Atsuko Kamijo-Ikemori, Kazuaki Hirata, Atsuhiko Hattori, Nobuo Suzuki

      Acta Histochem122 ( 6 ) 151596 - 151596   2020年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Melatonin has recently been found to be a possible new regulator of bone metabolism. However, the influence of melatonin in natural age-related osteoporosis has not been fully elucidated yet, although there have been some reports regarding postmenopausal osteoporosis with melatonin treatments. The present study investigated the effects of long-term melatonin administration during the aging process on bone metabolism. Using quantitative computed tomography methods, we found that the total bone density of both the femur metaphysis and diaphysis decreased significantly in 20-month-old male mice. In the metaphysis, both trabecular bone mass and Polar-Strength Strain Index (SSI), which is an index of bone strength, decreased significantly. Judging from bone histomorphometry analysis, trabecular bone in 20-month-old male mice decreases significantly with age and is small and sparse, as compared to that of 4-month-old male mice. Loss of trabecular bone is one possible cause of loss of bone strength in the femoral bone. In the metaphysis, the melatonin administration group had significantly higher trabecular bone density than the non-administration group. The Polar-SSI, cortical area, and periosteal circumference in the diaphysis was also significantly higher with melatonin treatments. Since the melatonin receptor, MT2, was detected in both osteoblasts and osteoclasts of the femoral bone of male mice, we expect that melatonin acts on osteoblasts and osteoclasts to maintain the bone strength of the diaphysis and metaphysis. Thus, melatonin is a potential drug for natural age-related osteoporosis.

      DOI: 10.1016/j.acthis.2020.151596

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    • Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale. 査読有り 国際誌

      Jingjing Kobayashi-Sun, Shiori Yamamori, Mao Kondo, Junpei Kuroda, Mika Ikegame, Nobuo Suzuki, Kei-Ichiro Kitamura, Atsuhiko Hattori, Masaaki Yamaguchi, Isao Kobayashi

      Communications biology3 ( 1 ) 190 - 190   2020年4月23日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Differentiation of osteoclasts (OCs) from hematopoietic cells requires cellular interaction with osteoblasts (OBs). Due to the difficulty of live-imaging in the bone, however, the cellular and molecular mechanisms underlying intercellular communication involved in OC differentiation are still elusive. Here, we develop a fracture healing model using the scale of trap:GFP; osterix:mCherry transgenic zebrafish to visualize the interaction between OCs and OBs. Transplantation assays followed by flow cytometric analysis reveal that most trap:GFPhigh OCs in the fractured scale are detected in the osterix:mCherry+ fraction because of uptake of OB-derived extracellular vesicles (EVs). In vivo live-imaging shows that immature OCs actively interact with osterix:mCherry+ OBs and engulf EVs prior to convergence at the fracture site. In vitro cell culture assays show that OB-derived EVs promote OC differentiation via Rankl signaling. Collectively, these data suggest that EV-mediated intercellular communication with OBs plays an important role in the differentiation of OCs in bone tissue.

      DOI: 10.1038/s42003-020-0925-1

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    • Influence of Benz[a]anthracene on Bone Metabolism and on Liver Metabolism in Nibbler Fish, Girella punctata. 査読有り 国際誌

      Mohamed I Zanaty, Niina Sawada, Yoichiro Kitani, Hossam F Nassar, Hamada M Mahmoud, Kazuichi Hayakawa, Toshio Sekiguchi, Shouzo Ogiso, Yoshiaki Tabuchi, Makoto Urata, Hajime Matsubara, Yutaka Takeuchi, Atsuhiko Hattori, Ajai K Srivastav, Thumronk Amornsakun, Nobuo Suzuki

      International journal of environmental research and public health17 ( 4 )   2020年2月21日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[a]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution.

      DOI: 10.3390/ijerph17041391

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    • The Use of Chemical Compounds to Identify the Regulatory Mechanisms of Vertebrate Circadian Clocks. 査読有り 国際誌

      Yoshimi Okamoto-Uchida, Akari Nishimura, Junko Izawa, Atsuhiko Hattori, Nobuo Suzuki, Jun Hirayama

      Current drug targets21 ( 5 ) 425 - 432   2020年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Circadian clocks are intrinsic, time-tracking processes that confer a survival advantage on an organism. Under natural conditions, they follow approximately a 24-h day, modulated by environmental time cues, such as light, to maximize an organism's physiological efficiency. The exact timing of this rhythm is established by cell-autonomous oscillators called cellular clocks, which are controlled by transcription-translation negative feedback loops. Studies of cell-based systems and wholeanimal models have utilized a pharmacological approach in which chemical compounds are used to identify molecular mechanisms capable of establishing and maintaining cellular clocks, such as posttranslational modifications of cellular clock regulators, chromatin remodeling of cellular clock target genes' promoters, and stability control of cellular clock components. In addition, studies with chemical compounds have contributed to the characterization of light-signaling pathways and their impact on the cellular clock. Here, the use of chemical compounds to study the molecular, cellular, and behavioral aspects of the vertebrate circadian clock system is described.

      DOI: 10.2174/1389450120666190926143120

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    • Melatonin is a potential drug for the prevention of bone loss during space flight 査読有り

      Mika Ikegame, Atsuhiko Hattori, Makoto J. Tabata, Kei ichiro Kitamura, Yoshiaki Tabuchi, Yukihiro Furusawa, Yusuke Maruyama, Tatsuki Yamamoto, Toshio Sekiguchi, Risa Matsuoka, Taizo Hanmoto, Takahiro Ikari, Masato Endo, Katsunori Omori, Masaki Nakano, Sayaka Yashima, Sadakazu Ejiri, Toshiki Taya, Hiroshi Nakashima, Nobuaki Shimizu, Masahisa Nakamura, Takashi Kondo, Kazuichi Hayakawa, Ichiro Takasaki, Atsushi Kaminishi, Ryosuke Akatsuka, Yuichi Sasayama, Takumi Nishiuchi, Masayuki Nara, Hachiro Iseki, Vishwajit S. Chowdhury, Shigehito Wada, Kenichi Ijiri, Toshio Takeuchi, Tohru Suzuki, Hironori Ando, Kouhei Matsuda, Masanori Somei, Hiroyuki Mishima, Yuko Mikuni-Takagaki, Hisayuki Funahashi, Akihisa Takahashi, Yoshinari Watanabe, Masahiro Maeda, Hideaki Uchida, Akio Hayashi, Akira Kambegawa, Azusa Seki, Sachiko Yano, Toru Shimazu, Hiromi Suzuki, Jun Hirayama, Nobuo Suzuki

      Journal of Pineal Research67 ( 3 )   2019年10月1日

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      掲載種別:研究論文(学術雑誌)  

      © 2019 The Authors. Journal of Pineal Research Published by John Wiley & Sons Ltd Astronauts experience osteoporosis-like loss of bone mass because of microgravity conditions during space flight. To prevent bone loss, they need a riskless and antiresorptive drug. Melatonin is reported to suppress osteoclast function. However, no studies have examined the effects of melatonin on bone metabolism under microgravity conditions. We used goldfish scales as a bone model of coexisting osteoclasts and osteoblasts and demonstrated that mRNA expression level of acetylserotonin O-methyltransferase, an enzyme essential for melatonin synthesis, decreased significantly under microgravity. During space flight, microgravity stimulated osteoclastic activity and significantly increased gene expression for osteoclast differentiation and activation. Melatonin treatment significantly stimulated Calcitonin (an osteoclast-inhibiting hormone) mRNA expression and decreased the mRNA expression of receptor activator of nuclear factor κB ligand (a promoter of osteoclastogenesis), which coincided with suppressed gene expression levels for osteoclast functions. This is the first study to report the inhibitory effect of melatonin on osteoclastic activation by microgravity. We also observed a novel action pathway of melatonin on osteoclasts via an increase in CALCITONIN secretion. Melatonin could be the source of a potential novel drug to prevent bone loss during space flight.

      DOI: 10.1111/jpi.12594

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    • Suppression of Blue Light at Night Ameliorates Metabolic Abnormalities by Controlling Circadian Rhythms 査読有り 国際誌

      Norihiro Nagai, Masahiko Ayaki, Tatsuo Yanagawa, Atsuhiko Hattori, Kazuno Negishi, Takuro Mori, Takahiro J. Nakamura, Kazuo Tsubota

      Invest Ophthalmol Vis Sci60 ( 12 ) 3786 - 3793   2019年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1167/iovs.19-27195

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    • Development of a system for measuring calcitonin in the stingray Dasyatis akajei (a cartilaginous fish): the possible involvement of stingray calcitonin in gonadal development 査読有り

      Takashi Takagi, Toshio Sekiguchi, Yuichi Sasayama, Atsuhiko Hattori, Takushi X. Watanabe, Yoichiro Kitani, Yoshiaki Tabuchi, Hajime Matsubara, Ajai K. Srivastav, Tran Ngoc Hai, Thumronk Amornsakun, Nobuo Suzuki

      International Aquatic Research11 ( 3 ) 267 - 276   2019年9月1日

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      掲載種別:研究論文(学術雑誌)  

      © 2019, The Author(s). To elucidate the physiological role of calcitonin (CT) in stingrays (cartilaginous fish), an enzyme-linked immunosorbent assay (ELISA) system using a specific antibody against stingray CT has been developed. Synthetic stingray CT was subcutaneously injected into mice four times—once every 2 weeks—together with an adjuvant. We purified the IgG antibody fraction using the protein A affinity chromatography from collected antiserum. Evaluating the antibody titer, we found the antibody’s optimum dilution ratio to be 600 times. Competitive ELISA has been developed using the antibody diluted 600 times. Our antibody did not cross-react with teleost CTs and muscle extraction, but cross-reacted with stingray plasma and the extract of the ultimobranchial gland, the secretary organ of stingray CT. Using this ELISA, we measured the plasma CT level in stingrays and examined its correlation with several mineral concentrations. Plasma CT did not show significant correlation to calcium, magnesium, inorganic phosphorus, sodium, chlorine, or urea, although there was a correlation among the factors involved in osmoregulation, such as sodium, chlorine, and urea. On the other hand, plasma CT was significantly correlated to body weight and length. Furthermore, there was a significant correlation between plasma CT and gonad weight. Since plasma CT was correlated with the weight of liver, which is involved in the synthesis of egg yolk protein, we examined the influence of 17β-estradiol (E2) on CT secretion. After E2 injection, the plasma CT level increased significantly. This is the first study to demonstrate that E2 induced plasma CT secretion in cartilaginous fish.

      DOI: 10.1007/s40071-019-00236-0

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    • Post-translational Modifications are Required for Circadian Clock Regulation in Vertebrates. 査読有り 国際誌

      Yoshimi Okamoto-Uchida, Junko Izawa, Akari Nishimura, Atsuhiko Hattori, Nobuo Suzuki, Jun Hirayama

      Current genomics20 ( 5 ) 332 - 339   2019年8月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Circadian clocks are intrinsic, time-tracking systems that bestow upon organisms a survival advantage. Under natural conditions, organisms are trained to follow a 24-h cycle under environmental time cues such as light to maximize their physiological efficiency. The exact timing of this rhythm is established via cell-autonomous oscillators called cellular clocks, which are controlled by transcription/translation-based negative feedback loops. Studies using cell-based systems and genetic techniques have identified the molecular mechanisms that establish and maintain cellular clocks. One such mechanism, known as post-translational modification, regulates several aspects of these cellular clock components, including their stability, subcellular localization, transcriptional activity, and interaction with other proteins and signaling pathways. In addition, these mechanisms contribute to the integration of external signals into the cellular clock machinery. Here, we describe the post-translational modifications of cellular clock regulators that regulate circadian clocks in vertebrates.

      DOI: 10.2174/1389202919666191014094349

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    • Suppressive effect of melatonin on osteoclast function via osteocyte calcitonin 査読有り

      Masaki Nakano, Mika Ikegame, Junko Igarashi-Migitaka, Yusuke Maruyama, Nobuo Suzuki, Atsuhiko Hattori

      The Journal of endocrinology242 ( 2 ) 13 - 23   2019年8月1日

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      掲載種別:研究論文(学術雑誌)  

      Many studies have investigated the actions of melatonin on osteoblasts and osteoclasts. However, the underlying mechanisms, especially regarding osteocyte function, remain largely unknown. Therefore, this study aimed to clarify the underlying mechanisms of melatonin action on bone tissue via osteocyte function. Chick calvariae were employed as a model. In ovo injection of melatonin (5, 50 and 500 µg) dose-dependently decreased the mRNA expression levels of cathepsin K and matrix metalloproteinase 9 (MMP9) in chick calvariae without affecting the expression levels of receptor activator of NF-κB ligand or osteoprotegerin. Surprisingly enough, the expression of calcitonin mRNA in chick calvariae was significantly raised. After 3 days of in vitro treatment of melatonin (10-7 and 10-5 M) on newly hatched chick calvariae, both calcitonin mRNA expression in calvariae and the concentration of calcitonin in cultured medium were augmented in a dose-dependent manner, coincident with the decreased mRNA expression levels of cathepsin K and MMP9. Immunohistochemical analyses revealed expression of melatonin receptors and calcitonin by osteocytes buried in bone matrix. Moreover, the mRNA expression levels of melatonin receptors, calcitonin and sclerostin (a marker of osteocyte), were strongly and positively correlated. In conclusion, we demonstrated the expression of melatonin receptors and calcitonin expression in osteocytes for the first time and suggest a new mechanism underlying the suppressive effect of melatonin on osteoclasts via upregulation of calcitonin secretion by osteocytes.

      DOI: 10.1530/JOE-18-0707

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    • Effect of mats with “A Distinctive 4-Layer 3 -Dimensional Structure” on sleep quality and nocturnal blood glucose: A crossover trial. 査読有り

      Mari Ogura, Atsuhiko Hattori, Masayuki Yagi, Wakako Takabe, Takuto Nonomura, Yoji Shimura, Midori Ando, Yoshikazu Yonei

      Glycative Stress Research6 ( 1 )   2019年3月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.24659/gsr.6.1_049

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    • Circadian production of melatonin in cartilage modifies rhythmic gene expression. 査読有り

      Fu S, Kuwahara M, Uchida Y, Koudo S, Hayashi D, Shimomura Y, Takagaki A, Nishida T, Maruyama Y, Ikegame M, Hattori A, Kubota S, Hattori T

      Journal of Endocrinology241 ( 2 ) 161 - 173   2019年3月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1530/JOE-19-0022

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    • Influence of Polluted Sea Water in the Red Sea on the Osteoclasts and Osteoblasts of Goldfish, Carassius auratus

      Mohamed Zanaty, Hossam F. Nassar, Hamada M. Mahmoud, Fagr Kh Abdel-Gawad, Masayuki Sato, Koji Yachiguchi, Nobuaki Shimizu, Kazuichi Hayakawa, Atsuhiko Hattori, Koki Mukai, Yohei Shimasaki, Yuji Oshima, Nobuo Suzuki

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY64 ( 2 ) 287 - 291   2019年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS  

      To analyze the influences of environmental pollutants and bioactive substances on fish bone metabolism, we have developed an original in vitro bioassay using goldfish scales that have osteoclasts, osteoblasts, and bone matrix. We used tartrate-resistant acid phosphatase (TRAP) for osteoclasts and alkaline phosphatase (ALP) for osteoblasts as respective markers. Using this assay system, we have reported the influences of heavy metals such as cadmium (Cd) and mercury (Hg) on fish bone metabolism. In the case of Cd, we found that its concentration (even at 10(-13) M) influenced osteoclastic activity in goldfish scales only at 6 hrs of incubation. Thus, in the present study, we examined the effects of polluted seawater on osteoclastic and osteoblastic activities with this scale in vitro bioassay. Polluted seawater was collected from the Suez Gulf site on the Red Sea. Polluted seawater was added into culture medium at dilution rates of 50,100, and 500 times and incubated with the goldfish scales for 6 hrs. Subsequently, the influences of polluted seawater on TRAP and ALP activities were compared with that of artificial seawater as a non-polluted seawater. As a result, TRAP activity was significantly suppressed by the polluted seawater samples diluted at least 500 times; in contrast, ALP activity did not show any change. This response was similar to the response of Cd and Hg. As heavy metal contamination of the Suez Gulf site has been reported, there is a high possibility that heavy metals are contained in the seawater. Considering our data together with environmental pollution in Egypt that has been reported until now, we should conduct a heavy metal risk assessment to protect the ecosystem in the Red Sea.

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    • The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish. 査読有り 国際誌

      Hirayama J, Alifu Y, Hamabe R, Yamaguchi S, Tomita J, Maruyama Y, Asaoka Y, Nakahama KI, Tamaru T, Takamatsu K, Takamatsu N, Hattori A, Nishina S, Azuma N, Kawahara A, Kume K, Nishina H

      Scientific Reports9 ( 1 ) 196 - 196   2019年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1038/s41598-018-37879-8

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    • RANKL, Ephrin-Eph and Wnt10b are key intercellular communication molecules regulating bone remodeling in autologous transplanted goldfish scales 査読有り

      Yuya Tazaki, Kayo Sugitani, Kazuhiro Ogai, Isao Kobayashi, Haruki Kawasaki, Takafumi Aoyama, Nobuo Suzuki, Yoshiaki Tabuchi, Atsuhiko Hattori, Kei ichiro Kitamura

      Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology225   46 - 58   2018年11月

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      掲載種別:研究論文(学術雑誌)  

      © 2018 Elsevier Inc. This study aimed to investigate the precise data of gene expression, functions, and chronological relationships amongst communication molecules involved in the bone remodeling process with an in vivo model using autologous transplanted scales of goldfish. Autotransplantation of methanol-fixed cell-free scales triggers scale resorption and regeneration, as well as helps elucidate the process of bone remodeling. We investigated osteoclastic markers, osteoblastic markers, and gene expressions of communicating molecules (RANKL, ephrinB2, EphB4, EphA4, Wnt10b) by qPCR, in situ hybridization for Wnt10b, and immunohistochemistry for EphrinB2 and EphA4 proteins to elucidate the bone remodeling process. Furthermore, functional inhibition experiments for the signaling of ephrinB2/Eph, ephrin/EphA4, and Wnt10b using specific antibodies, revealed that these proteins are involved in key signaling pathways promoting normal bone remodeling. Our data suggests that the remodeling process comprises of two successive phases. In the first absorption phase, differentiation of osteoclast progenitors by RANKL is followed by the bone absorption by mature, active osteoclasts, with the simultaneous induction of osteoblast progenitors by multinucleated osteoclast-derived Wnt10b, and proliferation of osteoblast precursors by ehprinB2/EphB4 signaling. Subsequently, during the second formation phase, termination of bone resorption by synergistic cooperation occurs, with downregulation of RANKL expression in activated osteoblasts and Ephrin/EphA4-mediated mutual inhibition between neighboring multinucleated osteoclasts, along with simultaneous activation of osteoblasts via forward and reverse EphrinB2/EphB4 signaling between neighboring osteoblasts. In addition, the present study shows that autologous transplantation of methanol-fixed cell-free scale is an ideal in vivo model to study bone remodeling.

      DOI: 10.1016/j.cbpa.2018.06.011

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    • α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish 査読有り

      Takahiro Ikari, Yuki Kobayashi, Yoichiro Kitani, Toshio Sekiguchi, Masato Endo, Akira Kambegawa, Kiyoshi Asahina, Atsuhiko Hattori, Yoshiaki Tabuchi, Thumronk Amornsakun, Kanta Mizusawa, Akiyoshi Takahashi, Nobuo Suzuki

      International Aquatic Research10 ( 3 ) 283 - 292   2018年9月1日

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      掲載種別:研究論文(学術雑誌)  

      © 2018, The Author(s). The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies were removed to allow the regeneration of scales under anesthesia. Thereafter, the influences of α-MSH injection (low dose: 0.1 μg/g body weight; high dose: 1 μg/g body weight) on plasma calcitonin (calcium-regulating hormone) and the calcium content of the scales were investigated. Ten days after removing the scales, we measured the plasma calcitonin and calcium content of both regenerating scales on the left side and ontogenic scales on the right side. At both doses of α-MSH injection, plasma calcitonin concentrations in the α-MSH-treated group were significantly higher than those in the control group. The mRNA expressions of α-MSH-receptors were detected in the ultimobranchial glands (secretory organ of calcitonin), indicating that α-MSH directly functions in ultimobranchial glands and promotes calcitonin secretion. Furthermore, we found that the calcium content of regenerating scales in α-MSH-treated goldfish was higher than that in control goldfish, while the calcium content of ontogenic scales on the right side was significantly decreased by α-MSH injection. There was a significant co-relationship between plasma calcitonin and the calcium content of regenerating scales. The mRNA expression of calcitonin receptors in regenerating scales was remarkably higher than that in ontogenic scales. These results imply that calcitonin functions to promote scale regeneration resulting from the inhibition of bone resorption because calcitonin suppresses osteoclastic activity. Thus, we are the first to demonstrate the interaction between α-MSH and calcitonin in teleosts.

      DOI: 10.1007/s40071-018-0206-5

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    • Effect of melatonin intake on postprandial blood glucose in the breakfast 査読有り

      Mari Ogura, Fuka Okuda, Atsuhiko Hattori, Wakako Takabe, Masayuki Yagi, Yoshikazu Yonei

      Glycative Stress Research5 ( 2 )   2018年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

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    • Sequencing and expression analysis of calcitonin receptor in the scales of goldfish (Carassius auratus) 査読有り

      Ikari, T, Sekiguchi, T, Urata, M, Furusawa, Y, Ikegame, M, Kinoshita, Y, Kitamura, K, Nakabayashi, I, Horita, M, Tabuchi, Y, Hattori, A, Srivastav, A.K, Suzuki, N

      International Journal of Zoological Investigations4   1 - 10   2018年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

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    • α-Melanocyte-stimulating hormone promotes bone resorption resulting from increased osteoblastic and osteoclastic activities in goldfish. 査読有り 国際誌

      Hidenori Ishizu, Toshio Sekiguchi, Takahiro Ikari, Kei-Ichiro Kitamura, Yoichiro Kitani, Masato Endo, Makoto Urata, Yasuko Kinoshita, Atsuhiko Hattori, Ajai K Srivastav, Hiroyuki Mishima, Kanta Mizusawa, Akiyoshi Takahashi, Nobuo Suzuki

      General and comparative endocrinology262   99 - 105   2018年6月1日

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      We examined the effects of α-melanocyte-stimulating hormone (α-MSH) on bone metabolism using regenerating goldfish scales. Normally developed scales on the bodies of goldfish were removed to allow the regeneration of scales under anesthesia. Thereafter, the influence of α-MSH on the regeneration of goldfish scales was investigated in vivo. In brief, α-MSH was injected at a low dose (0.1 μg/g body weight) or a high dose (1 μg/g body weight) into goldfish every other day. Ten days after removing the scales, we collected regenerating scales and analyzed osteoblastic and osteoclastic activities as respective marker enzyme (alkaline phosphatase for osteoblasts, tartrate-resistant acid phosphatase for osteoclasts) activity in the regenerating scales as well as plasma calcium levels. At both doses, osteoblastic and osteoclastic activities in the regenerating scales increased significantly. Plasma calcium concentrations in the α-MSH-treated group (high doses) were significantly higher than those in the control group. Next, in vitro experiments were performed to confirm the results of in vivo experiments. In the cultured regenerating scales, osteoblastic and osteoclastic activities significantly increased with α-MSH (10-7 and 10-6 M) treatment. In addition, real-time PCR analysis indicated that osteoclastogenesis in α-MSH-treated scales was induced by the receptor activator of the NF-κB/receptor activator of the NF-κB ligand/osteoprotegerin pathway. Furthermore, we found that α-MSH receptors (melanocortin receptors 4 and 5) were detected in the regenerating scales. Thus, in teleosts, we are the first to demonstrate that α-MSH functions in bone metabolism and promotes bone resorption via melatonin receptors 4 and/or 5.

      DOI: 10.1016/j.ygcen.2018.03.020

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    • 新しいメラニン重合評価系を用いたプラセンタにおける美白有効成分の同定 招待有り

      服部淳彦

      日本胎盤臨床医学会研究要覧 ( 22 ) 81 - 96   2018年5月

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      記述言語:日本語  

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    • 睡眠とアンチエイジング メラトニンの老化抑制効果 査読有り

      服部 淳彦

      Anti-aging Science9 ( 2 ) 80 - 80   2017年12月

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      記述言語:日本語   出版者・発行元:(株)メディカルレビュー社  

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    • Immunoreactive Calcitonin Cells in the Nervous System of Polychaete Perinereis aibuhitensis 査読有り

      Yoichi Kase, Shouzo Ogisoi, Takahiro Ikari, Toshio Sekiguchi, Yuichi Sasayama, Yoichiro Kitani, Yohei Shimasaki, Yuji Oshima, Akira Kambegawa, Yoshiaki Tabuchi, Atsuhiko Hattori, Nobuo Suzuki

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY62 ( 2 ) 381 - 385   2017年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS  

      We examined immunoreactive calcitonin (iCT) cells in the nervous system of the polychaete Perinereis aibuhitensis using immunohistochemical methods. We found the most iCT cells (53-70 cells) in the cerebral ganglion. We also detected iCT cells (4-6 cells) in the subpharyngeal ganglion. Furthermore, it was noted that iCT cells were present bilaterally in each segment of the ventral nerve cord. These results suggest that iCT cells play some functional roles in the nervous system. Next, the molecular weight (MW) of the iCT substance was examined using the western blotting method. Cerebral ganglia were collected from 200 individuals. These ganglia were homogenized and centrifuged. The separated supernatants were treated with 66% acetone and then dialyzed to remove low MW substances (less than 2,000) at 4 degrees C. After lyophilization, the sample was reconstituted with 1 M acetic acid and then fractionated with an ultrafiltration membrane system into MWs of 3,000 to 10,000. Thereafter, the specimen was separated by SDS-polyacrylamide gel electrophoresis and analyzed by western blotting with anti-salmon calcitonin (CT) antiserum. Our results indicated that the MW of the iCT substance was close to that of teleost fish CT (3.5 kDa). The annelid Capitella teleta has two genes encoding CT-like peptides. This suggests that the iCT substance in P. aibuhitensis includes amino acid residues similar to fish CT and belongs to the CT family.

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    • Sardine procalcitonin amino-terminal cleavage peptide has a different action from calcitonin and promotes osteoblastic activity in the scales of goldfish 査読有り

      Yoichi Kase, Takahiro Ikari, Toshio Sekiguchi, Masayuki Sato, Shouzo Ogiso, Tsuyoshi Kawada, Shin Matsubara, Honoo Satake, Yuichi Sasayama, Masato Endo, Kei-ichiro Kitamura, Atsuhiko Hattori, Takushi X. Watanabe, Yusuke Maruyama, Yoshinari Watanabe, Hisayuki Funahashi, Akira Kambegawa, Nobuo Suzuki

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY211   77 - 83   2017年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      The nucleotide sequence of a sardine preprocalcitonin precursor has been determined from their ultimobranchial glands in the present study. From our analysis of this sequence, we found that sardine procalcitonin was composed of procalcitonin amino-terminal cleavage peptide (N-proCT) (53 amino acids), CT (32 amino acids), and procalcitonin carboxyl-terminal cleavage peptide (C-proCT) (18 amino acids). As compared with C-proCT, N-proCT has been highly conserved among teleosts, reptiles, and birds, which suggests that N-proCT has some bioactivities. Therefore, both sardine N-proCT and sardine CT were synthesized, and their bioactivities for osteoblasts and osteoclasts were examined using our assay system with goldfish scales that consisted of osteoblasts and osteoclasts. As a result, sardine N-proCT (10(-7) M) activated osteoblastic marker enzyme activity, while sardine CT did not change. On the other hand, sardine CT (10(-9) to 10(-7) M) suppressed osteoclastic marker enzyme activity, although sardine N-proCT did not influence enzyme activity. Furthermore, the mRNA expressions of osteoblastic markers such as type 1 collagen and osteocalcin were also promoted by sardine N-proCT (10(-7) M) treatment; however, sardine CT did not influence their expressions. The osteoblastic effects of N-proCT lack agreement. In the present study, we can evaluate exactly the action for osteoblasts because our scale assay system is very sensitive and it is a co-culture system for osteoblasts and osteoclasts with calcified bone matrix. Both CT and N-proCT seem to influence osteoblasts and osteoclasts and promote bone formation by different actions in teleosts.

      DOI: 10.1016/j.cbpa.2017.06.007

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    • Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctata 査読有り

      Masayuki Sato, Koji Yachiguchi, Keiichi Motohashi, Yukio Yaguchi, Yoshiaki Tabuchi, Yoichiro Kitani, Takahiro Ikari, Shouzo Ogiso, Toshio Sekiguchi, Tran Ngoc Hai, Do Thi Thanh Huong, Nguyen Viet Hoang, Makoto Urata, Hiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki

      FISHERIES SCIENCE83 ( 4 ) 543 - 550   2017年7月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER JAPAN KK  

      The influence of sodium fluoride (NaF) on calcium metabolism was examined in nibbler fish (marine teleosts). Two days after the administration of NaF (5 mu g/g of body weight) (around 10(-4) M in fish), we showed that plasma calcium levels significantly decreased in NaF-treated nibbler fish. In addition, we detected fluoride in the treated scales by use of a scanning electron microscope with an energy-dispersive X-ray microanalysis, indicating that NaF directly affects their scales. Therefore, the influence of NaF on osteoblasts and osteoclasts in the scales was examined. In the scales of NaF-injected nibbler fish, tartrate-resistant acid phosphatase (TRAP) (osteoclastic marker enzyme) decreased, although alkaline phosphatase (osteoblastic marker enzyme) was activated. To confirm the effect of NaF on osteoclasts, furthermore, the mRNA expressions of osteoclastic markers (matrix metalloproteinase-9 and TRAP) were decreased significantly 2 days after incubation. In barred knifejaws, plasma calcium levels decreased as they did in nibbler fish. Therefore, NaF functions in both osteoblasts and osteoclasts and then influences calcium metabolism in marine fish. In the marine environment, high levels of fluoride (1.2-1.5 mg F-/l) (around 10(-5)-10(-4) M) are present in seawater. It is probable that teleosts living in seawater efficiently use fluoride to regulate their blood calcium levels.

      DOI: 10.1007/s12562-017-1086-0

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    • Benz[a]anthracene decreases plasma calcium levels resulting from influence of scale osteoclastic and osteoblastic activities in goldfish. 査読有り

      Suzuki, N, Nakano, J, Kawabe, K, Toriba, A, Hayakawa, K, Tang, N, Sekiguchi, T, Tabuchi, Y, Ikegame, M, Shimizu, N, Mishima, H, Hattori, A, Srivastav, A.K, Kitani, Y

      International Journal of Zoological Investigations3   72 - 81   2017年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

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    • Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures 査読有り

      Toshio Sekiguchi, Akira Shiraishi, Honoo Satake, Kenji Kuwasako, Hiroki Takahashi, Masayuki Sato, Makoto Urata, Shuichi Wada, Masato Endo, Takahiro Ikari, Atsuhiko Hattori, Ajai K. Srivastav, Nobuo Suzuki

      GENERAL AND COMPARATIVE ENDOCRINOLOGY246   294 - 300   2017年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      Calcitonin (CT) is a hormone that decreases serum calcium level by suppressing osteoclastic activity in the vertebrate bone. In vertebrates, the structure-function relationship of CTs has been studied extensively. We recently identified three CT superfamily peptides, Bf-CTFP1 to 3, and clarified the molecular and functional characteristics of their receptor and receptor activity-modifying protein in amphioxus, Branchiostoma floridae. However, the CT activity of Bf-CTFPs has yet to be investigated. In the present study, a functional analysis of Bf-CTFPs was performed using goldfish scales having both osteoclasts and osteoblasts. All Bf-CTFPs suppressed osteoclastic activity via a goldfish CT receptor. Although the primary amino acid sequences of the Bf-CTFPs showed low sequence similarity to vertebrate CTs, Bf-CTFP1 to 3 share three amino acids, Thr(25), Thr(27), and Pro(32)-NH2, that are required for receptor binding, with salmon CT. Moreover, homology model analysis revealed that the Bf-CTFPs form alpha-helical structures. The alpha-helical position and length of Bf-CTFP1 and 2 were conserved with those of a highly potent ligand, teleost CT. Interestingly, the composition of the alpha-helix of Bf-CTFP3 differed from those of teleost CT, despite that the action of Bf-CTFP3 on goldfish scales was the same as that of Bf-CTFP1 and 2. Collectively, the present study provides new insights into the structure-function relationship of CT and its functional evolution in chordates. (C) 2017 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.ygcen.2017.01.004

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    • Large-scale integration in tablet screens for blue-light reduction with optimized color: The effects on sleep, sleepiness, and ocular parameters 査読有り

      Masahiko Ayaki, Atsuhiko Hattori, Yusuke Maruyama, Kazuo Tsubota, Kazuno Negishi

      Cogent Biology   2017年2月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1080/23312025.2017.1294550

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    • 生体リズム同調因子メラトニンが象牙芽細胞、象牙質の構造及び石灰化機構に及ぼす影響 査読有り

      三島 弘幸, 武内 昇平, 田辺 咲貴, 服部 淳彦, 鈴木 信雄, 筧 光夫, 松本 敬, 池亀 美華, 武市 和彦, 見明 康雄

      日本再生歯科医学会誌14 ( 1 ) 5 - 17   2017年2月

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      記述言語:日本語   掲載種別:研究論文(学術雑誌)  

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    • キンギョ鱗における多核破骨細胞形成に関する形態学的研究:筋肉内自家移植鱗を用いた解析 査読有り

      池亀美香, 村楚淑恵, 国定勇希, 服部淳彦, 鈴木信雄, 山本敏男

      岡山歯学会雑誌35 ( 2 ) 37 - 42   2017年1月

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      記述言語:日本語   掲載種別:研究論文(大学,研究機関等紀要)  

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    • Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone. 査読有り

      Taizo Hanmoto, Yoshiaki Tabuchi, Mika Ikegame, Takashi Kondo, Kei-Ichiro Kitamura, Masato Endo, Isao Kobayashi, Hiroyuki Mishima, Toshio Sekiguchi, Makoto Urata, Azusa Seki, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki

      Biomedical research (Tokyo, Japan)38 ( 1 ) 71 - 77   2017年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes' mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.

      DOI: 10.2220/biomedres.38.71

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    • Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales 査読有り

      Kei-ichiro Kitamura, Tadashi Andoh, Wakana Okesaku, Yuya Tazaki, Kazuhiro Ogai, Kayo Sugitani, Isao Kobayashi, Nobuo Suzuki, Wenxi Chen, Mika Ikegame, Atsuhiko Hattori

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY203   152 - 158   2017年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      Increased risk of fracture associated with type 2 diabetes has been a topic of recent concern. Fracture rislc is related to a decrease in bone strength, which can be affected by bone metabolism and the quality of the bone. To investigate the cause of the increased fracture rate in patients with diabetes through analyses of bone metabolism and bone matrix protein properties, we used goldfish scales as a bone model for hyperglycemia. Using the scales of seven alloxan-treated and seven vehicle-treated control goldfish, we assessed bone metabolism by analyzing the activity of marker enzymes and mRNA expression of marker genes, and we measured the change in molecular weight of scale matrix proteins with SDS-PAGE. After only a 2-week exposure to hyperglycemia, the molecular weight of alpha- and beta-fractions of bone matrix collagen proteins changed incrementally in the regenerating scales of hyperglycemic goldfish compared with those of euglycemic goldfish. In addition, the relative ratio of the gamma-fraction significantly increased, and a delta-fraction appeared after adding glyceraldehyde a candidate for the formation of advanced glycation end products in diabetes to isolated type 1 collagen in vitro. The enzymatic activity and mRNA expression of osteoblast and osteoclast markers were not significantly different between hyperglycemic and euglycemic goldfish scales. These results indicate that hyperglycemia is likely to affect bone quality through glycation of matrix collagen from an early stage of hyperglycemia. Therefore, non-enzymatic glycation of collagen fibers in bone matrix may lead to the deterioration of bone quality from the onset of diabetes. (C) 2016 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.cbpa.2016.09.010

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    • Sodium fluoride induces hypercalcemia resulting from the upregulation of both osteoblastic and osteoclastic activities in goldfish, Carassius auratus 査読有り

      Masayuki Sato, Taizo Hanmoto, Koji Yachiguchi, Yoshiaki Tabuchi, Takashi Kondo, Masato Endo, Yoichiro Kitani, Toshio Sekiguchi, Makoto Urata, Tran Ngoc Hai, Ajai K. Srivastav, Hiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY189   54 - 60   2016年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      The influence of sodium fluoride (NaF) on calcium metabolism was examined in goldfish (fresh water teleost). At 2 days after administration of NaF (500 ng/g body weight; 5 pg/g body weight) (around 10(-5) to 10(-4)M in goldfish), we indicated that plasma calcium levels upregulated in both doses of NaF-treated goldfish. To examine the mechanism of hypercalcemia by NaF treatments, therefore, direct effects of NaF on osteoblasts and osteoclasts in goldfish were investigated by an original assay system using teleost scale which has osteoblasts, osteoclasts and bone matrix. Alkaline phosphatase activity in the scales increased with the treatment of NaF (10(-6) and 10(-5) M) during 6 h of incubation. Also, tartrate-resistant acid phosphatase activity increased after exposure to NaF (10(-5) M) at the 6 h of incubation. To investigate the osteoclastic activation, the mRNA expression of osteoclastogenesis related factors were examined. The receptor activator of the nuclear factor-kappa B ligand (RANKL) which is known as a factor for osteoclastogenesis, increased in the NaF-treated scales after 6 h of incubation. The ratio of RANKL/osteoprotegerin (osteoclastogenesis inhibitory factor) significantly increased after 6 h of incubation. Resulting from the increase of RANKL mRNA level, the expression of transcription-regulating factors was significantly increased. Furthermore, the expression of functional genes, cathepsin K and matrix metalloproteinase-9 mRNA, was significantly increased. In our knowledge, this is the first report concerning the effects of NaF on osteoblasts and osteoclasts in teleosts. We concluded that NaF influences calcium metabolism via osteoclastic activation in goldfish. (C) 2016 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.cbpc.2016.07.006

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    • Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation. 査読有り

      Mika Ikegame, Yoshiaki Tabuchi, Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Takashi Kondo, Toshio Yamamoto

      Biomed. Res.37 ( 2 ) 117 - 126   2016年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:バイオメディカルリサーチプレス  

      Mechanical stress promotes osteoblast proliferation and differentiation from mesenchymal stem cells (MSCs). Although numerous growth factors and cytokines are known to regulate this process, information regarding the differentiation of mechanically stimulated osteoblasts from MSCs in in vivo microenvironment is limited. To determine the significant factors involved in this process, we performed a global analysis of differentially expressed genes, in response to tensile stress, in the mouse cranial suture wherein osteoblasts differentiate from MSCs. We found that the gene expression levels of several components involved in bone morphogenetic protein, Wnt, and epithelial growth factor signalings were elevated with tensile stress. Moreover gene expression of some extracellular matrices (ECMs), such as cysteine rich protein 61 (Cyr61)/CCN1 and galectin-9, were upregulated. These ECMs have the ability to modulate the activities of cytokines and are known as matricellular proteins. Cyr61/CCN1 expression was prominently increased in the fibroblastic cells and preosteoblasts in the suture. Thus, for the first time we demonstrated the mechanical stimulation of Cyr61/CCN1 expression in osteogenic cells in an ex vivo system. These results suggest the importance of matricellular proteins along with the cytokine-mediated signaling for the mechanical regulation of MSC proliferation and differentiation into osteoblastic cell lineage in vivo.

      DOI: 10.2220/biomedres.37.117

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      その他リンク: http://search.jamas.or.jp/link/ui/2016362875

    • Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus 査読有り

      Nobuo Suzuki, Masayuki Sato, Hossam F. Nassar, Fagr Kh. Abdel-Gawad, Samah M. Bassem, Koji Yachiguchi, Yoshiaki Tabuchi, Masato Endo, Toshio Sekiguchi, Makoto Urata, Atsuhiko Hattori, Hiroyuki Mishima, Youhei Shimasaki, Yuji Oshima, Chun-Sang Hong, Fumiya Makino, Ning Tang, Akira Toriba, Kazuichi Hayakawa

      ZOOLOGICAL SCIENCE33 ( 4 ) 407 - 413   2016年8月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-kappa B ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts.

      DOI: 10.2108/zs150211

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    • Is vaccenic acid (18:1t n-7) associated with an increased incidence of hip fracture? An explanation for the calcium paradox 査読有り

      Kei Hamazaki, Nobuo Suzuki, Kei-ichiro Kitamura, Atsuhiko Hattori, Tetsuro Nagasawa, Miho Itomura, Tomohito Hamazaki

      PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS109   8 - 12   2016年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCI LTD  

      High calcium intake may increase hip fracture (HF) incidence. This phenomenon, known as the calcium paradox, might be explained by vaccenic acid (18:1t n-7, VA), the highly specific trans fatty acid (TFA) present in dairy products. First, we ecologically investigated the relationship between 18:1 TFA intake and HF incidence using data from 12 to 13 European countries collected before 2000; then we measured the effects of VA and elaidic acid (18:1t n-9, EA) on osteoblasts from goldfish scales (tissues very similar to mammalian bone), with alkaline phosphatase as a marker; and finally we measured the effect of VA on mRNA expression in the scales for the major bone proteins type I collagen and osteocalcin. HF incidence was significantly correlated with 18:1 TFA intake in men (r=0.57) and women (r=0.65). Incubation with 1 mu mol/L VA and EA for 48 h significantly decreased alkaline phosphatase activity by 25% and 21%, respectively. Incubation of scales with 10 mu mol/L VA for 48 h significantly decreased mRNA expression for type I collagen and osteocalcin (by about 50%). In conclusion, VA may be causatively related to HF and could explain the calcium paradox. It may be prudent to reduce 18:1 TFA intake, irrespective of trans positions, to prevent HF. (C) 2016 Elsevier Ltd. All rights reserved.

      DOI: 10.1016/j.plefa.2016.04.001

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    • Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound 査読有り

      Nobuo Suzuki, Taizo Hanmoto, Sachiko Yano, Yukihiro Furusawa, Mika Ikegame, Yoshiaki Tabuchi, Takashi Kondo, Kei-ichiro Kitamura, Masato Endo, Toshio Yamamoto, Toshio Sekiguchi, Makoto Urata, Yuko Mikuni-Takagaki, Atsuhiko Hattori

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY195   26 - 31   2016年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 h of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 h of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in osteoclasts using the TUNEL (TdT-mediated dUTP nick end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 h of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure. (C) 2016 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.cbpa.2016.01.022

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    • Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation 査読有り

      Ikegame Mika, Tabuchi Yoshiaki, Furusawa Yukihiro, Kawai Mariko, Hattori Atsuhiko, Kondo Takashi, Yamamoto Toshio

      BIOMEDICAL RESEARCH-TOKYO37 ( 2 ) 117 - 126   2016年

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      記述言語:英語   出版者・発行元:バイオメディカルリサーチプレス  

      Mechanical stress promotes osteoblast proliferation and differentiation from mesenchymal stem cells (MSCs). Although numerous growth factors and cytokines are known to regulate this process, information regarding the differentiation of mechanically stimulated osteoblasts from MSCs in in vivo microenvironment is limited. To determine the significant factors involved in this process, we performed a global analysis of differentially expressed genes, in response to tensile stress, in the mouse cranial suture wherein osteoblasts differentiate from MSCs. We found that the gene expression levels of several components involved in bone morphogenetic protein, Wnt, and epithelial growth factor signalings were elevated with tensile stress. Moreover gene expression of some extracellular matrices (ECMs), such as cysteine rich protein 61 (Cyr61)/CCN1 and galectin-9, were upregulated. These ECMs have the ability to modulate the activities of cytokines and are known as matricellular proteins. Cyr61/CCN1 expression was prominently increased in the fibroblastic cells and preosteoblasts in the suture. Thus, for the first time we demonstrated the mechanical stimulation of Cyr61/CCN1 expression in osteogenic cells in an ex vivo system. These results suggest the importance of matricellular proteins along with the cytokine-mediated signaling for the mechanical regulation of MSC proliferation and differentiation into osteoblastic cell lineage in vivo.

      DOI: 10.2220/biomedres.37.117

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      その他リンク: http://search.jamas.or.jp/link/ui/2016362875

    • Protective effect of blue-light shield eyewear for adults against light pollution from self-luminous devices used at night. 査読有り

      Masahiko Ayaki, Atsuhiko Hattori, Yusuke Maruyama, Masaki Nakano, Michitaka Yoshimura, Momoko Kitazawa, Kazuno Negishi, Kazuo Tsubota

      Chronobiol. Int.33 ( 1 ) 134 - 139   2016年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      We investigated sleep quality and melatonin in 12 adults who wore blue-light shield or control eyewear 2 hours before sleep while using a self-luminous portable device, and assessed visual quality for the two eyewear types. Overnight melatonin secretion was significantly higher after using the blue-light shield (P < 0.05) than with the control eyewear. Sleep efficacy and sleep latency were significantly superior for wearers of the blue-light shield (P < 0.05 for both), and this group reported greater sleepiness during portable device use compared to those using the control eyewear. Participants rated the blue-light shield as providing acceptable visual quality.

      DOI: 10.3109/07420528.2015.1119158

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    • Monohydroxylated polycyclic aromatic hydrocarbons influence spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus) 査読有り

      Nobuo Suzuki, Shouzo Ogiso, Koji Yachiguchi, Kimi Kawabe, Fumiya Maldno, Akira Toriba, Masato Kiyomoto, Toshio Sekiguchi, Yoshiaki Tabuchi, Takashi Kondo, Kei-ichiro Kitamura, Chun-Sang Hong, Ajai K. Srivastav, Yuji Oshima, Atsuhiko Hattori, Kazuichi Hayakawa

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY171   55 - 60   2015年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs), which are metabolites of polycyclic aromatic hydrocarbons (PAHs), act on calcified tissue and suppress osteoblastic and osteoclastic activity in the scales of teleost fish. The compounds may possibly influence other calcified tissues. Thus, the present study noted the calcified spicules in sea urchins and examined the effect of both PAHs and OHPAHs on spicule formation during the embryogenesis of sea urchins. After fertilization, benz[a]anthracene (BaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) were added to seawater at concentrations of 10(-8) and 10(-7) M and kept at 18 degrees C. The influence of the compound was given at the time of the pluteus larva. At this stage, the length of the spicule was significantly suppressed by 4-OHBaA (10(-8) and 10(-7) M). BaA (10(-7) M) decreased the length of the spicule significantly, while the length did not change with BaA (10(-8) M).The expression of mRNAs (spicule matrix protein and transcription factors) in the 4-OHBaA (10(-7) M)-treated embryos was more strongly inhibited than were those in the BaA (10(-7) M)-treated embryos. This is the first study to demonstrate that OHPAHs suppress spicule formation in sea urchins. (C) 2015 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.cbpc.2015.02.004

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    • Melatonin Inhibits Embryonic Salivary Gland Branching Morphogenesis by Regulating Both Epithelial Cell Adhesion and Morphology 査読有り 国際誌

      Obana-Koshino Aya, Ono Hitomi, Miura Jiro, Sakai Manabu, Uchida Hitoshi, Nakamura Wataru, Nohara Kanji, Maruyama Yusuke, Hattori Atsuhiko, Sakai Takayoshi

      PLOS ONE10 ( 4 ) e0119960   2015年4月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1371/journal.pone.0119960

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    • Ultradian oscillation in expression of four nnelatonin receptor subtype genes in the pineal gland of the grass puffer, a sennilunar-synchronized spawner, under constant darkness 査読有り

      Ikegami Taro, Maruyama Yusuke, Doi Hiroyuki, Hattori Atsuhiko, Ando Hironori

      FRONTIERS IN NEUROSCIENCE9   2015年1月

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      記述言語:英語  

      DOI: 10.3389/fnins.2015.00009

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    • Diurnal and Circadian Oscillations in Expression of Kisspeptin, Kisspeptin Receptor and Gonadotrophin-Releasing Hormone 2 Genes in the Grass Puffer, A Semilunar-Synchronised Spawner 査読有り

      Ando H, Ogawa S, Shahjahan Md, Ikegami T, Doi H, Hattori A, Parhar I

      JOURNAL OF NEUROENDOCRINOLOGY26 ( 7 ) 459 - 467   2014年7月

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      記述言語:英語  

      DOI: 10.1111/jne.12165

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    • Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish 査読有り

      Koji Yachiguchi, Noriko Matsumoto, Yuki Haga, Motoharu Suzuki, Chisato Matsumura, Masahiro Tsurukawa, Toshihiro Okuno, Takeshi Nakano, Kimi Kawabe, Kei-ichiro Kitamura, Akira Toriba, Kazuichi Hayakawa, Vishwajit S. Chowdhury, Masato Endo, Atsuhiko Chiba, Toshio Sekiguchi, Masaki Nakano, Yoshiaki Tabuchi, Takashi Kondo, Shigehito Wada, Hiroyuki Mishima, Atsuhiko Hattori, Nobuo Suzuki

      ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH21 ( 10 ) 6365 - 6372   2014年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:SPRINGER HEIDELBERG  

      To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.

      DOI: 10.1007/s11356-012-1347-5

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    • Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone 査読有り

      Koji Yachiguchi, Toshio Sekiguchi, Masaki Nakano, Atsuhiko Hattori, Megumi Yamamoto, Kei-ichiro Kitamura, Masahiro Maeda, Yoshiaki Tabuchi, Takashi Kondo, Hiromitsu Kamauchi, Hajime Nakabayashi, Ajai K. Srivastav, Kazuichi Hayakawa, Tatsuya Sakamoto, Nobuo Suzuki

      ZOOLOGICAL SCIENCE31 ( 5 ) 330 - 337   2014年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10(-5) and 10(-4) M) and MeHg (10(-6) to 10(-4) M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10(-5) and 10(-4) M) and MeHg (10(-)6 to 10(-4) M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

      DOI: 10.2108/zs130265

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    • Increased Prostaglandin E-2 Has a Positive Correlation with Plasma Calcium during Goldfish Reproduction 査読有り

      Nobuo Suzuki, Yusuke Maruyama, Masaki Nakano, Atsuhiko Hattori, Masato Honda, Yohei Shimasaki, Toshio Sekiguchi, Hiroyuki Mishima, Shigehito Wada, Ajai K. Srivastav, Kazuichi Hayakawa, Yuji Oshima

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY59 ( 1 ) 97 - 101   2014年2月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS  

      We recently demonstrated that prostaglandin E, (PG(2)) increases osteoclastic activity and induces bone resorption in both in vitro and in vivo experiments using goldfish. In the fish reproductive period, the plasma calcium (Ca) level in female teleosts increases remarkably to make vitellogenin, which is a major component of egg protein and a Ca binding protein. In this period, however, there is no reported relationship between PGE(2) and Ca metabolism in fish. To clarify the Ca metabolism in fish reproduction, we examined plasma PGE(2) and Ca levels and measured tartrate resistant acid phosphatase (TRAP) activities as an indicator of osteoclastic activity in goldfish. Plasma POE, levels in the reproductive stage significantly increased as compared with those in non reproductive stages. Also, both plasma Ca and TRAP increased in the reproductive stage. Significant positive correlations were recognized between plasma Ca and the gonad somatic index (r=0.81, p&lt;0.001), plasma Ca and plasma PGE(2) levels (r=0.635, p&lt;0.05), and plasma Ca and plasma TRAP activities (r=0.584, p&lt;0.05) from the analysis using samples of both reproductive and non reproductive stages. Taking these data into consideration, we suggested that PGE(2) acts on osteoclasts and increases plasma Ca as a result of osteoclastic bone resorption, and we concluded that PGE(2), is an important hormone in Ca metabolism during fish reproduction.

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    • Zebrafish scales respond differently to in vitro dynamic and static acceleration: Analysis of interaction between osteoblasts and osteoclasts 査読有り

      Kitamura Kei-ichiro, Takahira Koh, Inari Masato, Satoh Yusuke, Hayakawa Kazuichi, Tabuchi Yoshiaki, Ogai Kazuhiro, Nishiuchi Takumi, Kondo Takashi, Mikuni-Takagaki Yuko, Chen Wenxi, Hattori Atsuhiko, Suzuki Nobuo

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY166 ( 1 ) 74 - 80   2013年9月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1016/j.cbpa.2013.04.023

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    • Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales 査読有り

      Sachiko Yano, Kei-ichiro Kitamura, Yusuke Satoh, Masaki Nakano, Atsuhiko Hattori, Toshio Sekiguchi, Mika Ikegame, Hiroshi Nakashima, Katsunori Omori, Kazuichi Hayakawa, Atsuhiko Chiba, Yuichi Sasayama, Sadakazu Ejiri, Yuko Mikuni-Takagaki, Hiroyuki Mishima, Hisayuki Funahashi, Tatsuya Sakamoto, Nobuo Suzuki

      ZOOLOGICAL SCIENCE30 ( 3 ) 217 - 223   2013年3月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-kappa B ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain.

      DOI: 10.2108/zsj.30.217

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    • 象牙質の成長線の周期と体内時計の情報伝達分子のメラトニンの分泌リズムの関連 査読有り

      三島弘幸, 井上昌子, 門田理佳, 服部淳彦, 鈴木信雄, 筧 光夫, 松本敬, 里村一人, 見明康雄

      日本再生歯科医学会誌11   27 - 39   2013年

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      記述言語:日本語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.11223/jard.11.27

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    • Molecular neuroendocrine basis of lunar-related spawning in grass puffer 査読有り

      Ando Hironori, Shahjahan Md, Hattori Atsuhiko

      GENERAL AND COMPARATIVE ENDOCRINOLOGY181   211 - 214   2013年1月

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      記述言語:英語  

      DOI: 10.1016/j.ygcen.2012.07.027

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    • プロスタグランジンE2はキンギョにおいて鱗の骨芽細胞活性化と破骨細胞活性化を増強させ、カルシウム代謝に関与する(Prostagiandln E2 Increases Both Osteoblastic and Osteoclastic Activity in the Scales and Participates in Calcium Metabolism in Goldfish)

      Omori Katsunori, Wada Shigehito, Maruyama Yusuke, Hattori Atsuhiko, Kitamura Kei-ichiro, Sato Yusuke, Nara Masayuki, Funahashi Hisayuki, Yachiguchi Koji, Hayakawa Kazuichi, Endo Masato, Kusakari Rei, Yano Sachiko, Srivastav Ajai K, Kusui Takashi, Ejiri Sadakazu, Chen Wenxi, Tabuchi Yoshiaki, Furusawa Yukihiro, Kondo Takashi, Sasayama Yuichi, Nishiuchi Takumi, Nakano Masaki, Sakamoto Tatsuya, Suzuki Nobuo

      Zoological Science29 ( 8 ) 499 - 504   2012年8月

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      記述言語:英語   出版者・発行元:(公社)日本動物学会  

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    • Expression of osteoblastic and osteoclastic genes during spontaneous regeneration and autotransplantation of goldfish scale: A new tool to study intramembranous bone regeneration 査読有り

      Thiparpa Aime Thamamongood, Ryo Furuya, Shunsuke Fukuba, Masahisa Nakamura, Nobuo Suzuki, Atsuhiko Hattori

      BONE50 ( 6 ) 1240 - 1249   2012年6月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      Complementary DNA of osteoblast-specific genes (dlx5, runx2a, runx2b, osterix, RANKL, type I collagen, ALP, and osteocalcin) was cloned from goldfish (Carassius auratus) scale. Messenger RNA expressions were analyzed during spontaneous scale regeneration. Dlx5 had an early peak of expression on day 7, whereas osterix was constantly expressed during days 7-21. Runx2, a major osteoblastic transcription factor in mammalian bone, did not show any significant expression. The expressions of two functional genes, type I collagen and ALP, continually increased after day 7, while that of osteocalcin increased on day 14. As for osteoclastic markers, in addition to the cloning of two functional genes, TRAP and cathepsin K, in our previous study, we here cloned the transcription factor NFATc1 to use as an early osteoclastic marker. Using these bone markers, we investigate the signal key that controls the onset of scale resorption and regeneration by performing intra-scale-pocket autotransplantation of five groups of modified scales, namely, 1) methanol-fixed scale, 2) proteinase K-treated cell-free scale, 3) polarity reversal (upside-down) scale, 4) U-shape trimmed scale, and 5) circular-hole perforated scale. In this autotransplantation, each ontogenic scale was pulled out, modified, and then re-inserted into the same scale pocket. At post-transplant, inside the pockets of all modified transplant groups, new regenerating scales formed, attaching to the ongoing resorbed transplants. Autotransplantation of methanol-fixed scale, proteinase K-treated cell-free scale, and polarity reversal (upside-down) scale triggered scale resorption and scale regeneration. These two processes of scale resorption and regeneration occurred in accordance with osteoclastic and osteoblastic marker gene expressions. These results were microscopically confirmed using TRAP and ALP staining. Regarding the autotransplantation of U-shape trimmed and circular-hole perforated scales, new scales regenerated and grew at the trimmed/perforated part of each transplant, while scale resorption occurred apparently only around the trimmed/perforated area In contrast, no scale resorption or regeneration was detected in sham transplantations. Our finding suggests that loss of correct cell-to-cell contact between the scale-pocket lining cells and the scale cortex cells is the key to switch on the onset of scale resorption and regeneration. Overall, the present study shows that goldfish scale regeneration shares similarities in gene expression with intramembranous bone regeneration. Improved understanding of goldfish scale regeneration will help elucidate the process of intramembranous bone regeneration and make goldfish scale a possible new tool to study bone regeneration. (C) 2012 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.bone.2012.03.021

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    • The connection between the periodicity of incremental lines in the tooth dentin and the regulation by melatonin 査読有り

      Mishima H, Hattori A, Suzuki N, Tabata M. J, Kakei M, Miake Y, Suzuki M

      BONE50   S102 - S102   2012年5月

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      記述言語:英語  

      DOI: 10.1016/j.bone.2012.02.309

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    • Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation 査読有り

      Nobuo Suzuki, Toshio Sekiguchi, Honoo Satake, Kanoko Kato, Yudai Nishiyama, Hideya Takahashi, Janine A. Danks, T. John Martin, Atsuhiko Hattori, Masaki Nakano, Makiko Kakikawa, Sotoshi Yamada, Maho Ogoshi, Susumu Hyodo, Yoko Yamaguchi, Vishwajit S. Chowdhury, Kazuichi Hayakawa, Hisayuki Funahashi, Tatsuya Sakamoto, Yuichi Sasayama

      GENE499 ( 2 ) 326 - 331   2012年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE BV  

      In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish. (C) 2012 Elsevier B.V. All rights reserved.

      DOI: 10.1016/j.gene.2012.03.042

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    • Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish 査読有り

      Nobuo Suzuki, Janine A. Danks, Yusuke Maruyama, Mika Ikegame, Yuichi Sasayama, Atsuhiko Hattori, Masahisa Nakamura, Makoto J. Tabata, Toshio Yamamoto, Ryo Furuya, Kiyofumi Saijoh, Hiroyuki Mishima, Ajai K. Srivastav, Yukihiro Furusawa, Takashi Kondo, Yoshiaki Tabuchi, Ichiro Takasaki, Vishwajit S. Chowdhury, Kazuichi Hayakawa, T. John Martin

      BONE48 ( 5 ) 1186 - 1193   2011年5月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/m1-10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/m1-10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-kappa B ligand in osteoblasts and the receptor activator NF-kappa B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish. (C) 2011 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.bone.2011.02.004

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    • Effects of Inorganic Mmercury on Osteoclasts and Osteoblasts of the Goldfish Scales In Vitro 査読有り

      Nobuo Suzuki, Koji Yachiguchi, Kazuichi Hayakawa, Katsunori Omori, Koji Takada, Makoto J. Tabata, Kei-Ichiro Kitamura, Masato Endo, Shigehito Wada, Ajai K. Srivastav, Vishwajit S. Chowdhury, Yuji Oshima, Atsuhiko Hattori

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY56 ( 1 ) 47 - 51   2011年2月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS  

      The fish scales are the major source of internal calcium requirement due to having a higher internal calcium reservoir than the body skeleton during the periods of drastic calcium demand, such as sexual maturation. Therefore, we developed original in vitro assay system using goldfish scales that contain osteoclasts and osteoblasts, and examined the direct effect of inorganic mercury (HgCl2) on osteoclasts and osteoblasts. In this assay system, we measured the activities of tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as respective indicators of each activity in osteoclasts and osteoblasts. TRAP activity in the scales significantly decreased by the treatment of HgCl2 (10(-6)to 10(-3)M) during 6 hrs of incubation. In addition, mRNA expressions of osteoclastic markers: TRAP and cathepsin K significantly decreased compared with control. In our knowledge, this is the first report of a direct effect of inorganic mercury on osteoclasts. On the other hand, ALP activity decreased after exposures of HgCl2, at a concentration of 10(-6), 10(-5) or 10(-4)M for 36 and 64 hrs, although its activity did not change after 6 and 18 hrs. The mRNA expression of metallothionein (MT) which is a metal binding protein that protects the organism from heavy metal, significantly increased by HgCl2 (10(-4)M) although insulin like growth factor-I (osteoblastic marker) was less than those of control scales by treatment with HgCl2 (10(-4)M). These results suggests that osteoblasts may synthesize MT and protect from mercury until 18 hrs incubation. Thus, the scale in vitro assay system would be a useful means for analysis of heavy metal on bone metabolism.

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    • Synchronised expressions of LPXRFamide peptide and its receptor genes: seasonal, diurnal and circadian changes during spawning period in grass puffer.

      Shahjahan M, Ikegami T, Osugi T, Ukena K, Doi H, Hattori A, Tsutsui K, Ando H

      J Neuroendocrinol23 ( 1 ) 39 - 51   2011年1月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1111/j.1365-2826.2010.02081.x

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    • Osteoblast activity in the goldfish scale responds sensitively to mechanical stress 査読有り

      Kei-ichiro Kitamura, Nobuo Suzuki, Yusuke Sato, Tetsu Nemoto, Mika Ikegame, Nobuaki Shimizu, Takashi Kondo, Yukihiro Furusawa, Shigehito Wada, Atsuhiko Hattori

      COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY156 ( 3 ) 357 - 363   2010年7月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ELSEVIER SCIENCE INC  

      The adaptive response of bone to mechanical loading in teleosts is not well understood. We recently developed a new assay system using teleost scales, which consists of osteoblasts, osteoclasts, and bone matrix protein. In this system, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) were used as markers of osteoblasts and osteoclasts, respectively. Using this assay system, we examined the effects of mechanical loading on ALP and TRAP activity in goldfish scales. ALP activity in the scales was significantly elevated (p&lt;0.01) by ultrasound stimuli (1 MHz, 50% duty factor, 0.5 Hz pulse repetition frequency, 60 mW/cm(2) [I(SATA)] and 6 min) after both 18 h and 24 h of incubation while TRAP activity remained unchanged. In addition, mRNA expression of both insulin-like growth factor-I (IGF-I) and estrogen receptors (ER) increased significantly, as did ALP activity. After the goldfish had been swimming for 3 days (speed: 2 body lengths/second, duration: 3 h/day), the scales&apos; ALP activity increased significantly (p&lt;0.01) but TRAP activity did not change. These in vitro and in vivo results strongly suggest that osteoblasts in the goldfish scale respond sensitively to mechanical stress and may be important in promoting bone formation. (C) 2010 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.cbpa.2010.03.002

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    • Melatonin Stimulates the Release of Gonadotropin-Inhibitory Hormone by the Avian Hypothalamus

      Chowdhury, Vishwajit S, Yamamoto, Kazutoshi, Ubuka, Takayoshi, Bentley, George E, Hattori, Atsuhiko, Tsutsui, Kazuyoshi

      ENDOCRINOLOGY151 ( 1 ) 271 - 280   2010年

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    • Synchronized diurnal and circadian expressions of four subtypes of melatonin receptor genes in the diencephalon of a puffer fish with lunar-related spawning cycles.

      Ikegami T, Motohashi E, Doi H, Hattori A, Ando H

      Neurosci Lett462 ( 1 ) 58 - 63   2009年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1016/j.neulet.2009.06.076

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    • Effect of low-intensity pulsed ultrasound on osteoblasts and osteoclasts of the zebrafish scales 査読有り

      Suzuki N, Furusawa Y, Takasaki I, Tabuchi Y, Kitamura K, Wada S, Hori T, Kondo T, Nemoto T, Shimizu N, Hattori A

      BONE44   S157 - S157   2009年5月

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      記述言語:英語  

      DOI: 10.1016/j.bone.2009.01.347

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    • Monohydroxylated polycyclic aromatic hydrocarbons inhibit both osteoclastic and osteoblastic activities in teleost scales 査読有り

      Nobuo Suzuki, Kazuichi Hayakawa, Takayuki Kameda, Akira Triba, Ning Tang, Makoto J. Tabata, Koji Takada, Shigehito Wada, Katsunori Omori, Ajai K. Srivastav, Hiroyuki Mishima, Atsuhiko Hattori

      LIFE SCIENCES84 ( 13-14 ) 482 - 488   2009年3月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

      Aims: We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs) bound to a human estrogen receptor (ER) by a yeast two-hybrid assay, but polycyclic aromatic hydrocarbons did not have a binding activity. Therefore, the direct effect of 3-hydroxybenz[a]anthracene (3-OHBaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) on osteoclasts and osteoblasts in teleosts was examined. As a negative control, 1 -hydroxypyrene (1-OHPy), which has no binding activity to human ER, was used.
      Main methods: The effect of CHPAHs on osteoclasts and osteoblasts was examined by an assay system using teleost scale as each marker: tartrate-resistant acid phosphatase for osteoclasts and alkaline phosphatase for osteoblasts. Changes in cathepsin K (an osteoclastic marker) and insulin-like growth factor-1 (IGF-1) (an osteoblastic marker) mRNA expressions in 4-OHBaA-treated goldfish scales were examined by using a reverse transcription-polymerase chain reaction.
      Key findings: In both goldfish (a freshwater teleost) and wrasse (a marine teleost), the osteoclastic activity in the scales was significantly suppressed by 3-OHBaA and 4-OHBaA, although 1-OHPy did not affect the osteoclastic activity. In reference to osteoblasts, the osteoblastic activity decreased with both 3-CHBaA and 4-CHBaA and did not change with the 1-OHPy treatment. However, 17 beta-estradiol (E(2)) significantly increased both the osteoclastic and osteoblastic activities in the scales of both goldfish and wrasse. The mRNA expressions of both cathepsin K and IGF-1 decreased in the 4-OHBaA-treated scales but increased in the E(2)-treated scales.
      Significance: The current data are the first to demonstrate that 3-OHBaA and 4-OHBaA inhibited both osteoclasts and osteoblasts and disrupted the bone metabolism in teleosts. (C) 2009 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.lfs.2009.01.008

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    • Diurnal expressions of four subtypes of melatonin receptor genes in the optic tectum and retina of goldfish

      Taro Ikegami, Kyoichi Azuma, Masahisa Nakamura, Nobuo Suzuki, Atsuhiko Hattori, Hironori Ando

      Comparative Biochemistry and Physiology152   219 - 224   2009年

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      記述言語:英語  

      DOI: 10.1016/j.cbpa.2008.09.030

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    • Consumption of vegetables alters morning urinary 6-sulfatoxymelatonin concentration.

      Oba S, Nakamura K, Sahashi Y, Hattori A, Nagata C

      J Pineal Res45 ( 1 ) 17 - 23   2008年8月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1111/j.1600-079X.2007.00549.x

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    • Novel bromomelatonin derivatives suppress osteoclastic activity and increase osteoblastic activity: implications for the treatment of bone diseases 査読有り

      Nobuo Suzuki, Masanori Somei, Kei-Ichiro Kitamura, Russel J. Reiter, Atsuhiko Hattori

      JOURNAL OF PINEAL RESEARCH44 ( 3 ) 326 - 334   2008年4月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY-BLACKWELL  

      The teleost scale is a calcified tissue that contains osteoclasts, osteoblasts, and bone matrix, all of which are similar to those found in mammalian membrane bone. Using the goldfish scale, we recently developed a new in vitro assay system and previously demonstrated that melatonin suppressed both osteoclastic and osteoblastic activities in this assay system. In mammals, 2-bromomelatonin possesses a higher affinity for the melatonin receptor than does melatonin. Using a newly developed synthetic method, we synthesized 2-bromomelatonin, 2,4,6-tribromomelatonin and novel bromomelatonin derivatives (1-allyl-2,4,6-tribromomelatonin, 1-propargyl-2,4,6-tribromomelatonin, 1-benzyl-2,4,6-tribromomelatonin, and 2,4,6,7-tetrabromomelatonin) and then examined the effects of these chemicals on osteoclasts and osteoblasts. All bromomelatonin derivatives, as well as melatonin, had an inhibitory action on osteoclasts. In particular, 1-benzyl-2,4,6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. At an in vitro concentration of 10(-10) M, benzyl-tribromomelatonin still suppressed osteoclastic activity after 6 hr of incubation. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action, although melatonin inhibited osteoblastic activity. In addition, estrogen receptor mRNA expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(-7) M)-treated scales.Taken together the present,. results strongly suggest that these novel melatonin derivatives have significant potential for use as beneficial drug for bone diseases such as osteoporosis.

      DOI: 10.1111/j.1600-079X.2007.00533.x

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    • Scale osteoblasts and osteoclasts sensitively respond to low-gravity loading by centrifuge.

      SUZUKI Nobuo, OMORI Katsunori, NAKAMURA Masahisa, TABATA Makoto J, IKEGAME Mika, IJIRI Kenichi, KITAMURA Kei‐ichiro, NEMOTO Tetsu, SHIMIZU Nobuaki, KONDO Takashi, MATSUDA Kouhei, ANDO Hironori, KASAHARA Haruo, NAGASE Mutsumu, NARA Masayuki, HATTORI Atsuhiko

      Biol Sci Space22 ( 1 ) 3 - 7   2008年

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    • Prolactin inhibits osteoclastic activity in the goldfish scale: A novel direct action of prolactin in teleosts. 査読有り

      TAKAHASHI Hideya, SUZUKI Nobuo, TAKAGI Chiyo, IKEGAME Mika, YAMAMOTO Toshio, TAKAHASHI Akiyoshi, MORIYAMA Shunsuke, HATTORI Atsuhiko, SAKAMOTO Tatsuya

      ZOOLOGICAL SCIENCE25 ( 7 ) 739 - 745   2008年

    • Diurnal change of thyroid-stimulating hormone mRNA expression in the rat pars tuberalis 査読有り

      Aizawa S, Hoshino S, Sakata I, Adachi A, Yashima S, Hattori A, Sakai T

      JOURNAL OF NEUROENDOCRINOLOGY19 ( 11 ) 839 - 846   2007年11月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      DOI: 10.1111/j.1365-2826.2007.01603.x

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    • Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales 査読有り

      KyoIchi Azuma, Masaki Kobayashi, Masahisa Nakamura, Nobuo Suzuki, Sayaka Yashima, Shawichi Iwamuro, Mika Ikegame, Toshio Yamamoto, Atsuhiko Hattori

      BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS362 ( 3 ) 594 - 600   2007年10月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

      Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale. (c) 2007 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.bbrc.2007.08.010

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    • 新規1-ヒドロキシインドール誘導体の抗菌作用

      鈴木 信雄, 服部 淳彦, 唐原 一郎, 神阪 盛一郎, 染井 正徳

      日本薬学会年会要旨集127年会 ( 4 ) 128 - 128   2007年3月

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      記述言語:日本語   出版者・発行元:(公社)日本薬学会  

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    • S3-8 Effects of low-intensity ultrasound on bone metabolism in goldfish scale(Symposium (3) Biomedical Application)

      Kitamura Kei-ichiro, Suzuki Nobuo, Nemoto Tetsu, Shimizu Nobuaki, Tabata Makoto J., Wada Shigehito, Omori Katsunori, Nakamura Masahisa, Kondo Takashi, Hattori Atsuhiko

      ソノケミストリー討論会講演論文集2007   54 - 54   2007年

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      記述言語:英語   出版者・発行元:日本ソノケミストリー学会  

      DOI: 10.20577/pamjss.2007.suppl.0_54

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    • Effect of vibration on osteoblastic and osteoclastic activities: Analysis of bone metabolism using goldfish scale as a model for bone 査読有り

      Suzuki N, Kitamura K, Nemoto T, Shimizu N, Wada S, Kondo T, Tabata M. J, Sodeyama F, Ijiri K, Hattori A

      ADVANCES IN SPACE RESEARCH40 ( 11 ) 1711 - 1721   2007年

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    • The chemistry of indoles - part 128 - The ideal synthetic method aimed at the leads for an alpha(2)-blocker, an inhibitor of blood platelet aggregation, and an anti-osteoporosis agent 査読有り

      Masanori Somei, Takako Iwaki, Fumio Yamada, Yoshio Tanaka, Koki Shigenobu, Katsuo Koike, Nobuo Suzuki, Atsuhiko Hattori

      HETEROCYCLES68 ( 8 ) 1565 - 1569   2006年8月

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      記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

      According to the definition of the ideal synthetic method, an example aimed at the leads for an alpha(2)-blocker, an inhibitor of platelet aggregation, and an anti-osteoporosis agent is established starting from tryptamine. The originality rate, the intellectual property, and the application potential factors of the method are 71, 54, and 100, respectively. The method employs only conventional reagents and reaction conditions without using any protecting groups.

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    • 線維芽細胞成長因子-2の骨芽細胞活性化作用に対する表皮ブドウ球菌膜蛋白質による抑制

      倉持 大輔, 松下 和彦, 加藤 晴康, 河野 照茂, 五十嵐 潤子[右高], 平田 和明, 鈴木 信雄, 服部 淳彦, 別府 諸兄

      聖マリアンナ医科大学雑誌34 ( 4 ) 395 - 404   2006年

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      記述言語:日本語   出版者・発行元:聖マリアンナ医科大学医学会  

      骨感染症における骨形成能の低下や病的骨吸収は、細菌由来の因子や炎症性サイトカインなどの生体側の因子が作用して生じるとされているが、そのメカニズムは複雑で不明な点が多い。開放骨折部には皮膚の常在細菌である表皮ブドウ球菌が付着することがある。しかし、表皮ブドウ球菌が骨形成過程に及ぼす影響を検討した報告はみられない。そこで、本研究では、骨器官培養モデルとしてキンギョの再生ウロコを用い、皮膚の常在細菌である表皮ブドウ球菌由来の膜タンパク質が、骨折における骨形成過程に重要である線維芽細胞成長因子-2(FGF-2)に対して及ぼす影響を調べることを目的とした。再生ウロコの器官培養系を用い、FGF-2(1.0×10-7M)単独投与群と、FGF-2(1.0×10-7M)およびリチウムクロライドで抽出した表皮ブドウ球菌構成膜タンパク質の同時投与群を作製した。そして、骨芽細胞マーカーとしてアルカリフォスファターゼ(ALP)活性を、破骨細胞のマーカーとして酒石酸抵抗性酸フォスファターゼ(TRAP)活性を測定した。ALP活性は、FGF-2単独投与群では、コントロール群に対して有意に高値(p<0.05)を示した。FGF-2と表皮ブドウ球菌膜タンパク質の同時投与群では、FGF-2単独投与群に比べ、ALP活性は有意に低値を示した。一方、TRAP活性においては、FGF-2単独投与群とFGF-2と表皮ブドウ球菌膜タンパク質の同時投与群の間で、有意な差は認めなかった。さらにFGF抗体を用いた免疫染色により、再生ウロコにFGF-2の発現を認めた。以上の結果より、キンギョのウロコには、哺乳類と同等なFGF-2への反応性を有する骨芽細胞、および破骨細胞が存在することが明らかとなった。また、皮膚の常在菌である表皮ブドウ球菌を構成する膜タンパク質が、仮骨形成初期に発現するFGF-2の作用を明らかに抑制することが示された。すなわち、開放骨折において感染が持続していなくても、骨癒合が遷延化する可能性が示唆された。(著者抄録)

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      その他リンク: https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2006&ichushi_jid=J00746&link_issn=&doc_id=20061205140018&doc_link_id=%2Feq5maria%2F2006%2F003404%2F018%2F0395-0404%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Feq5maria%2F2006%2F003404%2F018%2F0395-0404%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

    • A2 超音波刺激による骨形成促進作用 : 魚のウロコのアッセイ系を用いた骨芽及び破骨細胞の解析

      鈴木 信雄, 北村 敬一郎, 根本 鉄, 清水 宣明, 和田 重人, 近藤 隆, 井尻 憲一, 田畑 純, 新実 信夫, 服部 淳彦

      ソノケミストリー討論会講演論文集15   3 - 4   2006年

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      記述言語:日本語   出版者・発行元:日本ソノケミストリー学会  

      We recently developed a new in vitro assay system for the evaluation of bone metabolism using goldfish scale. This system can simultaneously detect the activities of both scale osteoblasts (formation cells) and osteoclasts (resorption cells) with alkaline phosphatase and tartrate-resistant acid phosphatase as markers. Using this scale in vitro assay system, in the present study, we analyzed the effect of ultrasound (US) stimulation on osteoblastic and osteoclastic activities. The osteoblastic activity significantly increased by pulsed low-density US (1MHz, 60mW/cm^2 I_<SATA>, 50% duty factor at 0.5Hz, 180 pulses) in 18 hrs of incubation at 15℃ after US treatment but not in shorter incubation periods, while the osteoclastic activity did not change in the same incubation period. To examine the mechanism of US in osteoblasts, the estrogen receptor (ER) and insulin-like growth factor-I (IGF-I) mRNA expressions in the cultured scales were analyzed by RT-PCR. ER mRNA expression was found to be higher in the US-treated scales than in the control scales in 18 hrs of incubation at 15℃ after treatment, although ER mRNA expression did not change in 3 hrs of incubation. On the other hand, IGF-I mRNA expression increased in 3 hrs of incubation at 15℃ after US treatment. Therefore, IGF-I mRNA expression was more rapid to respond to US than ER mRNA expression, and IGF-I may have an important function in the activation of osteoblasts by US treatment. We found that both osteoblastic and osteoclastic activities increased in the remaining ontogenic scales of the left side at 3 days after the removal of all scales in the right side. Using these scales, we examined the effects of US stimulation on osteoblasts and osteoclasts under the same conditions described above. In 18 hrs of incubation at 15℃ after US treatment, the osteoblastic activity increased by US treatment in the ontogenic scale as well as the normal scale. Furthermore, we indicated that the osteoclastic activity in the ontogenic scale decreased by US treatment. The conditions of osteoblasts and osteoclasts in the ontogenic scale are similar to those in the human osteoporosis, indicating that US stimulation may contribute to the cure of osteoporosis.

      DOI: 10.20577/pamjss.15.0_3

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    • Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts. 査読有り

      SUZUKI Nobuo, TABATA Makoto J, KAMBEGAWA Akira, SRIVASTAV Ajai K, SHIMADA Atsuko, TAKEDA Hiroyuki, KOBAYASHI Masaki, WADA Shigehito, KATSUMATA Toshiyuki, HATTORI Atsuhiko

      LIFE SCIENCES78 ( 21 ) 2533 - 2541   2006年

    • Do goldfish scleroblasts correspond mammalian cells in bone? 査読有り

      Saijoh K, Suzuki N, Katsuyama H, Li LH, Higashi T, Tanii H, Tamada-Nishimura F, Hattori A

      FASEB JOURNAL19 ( 4 ) A58 - A58   2005年3月

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      記述言語:英語  

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    • The effect of melatonin in the IL-8 production and cell damage in vitro (using human normal gastric nucosal cell which has been cultured for more than one year). 査読有り

      Kojima T, Satoh K, Kawakami S, Kimura K, Hattori A

      GASTROENTEROLOGY114 ( 4 ) A1155 - A1155   1998年4月

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      記述言語:英語  

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    • Hydroxyindole-O-methyltransferase activity assay using high-performance liquid chromatography with fluorometric detection: Determination of melatonin enzymatically formed from N-acetylserotonin and S-adenosyl-L-methionine 査読有り

      Itoh MT, Hattori A, Sumi Y

      JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES692 ( 1 ) 217 - 221   1997年4月

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      記述言語:英語  

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    • Regulation by guanine nucleotides and cations of melatonin binding sites in the goldfish brain 査読有り

      Masayuki Iigo, Ritsuko Ohtani-Kaneko, Masayuki Hara, Atsuhiko Hattori, Hidehito Takahashi, Mitsuo Tabata, Takuro Suzuki, Katsumi Aida

      NeuroSignals6 ( 1 ) 29 - 39   1997年

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      記述言語:英語   掲載種別:研究論文(学術雑誌)  

      Effects of nucleotides and cations on 2-[125I]iodomelatonin binding sites in the goldfish brain were examined. Nucleotides (10–6–10–3 M) dose-dependently inhibited the specific binding with the following order of potency: guanosine 5''-O-(3-thiotriphosphate) (GTPγS) &gt
      GTP = GDP &gt
      GMP = ATP &gt
      cyclic GMP. Cyclic AMP was ineffective. The treatment of membranes with GTPγS induced rapid dissociation of 2-[125I]iodomelatonin from membranes when added at the steady state, increased the Kd and decreased the Bmax values as revealed by saturation analysis, and increased the IC50 value of melatonin to inhibit the specific binding. The treatment decreased the specific binding to membrane preparations obtained from six brain regions as well. Inorganic salts (5–200 mM) dose-dependently inhibited the specific binding with the following order of potency: CaCl2 &gt
      MgCl2 &gt
      LiCl &gt
      NaCl &gt
      choline chloride &gt
      KCl, except for 5 mM MgCl2, which enhanced the specific binding. Saturation experiments demonstrated that 75 mM CaCl2,100 mM MgCl2 and 200 mM NaCl increased the Kd and decreased the Bmax while 5 mM MgCl2 increased the Bmax value. These results imply that G protein and physiological concentrations of cations are involved in the regulation of melatonin binding sites in the goldfish brain. © 1997 S. Karger AG, Basel.

      DOI: 10.1159/000109106

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    • Characterization, Guanosine 5'-O-(3-thiotriphosphate) modulation, daily variation, and localization of melatonin-binding sites in the catfish (Silurus a sotus) Brain (共著) 査読有り

      Iigo M, Hattori A, Ohtani-Kaneko R, Aida K

        108   45 - 55   1997年

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    • Melatonin and glucose metabolism 査読有り

      Kazuki Watanabe, Sayaka Katagiri, Atsuhiko Hattori

      Glycative Stress Research7 ( 1 ) 105 - 109   2020年4月

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      記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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    • 骨粗鬆症モデルラットにおける副甲状腺ホルモンとメラトニンの併用効果

      池亀 美華, 田中 みか子, 江尻 貞一, 服部 淳彦, 高尾 亮子, 内部 健太, 岡村 裕彦

      Journal of Oral Biosciences Supplement2018   206 - 206   2018年9月

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      記述言語:日本語   出版者・発行元:(一社)歯科基礎医学会  

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    • 軟骨におけるメラトニンとその受容体の日周的合成は軟骨の律動的遺伝子発現に影響を及ぼす(Circadian production of melatonin and its receptors in cartilage influences chondrocyte rhythmic gene expression)

      Fu Shanqi, 桑原 実穂, 内田 瑶子, 林 大智, 下村 侑司, 高垣 安紗美, 西田 崇, 丸山 雄介, 池亀 美華, 服部 淳彦, 服部 高子, 久保田 聡

      日本生化学会大会プログラム・講演要旨集91回   [1T14m - 07(1P   2018年9月

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      記述言語:英語   出版者・発行元:(公社)日本生化学会  

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    • メラトニン-睡眠とアンチエイジング 招待有り

      服部淳彦

      全薬Journal ( 284 ) 10 - 13   2018年4月

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      記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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    • Toxicities of polycyclic aromatic hydrocarbons in fish and marine invertebrates.

      Suzuki, N, Ikari, T, Sato, M, Toriba, A, Sekiguchi, T, Kitani, Y, Ogiso, S, Yachiguchi, K, Hattori, A, Oshima, Y, Hayakawa, K

      Polycyclic Aromatic Hydrocarbons: Environmental Behavior and Toxicity in East Asia   245 - 259   2018年1月

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      記述言語:英語   掲載種別:記事・総説・解説・論説等(その他)   出版者・発行元:Springer  

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    • 軟骨組織におけるメラトニン合成とその受容体発現は概日リズムを持ち、軟骨細胞の代謝に影響を及ぼす

      服部 高子, Fu Shanqi, 桑原 実穂, 内田 瑶子, 近藤 星, 林 大智, 下村 侑司, 高垣 安紗美, 西田 崇, 丸山 雄介, 池亀 美華, 服部 淳彦, 久保田 聡

      生命科学系学会合同年次大会2017年度   [2P - 1190]   2017年12月

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      記述言語:日本語   出版者・発行元:生命科学系学会合同年次大会運営事務局  

      J-GLOBAL

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    • キンギョ鱗の骨組織研究への応用 招待有り 査読有り

      池亀美香, 服部淳彦, 鈴木信雄

      The Bone31 ( 2 ) 3 - 8   2017年8月

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      記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

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    • 概日リズム同調因子メラトニンによる象牙質や象牙芽細胞の組織構造への影響

      三島弘幸, 田辺咲貴, 服部淳彦, 鈴木信雄, 筧光男, 松本敬, 池亀美華, 見明康雄, 松本由樹

      化石研究会会誌50 ( 2 )   2017年

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    • メラトニンとエイジング 招待有り 査読有り

      服部淳彦

      比較生理生化学34 ( 1 ) 2 - 11   2017年1月

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      記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)   出版者・発行元:日本比較生理生化学会  

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      その他リンク: http://search.jamas.or.jp/link/ui/2017246704

    • Fish scale is a suitable model for analyzing determinants of skeletal fragility in type 2 diabetes

      Nobuo Suzuki, Kei-ichiro Kitamura, Atsuhiko Hattori

      ENDOCRINE54 ( 3 ) 575 - 577   2016年12月

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      記述言語:英語   掲載種別:その他   出版者・発行元:SPRINGER  

      DOI: 10.1007/s12020-016-1153-9

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    • Effects of Low-Intensity Pulsed Ultrasound (LIPUS) on Osteoclasts and Osteoblasts: Analysis Using an Assay System With Fish Scale as a Model of Bone. 国際誌

      J Orthop Trauma30 ( 8 ) S4-S4 - S4   2016年

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    • メラトニンが象牙質の組織構造,象牙芽細胞や成長線周期に及ぼす影響

      三島弘幸, 尾崎真帆, 武内昇平, 武市和彦, 服部淳彦, 鈴木信雄, 筧光夫, 松本敬, 池亀美華, 池亀美華, 見明康雄

      日本再生歯科医学会誌13 ( 1 )   2015年

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    • メラトニンの骨代謝に対する作用と骨疾患の治療への応用 (特集 メラトニンとアンチエイジング)

      鈴木 信雄, 関 あずさ, 服部 淳彦

      Anti-aging medicine10 ( 5 ) 697 - 702   2014年10月

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      記述言語:日本語   出版者・発行元:メディカルレビュー社  

      CiNii Article

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      その他リンク: http://search.jamas.or.jp/link/ui/2015015043

    • メラトニンと宇宙と骨代謝

      服部 淳彦, 五十嵐-右高 潤子, 池亀 美華

      内分泌・糖尿病・代謝内科38 ( 2 ) 190 - 200   2014年2月

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      記述言語:日本語   出版者・発行元:科学評論社  

      CiNii Article

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      その他リンク: http://search.jamas.or.jp/link/ui/2014143533

    • メラトニン投与による象牙質の組成や組織構造の変化に関する分析学的及び組織学的研究

      三島弘幸, 門田理佳, 尾碕真帆, 服部淳彦, 鈴木信雄, 筧光男, 松本敬, 池亀美華, 見明康雄

      日本再生歯科医学会誌12 ( 1 ) 11 - 22   2014年

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      記述言語:日本語  

      DOI: 10.11223/jard.12.11

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    • キンギョを用いた糖尿病様モデルのインスリン産生能の解析

      北村敬一郎, 安藤忠, 桶作若菜, 遠藤雅人, 服部淳彦, 鈴木信雄

      日本神経内分泌学会学術集会プログラム・抄録集40th   2013年

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    • キンギョウロコのアパタイトの解析-赤外ラマン分光によるアプローチ

      奈良雅之, 服部淳彦, 大西晃宏, 赤塚陽子, 矢野幸子, 鈴木信雄, 松田准一

      バイオイメージング22 ( 2 )   2013年

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    • 魚類のウロコを用いた評価系の開発と骨代謝研究への応用

      鈴木 信雄, 舟橋 久幸, 耿 啓達, 柿川 真紀子, 山田 外史, 廣田 憲之, 北村 敬一郎, 清水 宣明, 早川 和一, 三島 弘幸, 岩坂 正和, 上野 照剛, 大森 克徳, 矢野 幸子, 池亀 美華, 田渕 圭章, 和田 重人, 近藤 隆, 服部 淳彦

      まぐね = Magnetics Japan7 ( 4 ) 174 - 178   2012年8月1日

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      記述言語:日本語   出版者・発行元:日本応用磁気学会  

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    • メラトニンの新規作用:骨に対する作用とその誘導体を用いた骨疾患治療薬の開発

      鈴木 信雄, 関 あずさ, 染井 正徳, 中村 正久, 矢野 幸子, 大森 克徳, 池亀 美華, 三島 弘幸, 早川 和一, 服部 淳彦

      比較内分泌学37 ( 143 ) 194 - 203   2011年

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      記述言語:日本語   出版者・発行元:Japan Society for Comparative Endocrinology  

      DOI: 10.5983/nl2008jsce.37.194

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    • 歯の象牙質における成長線の周期性と生物リズムとの関連 : メラトニンによる調節の可能性

      三島 弘幸, 北原 正大, 服部 淳彦, 鈴木 信雄, 田畑 純, 奈良 雅之, 筧 光男, 見明 康夫

      比較内分泌学 = Comparative endocrinology36 ( 137 ) 106 - 112   2010年5月31日

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      記述言語:日本語   出版者・発行元:日本比較内分泌学会  

      DOI: 10.5983/nl2008jsce.36.106

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      その他リンク: http://search.jamas.or.jp/link/ui/2010234144

    • Novel bromomelatonin derivatives as potentially effective drugs to treat bone diseases

      Nobuo Suzuki, Masanori Somei, Azusa Seki, Russel J. Reiter, Atsuhiko Hattori

      JOURNAL OF PINEAL RESEARCH45 ( 3 ) 229 - 234   2008年10月

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      記述言語:英語   掲載種別:書評論文,書評,文献紹介等   出版者・発行元:WILEY-BLACKWELL  

      Several reports indicate that melatonin is involved in the regulation of bone metabolism. To examine the direct effect of melatonin on osteoclasts and osteoblasts, we developed an in vitro assay using fish scales that contain osteoclasts, osteoblasts, and bone matrix, all of which are similar to those found in mammalian membrane bone. Using the assay, we demonstrated that melatonin suppressed osteoclastic and osteoblastic activities. These findings are in agreement with the reports from in vivo studies in mice and rats. In an attempt to develop molecules that increase bone mass, novel bromomelatonin derivatives were synthesized, and the effects of these chemicals on osteoclasts and osteoblasts using the scale assay were examined. As a result, novel bromomelatonin derivatives with the ability to possibly increase bone formation were identified. In scale osteoclasts, particularly, 1-benzyl-2,4,6-tribromomelatonin had a more potent activity than melatonin. In reference to osteoblasts, this agent (10(-9)-10(-6) M) significantly activated osteoblasts. The effect of 1-benzyl-2,4,6-tribromomelatonin on bone formation was confirmed in ovariectomized rats. Thus, the oral administration of 1-benzyl-2,4,6-tribromomelatonin augmented the total bone mineral density of the femoral metaphysis of ovariectomized rats. The stress-strain index of the diaphysis in 1-benzyl-2,4,6-tribromomelatonin-treated rats significantly increased in comparison with that in ovariectomized rats. In rats fed a low-calcium diet, the total bone mineral density of the femoral metaphysis significantly increased following the oral administration of 1-benzyl-2,4,6-tribromomelatonin. These studies identified a melatonin derivative that may have potential use in the treatment of bone diseases, such as osteoporosis.

      DOI: 10.1111/j.1600-079X.2008.00623.x

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    • バイブレーションによる加速度負荷に対する骨芽細胞及び破骨細胞の応答

      鈴木 信雄, 北村 敬一郎, 根本 鉄, 服部 淳彦

      年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University2007   109 - 110   2008年7月31日

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      記述言語:日本語   出版者・発行元:金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan  

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    • 新規ブロモメラトニン誘導体の破骨細胞及び骨芽細胞に対する影響

      鈴木 信雄, 染井 正徳, 北村 敬一郎, 服部 淳彦

      年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University2007   111 - 112   2008年7月31日

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      記述言語:日本語   出版者・発行元:金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan  

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    • ANALYSIS OF OSTEOBLAST SPECIFIC MESSENGER RNA EXPRESSION IN THE REGENERATING SCALE OF GOLDFISH, Carassius auratus

      FURUYA Ryo, KOBAYASHI Masaki, NAKAMURA Masahisa, SUZUKI Nobuo, HATTORI Atsuhiko

      Proceedings of the Japan Society for Comparative Endocrinology ( 22 ) 50 - 50   2007年10月12日

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      記述言語:英語  

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    • ESTABLISHMENT OF EXPERIMENTAL MODEL FOR BONE DISEASE USING THE SCALE OF GOLDFISH, Carassius auratus

      THAMAMONGOOD Thiparpa, TABATA Jun, NAKAMURA Masahisa, SUZUKI Nobuo, HATTORI Atsuhiko

      Proceedings of the Japan Society for Comparative Endocrinology ( 22 ) 51 - 51   2007年10月12日

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      記述言語:英語  

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    • 魚鱗:硬組織研究と再生研究のフロンティア (特集 硬組織の進化)

      田畑 純, 鈴木 信雄, 服部 淳彦

      細胞39 ( 2 ) 55 - 57   2007年2月

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      記述言語:日本語   出版者・発行元:ニュー・サイエンス社  

      CiNii Article

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      その他リンク: http://search.jamas.or.jp/link/ui/2007135425

    • 歯の成長線の周期性にメラトニンが関与する可能性

      三島弘幸, 鈴木信雄, 田畑純, 大野由香, 中石裕子, 野村加代, 服部淳彦

      解剖学雑誌82 ( 3 )   2007年

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    • Effect of FGF-2 on osteoblasts and osteoclasts in regenerating scales of the goldfish

      Daisuke Kuramochi, Haruyasu Kato, Moroe Beppu, Junko Migitaka, Kazuaki Hirata, Nobuo Suzuki, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE23 ( 12 ) 1210 - 1210   2006年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Effect of melatonin on the differentiation of osteoclasts in the goldfish scale

      Kyouichi Azuma, Ryo Sugiura, Nobuo Suzuki, Masahisa Nakamura, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE23 ( 12 ) 1210 - 1210   2006年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Molecular cloning and gene expression of the key enzymes of melatonin synthesis in the goldfish scale

      Shinnosuke Miki, Sayaka Yashima, Nobuo Suzuki, Masahisa Nakamura, Atsuhiko Hattori, Shawichi Iwamuro

      ZOOLOGICAL SCIENCE23 ( 12 ) 1209 - 1209   2006年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Bromomelatonin derivatives suppressed osteoclastic activity but increased osteoblastic activity

      Nobuo Suzuki, Masanori Somei, Kei-Ichirou Kitarnura, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE23 ( 12 ) 1209 - 1209   2006年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Analysis of syndecans in regenerating and allotransplanted scales of the goldfish

      Toshiyuki Katsumata, Mitsuyo Okazaki, Nobuo Suzuki, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE23 ( 12 ) 1210 - 1210   2006年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • メラトニン Up to Date--骨とメラトニン

      服部 淳彦, 鈴木 信雄, 染井 正徳

      アンチ・エイジング医学2 ( 1 ) 78 - 86   2006年2月

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      記述言語:日本語   出版者・発行元:メディカルレビュー社  

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      その他リンク: http://search.jamas.or.jp/link/ui/2006140136

    • Molecular cloning of cDNAs encoding markers in osteoclasts, trap and cathepsin K in goldfish

      Masaki Kobayashi, Kyouichi Azuma, Nobuo Suzuki, Atsuhiko Hattori, Masahisa Nakamura

      ZOOLOGICAL SCIENCE22 ( 12 ) 1499 - 1499   2005年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Prolactin inhibits osteoclastic activities in the scales of goldfish

      Nobuo Suzuki, Tatsuya Sakamoto, Mika Ikegame, Toshio Yamamoto, Akiyoshi Takahashi, Shunsuke Moriyama, Hiroshi Kawauchi, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE22 ( 12 ) 1498 - 1498   2005年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • cDNA cloning of specific genes involved in the differentiation of osteoclasts in allotransplanted scales of the goldfish

      Akihiro Nishimura, Sakae Kikuyama, Nobuo Suzuki, Masayuki Hara, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE22 ( 12 ) 1499 - 1499   2005年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Expression analysis of Timp-2 during osteoclast activation in the scales of goldfish

      Kyouichi Azuma, Atsuhiko Hattori, Nobuo Suzuki, Masahisa Nakamura

      ZOOLOGICAL SCIENCE22 ( 12 ) 1499 - 1499   2005年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Changes of melatonin concentrations in the scale of female goldfish during reproductive period

      Sayaka Yashima, Shawichi Iwamuro, Nobuo Suzuki, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE22 ( 12 ) 1498 - 1499   2005年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • EFFECTS OF MELATONIN ON OSTEOCLAST DIFFERENTIATION INDUCED BY AN INTRAMUSCULAR AUTOTRANSPLANTATION OF THE GOLDFISH SCALE

      SUGIURA Ryo, AZUMA Kyoichi, KOBAYASHI Masaki, NAKAMURA Masahisa, SUZUKI Nobuo, HATTORI Atsuhiko

      Proceedings of the Japan Society for Comparative Endocrinology ( 20 ) 30 - 30   2005年11月12日

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      記述言語:英語  

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    • POSSIBLE FUNCTION OF MELATONIN ON CALCIUM METABOLISM IN THE SCALE OF FEMALE GOLDFISH IN THE REPRODUCTIVE STAGE

      YASHIMA Sayaka, IWAMURO Shawichi, SUZUKI Nobuo, HATTORI Atsuhiko

      Proceedings of the Japan Society for Comparative Endocrinology ( 20 ) 31 - 31   2005年11月12日

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      記述言語:英語  

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    • ANALYSIS OF HEPARAN SULFATE PROTEOGLYCANS IN THE SCALE OF GOLDFISH

      KATSUMATA Toshiyuki, OKAZAKI Mitsuyo, SUZUKI Nobuo, HATTORI Atsuhiko

      Proceedings of the Japan Society for Comparative Endocrinology ( 20 ) 29 - 29   2005年11月12日

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      記述言語:英語  

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    • Physiological role of melatonin in the scale of teleost

      N Suzuki, S Yashima, S Iwamuro, A Hattori

      BONE36   S302 - S302   2005年6月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ELSEVIER SCIENCE INC  

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    • Osteoblastic activity and estrogenic response in the regenerating scale of goldfish, a good model of osteogenesis

      H Yoshikubo, N Suzuki, K Takemura, M Hoso, S Yashima, S Iwamuro, Y Takagi, MJ Tabata, A Hattori

      LIFE SCIENCES76 ( 23 ) 2699 - 2709   2005年4月

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      記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

      Osteogenesis in the teleost was morphologically observed using regenerating scales of goldfish. Histological observations indicated that osteoblasts around the regenerating scales on days 7 to 10 were greater in size and number than those at other stages. Therefore, further experiments were carried out to examine the activity of osteoblasts in the regenerating period. To quantify their osteoblastic activities, scales on the left side of the body were taken, and the regenerating scales were then used to measure the activities of alkaline phosphatase (ALP), a marker of osteoblasts, on days 7, 10, and 15. The ontogenic scales on the right side of the body were also collected and used to measure ALP activity on the same days. Osteoblasts at all stages of regenerating scales were more active than those in the remaining ontogenic scales. The regenerating scales on day 10 had the highest activity. Furthermore, we found that estrogen receptor (ER) mRNA was expressed in the regenerating scales because estrogen participates in osteoblastic growth and differentiation in mammals. Therefore, using a scale culture system reported previously, the estrogenic response was examined in the ontogenic and regenerating scales on day 10. The reactivity was much higher in regenerating scales, although estrogen treatment significantly activated the osteoblastic activities in both scales. We are the first to demonstrate that ER is expressed in regenerating scales and that estrogen participates in osteogenesis as it does in mammalian bone. Our findings strongly suggest that regenerating scales can be used as a model of osteogenesis in vertebrates. (c) 2005 Elsevier Inc. All rights reserved.

      DOI: 10.1016/j.lfs.2004.10.063

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    • Daily and circadian variations of the pineal and ocular melatonin contents and their contributions to the circulating melatonin in the Japanese newt, Cynops pyrrhogaster

      A Chiba, A Hattori, M Iigo

      ZOOLOGICAL SCIENCE22 ( 1 ) 65 - 70   2005年1月

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      記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      Daily and circadian variations of melatonin contents in the diencephalic region containing the pineal organ, the lateral eyes, and plasma were studied in a urodele amphibian, the Japanese newt (Cynops pyrrhogaster), to investigate the possible roles of melatonin in the circadian system. Melatonin levels in the pineal region and the lateral eyes exhibited daily variations with higher levels during the dark phase than during the light phase under a light-dark cycle of 12 h light and 12 h darkness (LD12:12). These rhythms persisted even under constant darkness but the phase of the rhythm was different from each other. Melatonin levels in the plasma also exhibited significant day-night changes with higher values at mid-dark than at mid-light under LID 12:12. The day-night changes in plasma melatonin levels were abolished in the pinealectomized (Px), ophthalmectomized (Ex), and Px+Ex newts but not in the sham-operated newts. These results indicate that in the Japanese newts, melatonin production in the pineal organ and the lateral eyes were regulated by both environmental light-dark cycles and endogenous circadian clocks, probably located in the pineal organ and the retina, respectively, and that both the pineal organ and the lateral eyes are required to maintain the daily variations of circulating melatonin levels.

      DOI: 10.2108/zsj.22.65

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    • 松果体摘出ニワトリにおける椎体内骨化過程の違い

      鳥居 良昭, 金子 律子, 平田 和明, 笹生 豊, 三浦 竹彦, 磯見 卓, 加藤 晴康, 系 重毅, 望月 亜希, 正木 大賀, 青木 治人, 服部 淳彦

      脊柱変形 : 日本側彎症研究会会誌 = Spinal deformity : the journal of Japanese Scoliosis Society19 ( 1 ) 78 - 82   2004年12月20日

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      記述言語:日本語  

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    • Effects of melatonin on osteoclasts in the scale induced by intramuscular autotransplantation in goldfish

      Ryo Sugiura, Tetuya Kobayasi, Takeo Matida, Nobuo Suzuki, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE21 ( 12 ) 1339 - 1339   2004年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Mechanism of osteogenesis in teleosts: Analysis by regenerating scale of goldfish

      Nobuo Suzuki, Hiroaki Yoshikubo, Keiju Takemura, Masahiro Hoso, Sayaka Yashima, Shawichi Iwamuro, Yasuaki Takai, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE21 ( 12 ) 1339 - 1339   2004年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Seasonal changes in the concentrations of melatonin and 5-methoxytryptophol in the scales of male and female goldfish

      Sayaka Yashimia, Shawichi Iwamuro, Nobuo Suzuki, Atsuhiko Hattori

      ZOOLOGICAL SCIENCE21 ( 12 ) 1339 - 1339   2004年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Presence of melatonin and expression of N-acetyltransferase mRNA in the scales of goldfish

      Atsuhiko Hattori, Sayaka Yashima, Shawichi Iwamuro, Nobuo Suzuki

      ZOOLOGICAL SCIENCE21 ( 12 ) 1339 - 1339   2004年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Both mercury and cadmium directly influence calcium homeostasis resulting from the suppression of scale bone cells: the scale is a good model for the evaluation of heavy metals in bone metabolism

      N Suzuki, M Yamamoto, K Watanabe, A Kambegawa, A Hattori

      JOURNAL OF BONE AND MINERAL METABOLISM22 ( 5 ) 439 - 446   2004年9月

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      記述言語:英語   出版者・発行元:SPRINGER TOKYO  

      To examine the effects of heavy metals such as cadmium and mercury on calcium homeostasis, plasma calcium and calcitonin were measured in goldfish. Cadmium induced hypocalcemia both at 4 and at 8 days. In methylmercury-treated goldfish, the plasma calcium level increased at 2 days and then decreased at 8 days. The plasma calcitonin level increased in correspondence with the increased plasma calcium by methylmercury treatment, although cadmium did not cause a significant change. To elucidate the mechanism in detail, fish scales, which have both osteoclasts and osteoblasts and are similar to mammalian membrane bone, were used in the present study. We measured tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activity as respective indicators of activity in both types of cells. TRAP activity in the scales decreased by treatment of cadmium and methylmercury at 6 h incubation. Particularly, cadmium (even at 10(-13) M) significantly suppressed TRAP activity, suggesting that this system is utilized as an acute biosensor for cadmium. ALP activity decreased after exposures of 64 and 96 h, although the activity did not change after 6, 18, and 36 h. In addition, mRNA expression of the estrogen receptor and insulin-like growth factor 1, which participate in osteoblastic growth and differentiation, was less than the control values by treatment with both metals. This study demonstrates that mercury directly acts on the bone cells and influences calcium homeostasis and indicates that, in a short-term exposure, mercury has a different action from that of cadmium and induces hypercalcemia.

      DOI: 10.1007/s00774-004-0505-3

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    • メラトニン分泌を促す生活療法

      服部淳彦

      Geriat. Med.42, 1121-1126   1121 - 1126   2004年

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    • Bisphenol A suppresses osteoclastic and osteoblastic activities in the cultured scales of goldfish

      N Suzuki, A Hattori

      LIFE SCIENCES73 ( 17 ) 2237 - 2247   2003年9月

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      記述言語:英語   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

      The direct effect of bisphenol A on osteoclasts and osteoblasts was examined using a culture system of goldfish scales. Tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were used as markers of osteoclasts and osteoblasts, respectively. Bisphenol A (10(-5) M) significantly suppressed both TRAP and ALP activities. These data were reproduced in an in vivo experiment. From an analysis of a reverse transcription-polymerase chain reaction in the in vitro-cultured scales, it was demonstrated that the insulin-like growth factor (IGF)-1 mRNA expression decreased by a bisphenol A treatment. On the other hand, 17beta-estradiol (E-2) stimulated both TRAP and ALP activities and did not change IGF-1 mRNA expression, suggesting that bisphenol A has a different effect from E-2 on bone metabolism. This study is the first to demonstrate that bisphenol A functions to suppress directly osteoblasts and osteoclasts among vertebrates, which strongly suggests that this scale in an in vitro assay system can be utilized for the evaluation of the effects of endocrine disrupters on bone cells. (C) 2003 Elsevier Inc. All rights reserved.

      DOI: 10.1016/S0024-3205(03)00603-9

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    • Bisphenol A influences the plasma calcium level and inhibits calcitonin secretion in goldfish

      N Suzuki, A Kambegawa, A Hattori

      ZOOLOGICAL SCIENCE20 ( 6 ) 745 - 748   2003年6月

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      記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      In teleosts, it is well known that plasma calcium levels increase as a result of treatment with estrogen for at least during 2 weeks and that calcitonin secretion is induced by estrogen. The present study examined the influence of bisphenol A on calcium homeostasis in goldfish and compared the above known estrogenic action. In goldfish kept in water containing bisphenol A (10(-6) M) the plasma calcium concentration increased significantly (P&lt;0.001) at 4 days but decreased significantly (P&lt;0.05) at 8 days. By the treatment of bisphenol A, calcitonin secretion was not induced until 4 days. At 8 days, however, plasma calcitonin, as well as calcium, decreased significantly (P&lt;0.05), although vitellogenin was detected in the plasma. Therefore, bisphenol A influences plasma calcium levels, but its action is different from that of estrogen, which indicates that bisphenol A affects the calcium homeostasis and might bring about abnormal conditions in teleosts.

      DOI: 10.2108/zsj.20.745

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    • EFFECTS OF BISPHENOL-A AND CADMIUM ON OSTEOCLASTS AND OSTEOBLASTS IN THE SCALES OF GOLDFISH(Endocrinology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

      Suzuki Nobuo, Hattori Atsuhiko

      Zoological science20 ( 12 ) 1600 - 1601   2003年

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      記述言語:英語   出版者・発行元:Zoological Society of Japan  

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      その他リンク: http://id.nii.ac.jp/1141/00038257/

    • Melatonin suppresses osteoclastic and osteoblastic activities in the scales of goldfish

      N Suzuki, A Hattori

      JOURNAL OF PINEAL RESEARCH33 ( 4 ) 253 - 258   2002年11月

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      記述言語:英語   出版者・発行元:BLACKWELL MUNKSGAARD  

      The effects of melatonin on osteoclastic and osteoblastic cells were examined using a culture system of the goldfish scale. Tartrate-resistant acid phosphatase (TRACP) and alkaline phosphatase (ALP) were used as markers of osteoclastic and osteoblastic cells, respectively. In Earle's minimum essential medium containing melatonin (10(-9) to 10(-5) M, activities of both enzymes in scales were significantly suppressed at 6 hr after incubation (TRACP: 10(- 8), 10(-6), 10(-5) M ALP: 10(-7) to 10(-5) M, but at 18 hr only ALP activity was significantly lowered (10(-8), 10(-7) M. Estradiol-17beta (E-2) enhanced both activities, which were significantly inhibited and brought down to the level of the controls when co-incubated with E-2 and melatonin (TRACP at 6 hr: 10(-9) to 10(-5) M ALP at 6 hr: 10(-7) M ALP at 18 hr: 10(-8) M). Moreover, using reverse-transcription polymerase chain reaction, the mRNA expression of the estrogen receptor (ER) and insulin-like growth factor (IGF)-1, which are related to osteoblastic growth and differentiation, was decreased in the melatonin-treated scales. These results suggest that melatonin acts directly on the scale osteoclastic and osteoblastic cells where it suppresses the ALP activity via down-regulation of ER and IGF-1 mRNAs expression. This is the first report on the function of melatonin in osteoclasts and on the suppressive effect of melatonin in osteoblasts among vertebrates.

      DOI: 10.1034/j.1600-079X.2002.02953.x

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    • EFFECTS OF MELATONIN ON OSTEOCLAST AND OSTEOBLAST CELLS IN THE SCALE OF GOLDFISH(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

      Suzuki Nobuo, Hattori Atsuhiko

      Zoological science19 ( 12 ) 1485 - 1485   2002年

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      記述言語:英語   出版者・発行元:Zoological Society of Japan  

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      その他リンク: http://id.nii.ac.jp/1141/00037298/

    • EFFECTS OF MELATONIN ON OSTEOCLASTIC AND OSTEOBLASTIC ACTIVITIES IN CULTURED FRONTAL BONES OF CHICKS(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :

      Sano Akimi, Tsubaki Tomohiro, Hanazono Makoto, Suzuki Nobuo, Hattori Atsuhiko

      Zoological science19 ( 12 ) 1485 - 1485   2002年

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      記述言語:英語   出版者・発行元:Zoological Society of Japan  

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      その他リンク: http://id.nii.ac.jp/1141/00037299/

    • 松果体除去ニワトリにおける実験的側湾症とIGF-1について

      脊柱変形17巻1-4   2002年

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    • メラトニンと睡眠

      Annual Review 神経20027-17   2002年

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    • EFFECTS OF ESTRADIOL ON DIFFERENTIATION OF REPRODUCTIVE ORGANS AND DEVELOPMENT OF THE SPINE IN CHICK EMBRYOS

      TSUBAKI Tomohiro, HATTORI Atsuhiko, NAKAYAMA Hitoshi, OKAZAKI Mitsuyo, WADA Masaru, IWAMURO Showichi, HANAZONO Makoto

      Proceedings of the Japan Society for Comparative Endocrinology16   30 - 30   2001年12月1日

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      記述言語:英語  

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    • Changes in melatonin concentrations during IFN therapy

      Y Uchimura, N Uchimura, R Kumashiro, A Hattori, M Sata

      JOURNAL OF HEPATOLOGY31 ( 6 ) 1131 - 1131   1999年12月

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      記述言語:英語   掲載種別:速報,短報,研究ノート等(学術雑誌)   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      DOI: 10.1016/S0168-8278(99)80329-8

      PubMed

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    • PLASMA CALCITONIN AND CA LEVELS IN CHICKS SUFFERED FROM BENDING OF VERTEBRAE BY PINEALECTOMY

      SUZUKI Daisuke, HATTORI Atsuhiko, KOMURO Sanae, SUZUKI Nobuo, SASAYAMA Yuichi, KAMBEGAWA Akira, ASAHINA Kiyosi

      Proceedings of the Japan Society for Comparative Endocrinology13   62 - 62   1998年7月

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      記述言語:英語  

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    • Effects of a low-protein diet on prolactin- and growth hormone-producing cells in the rat pituitary gland

      M Hara, DC Herbert, T Taniguchi, A Hattori, R Ohtani-Kaneko, M Iigo, Y Kato, K Hirata

      ANATOMICAL RECORD251 ( 1 ) 37 - 43   1998年5月

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      記述言語:英語   出版者・発行元:WILEY-LISS  

      Background. It is well known that an unbalanced diet induces various changes in the pituitary gland. However, little attention has been paid to the molecular aspects of this perturbation. We studied the influence of a low-protein diet (LPD) on the prolactin (PRL) and growth hormone (GH) cells in the rat pituitary gland using immunohistochemical staining and in situ hybridization.
      Materials: Rats aged 20 days were fed a diet containing 27% protein or one with 8% protein (LPD) for 30 days. Pituitary glands were obtained and subjected to either immunohistochemistry or in situ hybridization. Quantitative morphological analysis was then conducted to determine cell number and area as well as the percentage of cells stained by the respective antisera and/or cDNA probe in each experimental group.
      Results: The average sectional areas of both PRL-and GH-producing cells in the LPD group were smaller in size than those in the controls. The cell numbers per unit area (mm(2)) of PRL-positive cells and PRL mRNA-positive cells were 3,596.5 and 3,948.6, respectively, in the LPD group, and 3,179.6 and 4,888.5, respectively, in the controls. The numbers per unit area of GH-positive cells and GH mRNA-positive cells in the LPD group were similar (2,252.3 and 2,224.4), as compared to 2,161.3 and 1,684.2, respectively, in the well-fed rats. Whereas PRL-positive cells comprised about 27% of the total number of cells in both animal groups, those given the LPD contained a lower percentage (29%) of PRL mRNA-positive cells as compared to the controls (44%). On the other hand, GH mRNA-positive cells numbered about 15% of the total cell population both animal groups; however, the malnourished rats contained a lower percentage (16%) of GH-positive cells than did their well-fed counterparts (20%).
      Conclusions: Taken together, these results indicate that in the rat pituitary gland, administration of an LPD reduced the size of PRL- and GH-positive cells as well as differentially affecting a subpopulation of the PRL mRNA-positive cells and the GH-positive cells. (C) 1998 Wiley-Liss, Inc.

      DOI: 10.1002/(SICI)1097-0185(199805)251:1<37::AID-AR7>3.0.CO;2-B

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    • 実験的側湾症におけるIGF-1発現の免疫組織学的検討

      聖マリアンナ医大誌26巻417-423   1998年

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    • メラトニンのIL-8分泌に及ぼす影響

      実験潰瘍25   248 - 250   1998年

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    • ONTOGENESIS OF PROLACTIN GENE EXPRESSION IN RAT FETAL PITUITARY GLAND USED BY RT-PCR METHOD

      SATO Kenjiro, HATTORI Atsuhiko, WADA Masaru, IGARASHI Junko, HARA Masayuki, HIRATA Kazuaki, IMAI Kiyoshi

      Proceedings of the Japan Society for Comparative Endocrinology12   31 - 31   1997年7月

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      記述言語:英語  

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    • Ocular melatonin rhythms in the goldfish, Carassius auratus

      M Iigo, K Furukawa, A Hattori, R OhtaniKaneko, M Hara, T Suzuki, M Tabata, K Aida

      JOURNAL OF BIOLOGICAL RHYTHMS12 ( 2 ) 182 - 192   1997年4月

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      記述言語:英語   出版者・発行元:SAGE PUBLICATIONS INC  

      Ocular melatonin rhythms in the goldfish were studied and compared to those in the pineal organ and plasma. Under Light:dark (LD) of 12 h light:12 h dark, melatonin contents in the eye as well as the pineal organ and plasma exhibited clear day-night changes with higher levels at mid-dark than at mid-light. However, melatonin contents in the eye at mid-light and mid-dark were approximately 100 and 9 times greater than those in the pineal organ, respectively. Day-night changes of ocular melatonin persisted after pinealectomy, which abolished those in plasma melatonin under LD 12:12. Ocular melatonin contents in the pinealectomized fish at mid-light were significantly higher than those in the sham-operated control. Under constant darkness (DD), circadian melatonin rhythms were observed in the eye but damped on the 3rd day, whereas plasma melatonin rhythms generated by the pineal organ persisted for at least 3 days. Under constant light, ocular melatonin contents exhibited a significant fluctuation with a smaller amplitude than that under DD, whereas plasma melatonin remained at low levels. These results indicate the involvement of LD cycles, a circadian clock, and the pineal organ in the regulation of ocular melatonin rhythms in the goldfish.

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    • Hydroxyindole-O-methyltransferase activity assay using high-performance liquid chromatography with fluorometric detection: Determination of melatonin enzymatically formed from N-acetylserotonin and S-adenosyl-L-methionine

      MT Itoh, A Hattori, Y Sumi

      JOURNAL OF CHROMATOGRAPHY B692 ( 1 ) 217 - 221   1997年4月

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      記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

      A reliable, sensitive and rapid assay has been developed for determining the activity of hydroxyindole-O-methyltransferase (HIOMT; S-adenosyl-L-methionine:N-acetylserotonin-O-methyltransferase EC 2.1.1.4), which catalyzes the final step in the melatonin (N-acetyl-5-methoxytryptamine) biosynthetic pathway. This method is based on the separation and detection of melatonin formed enzymatically from N-acetylserotonin and S-adenosyI-L-methionine, by high-performance liquid chromatography with fluorometric detection. The detection limit far melatonin formed per sample was as low as 150 fmol, indicating that the sensitivity of this assay was comparable to that of a radioisotopic assay. The assay was applied to the determination of HIOMT activity in rat pineal gland. The HIOMT activity obtained in this study was comparable with, or slightly lower than those reported previously using radioisotopic assays.

      DOI: 10.1016/S0378-4347(96)00503-8

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    • メラトニン-不思議なホルモン?

      服部 淳彦

      化学と教育45 ( 5 ) 256 - 257   1997年

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      記述言語:日本語   出版者・発行元:公益社団法人 日本化学会  

      DOI: 10.20665/kakyoshi.45.5_256

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    • メラトニンと進化-多彩な機能とその評価

      脳と精神の医学8   319 - 324   1997年

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    • Regulation by guanine nucleotides and cations of melatonin binding sites in the goldfish brain

      M Iigo, R OhtaniKaneko, M Hara, A Hattori, H Takahashi, M Tabata, T Suzuki, K Aida

      BIOLOGICAL SIGNALS6 ( 1 ) 29 - 39   1997年1月

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      記述言語:英語   出版者・発行元:KARGER  

      Effects of nucleotides and cations on 2-[I-125]iodomelatonin binding sites in the goldfish brain were examined. Nucleotides (10(-6)-10(-3) M) dose-dependently inhibited the specific binding with the following order of potency: guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) &gt; GTP = GDP &gt; GMP = ATP &gt; cyclic GMP. Cyclic AMP was ineffective. The treatment of membranes with GTP gamma S induced rapid dissociation of 2-[I-125]iodomelatonin from membranes when added at the steady state, increased the K-d and decreased the B-max values as revealed by saturation analysis, and increased the IC50 value of melatonin to inhibit the specific binding. The treatment decreased the specific binding to membrane preparations obtained from six brain regions as well. Inorganic salts (5-200 mM) dose-dependently inhibited the specific binding with the following order of potency: CaCl2 &gt; MgCl2 &gt; LiCl &gt; NaCl &gt; choline chloride &gt; KCl, except for 5 mM MgCl2, which enhanced the specific binding. Saturation experiments demonstrated that 75 mM CaCl2, 100 mM MgCl2 and 200 mM NaCl increased the K-d and decreased the B-max while 5 mM MgCl2 increased the B-max value. These results imply that G protein and physiological concentrations of cations are involved in the regulation of melatonin binding sites in the goldfish brain.

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    • Retinal melatonin is not involved in corneal mitotic rhythms in the Japanese quail: Effects of formoguanamine hydrochloride and eye-lid suture

      T Oishi, Y Mohri, T Kaneko, M Sasaki, A Hattori, Y Obara, A Masuda

      JOURNAL OF PINEAL RESEARCH21 ( 3 ) 149 - 154   1996年10月

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      記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      Relation between retinal melatonin and corneal mitotic rhythms in the Japanese quail was investigated in experiments manipulating the ocular physiology by treatments with formoguanamine hydrochloride (FG) and eye-lid suture. In experiment 1, we investigated the effects of FG, which is known to induce photoreceptor degeneration, on retinal melatonin and corneal mitotic rhythms. FG-treatment completely abolished the retinal melatonin rhythms in both LD 12:12 and constant darkness (DD), but the corneal mitotic rhythm was maintained with high mitotic rate in darkness under a LD cycle and subjective night under DD. The result suggests that 1) the photoreceptor cells in the retina are the site for melatonin production and/or for the oscillator which drives the circadian rhythm in retinal melatonin, and 2) melatonin is not involved in generation of the corneal mitotic rhythm. In experiment 2, we investigated the effects of eye-lid suture, which is known to induce eye enlargement and bulgy cornea, on the retinal melatonin and corneal mitotic rhythms. Eye-lid suture abolished the corneal mitotic rhythm in both LD and DD, with a high mitotic rate being maintained throughout 24 hr. But retinal melatonin maintained its rhythm with high levels in darkness under a LD cycle and in subjective night under DD. The result suggests that 1) bulgy cornea in the sutured eye was induced by the increase in mitotic rate in the light period, and 2) disappearance of the corneal mitotic rhythm does not have a relation to retinal melatonin. These results suggest that retinal melatonin is not involved in generation of the corneal mitotic rhythm and that there are two circadian clock systems in the eye.

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    • Pit-1 gene expression in human pituitary adenomas using the reverse transcription polymerase chain reaction method

      S Yamada, M Takahashi, M Hara, A Hattori, T Sano, Y Ozawa, Y Shishiba, K Hirata, M Usui

      CLINICAL ENDOCRINOLOGY45 ( 3 ) 263 - 272   1996年9月

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      記述言語:英語   出版者・発行元:BLACKWELL SCIENCE LTD  

      OBJECTIVE Previous studies of Pit-1 expression in human pituitary tumours have produced conflicting results, We have studied expression of Pit-1 mRNA in human pituitary adenomas, as well as in normal human and rat pituitaries, and results were compared with clinical, histological, and immunohistochemical features, In addition, expression of GH, PRL, and TSH-beta mRNA was also studied and compared with Pit-1 gene expression.
      MATERIAL AND METHODS The adenomas consisted of 13 GH cell adenomas, 7 PRL cell adenomas, 3 TSH cell adenomas, 4 ACTH cell adenomas, and 10 clinically non-functioning adenomas. Expression of the Pit-1, its isoforms, and each hormone, was studied using the reverse transcription polymerase chain reaction.
      RESULTS Pit-1 mRNA was expressed not only in normal human and rat pituitaries, but also in all GH, PRL and TSH cell adenomas. There was no correlation between Pit-1 transcripts and biological behaviour or histological findings in these three types of adenoma, suggesting that Pit-1 is generally required for the determination of cell phenotype but is insufficient for the regulation of hormonal activity and tumour growth in these pituitary adenomas. In addition, Pit-1 was also expressed in some ACTH cell (2/4) and non-functioning adenomas (7/10), Although there were no GH, PRL or TSH-beta transcripts in Pit-1 mRNA-negative ACTH cell and non-functioning adenomas, PRL mRNA was detected in all Pit-1 mRNA-positive ACTH cell adenomas and GH, PRL and/or TSH-beta mRNA were found in four of seven Pit-1 mRNA-positive non-functioning adenomas, In contrast, Pit-1 mRNA was expressed without any GH, PRL, or TSH-beta transcripts in only three nonfunctioning adenomas, These data suggest that expression of Pit-1 mRNA in these two types of adenomas can be mainly attributed to the presence of GH, PRL and/or TSH-beta mRNA expressing cells and that true Pit-1 transcripts found in non-functioning adenomas may be rare event, Moreover, there were two cases which expressed Pit-1 alpha mRNA, but failed to show other Pit-1 isoform mRNA, There were, however, no clinical or histological differences between these two adenomas showing only Pit-1 alpha mRNA and the others expressing both Pit-1 alpha mRNA and other Pit-1 isoform mRNA.
      CONCLUSIONS Pit-1 mRNA was expressed not only in GH, PRL and TSH cell adenomas but also in other types of adenoma, However, it is suggested that expression of Pit-1 mRNA in most ACTH cell and non-functioning adenomas can be attributed to GH, PRL and/or TSH-beta mRNA expressing cells, Further studies are necessary to elucidate the role of Pit-1 transcripts in the three nonfunctioning adenomas without GH, PRL and/or TSH-beta mRNA expression.

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    • HORMONAL CHANGES IN PINEALECTOMIZED CHICKS WITH SPINAL DEFORMITY

      KATO Haruyasu, HATTORI Atsuhiko, WADA Masaru, AOKI Haruhito, HAMABE Masaki, SASAO Yutaka, WAKITA Masaaki

      Proceedings of the Japan Society for Comparative Endocrinology11   49 - 49   1996年8月

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      記述言語:英語  

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    • PRESENCE OF PINEAL HORMONE, MELATIONIN, IN THE PLANT

      HATTORI Atsuhiko, MIGITAKA Hiro, ITOH Masanori, IIGO Masayuki, OHTANI-KANEKO Ritsuko, HARA Masayuki, TAKAHASHI Hidehito, SUZUKI Takuro

      Plant and cell physiology37   150 - 150   1996年3月

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      記述言語:英語  

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    • 熱ショックストレスによる成長ホルモン遺伝子発現-培養ラット下垂体細胞を用いた解析

      聖マリアンナ医大誌24   6 - 15   1996年

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    • H.pylori 実験における正常胃由来培養細胞(HM-1)の有用性

      消化器科22   149 - 154   1996年

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    • MELATONIN AND ARYLALKYLAMINE N-ACETYLTRANSFERASE ACTIVITY IN THE SILKWORM, BOMBYX-MORI

      MT ITOH, A HATTORI, T NOMURA, Y SUMI, T SUZUKI

      MOLECULAR AND CELLULAR ENDOCRINOLOGY115 ( 1 ) 59 - 64   1995年11月

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      記述言語:英語   出版者・発行元:ELSEVIER SCI PUBL IRELAND LTD  

      Melatonin (N-acetyl-5-methoxytryptamine) was identified in the head and hemolymph of the silkworm, Bombyx mori, using reversed-phase high-performance liquid chromatography coupled with fluorometric detection and radioimmunoassay. In addition, evidence of arylalkylamine (serotonin) N-acetyltransferase (NAT) a key enzyme controlling the synthesis of melatonin in vertebrates, was found in the head of the silkworm. Melatonin levels in the head acid hemolymph and the NAT activity in the head were significantly higher during the dark period than during the light period of a 12-h light/12-h dark cycle. The day-night changes persisted in constant darkness but were suppressed by constant light. The results suggest that the synthesis and release of melatonin in the silkworm head occur as a circadian rhythm that is entrained by environmental light/dark cycles, as it is in the pineal gland of vertebrates. Melatonin in the silkworm head may function as a neurochemical mediator of photoperiodic control of developmental events such as molting, eclosion and diapause.

      DOI: 10.1016/0303-7207(95)03670-3

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    • Melatonin binding sites in the brain of the Japanese newt, Cynops pyrrhogaster

      IIGO Masayuki, CHIBA Atsuhiko, HATTORI Atsuhiko, HARA Masayuki, OHTANI-KANEKO Ritsuko, TAKAHASHI Hidehito, SUZUKI Takuro

      Proceedings of the Japan Society for Comparative Endocrinology10   56 - 56   1995年10月

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      記述言語:英語  

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    • EFFECTS OF TESTOSTERONE ON THE FUNCTION OF LH CELLS IN CASTRATED MALE RATS : ANALYSIS BY THE REVERSE HEMOLYTIC PLAQUE ASSAY

      HATTORI Atsuhiko, KAZAMA Yoshihiko, MIGITAKA Hiro, TAKAHASHI Hidehito, OHTANI-KANEKO Ritsuko, HARA Masayuki, IIGO Masayuki, SUZUKI Takuro

      Proceedings of the Japan Society for Comparative Endocrinology10   66 - 66   1995年10月

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      記述言語:英語  

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    • EFFECTS OF PINEALECTOMY AND CONSTANT LIGHT EXPOSURE ON DAY-NIGHT CHANGES OF MELATONIN BINDING-SITES IN THE GOLDFISH BRAIN

      M IIGO, K FURUKAWA, A HATTORI, M HARA, R OHTANIKANEKO, T SUZUKI, M TABATA, K AIDA

      NEUROSCIENCE LETTERS197 ( 1 ) 61 - 64   1995年9月

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      記述言語:英語   出版者・発行元:ELSEVIER SCI PUBL IRELAND LTD  

      Effects of pinealectomy and constant light exposure on day-night changes of melatonin binding sites in the goldfish brain were examined. The density and affinity of binding sites were higher at mid-day than at mid-night in sham-pinealectomized goldfish under light-dark cycles. The rhythms disappeared after pinealectomy, or constant light exposure both of which abolish plasma melatonin rhythms. The effects of pinealectomy and constant light exposure were not additive. These results indicate that diel changes of melatonin binding sites in the goldfish brain are regulated by endogenous melatonin of pineal origin.

      DOI: 10.1016/0304-3940(95)11903-A

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    • DAY-NIGHT CHANGES IN MELATONIN LEVELS IN DIFFERENT ORGANS OF THE CRICKET (GRYLLUS-BIMACULATUS)

      MT ITOH, A HATTORI, Y SUMI, T SUZUKI

      JOURNAL OF PINEAL RESEARCH18 ( 3 ) 165 - 169   1995年4月

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      記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      Day-night levels of melatonin (N-acetyl-5-methoxytryptamine) were de termined in different organs of adult female crickets (Gryllus bimaculatus) exposed to a 12/12 light/dark cycle, using reversed-phase high performance liquid chromatography coupled with fluorometric detection. Melatonin levels in the compound eye, brain, and palp were significantly higher during the dark period than during the light period, suggesting that a diurnal rhythm of melatonin levels exists in these organs of crickets, with a peak during the dark period. Conversely, melatonin levels were significantly higher during the light period than the dark period in the cercus, ovipositor, antenna, hind-leg and ovary. No significant day-night difference was found in the fore- and mid-legs, Malpighian tube, and digestive tube. Thus, these organs may have different melatonin-metabolizing systems compared to those found in the compound eye, brain, and palp. Differences in the phasing of the melatonin rhythm in various organs of the cricket suggest possible differences in melatonin function in these organs.

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    • IDENTIFICATION OF MELATONIN IN PLANTS AND ITS EFFECTS ON PLASMA MELATONIN LEVELS AND BINDING TO MELATONIN RECEPTORS IN VERTEBRATES

      A HATTORI, H MIGITAKA, M IIGO, M ITOH, K YAMAMOTO, R OHTANIKANEKO, M HARA, T SUZUKI, RJ REITER

      BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL35 ( 3 ) 627 - 634   1995年3月

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      記述言語:英語   出版者・発行元:ACADEMIC PRESS AUST  

      Twenty-four edible plants were investigated for the presence of melatonin, heretofore considered to be a molecule found only in the animal kingdom. The amount of melatonin in different plants varied greatly with highest melatonin being present in plants of the rice family. Melatonin was identified by radioimmunoassay and verified by high performance liquid chromatography with fluorescence detection. Feeding a diet containing plant products rich in melatonin to chicks increased radioimmunoassayable levels of melatonin in their blood. Likewise, melatonin extracted from plants inhibited binding of [I-125]iodomelatonin to rabbit brain. Thus, melatonin ingested in foodstuffs enters the blood and is capable of binding to melatonin binding sites in the brain of mammals.

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    • メラトニン投与によるサクラマス銀化制御の試み

      飯郷雅之, 須永史夫, 田畑満生, 生田和正, 北村章二, 服部淳彦, 原正幸, 高橋秀仁, 会田勝美

      日本水産学会大会講演要旨集1995   1995年

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    • 後天性免疫不全症候群に合併したサイトメガロウイルス食道潰瘍の1例

        46   91 - 93   1995年

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    • 暗期ラット血漿メラトニン濃度に及ぼす各種薬剤皮下投与の影響

      内田和秀

      聖マリアンナ医大誌23 ( 5 ) 1030 - 1034   1995年

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    • 去勢雄ラット下垂体gonadotrophsのLH放出に対するテストステロンの影響-reverse hemolytic plaque assayによる解析-

      聖マリアンナ医大誌23   2121 - 2131   1995年

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    • ウズラ胚の網膜形成過程におけるメラトニンの変化

      聖マリアンナ医大誌23   239 - 248   1995年

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    • ニワトリ松果体除去による脊柱側わん発生と脊椎骨におけるメラトニンレセプターの検索

      聖マリアンナ医大誌23   853 - 861   1995年

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    • Day-night changes in melatonin contents in the vitreous body and in vitro melatonin release from the retina of Japanese quail(Coturnix coturnix japonica).

      St. Marianna Med. J.23   833 - 842   1995年

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    • Perioperative plasma melatonin levels in patients with cardiac and esophageal surgery.

      St. Marianna Med. J.23   292 - 298   1995年

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    • MELATONIN INHIBITS LUTEINIZING-HORMONE-RELEASING HORMONE (LHRH) INDUCTION OF LH-RELEASE FROM FETAL-RAT PITUITARY-CELLS

      A HATTORI, DC HERBERT, MK VAUGHAN, K YAGA, RJ REITER

      NEUROSCIENCE LETTERS184 ( 2 ) 109 - 112   1995年1月

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      記述言語:英語   出版者・発行元:ELSEVIER SCI PUBL IRELAND LTD  

      The in vitro effect of melatonin on the release of luteinizing hormone (LH) and follicle stimulating hormone (FSK) from fetal rat pituitary cells was investigated. A significant inhibition of LH release induced by 10(-9) M luteinizing hormone releasing hormone (LHRH) was seen when cells were incubated with 10(-9) M melatonin. FSH release was unaffected by either LHRH alone or LHRH in combination with melatonin. In addition, the significant inhibitory effect of melatonin was reduced by pretreatment of the pituitary cells with 10(-10) M melatonin. These findings indicate that melatonin can act directly on the fetal pituitary gland to suppress LHRH-induced release of LH perhaps by a mechanism which eventually involves down-regulation of the melatonin receptors.

      DOI: 10.1016/0304-3940(94)11181-H

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    • IDENTIFICATION OF MELATONIN IN DIFFERENT ORGANS OF THE CRICKET, GRYLLUS-BIMACULATUS

      MT ITOH, A HATTORI, Y SUMI, T SUZUKI

      ZOOLOGICAL SCIENCE11 ( 4 ) 577 - 581   1994年8月

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      記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

      The possible presence of melatonin (N-acetyl-5-methoxytryptamine) was investigated in different tissues and organs of adult crickets by the use of reversed-phase high-performance liquid chromatography with fluorometric detection and radioimmunoassay. Melatonin was detected in the compound eye, antenna, ovipositor, palp, cercus, leg, wing, brain, ovary, digestive tube and Malpighian tube of the crickets, indicating that melatonin was very widely distributed in the crickets. The results suggest that melatonin in these organs might be involved in the local control of various aspects of rhythmic activity.

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    • CHARACTERISTICS, DAY-NIGHT CHANGES, SUBCELLULAR-DISTRIBUTION AND LOCALIZATION OF MELATONIN BINDING-SITES IN THE GOLDFISH BRAIN

      M IIGO, M KOBAYASHI, R OHTANIKANEKO, M HARA, A HATTORI, T SUZUKI, K AIDA

      BRAIN RESEARCH644 ( 2 ) 213 - 220   1994年5月

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      記述言語:英語   出版者・発行元:ELSEVIER SCIENCE BV  

      Melatonin binding sites in the goldfish brain were characterized by radioreceptor assay using 2-[I-125]iodomelatonin as the radioligand. Specific binding of 2-[I-125]iodomelatonin was rapid, stable, saturable and reversible. Saturation experiments demonstrated that 2-[I-125]iodomelatonin binds to a single class of receptor site with an affinity constant (K-d) of 29.8 +/- 0.7 pM and a total binding capacity (B-max) of 11.47 +/- 0.33 fmol/mg protein at mid-light. At mid-dark, the B-max value decreased significantly to 7.90 +/- 0.23 fmol/mg protein (P &lt; 0.01) with no significant variation in the Kb value(33.8 +/- 1.5 pM). Competition experiments revealed the following order of pharmacological affinities: 2-iodomelatonin &gt; melatonin &gt; 6-hydroxymelatonin &gt; N-acetyl-5-hydroxytryptamine &gt; 5-methoxytryptamine &gt; 5-methoxytryptophol &gt; 5-methoxyindole-3-acetic acid. 5-Hydroxytryptamine, 5-hydroxytryptophol, 5-hydroxyindole-3-acetic acid, norepinephrine and acetylcholine exhibited no inhibition. Subcellular distribution of melatonin binding sites was demonstrated to be greatest in the P-2 and P-3 fractions as compared with the P-1 fraction. Localization of melatonin binding sites in discrete brain areas was determined to be highest in the optic tectum-thalamus and hypothalamus, intermediate in the telencephalon, cerebellum and medulla oblongata, and lowest in the olfactory bulbs and pituitary gland. These results suggest that characteristics of melatonin receptors are highly conserved during evolution and that in this species melatonin plays neuromodulatory roles in the central nervous system through specific receptors.

      DOI: 10.1016/0006-8993(94)91682-9

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    • THE PINEAL MELATONIN RHYTHM AND ITS REGULATION BY LIGHT IN A SUBTERRANEAN RODENT, THE VALLEY POCKET GOPHER (THOMOMYS BOTTAE)

      RJ REITER, MN REITER, A HATTORI, K YAGA, DC HERBERT, L BARLOWWALDEN

      JOURNAL OF PINEAL RESEARCH16 ( 3 ) 145 - 153   1994年4月

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      記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      The daytime and nighttime levels of pineal N-acetyltransferase (NAT) activity, hydroxyindole-O-methyltransferase (HIOMT) activity, and melatonin were measured in adult male and female valley pocket gophers, Thomomys bottae. This species was chosen for study because it is a subterranean rodent that inhabits burrows whose openings to the surface are closed. Therefore, under field conditions it is estimated that the pocket gopher spends roughly 99% of its time in absolute darkness in underground burrows. When wild captured pocket gophers were maintained under a light:dark cycle (light intensity during the day of roughly 140 mu W/cm(2)), nighttime levels of pineal NAT activity and melatonin content were higher than values measured during the day; on the other hand, HIOMT activity in the pineal gland was similar in the day and at night. When pocket gophers were exposed to an extended light period (220 mu W/cm(2)) 4 hr into the night, the rise in melatonin synthesis normally associated with darkness onset was not inhibited. Also, when gophers were acutely exposed to a light intensity of 400 mu W/cm(2) for 1 hr beginning 4 hr after darkness onset, neither high nocturnal levels of pineal NAT nor pineal melatonin contents were reduced. Finally, when pocket gophers were exposed to a 600 mu W/cm(2) light intensity at either 4 hr or 8 hr into the dark period, pineal melatonin synthesis remained elevated at a level comparable to that measured in dark-exposed controls. The results show that under controlled laboratory conditions the pineal gland of the valley pocket gopher, a species that in its natural habitat spends about 99% of its time in absolute darkness, exhibits higher melatonin synthesis during night than during the day. While the rhythm in pineal melatonin production in the pocket gopher is clearly synchronized by the prevailing light:dark cycle, high nighttime pineal melatonin synthesis is not readily inhibited by light in the intensity range of 220 to 600 mu W/cm(2). In terms of its relative insensitivity to light at night, the pineal gland of the valley pocket gopher resembles that of other diurnally active rodents.

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    • 366 ラット松果体器官培養によるメラトニン分泌動態に関する研究

      房間 茂由, 石塚 文平, 萩庭 一元, 大野 祐子, 石田 徳人, 雨宮 章, 服部 淳彦, 飯郷 雅之, 鈴木 卓朗

      日本産科婦人科學會雜誌46   "S - 310"   1994年2月20日

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      出版者・発行元:社団法人日本産科婦人科学会  

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    • 妊娠および授乳ラットにおけるGnRH associated peptideのプロラクチン放出抑制効果-reverse hemolytic plaque assayよる解析-

      聖マリアンナ医大誌22   397 - 406   1994年

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    • 下垂体腺腫の機能病理学的手枝(1)-Reverse hemolytic plaque assay法-

        31   255 - 258   1994年

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    • Concentration of melatonin and enzymes of its biosynthesis of the epiphysis of silver fox male and females.

      Collection of the Institute of Cytology and Genetics : Endocrinology and Reproduction of fur Bearing Animals.2   76 - 83   1994年

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    • The circadian dynamics of the melatonin concentration in silver fox pineal gland, plasma and retina.

      Sechenov Physiol. J.80   38 - 41   1994年

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    • Peculiartites of melatonin biosynthesis in the pineal gland of relatively wild and domesticated silver foxes, Vulpes fulvus.

      J. Evol. Biochem. Physiol.30   338 - 343   1994年

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    • EFFECTS OF TESTOSTERONE AND LUTEINIZING-HORMONE-RELEASING HORMONE ON OCCURRENCE OF UBIQUITIN IN THE NUCLEUS OF RAT LH CELLS IN-VITRO

      R OHTANIKANEKO, Y WADA, A HATTORI, K TAKADA, M HARA, M IIGO, K IMAI, T SUZUKI

      ENDOCRINE2 ( 1 ) 69 - 73   1994年1月

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      記述言語:英語   出版者・発行元:HUMANA PRESS INC  

      Effects of testosterone (T) and LHRH on the occurrence of immunoreactive ubiquitin in the nucleus of rat LH cells were investigated in cell culture. The proportion of LH cells with ubiquitin-immunoreactive nuclei (%LH-nucUb) was significantly higher in cells from castrated rats than in those from intact rats. The increase in %LH-nucUb after castration was suppressed by T (1 x 10(-8) M) treatment for three days in vitro. Short repeated exposure to LHRH (10(-9) M for 10 min x 3) for 3 h did not affect the %LH-nucUb in either intact or castrated groups. In contrast, alternating prolonged exposure to LHRH (10(-9) M for 1 h and 10(-11) M for 3 h) for 60 h increased the %LH-nucUb in cells from intact rats, but not in cells from castrated rats. These results indicate that T decreases the occurrence of ubiquitin in the nucleus of activated LH cells, whereas LHRH increases it in less-activated LH cells, suggesting the involvement of ubiquitin in activation of LH cells. The present study also indicates that T and LHRH directly affect pituitary cells on the occurrence of ubiquitin in the nucleus of LH cells.

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    • PHOTOTRANSDUCTION-RELATED CIRCADIAN CHANGES IN INDOLEAMINE METABOLISM IN THE CHICK PINEAL-GLAND IN-VIVO

      JH SUN, RJ REITER, A HATTORI, K YAGA, DC HERBERT, ATC TSIN

      JOURNAL OF PINEAL RESEARCH15 ( 3 ) 132 - 137   1993年10月

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      記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      The purpose of this study was to examine the day/night levels of pineal melatonin and its rate limiting enzyme N-acetyltransferase (NAT) in relationship to the ratio of 11-cis- to all-trans-retinal. Three-week-old chicks were placed in 12:12 light:dark (LD 12:12) cycle for one week, pineals were collected during the light phase at 1500 (i.e., after 10 hr light), during the dark phase at 1900 (i.e., 2 hr after dark), at 2100 (i.e., 4 hr after dark), and at 2300 (i.e., 6 hr after dark) and after light extension to 1900. The results show that light-sensitive 11-cis-retinal in the chick pineal has the same diurnal rhythm as NAT and melatonin; all constituents increased within 2, hr of darkness onset (at 1900) and reached their peak after 4 hr of dark. All values were lowest during the light phase at 1500. Low values for 11-cis-retinal, NAT, and melatonin were also seen in the group of chicks which experienced light extension to 1900. The data indicate that in vivo light plays a major role in triggering rhodopsin-bound 11-cis-retinal production within 2-4 hr after darkness onset; this change likely serves as the signal for the subsequent formation of the hormonal product of the pineal gland, melatonin.

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    • UNUSUAL RESPONSES OF NOCTURNAL PINEAL MELATONIN SYNTHESIS AND SECRETION TO SWIMMING - ATTEMPTS TO DEFINE MECHANISMS

      K YAGA, DX TAN, RJ REITER, LC MANCHESTER, A HATTORI

      JOURNAL OF PINEAL RESEARCH14 ( 2 ) 98 - 103   1993年3月

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      記述言語:英語   出版者・発行元:MUNKSGAARD INT PUBL LTD  

      The effect of swimming at night on rat pineal melatonin synthesis was compared with that of light exposure at night. Rats were forced to swim at 0030 hr (lights out at 2000 hr) and sacrificed by decapitation 15 and 30 min later, immediately after swimming. Other groups of animals were exposed to white light (650 muW/cm2) for 15 and 30 min at same time. Swimming caused a rapid and highly significant drop in the melatonin content in the pineal gland; however, the activity of N-acetyltransferase (NAT), the supposed rate limiting enzyme in the melatonin production, was not changed. Despite the drop in pineal melatonin levels, serum concentrations of the indole remained elevated in the rats that swam. In contrast, melatonin levels in the pineal and serum of light exposed rats fell precipitously, accompanied by a significant suppression of NAT activity. Since we anticipated that the strenuous exercise associated with swimming may induce release of artrial natriuretic peptide (ANP) from the heart, which in turn could cause the release of pineal melatonin, in a second study we injected physiological saline intravenously to stretch the cardiac muscle and release ANP. Three milliliters of normal saline was injected during the day into the jugular vein of anesthetized rats that were pretreated with isoproterenol to stimulate pineal melatonin production. Animals were killed 15 min after the saline injection, and pineal NAT activity and pineal melatonin levels were measured. The saline injections caused no alteration in the elevated levels of either NAT or melatonin. These data suggest that the disparity in pineal NAT activity (which was high) and pineal melatonin (which was low), in animals swum at night, may not be caused by ANP which is released during strenuous exercise such as swimming.

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    • 培養血管内皮細胞の線溶反応におけるtype(]G0004[)コラーゲンの影響

      聖マリアンナ医大誌21   1209 - 1212   1993年

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    • 血管内皮細胞からのPAI-1遊離抑制-高分子蛋白分解酵素阻害薬による基礎的検討-

      聖マリアンナ医大誌21   268 - 270   1993年

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    • 個々の下垂体細胞におけるエキソサイトーシスによるホルモン放出量の解析

      生理研年報14   219 - 220   1993年

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    • 再閉塞におけるPAI-1の意義とその抑制法の検討-培養血管内皮細胞の反応について-

      医学と薬学29   73 - 76   1993年

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    • QUANTITATIVE MORPHOLOGICAL ANALYSIS OF THE PITUITARY-GLAND IN PROTEIN-CALORIE MALNOURISHED RATS

      DC HERBERT, T YASHIRO, T MURAKI, T OKANO, A HATTORI, T SUZUKI

      ANATOMICAL RECORD235 ( 1 ) 121 - 125   1993年1月

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      記述言語:英語   出版者・発行元:WILEY-LISS  

      A quantitative analysis of the pituitary gland was conducted to ascertain the effects of protein-calorie malnutrition on the morphology of the somatotrophs, gonadotrophs, thyrotrophs, and corticotrophs. Male rats were fed a low, 8% protein diet from 20 to 50 days of age, while their age-matched controls were given a diet containing 27% protein. The hypophyses were then processed for light microscopic immunocytochemical staining using antibodies to growth hormone, the beta subunits of luteinizing hormone and thyroid stimulating hormone, and adrenal corticotrophic hormone. The number of each cell type along with an evaluation of the cell, cytoplasmic, and nuclear areas was conducted using a computerized image analyzer. All of these parameters were reduced significantly in the somatotrophs as a result of the low protein diet, while in the gonadotrophs, the cell, cytoplasmic, and nuclear areas were similarly affected. Smaller cell number, cell area, and nuclear area were noted in the corticotrophs of the malnourished animals, while in the thyrotrophs, only the cell and nuclear areas were reduced. The data demonstrate that each pituitary cell type responds in a unique manner to undernutrition.

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    • In vivo and in vitro response of the hypophysis of protein-calorie malnourished rats to luteinizing hormone releasing hormone.

      Biomed. Res.4   215 - 226   1993年

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    • Melatonin content in tissues of relatively wild and domesticated silver fox Vulpes fulvus.

      J. Evol. Biochem. Physiol.29   482 - 486   1993年

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    • INCREASE IN UBIQUITIN-IMMUNOREACTIVE NUCLEI IN RAT PITUITARY LUTEINIZING-HORMONE CELLS AFTER CASTRATION

      R OHTANIKANEKO, A HATTORI, M HARA, M IIGO, K TAKADA, T SUZUKI

      ENDOCRINOLOGY130 ( 5 ) 2545 - 2548   1992年5月

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      記述言語:英語   出版者・発行元:ENDOCRINE SOC  

      Immunocytochemical detection of ubiquitin in the nucleus of rat LH cells and the effects of castration and testosterone replacement on the occurrence of immunoreactive ubiquitin in the nucleus were investigated. Immunoreactive ubiquitin occurred in certain nuclei, mostly belonging to identified LH cells. The concentration of testosterone in blood was altered by castration and implantation of testosterone into castrated rats, and the occurrence of ubiquitin was examined weekly for the following 4 weeks. In castrated rats, the proportion of LH cells with ubiquitin-immunoreactive nuclei was high throughout the experiment. In castrated rats implanted with testosterone, on the contrary, the proportion remained significantly lower. Ubiquitin may be involved in the cellular activity of LH cells in the rat pituitary.

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    • 蛋白分解酵素阻害薬Gabexate Mesilate添加による培養血管内皮細胞からのPAI-1放出抑制の検討

      今原佐知子

      聖マリアンナ医大誌20   134 - 139   1992年

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    • 組織プラスミノーゲンアクチベーター(tPA)添加による培養血管内皮細胞からのインヒビター(PAI-1)遊離

      鴨川旭

      聖マリアンナ医大誌20   214 - 217   1992年

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    • Reverse hemolytic plaque assayを用いた下垂体線腫の解析

      病理と臨床10   988 - 993   1992年

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    • 雄ラット個々の下垂体細胞におけるLHRH刺激後のLH放出動態-plaque assayによる解析-

      友池 英樹, 服部 淳彦, 松田 仁

      聖マリアンナ医大誌20 ( 6 ) 1062 - 1070   1992年

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      記述言語:日本語   出版者・発行元:聖マリアンナ医科大学医学会  

      1個の細胞からのホルモン放出量を経時的に測定することができるreverse hemolytic plaque assayの変法(continuous plaque assayとsequential plaque assay)を用い,LHRH刺激後のLH放出動態を個々の細胞で調べた。用いた動物は60日齢のWistar-Imamichi系の雄ラットで,摘出した下垂体前葉を酵素によりsingle cellsに解離し,24時間培養後,10-9MのLHRHを添加して2種類のassayを行った。大きく分類すると4種類の放出パターンが観察された。すなわち,第1は,LHRH刺激後急速にLHを放出し,その後ほとんど放出しない細胞群,第2は,徐々にLHの放出量を増加させる細胞群,第3は,LHRHで刺激しているにもかかわらず一定時間が経過しないと放出を開始しない細胞群,第4は,培養時間(7時間)内ではまったくLHを放出しない細胞群である。以上の結果より,同じLH細胞といっても個々の細胞でLHRHに対する反応性が異なることが明らかになった

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    • REVERSE HEMOLYTIC PLAQUE-ASSAY - ELECTRON-MICROSCOPIC OBSERVATION OF PLAQUE-FORMING SINGLE ADENOMA CELLS IN GH PRODUCING ADENOMAS

      S YAMADA, T AIBA, A HATTORI, T SUZUKI, SL ASA, K KOVACS

      PATHOLOGY RESEARCH AND PRACTICE187 ( 5 ) 546 - 551   1991年6月

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      記述言語:英語   出版者・発行元:GUSTAV FISCHER VERLAG  

      In this study, 3 GH-producing adenomas were studied as materials to clarify the usefulness of electron microscopic investigation of plaque-forming single adenoma cells, and ultrastructural morphometric comparisons were made to determine whether some differences exist at the cytoplasmic organelle level between small plaque-forming cells and large plaque-forming cells. In two cases, the relative cytoplasmic volume density of Golgi apparatus and lysosomes were significantly greater and the diameter of seretory granules were significantly smaller in large plaque-forming cells compared to small plaque-forming cells. Moreover, in one of them, not only the diameter but also the cytoplasmic volume density of secretory granules were significantly smaller in large plaque-forming cells. These findings suggest a greater secretory granule synthesis and internal granule destruction, and faster granules secretion at an early stage of formation in large plaque-forming cells compared with small plaque-forming cells. It became apparent through this study that electron microscopic investigation of plaque-forming single cells is practically useful since plasma membrane any cytoplasmic organelles were preserved well after processing for EM in most plaque-forming cells.

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    • 蛋白分解酵素阻害薬による血管内皮細胞由来線溶因子放出制御の検討

      聖マリアンナ医大誌19   343 - 346   1991年

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    • 黄体形成ホルモン放出ホルモンの比較内分泌学

      ペプチドホルモン ホルモンの生物科学10,学会出版センター (東京)   109 - 140   1991年

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    • Rythms of blood melatonin in individual Japanese quail(Coturnix coturnix japonica).

      St. Marianna Med. J.18   241 - 248   1990年

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    • 唾液腺Receptor,(]G0004[).細胞,組織別レセプター論.

      日本臨床47   767 - 770   1989年

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    • FINE-STRUCTURAL HETEROGENEITY OF PRL CELLS IN THE RAT ANTERIOR-PITUITARY GLAND - ITS RELATIONSHIP TO HORMONE-SECRETION

      T YASHIRO, A HATTORI, T SUZUKI

      ANATOMICAL RECORD220 ( 4 ) A107 - A107   1988年4月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:WILEY-LISS  

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    • ラット下垂体前葉における細胞間相互作用の証明に関する形態学的アプローチ

      河野研研究年報38   33 - 42   1988年

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    • 個々の細胞からのホルモン放出量測定法-reverse hemolytic plaque assay-

      生体の科学39   510 - 512   1988年

      詳細を見る

    • Functional and morphological heterogeneity of prolactin cells in the rat.

      Kyoto Prolaction Conference Monograph3.   61 - 70   1988年

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    • EFFECTS OF INTRACRANIALLY IMPLANTED CHOLECYSTOKININ AND SUBSTANCE-P ON SERUM CONCENTRATIONS OF GONADOTROPINS, PROLACTIN AND THYROID STIMULATING HORMONE IN THE RAT

      H UEMURA, A HATTORI, M WADA, H KOBAYASHI

      ZOOLOGICAL SCIENCE4 ( 2 ) 331 - 337   1987年4月

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      記述言語:英語   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • Fine structural and immunohistochemical identification of TSH and LH cells in the C57BL mouse anterior pituiary gland.

      Jikeikai Med. J.34   479 - 487   1987年

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    • CIRCADIAN VARIATION IN MELATONIN RELEASE FROM THE EYE OF JAPANESE-QUAIL

      A HATTORI, M WADA, Y SONODA

      ZOOLOGICAL SCIENCE3 ( 6 ) 1087 - 1087   1986年12月

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • EFFECTS OF 2 KINDS OF CHICKEN LUTEINIZING-HORMONE-RELEASING HORMONE (LH-RH), MAMMALIAN LH-RH AND ITS ANALOGS ON THE RELEASE OF LH AND FSH IN JAPANESE-QUAIL AND CHICKEN

      A HATTORI, S ISHII, M WADA

      GENERAL AND COMPARATIVE ENDOCRINOLOGY64 ( 3 ) 446 - 455   1986年12月

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      記述言語:英語   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

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    • DIFFERENT MECHANISMS CONTROLLING FSH AND LH-RELEASE IN JAPANESE-QUAIL (COTURNIX-COTURNIX JAPONICA) - EVIDENCE FOR AN INHERENTLY SPONTANEOUS RELEASE AND PRODUCTION OF FSH

      A HATTORI, S ISHII, M WADA

      JOURNAL OF ENDOCRINOLOGY108 ( 2 ) 239 - 245   1986年2月

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      記述言語:英語   出版者・発行元:J ENDOCRINOLOGY LTD  

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    • Actions of two intrinsic LHRHs on release of gonadotropins in quail : deferent structures but the same response.

      Pars Distalis of the Pituitary Gland. Yoshimura, F. and Gorbman, A. (eds)Elsevier   231 - 237   1986年

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    • INITIAL PROCESS OF PHOTOSTIMULATED LH-SECRETION IN QUAIL - FROM EPISODIC RELEASE TO CONSTANT SECRETION

      M WADA, A HATTORI, F HATANAKA

      ZOOLOGICAL SCIENCE2 ( 6 ) 982 - 982   1985年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • EFFECTS OF CHICKEN LHRH ON LH-RELEASE FROM QUAIL PITUITARY INVITRO BY VARIOUS MODES OF ITS APPLICATION WITH DIFFERENT FREQUENCIES, DURATIONS AND DOSES

      A HATTORI, S ISHII, M WADA

      ZOOLOGICAL SCIENCE2 ( 6 ) 982 - 982   1985年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • EFFECTS OF CHICKEN (GLN8)-LUTEINIZING AND MAMMALIAN (ARG8)-LUTEINIZING HORMONE-RELEASING HORMONES ON THE RELEASE OF GONADOTROPINS INVITRO AND INVIVO FROM THE ADENOHYPOPHYSIS OF JAPANESE QUAIL

      A HATTORI, S ISHII, M WADA, K MIYAMOTO, Y HASEGAWA, M IGARASHI, S SAKAKIBARA

      GENERAL AND COMPARATIVE ENDOCRINOLOGY59 ( 1 ) 155 - 161   1985年

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      記述言語:英語   出版者・発行元:ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS  

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    • ANNUAL CHANGES IN PLASMA GONADOTROPINS AND SEX STEROID IN THE WHITE IBIS IN CAPTIVITY

      S ISHII, A HATTORI, H SAKAI, S WAKABAYASHI, M WADA

      ZOOLOGICAL SCIENCE1 ( 6 ) 974 - 974   1984年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • EFFECTS OF MET-ENKEPHALIN AND CHOLECYSTOKININ (CCK4) ON SERUM LH AND PRL CONCENTRATIONS

      H UEMURA, M WADA, A HATTORI, H KOBAYASHI

      ZOOLOGICAL SCIENCE1 ( 6 ) 979 - 979   1984年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • STIMULATION OF FSH AND LH RELEASES BY 2 CHICKEN LHRHS AND MAMMALIAN LHRH INVITRO AND INVIVO

      A HATTORI, S ISHII

      JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY20 ( 6B ) 1548 - 1548   1984年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

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    • RECEPTIVITY OF QUAIL ADENOHYPOPHYSIS TO VARIOUS LUTEINIZING-HORMONE-RELEASING HORMONES (LHRHS)

      A HATTORI, S ISHII, K MIYAMOTO, Y HASEGAWA, M IGARASHI, M WADA

      ZOOLOGICAL SCIENCE1 ( 6 ) 974 - 974   1984年

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      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:ZOOLOGICAL SOC JAPAN  

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    • A DIFFERENT CONTROL MECHANISM IN FSH AND LH SECRETIONS OF QUAIL

      A HATTORI, S ISHII, M WADA

      JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY20 ( 6B ) 1548 - 1548   1984年

      詳細を見る

      記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD  

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    • Avian Gonadotropin-Releasing Hormones

      Ishii Susumu, Hattori Atsuhiko, Wada Masaru, MIyamato Kaoru, Hasegawa Yoshihisa, Igarashi Masao

      Gunma symposia on endocrinology21   79 - 91   1984年

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      記述言語:英語   出版者・発行元:群馬大学  

      CiNii Article

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    • LHRHの分子進化と種特異性

      服部 淳彦

      化学と生物21 ( 9 ) 567 - 568   1983年

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      記述言語:日本語   出版者・発行元:学会出版センタ-  

      CiNii Article

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      その他リンク: http://search.jamas.or.jp/link/ui/1984081453

    • 培養血管内皮細胞の線溶系応答からみたt-PA/UK併用の利点

      医学と薬学30   1131 - 1133  

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    ▼全件表示

    書籍等出版物

    • 自然界におけるメラトニンの分布

      メラトニン研究の最新の進歩  2004年 

      詳細を見る

    • 脳内物質メラトニン

      朝日出版社  1996年 

      詳細を見る

    • 奇跡のホルモン メラトニン

      講談社(監修)  1995年 

      詳細を見る

    講演・口頭発表等

    • マウスにおける内因性メラトニン代謝産物N-acetyl-5-methoxykynuramine(AMK)と海馬における糖取込みとの関連.

      張賢, 丸山雄介, 岩下洸, 服部淳彦

      日本動物学会関東支部第71回大会  2019年3月9日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • メラトニン(MEL)はキンギョの脳における糖の取込みを促進する.

      岡田玲那, 渡辺数基, 丸山雄介, 服部淳彦

      日本動物学会関東支部第71回大会  2019年3月9日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • キンギョの鱗においてノルアドレナリン(NA)の刺激で合成されるN-acetyl-5-methoxykynuramine(AMK)は骨芽細胞を活性化する.

      河村平, 小泉なぎさ, 丸山雄介, 服部淳彦

      日本動物学会関東支部第71回大会  2019年3月9日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • メラトニン研究の最新情報 招待有り

      服部淳彦

      日本抗加齢医学会2018年度 第4回メディアセミナー  2019年2月18日 

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      記述言語:日本語   会議種別:メディア報道等  

      開催地:東京  

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    • ゼブラフィッシュ鱗を用いた骨折修復過程におけるライブイメージング解析.

      小林静静, 近藤真央, 山森汐莉, 鈴木信雄, 服部淳彦, 北村敬一郎, 山口正晃, 小林功

      日本分子生物学会第41回年会  2018年11月28日 

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      記述言語:日本語  

      開催地:横浜  

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    • 長期記憶誘導作用を持つメラトニンの脳内代謝産物AMK 招待有り

      服部淳彦

      第28回日本臨床精神神経薬理学会・第48回日本神経精神薬理学会 合同年会  2018年11月16日 

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      記述言語:日本語   会議種別:口頭発表(招待・特別)  

      開催地:東京  

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    • マウスの長期記憶形成に関与する内因性のメラトニン代謝産物 N-acetly-5- methoxykynuramine (AMK).

      岩下洸, 丸山雄介, 張賢, 松本幸久, 千葉篤彦, 服部淳彦

      第43回日本比較内分泌学会大会及びシンポジウム  2018年11月9日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • 夜間のメラトニンはインスリンを介さずにキンギョの糖代謝を調節している.

      渡辺数基, 中野真樹, 丸山雄介, 服部淳彦

      第43回日本比較内分泌学会大会及びシンポジウム  2018年11月9日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • α-MSH は血漿カルシトニン濃度を上昇させてカルシウム代謝に関与する.

      鈴木信雄, 五十里雄大, 小林勇喜, 水澤寛太, 高橋明義, 木谷洋一郎, 関口俊男, 遠藤雅人, 神戸川明, 朝比奈潔, 田渕圭章, Thumronk Amornsakun, 服部淳彦

      第43回日本比較内分泌学会大会及びシンポジウム  2018年11月9日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:仙台  

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    • メラトニンと睡眠・抗加齢効果 招待有り

      服部淳彦

      日本抗加齢協会セミナー  2018年9月23日 

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      記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

      開催地:フジテレビ1階マルチシアター  

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    • Effects of low-intensity pulsed ultrasound on osteoclastogenesis with goldfish scales having both osteoclasts and osteoblasts

      Nobuo Suzuki, Taizo Hanmoto, Yoshiaki Tabuchi, Sachiko Yano, Mika Ikegame, Kei-ichiro Kitamura, Toshio Sekiguchi, Isao Kobayashi, Azusa Seki, Atsuhiko Hattori

      日本宇宙生物科学会第32回大会  2018年9月21日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • 骨粗鬆症モデルラットにおける副甲状腺ホルモンとメラトニンの併用効果

      池亀美華, 田中みか子, 江尻貞一, 服部淳彦, 高尾亮子, 内部健太, 岡村裕彦

      第60回歯科基礎医学会総会  2018年9月5日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニンMT1/MT2受容体アゴニストによる学習記憶機能の増強作用

      佐野真広, 岩下洸, 梅木智裕, 中村華子, 吉川史紘, 服部淳彦, 千葉篤彦

      第29 回 日本行動神経内分泌研究会  2018年9月5日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • アルツハイマー様の学習記憶障害を示すマウスに対するメラトニンの学習記憶機能の改善効果

      梅木智裕, 岩下洸, 佐野真広, 中村華子, 吉川史紘, 服部淳彦, 千葉篤彦

      第29 回 日本行動神経内分泌研究会  2018年9月5日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • マウスにおける内因性のメラトニン代謝産物N-acetly-5-methoxykynuramine (AMK)と長期記憶形成との関連

      岩下洸, 丸山雄介, 張賢, 松本幸久, 千葉篤彦, 服部淳彦

      第29回日本行動神経内分泌研究会  2018年9月5日 

      詳細を見る

      記述言語:日本語   会議種別:口頭発表(一般)  

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    • 赤外・ラマン分光による硬骨魚ウロコの構造解析

      奈良雅之, 丸山雄介, 服部淳彦

      第27回バイオイメージング学会学術集会  2018年9月3日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • 「学習・記憶」-加齢性記憶障害の回復作用 招待有り

      服部淳彦

      第10回抗加齢内分泌研究会  2018年9月2日 

      詳細を見る

      記述言語:日本語   会議種別:口頭発表(招待・特別)  

      開催地:東京  

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    • Melatonin, the hormone of gloom: Implications in the semilunar-synchronized spawning of the grass puffer. 国際会議

      Hironori Ando, Md. Shahjahan, Takashi Kitahashi, Atsuhiko Hattori

      AOSCE2018 University of Sydney  2018年7月8日 

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      記述言語:英語   会議種別:口頭発表(一般)  

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    • 内因性のメラトニン代謝産物N-acetyl-5-methoxykyuramine(AMK)による長期記憶誘導作用

      岩下光, 松本幸久, 丸山雄介, 張賢, 千葉篤彦, 服部淳彦

      第18回日本抗加齢医学会総会  2018年5月25日 

      詳細を見る

      記述言語:日本語   会議種別:口頭発表(一般)  

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    • 象牙質や象牙質結晶に対するメラトニンの影響

      三島弘幸, 服部淳彦, 鈴木信雄, 松本 敬, 池亀美華, 見明康雄, 松本由樹

      第123回日本解剖学会総会・全国学術集会  2018年3月28日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • 魚類の骨代謝に対する黒色素胞刺激ホルモンの影響:in vivo及びin vitroによる解析

      鈴木信雄, 石津偉統, 木谷洋一郎, 五十里雄大, 関口俊男, 北村敬一郎, 遠藤雅人, 服部淳彦, 水澤寛太, 高橋明義

      平成30年度日本水産学会春季大会  2018年3月26日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • カイコガ幼虫と成虫の頭部におけるメラトニン合成経路関連物質の昼夜変化

      古屋瑠菜, 丸山雄介, 服部淳彦

      日本動物学会関東支部 第70回大会  2018年3月21日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • フッ素の硬骨魚類の骨代謝に与える影響

      鈴木信雄, 佐藤将之, 谷内口孝治, 木谷洋一郎, 関口俊男, 五十里雄大, 小木曽正造, 田渕圭章, 三島弘幸, 見明康雄, 本橋慶一, 矢口行雄, 服部淳彦

      第2回富山湾研究会  2018年3月5日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • α-melanocyte-stimulating hormone functions to calcium metabolism in goldfish. 国際会議

      Ishizu, H, Sekiguchi, T, Ikari, T, Kitamura, K, Kitani, Y, Endo, M, Urata, M, Hattori, A, Mizusawa, K, Takahashi, A, Suzuki, N

      International Symposium “Environmental researches in northern Japan Sea and related regions”  2018年3月3日 

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      記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

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    • Plasma cortisol levels decreases in marine fishes kept in deep ocean water. 国際会議

      Ikari, T, Kitani, Y, Ogiso, S, Sekiguchi, T, Toyohara, C, Nakayama, Y, Maruyama, Y, Hattori, A, Tabuchi, Y, Kambegawa, A, Asahina, K, Fukushi, K, Suzuki, N

      International Symposium “Environmental researches in northern Japan Sea and related regions”  2018年3月3日 

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      記述言語:英語   会議種別:シンポジウム・ワークショップ パネル(公募)  

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    • LIPUSに対する骨芽細胞及び破骨細胞の応答:キンギョのウロコを用いたin vitro及びin vivoの解析

      鈴木信雄, 半本泰三, 田渕圭章, 近藤 隆, 池亀美華, 北村敬一郎, 関口俊男, 小林 功, 関あずさ, 服部淳彦

      第21回超音波骨折治療研究会  2018年1月20日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニンの老化抑制効果 招待有り

      服部淳彦

      脳心血管抗加齢研究会2017  2017年12月16日 

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      記述言語:日本語   会議種別:口頭発表(招待・特別)  

      開催地:大阪  

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    • 老化抑制ホルモンとしてのメラトニン 招待有り

      服部淳彦

      日本抗加齢医学会-専門医・指導士認定委員会講習会  2017年12月10日 

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      記述言語:日本語   会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

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    • 軟骨組織におけるメラトニン合成とその受容体発現は慨日リズムを持ち、軟骨細胞の代謝に影響を及ぼす

      服部高子, FU Shanqi, 桑原実穂, 内田揺子, 近藤星, 林大智, 下村侑司, 高垣安紗美, 西田崇, 丸山雄介, 池亀美華, 服部淳彦, 久保田聡

      第90回日本生化学会大会  2017年12月7日 

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      記述言語:日本語  

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    • コオロギの長期記憶形成能の日内および概日変化と昼夜で異なるメラトニン脳内代謝産物の長期記憶誘導効果

      佐藤紀之, 松本幸久, 丸山雄介, 服部淳彦

      第42回日本比較内分泌学会大会  2017年11月18日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • メラトニン産生能を持つマウスにおけるメラトニン脳内代謝産物N-acetyl-5-methoxykyuramine(AMK)の長期記憶誘導作用

      岩下洸, 松本幸久, 丸山雄介, 張賢, 千葉篤彦, 服部淳彦

      第42回日本比較内分泌学会大会  2017年11月18日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • 黒色素胞刺激ホルモンはキンギョの再生ウロコの骨芽細胞と破骨細胞を活性化する

      石津偉統, 関口俊男, 五十里雄大, 木谷洋一郎, 北村敬一郎, 浦田 慎, 遠藤雅人, 服部淳彦, 水澤寛太, 高橋明義, 鈴木信雄

      第42回日本比較内分泌学会およびシンポジウム  2017年11月17日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • コオロギにおける長期記憶形成能の概日変化とメラトニン及びその脳内代謝産物の長期記憶誘導効果

      佐藤紀之, 松本幸久, 丸山雄介, 服部淳彦

      第24回時間生物学会学術大会  2017年10月28日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • 特発性正常圧水頭症患者の髄液排除試験後にみられた起床時の尿中メラトニン濃度の上昇

      Tamamongood Thiparpa, 丸山雄介, 山本信二, 大木純, 田畑均, 服部淳彦

      日本脳神経外科学会第76回学術総会  2017年10月12日 

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      記述言語:日本語   会議種別:ポスター発表  

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    • Effect of circadian rhythm synchronous factor melatonin on structure, composition, and calcification of dentin and odontoblasts. 国際会議

      Mishima, H, Tanabe, S, Hattori, A, Suzuki, N, Kakei, M, Matumoto, T, Ikegame, M, Miake, Y, Matsumoto, Y

      The 14th International Symposium on Biomineralization  2017年10月9日 

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      記述言語:英語   会議種別:ポスター発表  

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    • Effects of α-melanocyte-stimulating hormone on osteoblasts and osteoclasts in the regenerating scales of goldfish. 国際会議

      Ishizu, H, Sekiguchi, T, Ikari, T, Kitamura, K, Kitani, Y, Endo, M, Urata, M, Hattori, A, Mizusawa, K, Takahashi, A, Suzuki, N

      3rd International Symposium “International Collaboration Research Base for Reaction of Atmosphere-Marine-Ecosystem Caused by Aerosol”  2017年10月9日 

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      記述言語:英語   会議種別:ポスター発表  

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    • Stress response of Noto deep ocean water in marine fishes. 国際会議

      Ikari, T, Kitani, Y, Ogiso, S, Sekiguchi, T, Toyohara, C, Nakayama, Y, Maruyama, Y, Hattori, A, Tabuchi, Y, Kambegawa, A, Asahina, K, Fukushi, K, Suzuki, N

      3rd International Symposium “International Collaboration Research Base for Reaction of Atmosphere-Marine-Ecosystem Caused by Aerosol”  2017年10月9日 

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      記述言語:英語   会議種別:ポスター発表  

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    • 概説と学習・記憶-メラトニンの脳内代謝産物が持つ長期記憶誘導作用 招待有り

      服部淳彦

      日本動物学会第88回大会  2017年9月22日 

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      記述言語:日本語   会議種別:シンポジウム・ワークショップ パネル(指名)  

      開催地:富山  

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    • 記憶力が低下した加齢マウスにおけるメラトニン脳内代謝産物の長期記憶誘導作用

      岩下光, 松本幸久, 丸山雄介, 千葉篤彦, 服部淳彦

      日本動物学会第88回大会  2017年9月21日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニンによる長期記憶の誘導効果は投与時刻により異なる

      佐藤紀之, 松本幸久, 服部淳彦

      日本動物学会第88回大会  2017年9月21日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:富山  

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    • 赤外・ラマン分光による魚類ウロコの状態解析

      奈良雅之, 丸山雄介, 服部淳彦

      日本分析化学会  2017年9月12日 

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      記述言語:日本語  

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    • 就寝前のブルーライトカット眼鏡装用によりインスリン抵抗性が改善

      柳川達生, 綾木雅彦, 服部淳彦, 永井紀博, 根岸一乃, 坪田一男

      日本睡眠学会第42回定期学術集会  2017年6月29日 

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      記述言語:日本語   会議種別:ポスター発表  

      開催地:パシフィコ横浜  

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    • 概日リズム同調因子メラトニンによる象牙質や象牙芽細胞の組織構造への影響

      三島弘幸, 田辺咲貴, 服部淳彦, 鈴木信雄, 筧光男, 松本 敬, 池亀美華, 見明康雄, 松本由樹

      第35回化石研究会総会・学術大会  2017年6月3日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:福井県立恐竜博物館  

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    • ジヒドロキシインドールカルボン酸を用いたメラニン重合評価系の検討とプラセンタにおける美白有効成分の同定

      丸山雄介, 宮内大治, 横川剛, 服部淳彦

      第17回日本抗加齢医学会総会  2017年6月2日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニン代謝産物であるN-acetyl-5-methoxykyuramine(AMK)とその類似物質による長期記憶の誘導効果-コオロギをモデル動物として

      松本幸久, 丸山雄介, 松本千尋, 影近弘之, 松野弘幸, 岩下光, 千葉篤彦, 服部淳彦

      第17回日本抗加齢医学会総会  2017年6月2日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニンの脳内代謝産物であるN-acetyl-5-methoxykyuramine(AMK)による老齢マウスにおける長期記憶誘導作用

      岩下光, 松本幸久, 丸山雄介, 千葉篤彦, 服部淳彦

      第17回日本抗加齢医学会総会  2017年6月2日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:東京国際フォーラム, 東京  

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    • メラトニンによる象牙質や象牙芽細胞への影響

      三島弘幸, 田辺咲貴, 服部淳彦, 鈴木信雄, 松本 敬, 池亀美華, 見明康雄

      第122回日本解剖学会総会・全国学術集会  2017年3月28日 

      詳細を見る

      記述言語:日本語   会議種別:ポスター発表  

      開催地:長崎大学  

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    • 魚類の骨代謝に対する黒色素胞刺激ホルモンの影響

      鈴木信雄, 木谷洋一郎, 五十里雄大, 石津偉統, 小木曽正造, 関口俊男, 服部淳彦, 高橋明義

      平成29年度日本水産学会春季大会  2017年3月26日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:東京海洋大学品川キャンパス,東京都  

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    • Effects of mercury on osteoclasts and osteoblasts in the scales of the nibbler fish (marine teleost). 国際会議

      Yachiguchi, K, Sekiguchi, T, Hattori, A, Yamamoto, M, Kitamura, K, Tabuchi, Y, Suzuki, N

      Joint International Symposium Institute of Nature and Environmental Technology  2017年2月28日 

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      記述言語:英語   会議種別:口頭発表(一般)  

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    • Sodium fluoride acts on osteoclasts and osteoblasts and disrupts calcium metabolism in goldfish. 国際会議

      Sato, M, Hanmoto, T, Yachiguchi, K, Tabuchi, Y, Kondo, T, Endo, M, Kitani, Y, Sekiguchi, T, Urata, M, Srivastav, A.K, Mishima, H, Hattori, A, Suzuki, N

      Joint International Symposium Institute of Nature and Environmental Technology  2017年2月28日  Kanazawa University

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      記述言語:英語   会議種別:口頭発表(一般)  

      開催地:Ishikawa, Japan  

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    • Effect of sardine procalcitonin amino-terminal cleavage peptide on osteoblasts of the goldfish scales. 国際会議

      Kase, Y, Ikari, T, Sekiguchi, T, Sato, M, Kawada, T, Matsubara, S, Satake, H, Sasayama, Y, Endo, M, Hattori, A, Watanabe, T, Suzuki, N

      Joint International Symposium Institute of Nature and Environmental Technology  2017年2月28日  Kanazawa University

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      記述言語:英語   会議種別:口頭発表(一般)  

      開催地:Ishikawa, Japan  

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    • Deep ocean water influences on fish physiology: analysis of plasma cortisol levels. 国際会議

      Ikari, T, Kitani, Y, Sato, M, Ogiso, S, Toyohara, C, Hattori, A, Kambegawa, A, Asahina, K, Fukushi, K, Suzuki, N

      Joint International Symposium Institute of Nature and Environmental Technology  2017年2月28日  Kanazawa University

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      記述言語:英語   会議種別:口頭発表(一般)  

      開催地:Ishikawa, Japan  

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    • Low-intensity pulsed ultrasound moderately activates osteoclasts: Analysis with goldfish scales as a model of bone. 国際会議

      Hanmoto, T, Tabuchi, Y, Ikegame, M, Kondo, T, Kitamura, K, Endo, M, Mishima, H, Sekiguchi, T, Urata, M, Seki, A, Yano, S, Hattori, A, Suzuki, N

      Joint International Symposium Institute of Nature and Environmental Technology  2017年2月28日  Kanazawa University

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      記述言語:英語   会議種別:口頭発表(一般)  

      開催地:Ishikawa, Japan  

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    • メラトニンの脳内代謝産物であるN-acetyl-5-methoxykyuramine(AMK)のマウスにおける長期記憶誘導作用

      岩下光, 松本幸久, 丸山雄介, 千葉篤彦, 服部淳彦

      第 41回日本比較内分泌学会大及びシンポジウム  2016年12月9日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

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    • メラトニンによるインスリンを介さない血漿グルコース濃度低下作用-キンギョを用いて

      渡辺数基, 中野真樹, 服部淳彦

      第 41回日本比較内分泌学会大及びシンポジウム  2016年12月9日 

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      記述言語:日本語   会議種別:口頭発表(一般)  

      開催地:北里大学、神奈川  

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    • 繁殖後期のキンギョにおける血漿カルシウム濃度の低下と鱗で産生されるメラトニンとの関連

      松岡里沙, 丸山雄介, 鈴木信雄, 服部淳彦

      第 41回日本比較内分泌学会大及びシンポジウム  2016年12月9日 

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      記述言語:日本語   会議種別:ポスター発表  

      開催地:北里大学, 神奈川県  

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    • Melatonin metabolites rescue age-related momory impairment in the criket. 国際会議

      Yukihisa Matsumoto, Yusuke Maruyama, Chihiro Matsumoto, Hikaru Iwashita, Atsuhiko Chiba, Atsuhiko Hattori

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日  Okinawa Convention Center

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      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa, Japan  

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    • Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone. 国際会議

      Hanmoto, T, Tabuchi, Y, Ikegame, M, Kondo, T, Kitamura, K, Endo, M, Mishima, H, Sekiguchi, T, Urata, M, Seki, A, Yano, S, Hattori, A, Suzuki, N

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日  Okinawa Convention Center

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      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa, Japan  

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    • Suppressive effects of melatonin on osteoclastic function in chick calvaria. 国際会議

      Masaki Nakano, Mika Ikegame, Nobuo Suzuki, Atsuhiko Hattori

      2016年11月14日 

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      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa Convention Center, Okinawa, Japan  

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    • Effects of deep ocean water on fish physiology with special reference to stress hormones. 国際会議

      Ikari, T, Kitani, Y, Sato, M, Ogiso, S, Toyohara, C, Hattori, A, Kambegawa, A, Asahina, K, Fukushi, K, Suzuki, N

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日 

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      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa Convention Center, Okinawa, Japan  

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    • Sardine procalcitonin amino-terminal cleavage peptide is bioactive in osteoblasts of goldfish scales. 国際会議

      Kase, Y, Ikari, T, Sekiguchi, T, Sato, M, Kawada, T, Matsubara, S, Satake, H, Sasayama, Y, Endo, M, Hattori, A, Watanabe, T, Suzuki, N

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日 

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      記述言語:英語   会議種別:ポスター発表  

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    • Effects of sodium fluoride on calcium metabolism in goldfish, Carassius auratus. 国際会議

      Sato, M, Hanmoto, T, Yachiguchi, K, Tabuchi, Y, Kondo, T, Endo, M, Kitani, Y, Sekiguchi, T, Urata, M, Srivastav, A.K, Mishima, H, Hattori, A, Suzuki, N

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日 

      詳細を見る

      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa Convention Center, Okinawa, Japan  

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    • Both inorganic mercury and methylmercury influence on osteoclasts and osteoblasts in the scales of the marine teleost. 国際会議

      Yachiguchi, K, Sekiguchi, T, Hattori, A, Yamamoto, M, Kitamura, K, Tabuchi, Y, Suzuki, N

      The Joint Meeting of the 22nd International Congress of Zoology & the 87th meeting of the Zoological Society of Japan  2016年11月14日 

      詳細を見る

      記述言語:英語   会議種別:ポスター発表  

      開催地:Okinawa Convention Center, Okinawa, Japan  

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    • 睡眠を支えるメラトニン 招待有り

      服部淳彦

      日本抗加齢医学会北海道研究会  2016年10月30日 

      詳細を見る

      記述言語:日本語   会議種別:口頭発表(招待・特別)  

      開催地:札幌  

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    ▼全件表示

    所属学協会

    Works(作品等)

    • 内分泌撹乱物質の環境リスク

      2001年

      詳細を見る

    • 食素材としての植物由来の機能性成分の探索

      1998年
      -
      2000年

      詳細を見る

    • 各種食用植物におけるメラトニンの探索

      1996年
      -
      1998年

      詳細を見る

    共同研究・競争的資金等の研究

    • 高血糖を誘導した魚を用いた骨疾患の予防に関する研究:メラトニンを含む食品の効果

      日本学術振興会  科学研究費助成事業 

      鈴木 信雄, 小林 功, 服部 淳彦, 平山 順

      詳細を見る

      2023年4月 - 2026年3月

      課題番号:23K10933

      配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

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    • 長期記憶誘導効果をもつメラトニン代謝産物AMKの加齢変化と機能性食品への展開

      日本学術振興会  科学研究費助成事業 

      服部 淳彦, 渡辺 数基

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      2022年4月 - 2025年3月

      課題番号:22K11823

      配分額:4160000円 ( 直接経費:3200000円 、 間接経費:960000円 )

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    • 骨格筋における代謝異常・サルコペニア肥満と歯周炎の関わり-健康長寿を目指して-

      文部科学省/日本学術振興会 

      片桐 さやか, 鈴木 敏彦, 高橋 宏和, 廣田 朝光, 片桐 さやか, 服部 淳彦

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      2020年 - 2022年

      担当区分:連携研究者  資金種別:競争的資金

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    • 骨格筋における代謝異常・サルコペニア肥満と歯周炎の関わり-健康長寿を目指して-

      文部科学省/日本学術振興会 

      片桐 さやか, 鈴木 敏彦, 高橋 宏和, 廣田 朝光, 片桐 さやか, 服部 淳彦

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      2020年 - 2022年

      担当区分:連携研究者  資金種別:競争的資金

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    • 加齢に伴う長期記憶障害を回復させるメラトニン脳内代謝産物の効果と健康食品への展開

      文部科学省/日本学術振興会 

      服部 淳彦, 服部 淳彦

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      2018年 - 2020年

      担当区分:研究代表者  資金種別:競争的資金

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    • 黒色素胞刺激ホルモンの骨への新規作用:再生能力が高い硬組織(ウロコ)を用いた解析

      日本学術振興会  科学研究費助成事業 

      鈴木 信雄, 高橋 明義, 鈴木 徹, 服部 淳彦

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      2016年4月 - 2019年3月

      課題番号:16K07871

      配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

      本研究の目的は、魚類における黒色素胞刺激ホルモン(MSH)の骨代謝に対する新規作用を骨モデルであるウロコを用いて解析することである。本研究では、再生ウロコを用いて、MSHの骨代謝に対する作用をin vivo及びin vitroで解析を行い、MSHが破骨細胞及び骨芽細胞を活性化することを証明した。また、カルシウム代謝に関与しているカルシトニンの分泌をMSHが促していることもわかった。さらに、円鱗から櫛鱗への分化機構を調べるために、次世代シークエンス解析を行った。その結果、骨代謝関連遺伝子の制御機構の一端を明らかにすることができた。

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    • 光周期と異なるウズラの鳴声刺激による生殖リズムの制御機構

      日本学術振興会  科学研究費助成事業 

      産賀 崇由, 服部 淳彦

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      2011年4月 - 2015年3月

      課題番号:23570091

      配分額:5980000円 ( 直接経費:4600000円 、 間接経費:1380000円 )

      本研究は動物の生殖リズムが社会的環境により如何に調節されるかを明らかにすることを目的とした。生殖リズムを調節する最も大きな要因は明暗の光サイクルであるが、生殖は本来社会的活動であるから同性や異性からの社会的刺激からも大きな影響を受ける。本研究では短日条件下にあるために非繁殖の状態にあるウズラに雄の鳴声を長日条件と同様に長時間聞かせると発現が著しく高まる遺伝子が同定された。その遺伝子産物の局在を調べたところ、中脳聴覚野に存在するニューロン群が同定された。雄ウズラの鳴声はこれらニューロン群で情報処理され、GnRHニューロンに伝えられ、明暗の光サイクルと無関係にLHの分泌を制御するものと考えられる。

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    • 魚鱗の再生と両生類変態期の後肢形成を骨形成モデルとしたメラトニンの制御機構の解明

      文部科学省/日本学術振興会 

      服部 淳彦, 田渕 圭章, 服部 淳彦

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      2012年 - 2015年

      担当区分:研究代表者  資金種別:競争的資金

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    • 魚鱗の再生と両生類変態期の後肢形成を骨形成モデルとしたメラトニンの制御機構の解明

      文部科学省/日本学術振興会 

      服部 淳彦, 田渕 圭章, 服部 淳彦

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      2012年 - 2014年

      担当区分:研究代表者  資金種別:競争的資金

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    • 各種骨関連手術後の治癒期間短縮を目指したメラトニン療法の開発

      日本学術振興会  科学研究費助成事業 

      里村 一人, 舘原 誠晃, 徳山 麗子, 山本 英雄, 服部 淳彦, 橘 竜佑, 工藤 景子, 湯浅 哲也, 神谷 洋子

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      2009年 - 2011年

      課題番号:21592575

      配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

      概日リズム調節ホルモンであるメラトニンが、骨芽細胞に作用することを利用し、本研究では、骨折後にメラトニンを投与するとその治癒期間が短縮されることを見出すとともに、チタン製インプラント埋入後にメラトニンを投与するとosseointegrationが促進されることを確認した。

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    • 再生鱗における隆起線形成の日周性と時計遺伝子との関連:メラトニンによる制御

      日本学術振興会  科学研究費助成事業 

      服部 淳彦, 鈴木 徹

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      2009年 - 2011年

      課題番号:21570062

      配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

      硬組織には、約1日を単位として形成される日周輪(成長線)の存在が古くから知られているが、その日周輪形成の制御機構を明らかにした研究は無い。そこで本研究では、キンギョの再生鱗を用いて、それらを明らかにする実験を行った。まず、キンギョの鱗の再生過程において我々が単離した骨芽細胞関連遺伝子の発現を調べたところ、哺乳類の膜性骨の再生過程で発現する遺伝子のパターンとほぼ同じパターンを示すことが分かった。次に、再生鱗を用いて、隆起線(成長線)が1日1本ずつ外側に形成されていくこと、また、時計遺伝子の中では少なくともper-3の発現に明瞭な概日リズムが認められることを明らかにした。

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    • 象牙質における成長線の周期性と生物時計との関連:メラトニンによる調節

      日本学術振興会  科学研究費助成事業 

      三島 弘幸, 田畑 純, 鈴木 信雄, 服部 淳彦, 鈴木 信雄, 服部 淳彦

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      2008年 - 2010年

      課題番号:20592168

      配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

      ラットの切歯と臼歯の歯胚組織において、メラトニンのレセプターMT1とMT2は発現していることが判明した。夜間が昼間より発現量が高かった。夜間時の石灰化前線にヘマトキシレンに濃染する層が観察された。夜間に石灰化が進行し、ヘマトキシレンに濃染する層が形成され、昼間にヘマトキシレンに淡染する層が形成され、濃染層と淡染層が対になった成長線になると考察される。成長線の周期性にメラトニンが関与する可能性が示唆された。

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    • クサフグの月周同調産卵回遊を制御する神経内分泌基盤の解明

      日本学術振興会  科学研究費助成事業 

      安東 宏徳, 服部 淳彦

      詳細を見る

      2008年 - 2010年

      課題番号:20570058

      配分額:4810000円 ( 直接経費:3700000円 、 間接経費:1110000円 )

      クサフグの月周に同調した産卵回遊行動を調節する脳内機構を明らかにするため,間脳の視床下部神経内分泌系と松果体の働きを解析した。視床下部の生殖中枢から分泌される複数の神経ホルモンの合成は,産卵期に著しく高まると共に明瞭な日周変動を示した。この日周リズムは,松果体から周期的に分泌されるメラトニンによって調節されることが示唆された。また,松果体のメラトニン受容体の合成は14時間周期のリズムを持つと共に,月周に依存した変動を示すことが分かった。さらに,産卵期のクサフグは水位を一定にした水槽内でも月周に同調して集合することが分かり,松果体で駆動される内因性の月周リズムを持つことが示唆された。

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    • 各種アミノ酸およびDNAメチル化に関与する食事因子とがん罹患に関するコホート研究

      日本学術振興会  科学研究費助成事業 

      永田 知里, 清水 弘之, 服部 淳彦

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      2008年 - 2009年

      課題番号:20014006

      配分額:9400000円 ( 直接経費:9400000円 )

      本研究は、各種アミノ酸やDNAメチル化に関与するメチオニン、葉酸、ビタミンB6、B12の食事からの摂取推定を可能とし、がん罹患との関連性を一般住民における前向き研究のデザインで評価することを目的としている。前年度では、岐阜県のがん登録および高山市医師会の飛騨がん登録より、高山市に1992年に開始されたコホート(高山コホート)内におけるがん罹患データを得たが、コホート内における死亡者、転居者の情報が把握出来ず、本年度、高山市での住民票閲覧、除票情報の提供、法務局への戸籍閲覧などを依頼した。未だ一部の情報が入手できておらず、また国の食品成分委員会による各食品中アミノ酸含有量改訂発表が遅れているが、まず、葉酸、ビタミンB6、B12摂取と大腸がんと乳がん罹患についての関連性を評価した。対象者は1992年9月前向き研究開始時にがん既往があると回答した者あるいはがん登録情報からこの時点でがんに罹患していたことが判明した者を除き、男性14,185名、女性16,560名であった。2005年末までの期間に新しく大腸がんと診断された者は男性277名、女性233名、乳がん罹患(女性のみ)は134名であった。年齢、喫煙歴、BMI、アルコール摂取量で補正後、男性におけるビタミンB12の上位1/3の高摂取群は下位1/3の低摂取群に比べ大腸がんハザード比が1.39と統計的に有意に高く(p=0.03)、またビタミンB6摂取も高摂取群はハザード比が1.37 (p=0.056)と高かった。しかし、これらの関連性は肉・肉加工品類摂取の補正により低下した。大腸がんリスクと葉酸摂取との関連性は認められなかった。女性では大腸がん、乳がんともこれらの栄養因子との関連は有意でなかった。多重共線性の問題に配慮しつつ、アミノ酸を含み、各栄養素、食品群の交絡の影響もさらに考慮する必要があると考えられる

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    • 迅速・高感度・簡便な新規骨粗鬆症モデル系の開発と予防法への応用

      日本学術振興会  科学研究費助成事業 

      北村 敬一郎, 根本 鉄, 服部 淳彦

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      2006年 - 2008年

      課題番号:18650197

      配分額:2900000円 ( 直接経費:2900000円 )

      [研究目的]魚類のウロコには骨形成をする骨芽細胞、骨吸収をする破骨細胞およびコラーゲンを中心とする骨基質タンパクからなり、健常骨モデル、骨代謝亢進モデルおよび骨吸収亢進モデル等の骨のモデルが作成できることを既に報告している。昨年は、これらの骨モデルに機械的刺激である低強度超音波を照射しその効果を調べた。その結果、健常骨モデルおよび骨代謝亢進モデルでは、骨形成が増加し、骨代謝亢進モデルでは、さらに骨吸収が抑制される効果も明らかとなった。一方、炎症性の骨吸収亢進モデルでは、骨形成および骨吸収作用に変化がないことが明らかとなった。今年度は、ウロコモデルで確認された低強度超音波の効果をマウスの頭蓋骨を使い、哺乳類の骨代謝に対する低強度超音波の効果を調べた。
      [研究成果]マウス新生仔をエーテル過剰吸入麻酔後、断頭し、頭蓋骨を取り出し左右を2等分した2骨片を対照群と曝露群とした。ウロコの系で決定した強度の低強度超音波を曝露群に照射後、対照群と同様に24時間組織培養し、超音波破砕し上清の酵素活性を測定した。その結果、骨形成をする骨芽細胞活性および骨吸収をする破骨細胞活性のどちらも変化しなかった。哺乳類の骨細胞は、骨基質内に存在しているため超音波が反射され届かないためと思われた。できるだけ骨組織が薄く柔らかいマウスの新生仔頭蓋骨を使ったが、細胞が表面にいるウロコとはやはり大きく異なることが明らかとなった。したがって、哺乳類の骨代謝を改善するためには、骨基質内にある細胞にも到達しうる機械的刺激が必要なことが示唆された。また、超音波の骨代謝への効果を調べるには、骨基質の表面に骨芽細胞と破骨細胞が共存するウロコが適したモデルとなることが明らかとなった。

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    • 魚鱗を用いた破骨-骨芽細胞相互の分化制御遺伝子の網羅的単離とメラトニンによる制御

      日本学術振興会  科学研究費助成事業 

      服部 淳彦

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      2006年 - 2008年

      課題番号:18570055

      配分額:4000000円 ( 直接経費:3400000円 、 間接経費:600000円 )

      硬骨魚類の鱗を骨のモデルとしてとらえ、破骨細胞の分化に関わる遺伝子(NFATc-1)や機能遺伝子(TRAP, Cathepsin K, MMP-9)をキンギョの鱗からクローニングし、それらの遺伝子が鱗の破骨細胞で発現していることを明らかにした。また、破骨細胞の分化に関わるRANKとRANKL遺伝子のクローニングに成功した。最終年度では、初年度に確立した筋肉内への鱗の自家移植(破骨細胞誘導)の系を用いて、メラトニンが多核の成熟破骨細胞で発現している機能遺伝子を抑制するだけでなく、破骨細胞の分化に関わるRANKとRANKLの遺伝子の発現に対しても抑制することにより、破骨細胞の分化も抑制することを明らかにした。

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    • ホルモン合成と放出のバランス制御機構:単-LH細胞を用いた解析

      日本学術振興会  科学研究費助成事業 

      安東 宏徳, 服部 淳彦, 西村 正太郎

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      2006年 - 2007年

      課題番号:18570060

      配分額:4050000円 ( 直接経費:3600000円 、 間接経費:450000円 )

      本研究の目的は,単一LH細胞を用いてLHの合成と放出のバランスをとる分子メカニズムを明らかにすることである。まず始めに,溶血プラークアッセイ法を用いてサクラマスの下垂体初代培養細胞から単一LH細胞を同定すると共に,LH放出活性の定量系を確立した。また,単一LH細胞のLH合成活性を測定するため,LHβサブユニットのmRNA定量系をリアルタイムPCR法を用いて確立した。最小検出感度は約100コピーであり,単一細胞中のLHβサブユニットmRNAの定量には十分の感度を持っていた。また,遺伝子の転写活性を測定する系として,ヘテロ核RNAを検出するリアルタイムPCR系を検討した。単一細胞のヘテロ核RNAの測定には最小検出感度に近いレベルでの精度のよい測定が必要であることが分かり,より精度よく安定して測定できるようにさらなる条件検討を行うことが必要である。
      サクラマスを非産卵期と産卵期前に採集し,単一LH細胞の合成と放出の解析系を用いて性成熟段階の異なる魚におけるバランス制御系の機能の違いを調べた。溶血プラークアッセイによって同定されたLH細胞の数は,非産卵期の魚は産卵期前の魚に比べて少なく,放出活性が低かった。また,リアルタイムPCR法によって測定したLHβサブユニットのmRNAも非産卵期では低かった。しかし,GnRHに対する反応性を調べると,非産卵期ではGnRH投与により放出と合成の活性が上昇したが,産卵期前では放出は高まったが合成は変化しなかった。産卵期前ではLHβサブユニット遺伝子の発現調節に関わる細胞内シグナル伝達系のGnRH応答性が変化することが示唆された。次に,ディファレンシャルディスプレイ法の一つであるGeneFishing法を用いて,両時期のLH細胞の間で発現量の異なる遺伝子の探索を行ったが,これまでのところ候補遺伝子は得られていない。使用した任意プライマーの数が少ないためと考えられる。

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    • 血清タンパク質の測定による野生鳥類の内分泌攪乱評価法の研究

      日本学術振興会  科学研究費助成事業 

      和田 勝, 奈良 雅之, 岡崎 三代, 服部 淳彦

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      2001年 - 2003年

      課題番号:13027220

      配分額:14700000円 ( 直接経費:14700000円 )

      野生鳥類における内分泌撹乱の現状を評価し、汚染との関係を明らかにする方法を確立するため、血中ビテロジェニンを指標にしてエストロジェン様攪乱を評価することにし、ウズラの血中ビテロジェニンを測定できるELISA系が確立した。これが野生鳥類に適用できるかを明らかにするため、キンカチョウを使ってデータを収集した。
      はじめに、キンカチョウの卵黄タンパク質をSDS-PAGEで泳動をしてウズラの卵黄タンパク質の泳動パターンと比較し、さらに、ウズラ卵黄リポビテリンを抗原としてウサギで作成したポリクローナル抗体との交叉を確認するために、ウエスターンブロッティング法で確認した。SDS-PAGEによる泳動パターンの比較とウエスターンブロッティングの結果は、キンカチョウ卵黄内に、ウズラと同じように120kDa付近に二本のバンドが抗体で認識された。
      キンカチョウ卵黄中にはウズラのリポビテリン抗体に交叉するリボビテリンが存在することが確認できたので、雄キンカチョウにエストラジオールを100g体重あたり0.1から公比10で1000ugまでの5段階の濃度でコーンオイル中に溶解し、7日間腹腔中に注射によって投与した。対照群はコーンオイルのみを投与した。7日間投与した後、血液を採取し、血清中のビテロジェニンをウズラビテロジェニンモノクローナル抗体によるELISAで測定し、血中エストラジオールも測定した。ビテロジェニン測定系の感度は検出限界0.0125ug/ml、エストラジオールは40pg/mlであった。血液採取後に、体重を測定し、肝臓、精巣の湿重量を測定した。体重はおよそ10gから15gなので、肝臓と精巣は10g体重あたりに換算した。上記の濃度を7日間投与したキンカチョウの肝臓の重量は群間で有意な差が認められず、精巣も1000ug投与群で減少傾向がみられたが、有意な差はなかった。エストラジオールの投与によって雄キンカチョウでも血中ビテロジェニン濃度が増加したが、0.1から10ugでは増加は認められなかった。血中のエストラジオールは1ug投与群で上昇しているが、血中ビテロジェニンの増加は認められなかった。
      ウズラではエストラジオール0.1ug投与で反応する個体が認められ、1ugではビテロジェニンの明らかな増加が認められるのに対して、キンカチョウではウズラに比べて明らかに反応性が低かった。ウズラのように離巣性で卵への投資量が大きな鳥類と、比較的卵への投資量が少ない就巣性の鳴禽類では、肝臓からのビテロジェニンの供給が異なることを示しているように思われた。野生鳥類での評価は、この点を考慮する必要があるだろう。

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    • 松果体除去による脊柱変形のメカニズムと松果体に内在する生物時計の役割

      日本学術振興会  科学研究費助成事業 

      服部 淳彦

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      2000年 - 2002年

      課題番号:12640647

      配分額:3600000円 ( 直接経費:3600000円 )

      孵化後すぐにニワトリの松果体を除去すると,脊柱形成に異常が生じ,2週間後には100%の個体に側弯が生じる。この実験モデルは脊柱形成過程を時間・空間レベルで調べることができる優れたモデルである。そこで本研究は、松果体除去による脊柱側彎変形の発生原因を探るために,1)急激な体重増加がもたらす座屈の可能性と,2)脊柱変形が生じる前に椎骨の前後方向において成長差が生じている可能性を調べた。また合わせて,松果体に内在する体内時計が脊柱変形に関わる可能性についても実験を行った。
      その結果,孵化後1日目に松果体を除去すると100%の個体に脊柱変形が生じるのに対して,5日目に松果体を除去すると80%,2週目に除去すると55%,3週目になると約20%の個体にしか脊柱の変形が発生しなかった。また,その時の松果体内の概日時計について調べるために,恒明条件下における血中メラトニン濃度の日内変化を測定した。その結果,1,3日齢では明瞭な概日リズムが認められたのに対して,3週齢ではリズムは消失していた。このことは脊柱変形の原因として松果体に内在する時計の重要性を示唆するものである。次に松果体除去後ニワトリを3群に分け、100%、50%,25%体重群を作成して脊柱の変形度を調べた。その結果、100%体重群では平均50度の脊柱変形度を示したのに対して,50%体重群では38度に,また25%体重群では13度にまで減少した。また,脊柱が曲がり始める前の4日令に椎骨を採取し,椎骨の前後の成分における骨化度を組織学的手法を用いて比較したところ,前方部分には両群で差がなかったのに対して,後方部分では松果体除去群の方が対照群に比べ有意に骨化が進んでいることが明らかとなった。これらの結果は,脊推の前後成分における成長の不均衡と急激な荷重による座屈現象が脊柱変形の発生要因である可能性を示すものである。

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    • 松果体除去ニワトリに多発する脊柱変形と骨形成におけるメラトニンの役割

      日本学術振興会  科学研究費助成事業 

      服部 淳彦

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      1997年 - 1998年

      課題番号:09640788

      配分額:3100000円 ( 直接経費:3100000円 )

      脊椎動物の身体を支える中軸骨格である脊柱がいかに形作られていくかは,非常に興味深いテーマである。現在初期発生に関しては,主としてホメオボックス遺伝子群の解析といった研究が盛んに行われているものの,いったんできた場にどのようなメカニズムにより,全体としてバランスを取りながら軟骨・骨が形成されて,正しい脊柱が形作られていくのかは,ほとんど分かっていない。本研究で用いている松果体除去ニワトリ脊柱変形モデルは,脊柱形成過程の後期のメカニズムを,個体レベルで調べるのには非常に優れたモデルである。我々はこれまで卵用品種を用いて脊柱変形のメカニズムを調べてきたが,松果体除去2ヶ月後でも約7割の個体にしか脊柱変形が認められないことから,よりよいモデルを捜し出す必要性が考えられた。そこで今回,肉用品種(ブロイラー)を用いて同様の検討を行ったところ,孵化後2日以内に除去手術を行うと,2週令で100%の個体に脊柱変形が認められた。また,メラトニンの投与は,脊柱変形の発生率を減少させ,進行も有意に抑制した。そこで次に,ブロイラーに脊柱変形が多発した原因を明らかにするために,体重増加と脊柱変形との関連を調べた。60%体重群,80%体重群を作製し,対照群と比較したが,脊柱変形の発生率や変形度に有意な差はなく,この松果体除去後に生じる脊柱変形はブロイラーに本質的に生じやすいことを明らかにした。次に,脊椎骨と血液中のCa濃度を調べたところ,松果体除去後比較的初期の段階で骨Ca濃度は有意に減少し,一方血中Ca濃度は有意に上昇することが分かった。そこで飼料に約3倍量のCaを添加し,松果体除去後の脊柱変形を調べたところ,有意に抑制された。以上のことは,松果体除去後に発生する脊柱変形の原因の一部にCa代謝が関与していることを示している。

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    • ホルモン遺伝子発現に関わるZnクラスタータンパク質・メタロチオネイン

      日本学術振興会  科学研究費助成事業 

      服部 淳彦

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      1995年 - 1996年

      課題番号:07804058

      配分額:2200000円 ( 直接経費:2200000円 )

      分化の基本骨格は,主としてある特定遺伝子の転写レベルの制御にある。この転写レベルの制御には,転写因子と呼ばれる一連のタンパク質が関与し,中でもステロイドホルモンのレセプターをはじめとする核内レセプターは,分子内にZnフィンガー構造をとる特徴的なタンパク質であり,金属であるZnが重要な役割を担っている。一方,メタロチオネイン(MT)は正常組織でもZnクラスタータンパク質として存在することが知られ,核内のレセプターとの間で何らかの機能関連が推測される。そこで,本研究では核内に出現するMTがいかなる働きをしているかを明らかにするために,正常ラット下垂体を用いて,各種ホルモン産生細胞とMTとの共存を免疫組織化学的手法および in situ hybridization法を用いて調べた。60日齢雄ラット1匹当たり5000〜9000個のGH細胞を調べたところ,0.44±0.15%の細胞の核においてMTが陽性であった。一方他のホルモン産生細胞においては,どの細胞もMTが共存している像は認められなかった。ラットGH産生細胞株であるMtT/Sに,E2,EGFおよびinsulinを複合作用させると,24時間目に急激にMTが発現し,それと時を同じくしてGHmRNA陽性細胞は有意に(p<0.01)減少し,PRLmRNA陽性細胞は有意に(p<0.01)増加することから,正常ラット下垂体における核内MT陽性GH細胞は、PRL細胞に変化する前段階の細胞である可能性が示唆された。現在胎仔期の下垂体GH細胞およびPRL細胞とMTとの関連を調べている。

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    • 下垂体前葉に新たに見いだされたメタロチオネインのホルモン遺伝子発現との関連

      日本学術振興会  科学研究費助成事業 

      服部 淳彦

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      1994年 - 1994年

      課題番号:06857004

      配分額:900000円 ( 直接経費:900000円 )

      メタロチオネインは,Znなどの重金属に結合する蛋白質であり,近年本蛋白質の核内における機能が注目されている。本研究では,ラット下垂体細胞株であるMtT/SにE2.INSおよびEGFを複合添加し,in situ hybridization法によるGHおよびPRLmRNA発現と免疫染色によるメタロチオネイン発現を経時的に比較した。
      メタロチオネインは,3種ホルモン添加後18時間まで対照群と同様にほとんど発現していなかったが,24時間目で急激に発現し(P<0.001).約40%の細胞が陽性像を示した。しかも,メタロチオネイン陽性細胞のうちの約80%において核のみにその存在が認められた。GHmRNA陽性細胞は3種ホルモン添加前には約60%に存在したが,添加後24時間から有意に(P<0.01)減少し,72時間では20%以下に減少した。一方,PRLmRNA陽性細胞は添加前にほとんど認められなかったが,添加後24時間から有意に(P<0.05)増加し,72時間には約5%の細胞が陽性像を示した。以上の結果は,核内に発現したメタロチオネインがホルモン遺伝子発現に関与する可能性を示唆するものである。

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    • メラトニンおよび食材に含まれるメラトニン様物質の抗酸化効果

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      資金種別:競争的資金

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    • 骨代謝疾患における時間生物学的治療薬の開発

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      資金種別:競争的資金

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    • 加齢性記憶障害とメラトニン

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      資金種別:競争的資金

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    • 破骨・骨芽細胞の分化および機能解明-硬骨魚類のウロコを骨のモデルとして

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      資金種別:競争的資金

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    • ホルモンの機能進化とそれを支える分子的基盤に関する研究

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      資金種別:競争的資金

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    • Chronobiological study on developing the spine

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      資金種別:競争的資金

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    • Study on the molecular aspects of evolution in the hormone and its function

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      資金種別:競争的資金

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    • 骨芽細胞で作られるホルモンによる破骨細胞の調節機構:骨モデル(ウロコ)による解析

      文部科学省/日本学術振興会 

      鈴木 信雄, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 時刻情報伝達物質であるメラトニンによる象牙質の組織構造と象牙芽細胞の制御機構

      文部科学省/日本学術振興会 

      三島弘幸, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 学習記憶機能におけるメラトニンの抗加齢効果の解析

      文部科学省/日本学術振興会 

      千葉 篤彦, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 学習記憶機能におけるメラトニンの抗加齢効果に関する作用機序の解明

      文部科学省/日本学術振興会 

      千葉 篤彦, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • メラトニンと骨代謝調整ホルモン・サイトカインとの新規相互作用の解明

      文部科学省/日本学術振興会 

      池亀 美華, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 新規体内時計と環境因子によるクサフグの半月周性産卵リズムの形成機構

      文部科学省/日本学術振興会 

      安東 宏徳, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 食事由来のメラトニン:摂取量推定と閉経およびがん・循環器疾患との関連

      文部科学省/日本学術振興会 

      永田 知里, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 象牙質の成長線の周期と体内時計の情報伝達分子のメラトニンの分泌リズムとの関係

      文部科学省/日本学術振興会 

      三島 弘幸, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 硬骨を持たない原始的脊椎動物ヤツメウナギにおける新規カルシウム代謝機構の解明

      文部科学省/日本学術振興会 

      関口 俊男, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 白内障手術の全身的健康指標の改善効果

      文部科学省/日本学術振興会 

      綾木 雅彦, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 骨組織におけるメラトニンと機械的刺激の相互作用の解明

      文部科学省/日本学術振興会 

      池亀 美華, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 硬骨を持たない原始的脊椎動物ヤツメウナギにおける新規カルシウム代謝機構 の解明

      文部科学省/日本学術振興会 

      関口 俊男, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • 新規硬組織モデルを用いた微小重力・過重力下での骨吸収及び骨形成の機構解析

      文部科学省/日本学術振興会 

      鈴木 信雄, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    • メラトニンと骨代謝調節ホルモン・サイトカインとの新規相互作用の解明

      文部科学省/日本学術振興会 

      池亀 美華, 服部 淳彦

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      担当区分:連携研究者  資金種別:競争的資金

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    ▼全件表示

    産業財産権

    • 長期記憶誘導剤

      服部 淳彦, 松本 幸久, 影近 弘之, 増野 弘幸, 丸山 雄介

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      出願人:国立大学法人東京医科歯科大学、学校法人上智学院

      出願番号:15/593,571  出願日:2017年5月12日

      公開番号:2018-0327348-A1  公開日:2018年11月15日

      特許番号/登録番号:10266482 

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    • 胎盤抽出物の製造方法

      服部 淳彦, 丸山 雄介, 宮内 大治, 横川 剛, 高野 裕二朗

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      出願人:国立大学法人東京医科歯科大学、株式会社協和

      出願番号:特願2015-234222  出願日:2015年11月30日

      公開番号:特開2017-10097  公開日:2017年6月8日

      特許番号/登録番号:特許第6769705号 

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    • 長期記憶誘導剤

      服部 淳彦, 松本 幸久, 影近 弘之, 増野 弘幸, 千葉 篤彦, 岩下 洸

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      出願人:国立大学法人東京医科歯科大学、学校法人上智学院

      出願番号:特願2016-042875  出願日:2016年3月4日

      公開番号:特開2016-166197  公開日:2016年9月15日

      特許番号/登録番号:特許第6836044号 

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    社会貢献活動

    • 老化スイッチ:体内時計の乱れが老化を加速する!

      取材協力

      日本経済新聞社  日経Gooday  2018年1月5日

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      対象: メディア

      種別:新聞・雑誌

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    • ノーベル賞で注目の体内時計、リズムを作るメラトニンを受け取る受容体と呼ばれるたんぱく質

      取材協力

      日本経済新聞社  日経Gooday  2017年12月5日

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      対象: メディア

      種別:新聞・雑誌

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    • 私たちの体・心・肌は1日のリズムで変化しています

      情報提供

      文化出版局  ミセス  2017年8月7日

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      対象: 社会人・一般

      種別:会誌・広報誌

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    • 1日のリズムを意識する「時間美容」のすすめ

      取材協力, 情報提供

      小学館  美的  2017年3月23日

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      対象: 社会人・一般

      種別:会誌・広報誌

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